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The map label for this gene is nrfH [H]

Identifier: 78221518

GI number: 78221518

Start: 331483

End: 331944

Strand: Reverse

Name: nrfH [H]

Synonym: Gmet_0295

Alternate gene names: 78221518

Gene position: 331944-331483 (Counterclockwise)

Preceding gene: 78221527

Following gene: 78221517

Centisome position: 8.3

GC content: 61.04

Gene sequence:

>462_bases
ATGGGATTGCCCGGGACCGGAAACCAGGCCATCTACCTGGCGATCGGCGTAATCGTAGCAGCGGTGGCGCTCTTCGTGCT
TTTCGGGCCGCCGAAGCTGCTGGCCAAATCGGACACCCCCGACTTCTGCGTCGGGTGCCACCCCATGGAAAATGAATACG
AGGCATGGATCCACACCGGTGCCCACCGGCGGGTCAAGTGCGTCGACTGCCACCTCCCCAACGAGAACCTCGCGGTCCAC
TATATCTGGAAATCCATCGACGGCGCGAAGGACGCCATCTTCTTCTATTCGGGACACGTGCCGGAGATCATCAGGCTCTC
GAAGCACGGAGAAGAGGTTCTCCAGAAGAACTGCGTCCGCTGCCACGACACCACCGTGACCCACATCGATACCGAACGCC
AATGCTGGAAATGCCACCGCCGCGTCTCCCACACGCGGAGCGGGGCCATGGAAACCTTATAG

Upstream 100 bases:

>100_bases
TCAAGGGGAAGAATTTCACCGTTGCGAGGCGAAGATTGGAAGATATACTGTTAGTCACATATCATTACAATCGGCACATC
ACCAAGAGGAGGTGAACGAT

Downstream 100 bases:

>100_bases
GAGGCTCAACAATGAGACGACTGAATCCTGTTGCACCCCTGATCGCGCTGGCTGCCGCGGCAACCCTGTTTGTCCTCGGG
GGATGCGCCCCGGAGAAAGC

Product: respiratory nitrite reductase specific menaquinol--cytochrome-c reductase (NrfH) precursor

Products: H2O; trimethylamine; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MGLPGTGNQAIYLAIGVIVAAVALFVLFGPPKLLAKSDTPDFCVGCHPMENEYEAWIHTGAHRRVKCVDCHLPNENLAVH
YIWKSIDGAKDAIFFYSGHVPEIIRLSKHGEEVLQKNCVRCHDTTVTHIDTERQCWKCHRRVSHTRSGAMETL

Sequences:

>Translated_153_residues
MGLPGTGNQAIYLAIGVIVAAVALFVLFGPPKLLAKSDTPDFCVGCHPMENEYEAWIHTGAHRRVKCVDCHLPNENLAVH
YIWKSIDGAKDAIFFYSGHVPEIIRLSKHGEEVLQKNCVRCHDTTVTHIDTERQCWKCHRRVSHTRSGAMETL
>Mature_152_residues
GLPGTGNQAIYLAIGVIVAAVALFVLFGPPKLLAKSDTPDFCVGCHPMENEYEAWIHTGAHRRVKCVDCHLPNENLAVHY
IWKSIDGAKDAIFFYSGHVPEIIRLSKHGEEVLQKNCVRCHDTTVTHIDTERQCWKCHRRVSHTRSGAMETL

Specific function: Subunit of the nitrite reductase complex that mediates electron transfer from quinol to the catalytic subunit [H]

COG id: COG3005

COG function: function code C; Nitrate/TMAO reductases, membrane-bound tetraheme cytochrome c subunit

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017571
- InterPro:   IPR011031
- InterPro:   IPR005126 [H]

Pfam domain/function: PF03264 Cytochrom_NNT [H]

EC number: NA

Molecular weight: Translated: 17148; Mature: 17017

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLPGTGNQAIYLAIGVIVAAVALFVLFGPPKLLAKSDTPDFCVGCHPMENEYEAWIHTG
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCEEEECCCCCCHHHHHHCCC
AHRRVKCVDCHLPNENLAVHYIWKSIDGAKDAIFFYSGHVPEIIRLSKHGEEVLQKNCVR
CCCEEEEEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCE
CHDTTVTHIDTERQCWKCHRRVSHTRSGAMETL
ECCCEEEEECCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GLPGTGNQAIYLAIGVIVAAVALFVLFGPPKLLAKSDTPDFCVGCHPMENEYEAWIHTG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCEEEECCCCCCHHHHHHCCC
AHRRVKCVDCHLPNENLAVHYIWKSIDGAKDAIFFYSGHVPEIIRLSKHGEEVLQKNCVR
CCCEEEEEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCE
CHDTTVTHIDTERQCWKCHRRVSHTRSGAMETL
ECCCEEEEECCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: trimethylamine-N-oxide; NADH [C]

Specific reaction: trimethylamine-N-oxide + NADH = H2O + trimethylamine + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10672190; 14500908 [H]