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The map label for this gene is hmeA [H]

Identifier: 78221547

GI number: 78221547

Start: 362444

End: 363226

Strand: Direct

Name: hmeA [H]

Synonym: Gmet_0326

Alternate gene names: 78221547

Gene position: 362444-363226 (Clockwise)

Preceding gene: 78221546

Following gene: 78221548

Centisome position: 9.07

GC content: 63.47

Gene sequence:

>783_bases
ATGAACAGGCGAGACTTTCTCAAGAATAGCATCACGGTCATCGCGGGGCTGGCGGTGCCCCTCTCGGCCCTGGAACTCAT
CAGCCCGAGGAAGCTCCTGGCTCAAAAGGGAGACGAATCGCCGGTCCGGTGGGCCTTTCTGGTGGACACCCAGGCCTGCG
TCGGGTGCGGCTTCTGCGTCAAGGCGTGCAAGACGGAGAACGAGATCCCCCACGAGACCAACATCACCCGCACCTGGGTG
GAACGGTACGTGGTGACCCGGAAGGGGAAGACCTACGTGGATTCTCCCCGGGGAGCCCGGGACGGCTTCACCACGGACCG
GATCGATCAGGGCGAGGAAGGGTATCGCGAGGTCAAGGCGAAGGATGTCGAGAAGGCGTTCTTCGTCCCCAAGCTCTGCA
ACCAGTGCGATAACCCCGCCTGCGTCCAGGTCTGCCCCGTGGGAGCCACCTACCAGACCCCCGACGGCGTGGTGCTGGTG
GACCGGAAGCACTGCATCGGCTGCGCCTACTGCATCATGGCGTGCCCCTACGGGGTCCGCTCCTTCAATCCGGCCTACAA
GACGGCGGAGAAGTGCACCTTCTGCTATCACCGGATCAGTAAGGGGATGAAGACGGCCTGCGTGGACGCCTGCCCCTTCG
GTGCCCGGAAGATCGGCAACCTGCGGGATCCCGACGATCCCGTGACGAAGACCATCATGTCGGGACGGGTCGGCGTCCTG
AAGGAGCAGTACGGCACCAAGCCGCAGGTCTACTATCTCGCTCTTTCCCGGGAGGTGAAATAG

Upstream 100 bases:

>100_bases
AAGCGAGGCGTACCCGTTGGTACGTTGAGTTTTCGAGTGAGCGGCAACAACGCAGACCGGCCCCGCAGTAGTTTTGCATG
AGCCTCAAGGAGTCAAAGCA

Downstream 100 bases:

>100_bases
CCATGGTACACGGTGAAGCATGGACGCTGAAAGAGTTGTTCGTCTACCCCAACGAGTACATCTACTGGACCATCCAGATC
GTCATGTACCCGTTCATGAC

Product: twin-arginine translocation pathway signal

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNRRDFLKNSITVIAGLAVPLSALELISPRKLLAQKGDESPVRWAFLVDTQACVGCGFCVKACKTENEIPHETNITRTWV
ERYVVTRKGKTYVDSPRGARDGFTTDRIDQGEEGYREVKAKDVEKAFFVPKLCNQCDNPACVQVCPVGATYQTPDGVVLV
DRKHCIGCAYCIMACPYGVRSFNPAYKTAEKCTFCYHRISKGMKTACVDACPFGARKIGNLRDPDDPVTKTIMSGRVGVL
KEQYGTKPQVYYLALSREVK

Sequences:

>Translated_260_residues
MNRRDFLKNSITVIAGLAVPLSALELISPRKLLAQKGDESPVRWAFLVDTQACVGCGFCVKACKTENEIPHETNITRTWV
ERYVVTRKGKTYVDSPRGARDGFTTDRIDQGEEGYREVKAKDVEKAFFVPKLCNQCDNPACVQVCPVGATYQTPDGVVLV
DRKHCIGCAYCIMACPYGVRSFNPAYKTAEKCTFCYHRISKGMKTACVDACPFGARKIGNLRDPDDPVTKTIMSGRVGVL
KEQYGTKPQVYYLALSREVK
>Mature_260_residues
MNRRDFLKNSITVIAGLAVPLSALELISPRKLLAQKGDESPVRWAFLVDTQACVGCGFCVKACKTENEIPHETNITRTWV
ERYVVTRKGKTYVDSPRGARDGFTTDRIDQGEEGYREVKAKDVEKAFFVPKLCNQCDNPACVQVCPVGATYQTPDGVVLV
DRKHCIGCAYCIMACPYGVRSFNPAYKTAEKCTFCYHRISKGMKTACVDACPFGARKIGNLRDPDDPVTKTIMSGRVGVL
KEQYGTKPQVYYLALSREVK

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=252, Percent_Identity=32.9365079365079, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI226510944, Length=199, Percent_Identity=33.1658291457286, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1787122, Length=209, Percent_Identity=30.1435406698565, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787872, Length=209, Percent_Identity=29.6650717703349, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1790326, Length=198, Percent_Identity=31.3131313131313, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787478, Length=144, Percent_Identity=36.8055555555556, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789370, Length=226, Percent_Identity=31.858407079646, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787740, Length=148, Percent_Identity=37.8378378378378, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1787749, Length=180, Percent_Identity=29.4444444444444, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787963, Length=227, Percent_Identity=27.3127753303965, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082180, Length=89, Percent_Identity=40.4494382022472, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI2367245, Length=85, Percent_Identity=40, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 28915; Mature: 28915

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
6.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRDFLKNSITVIAGLAVPLSALELISPRKLLAQKGDESPVRWAFLVDTQACVGCGFCV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHH
KACKTENEIPHETNITRTWVERYVVTRKGKTYVDSPRGARDGFTTDRIDQGEEGYREVKA
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
KDVEKAFFVPKLCNQCDNPACVQVCPVGATYQTPDGVVLVDRKHCIGCAYCIMACPYGVR
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCEEEECCCHHHHHHHHHHHCCCCCC
SFNPAYKTAEKCTFCYHRISKGMKTACVDACPFGARKIGNLRDPDDPVTKTIMSGRVGVL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHCCHHHH
KEQYGTKPQVYYLALSREVK
HHHHCCCCEEEEEEECCCCC
>Mature Secondary Structure
MNRRDFLKNSITVIAGLAVPLSALELISPRKLLAQKGDESPVRWAFLVDTQACVGCGFCV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHH
KACKTENEIPHETNITRTWVERYVVTRKGKTYVDSPRGARDGFTTDRIDQGEEGYREVKA
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
KDVEKAFFVPKLCNQCDNPACVQVCPVGATYQTPDGVVLVDRKHCIGCAYCIMACPYGVR
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCEEEECCCHHHHHHHHHHHCCCCCC
SFNPAYKTAEKCTFCYHRISKGMKTACVDACPFGARKIGNLRDPDDPVTKTIMSGRVGVL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHCCHHHH
KEQYGTKPQVYYLALSREVK
HHHHCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]