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The map label for this gene is ppc [H]

Identifier: 78221527

GI number: 78221527

Start: 339632

End: 342427

Strand: Reverse

Name: ppc [H]

Synonym: Gmet_0304

Alternate gene names: 78221527

Gene position: 342427-339632 (Counterclockwise)

Preceding gene: 78221535

Following gene: 78221518

Centisome position: 8.57

GC content: 67.95

Gene sequence:

>2796_bases
ATGGCGGAACGAGAGCTGTTCTGGAAGGCGGACGACCAGGCCGTGCGGCTGAACGAGCTGATGACCCTGGACCCGGCCCT
GAAGGATATCCCGCTGCGGCGCGATGTCCGCTCCCTGGGTCGGTTGCTGGGTGAGGTGATCAAGGAGCAGGCGGGGCCGG
CTGCCTACGATGCCGAGGAAGAGCTCCGTCGGCTGGCCATCAGCCATCGGGCACTCGCCGACCAGGAGGAAGCGGTCCTC
GACAGCCCCGCCGAGCAGGAACTCCAGGAACGGGCCGTCAGGATCGTGGGAACGATGACCGTGGCCGAGACCTACCAGAT
CGTCAAGGCCTTCGGGACCTTCTTCGAGCTCACCAACCTGGCGGAGACCAACCACCGGATGCGCCGGCGCCGGGCCACGC
AACTTTCACCCGCTGCCCCCGTGAAGCCCGGTTCCCTGCTGGGCACCCTGATGCGGATGCGGGATGGGGGAATAACGGCC
GAAAAAGCCCTCGATCTGCTGCGGCAGGTGGAGATCATGCCGGTCTTCACCGCTCATCCGACGGAAGTGGCGCGGCGTGT
GGTCCTCTTCAAGCGACGCCGGATCGCCCGGGAGCTGGCGGAGCTTGACCTCCTCCCCCTGGCCGGCGACGAGGCTGACC
GGCGACAGGAGGCCATCTTCGCGGAGATCACCGCCCTCTGGCAGACGGACGAGGTGCGCCGCCGTCCCCAGGGGGTGACC
GACGAGATCAGGATGGGGCTCGACCACTATCCCGGCTCCCTCATGGCGCCCCTGGCGGGCCTCTATGAAGAAATGGCCGA
GGATTTCCGCCAGGTCTACGGCGCGGACCTCCCCGATGCTGCCTTCCCCACCGTGATCCGCTTCGGCTCCTGGATCGGCG
GGGATCGGGACGGCAACCCCAACGTGACAGTCGGCACCACCCGACACGCCCTGGAATCAGCCCGCCAGATGATCCTCGCC
CGGTACCTGGACGGGGTCGAGGAACTGAAGGAGCTCCTGACGCCGTCAACCAATCGGGTGACGGTCTCGCCGCAGCTCCG
GGAGGCCCTGGAGCGCTATGTCGCCGCCCTGGCCGGAGCAGCCGCGGAAACCGGCATCTACCCCGAGTGCGAGCCATACC
GGAAATTCCTCCGGTGTGTCCACCACCGCCTCATGCAGACCCGCGACGGGGAAGACCACCCCGACGCCTATCCCGATGCC
GGAGCCTTCGCCGCCGACCTCATGCTGGTGCGCACAAGCCTCGCCCAGGGGGAAGGGGAGCGGCTGGCACGCCGCTACGT
GGACCCGCTCCTGCGGCAGGTGGAGACCTTCGGCTTCCACCTCCACGCCCTCGATATCCGGCAGCATGCCCGGATCCATG
CCCGGGCCGTGGCAGAGCTGGCCGCCGGCGCCGGCAGCTGCGAGGAGGGGGTTCACCCCGTCCCTCCCCCCCCGTCCGCC
GAGACCACCGAACTCCTCGACACCCTCCGGGCCGTGGCAAAGCTGAAGCAGCGCTACTCCCCCAAGGCAATCAGGAGCTA
CGTCATCAGCGGCGCCTCCTCGGCCCGGGACACCCTCTCCCTCATCTGGCTCATGGAGCTCTGCGGCATCCGCGTGGCCG
CCTCGCCGGACGGAAAGGACCCGGGGGTGATGCCGGTCCCCCTCTTCGAGTCCATCGAAGACCTGCGCAACGCGCCGGGG
ATCTGCCGCGCCCTCTGGTGCTCGCCCGAATACGGGCCGTTTCTCGATTCGTGGGGACGCCGGCAAGAGGTGATGCTCGG
CTATTCGGATTCCAACAAGGACGGGGGGATGTTCACGAGCACCTGGGAGATCCACAAGGCCCACCGGGATCTCCAGCGGG
TGGCCCAGGAGTGCGGAGTGCGTCTGCGCCTCTTTCACGGCCGCGGCGGCACCGTGGGGAGGGGGGGCGGCCCCACCCAC
CGGGCCATCATCGCCCAGCCGGCCGACGGCTTCTCCGGCGCCCTCAAGATCACCGAGCAGGGAGAGGTCATCAACTGGAA
ATACTCCGACCCGTCCCTGGCGAAACGGAACCTGGAGCTGATGGTGGCCGCCTCCCTGGAGACCCTGGCCCGCGCCGGCC
TCGTGGAGACACGGCCCGAGCCGGCATGGGAGGAAGCCCTGGAGGAGATGTCGGGGAGCGCCTTCACCTTCTACCGTGAG
CGGATCACCGGCAACCCCGACATCCTCCCCTACTTCGAGCAGGCCACGCCGGTCCTGGAATTCGAGCTGGCAAAGATCGG
CTCCCGCCCCGCGCGCCGGAGCCGGAGCCGGGATATAGCGGAGCTGCGGGCCATACCCTGGGGCTTCGGCTGGATACAGA
GCCGCCACGTGATACCGGGCTGGTTCGGGGTCGGCTTCGCCCTGGATCGCTTCGCCGTCGGGGGAGAAGGTCGGTTGGAG
TTCCTCAGGTCCATGATGGGGCGCTTCCCCTTCTTTTTCGATCTGATCAGGAACGTGGAACTGGCCCTCACCAAGGTGGA
TCTCCCCCTGGCCCGACTCTACGCGTCCCTGGTGACCGACGCGGGAATTCGCGAGCGGGTCTTCGCCATGATCGTGGAGG
AATACCAACGGACCCGGCGGATGGTCCTGGCGGTCACGGGCCAGGCGCGGCTTCTGGAAAAGAACCCGGCCCTGACCGCG
TCGCTGCGGCTGCGGAACCCCTACATCGACCCCCTGAGCATGATCCAGATCGAGCTCCTGCGGCGCAAGCGGGCCGGGGA
AGAGAGCGACGAGCTGAACTACGTCCTGGCGGCCACCATCAACGGCATTGCCGCGGGGCTAAGGAACACGGGATAA

Upstream 100 bases:

>100_bases
GCAAGCGCCACCCCTTCACGTGGGAATGCCAACCCAGCGGCACGGCATTTGAATCACAGCAGCAACAACAGGGACACAAC
CATCACCGGAGGATGAAATC

Downstream 100 bases:

>100_bases
CAGGGACCGAAGGGAAGGGAGAAAGGGTTACACTGCCCCCTTGGCCTCACCTTCGGCAATCTCCAGGATCTCCCGGTAAA
TGGCGATCATGACCCCTGCC

Product: phosphoenolpyruvate carboxylase

Products: NA

Alternate protein names: PEPC; PEPCase [H]

Number of amino acids: Translated: 931; Mature: 930

Protein sequence:

>931_residues
MAERELFWKADDQAVRLNELMTLDPALKDIPLRRDVRSLGRLLGEVIKEQAGPAAYDAEEELRRLAISHRALADQEEAVL
DSPAEQELQERAVRIVGTMTVAETYQIVKAFGTFFELTNLAETNHRMRRRRATQLSPAAPVKPGSLLGTLMRMRDGGITA
EKALDLLRQVEIMPVFTAHPTEVARRVVLFKRRRIARELAELDLLPLAGDEADRRQEAIFAEITALWQTDEVRRRPQGVT
DEIRMGLDHYPGSLMAPLAGLYEEMAEDFRQVYGADLPDAAFPTVIRFGSWIGGDRDGNPNVTVGTTRHALESARQMILA
RYLDGVEELKELLTPSTNRVTVSPQLREALERYVAALAGAAAETGIYPECEPYRKFLRCVHHRLMQTRDGEDHPDAYPDA
GAFAADLMLVRTSLAQGEGERLARRYVDPLLRQVETFGFHLHALDIRQHARIHARAVAELAAGAGSCEEGVHPVPPPPSA
ETTELLDTLRAVAKLKQRYSPKAIRSYVISGASSARDTLSLIWLMELCGIRVAASPDGKDPGVMPVPLFESIEDLRNAPG
ICRALWCSPEYGPFLDSWGRRQEVMLGYSDSNKDGGMFTSTWEIHKAHRDLQRVAQECGVRLRLFHGRGGTVGRGGGPTH
RAIIAQPADGFSGALKITEQGEVINWKYSDPSLAKRNLELMVAASLETLARAGLVETRPEPAWEEALEEMSGSAFTFYRE
RITGNPDILPYFEQATPVLEFELAKIGSRPARRSRSRDIAELRAIPWGFGWIQSRHVIPGWFGVGFALDRFAVGGEGRLE
FLRSMMGRFPFFFDLIRNVELALTKVDLPLARLYASLVTDAGIRERVFAMIVEEYQRTRRMVLAVTGQARLLEKNPALTA
SLRLRNPYIDPLSMIQIELLRRKRAGEESDELNYVLAATINGIAAGLRNTG

Sequences:

>Translated_931_residues
MAERELFWKADDQAVRLNELMTLDPALKDIPLRRDVRSLGRLLGEVIKEQAGPAAYDAEEELRRLAISHRALADQEEAVL
DSPAEQELQERAVRIVGTMTVAETYQIVKAFGTFFELTNLAETNHRMRRRRATQLSPAAPVKPGSLLGTLMRMRDGGITA
EKALDLLRQVEIMPVFTAHPTEVARRVVLFKRRRIARELAELDLLPLAGDEADRRQEAIFAEITALWQTDEVRRRPQGVT
DEIRMGLDHYPGSLMAPLAGLYEEMAEDFRQVYGADLPDAAFPTVIRFGSWIGGDRDGNPNVTVGTTRHALESARQMILA
RYLDGVEELKELLTPSTNRVTVSPQLREALERYVAALAGAAAETGIYPECEPYRKFLRCVHHRLMQTRDGEDHPDAYPDA
GAFAADLMLVRTSLAQGEGERLARRYVDPLLRQVETFGFHLHALDIRQHARIHARAVAELAAGAGSCEEGVHPVPPPPSA
ETTELLDTLRAVAKLKQRYSPKAIRSYVISGASSARDTLSLIWLMELCGIRVAASPDGKDPGVMPVPLFESIEDLRNAPG
ICRALWCSPEYGPFLDSWGRRQEVMLGYSDSNKDGGMFTSTWEIHKAHRDLQRVAQECGVRLRLFHGRGGTVGRGGGPTH
RAIIAQPADGFSGALKITEQGEVINWKYSDPSLAKRNLELMVAASLETLARAGLVETRPEPAWEEALEEMSGSAFTFYRE
RITGNPDILPYFEQATPVLEFELAKIGSRPARRSRSRDIAELRAIPWGFGWIQSRHVIPGWFGVGFALDRFAVGGEGRLE
FLRSMMGRFPFFFDLIRNVELALTKVDLPLARLYASLVTDAGIRERVFAMIVEEYQRTRRMVLAVTGQARLLEKNPALTA
SLRLRNPYIDPLSMIQIELLRRKRAGEESDELNYVLAATINGIAAGLRNTG
>Mature_930_residues
AERELFWKADDQAVRLNELMTLDPALKDIPLRRDVRSLGRLLGEVIKEQAGPAAYDAEEELRRLAISHRALADQEEAVLD
SPAEQELQERAVRIVGTMTVAETYQIVKAFGTFFELTNLAETNHRMRRRRATQLSPAAPVKPGSLLGTLMRMRDGGITAE
KALDLLRQVEIMPVFTAHPTEVARRVVLFKRRRIARELAELDLLPLAGDEADRRQEAIFAEITALWQTDEVRRRPQGVTD
EIRMGLDHYPGSLMAPLAGLYEEMAEDFRQVYGADLPDAAFPTVIRFGSWIGGDRDGNPNVTVGTTRHALESARQMILAR
YLDGVEELKELLTPSTNRVTVSPQLREALERYVAALAGAAAETGIYPECEPYRKFLRCVHHRLMQTRDGEDHPDAYPDAG
AFAADLMLVRTSLAQGEGERLARRYVDPLLRQVETFGFHLHALDIRQHARIHARAVAELAAGAGSCEEGVHPVPPPPSAE
TTELLDTLRAVAKLKQRYSPKAIRSYVISGASSARDTLSLIWLMELCGIRVAASPDGKDPGVMPVPLFESIEDLRNAPGI
CRALWCSPEYGPFLDSWGRRQEVMLGYSDSNKDGGMFTSTWEIHKAHRDLQRVAQECGVRLRLFHGRGGTVGRGGGPTHR
AIIAQPADGFSGALKITEQGEVINWKYSDPSLAKRNLELMVAASLETLARAGLVETRPEPAWEEALEEMSGSAFTFYRER
ITGNPDILPYFEQATPVLEFELAKIGSRPARRSRSRDIAELRAIPWGFGWIQSRHVIPGWFGVGFALDRFAVGGEGRLEF
LRSMMGRFPFFFDLIRNVELALTKVDLPLARLYASLVTDAGIRERVFAMIVEEYQRTRRMVLAVTGQARLLEKNPALTAS
LRLRNPYIDPLSMIQIELLRRKRAGEESDELNYVLAATINGIAAGLRNTG

Specific function: Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle [H]

COG id: COG2352

COG function: function code C; Phosphoenolpyruvate carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEPCase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790393, Length=944, Percent_Identity=33.0508474576271, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021135
- InterPro:   IPR018129
- InterPro:   IPR022805
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00311 PEPcase [H]

EC number: =4.1.1.31 [H]

Molecular weight: Translated: 103903; Mature: 103772

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00781 PEPCASE_1 ; PS00393 PEPCASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERELFWKADDQAVRLNELMTLDPALKDIPLRRDVRSLGRLLGEVIKEQAGPAAYDAEE
CCCCCCEECCCCCHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ELRRLAISHRALADQEEAVLDSPAEQELQERAVRIVGTMTVAETYQIVKAFGTFFELTNL
HHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AETNHRMRRRRATQLSPAAPVKPGSLLGTLMRMRDGGITAEKALDLLRQVEIMPVFTAHP
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEECCCH
TEVARRVVLFKRRRIARELAELDLLPLAGDEADRRQEAIFAEITALWQTDEVRRRPQGVT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
DEIRMGLDHYPGSLMAPLAGLYEEMAEDFRQVYGADLPDAAFPTVIRFGSWIGGDRDGNP
HHHHHCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCC
NVTVGTTRHALESARQMILARYLDGVEELKELLTPSTNRVTVSPQLREALERYVAALAGA
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHH
AAETGIYPECEPYRKFLRCVHHRLMQTRDGEDHPDAYPDAGAFAADLMLVRTSLAQGEGE
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHH
RLARRYVDPLLRQVETFGFHLHALDIRQHARIHARAVAELAAGAGSCEEGVHPVPPPPSA
HHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
ETTELLDTLRAVAKLKQRYSPKAIRSYVISGASSARDTLSLIWLMELCGIRVAASPDGKD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEECCCCCCC
PGVMPVPLFESIEDLRNAPGICRALWCSPEYGPFLDSWGRRQEVMLGYSDSNKDGGMFTS
CCCCCCHHHHHHHHHHCCCCHHHHEECCCCCCCCHHHCCCCCEEEEEECCCCCCCCEEEC
TWEIHKAHRDLQRVAQECGVRLRLFHGRGGTVGRGGGPTHRAIIAQPADGFSGALKITEQ
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECC
GEVINWKYSDPSLAKRNLELMVAASLETLARAGLVETRPEPAWEEALEEMSGSAFTFYRE
CCEEEEECCCHHHHHCCHHHEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEHHHH
RITGNPDILPYFEQATPVLEFELAKIGSRPARRSRSRDIAELRAIPWGFGWIQSRHVIPG
HHCCCCCCCCCHHHCCCHHHHHHHHHCCCCHHHHHCCCHHHHHCCCCCHHHHCCCCCCCC
WFGVGFALDRFAVGGEGRLEFLRSMMGRFPFFFDLIRNVELALTKVDLPLARLYASLVTD
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHCHHHHHEECCCHHHHHHHHHHHC
AGIRERVFAMIVEEYQRTRRMVLAVTGQARLLEKNPALTASLRLRNPYIDPLSMIQIELL
CCHHHHHHHHHHHHHHHHHHEEEEECCCHHEEECCCCEEEEEEECCCCCCHHHHHHHHHH
RRKRAGEESDELNYVLAATINGIAAGLRNTG
HHHHCCCCCCCCCEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AERELFWKADDQAVRLNELMTLDPALKDIPLRRDVRSLGRLLGEVIKEQAGPAAYDAEE
CCCCCEECCCCCHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ELRRLAISHRALADQEEAVLDSPAEQELQERAVRIVGTMTVAETYQIVKAFGTFFELTNL
HHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AETNHRMRRRRATQLSPAAPVKPGSLLGTLMRMRDGGITAEKALDLLRQVEIMPVFTAHP
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEECCCH
TEVARRVVLFKRRRIARELAELDLLPLAGDEADRRQEAIFAEITALWQTDEVRRRPQGVT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
DEIRMGLDHYPGSLMAPLAGLYEEMAEDFRQVYGADLPDAAFPTVIRFGSWIGGDRDGNP
HHHHHCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCC
NVTVGTTRHALESARQMILARYLDGVEELKELLTPSTNRVTVSPQLREALERYVAALAGA
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHH
AAETGIYPECEPYRKFLRCVHHRLMQTRDGEDHPDAYPDAGAFAADLMLVRTSLAQGEGE
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHH
RLARRYVDPLLRQVETFGFHLHALDIRQHARIHARAVAELAAGAGSCEEGVHPVPPPPSA
HHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
ETTELLDTLRAVAKLKQRYSPKAIRSYVISGASSARDTLSLIWLMELCGIRVAASPDGKD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEECCCCCCC
PGVMPVPLFESIEDLRNAPGICRALWCSPEYGPFLDSWGRRQEVMLGYSDSNKDGGMFTS
CCCCCCHHHHHHHHHHCCCCHHHHEECCCCCCCCHHHCCCCCEEEEEECCCCCCCCEEEC
TWEIHKAHRDLQRVAQECGVRLRLFHGRGGTVGRGGGPTHRAIIAQPADGFSGALKITEQ
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECC
GEVINWKYSDPSLAKRNLELMVAASLETLARAGLVETRPEPAWEEALEEMSGSAFTFYRE
CCEEEEECCCHHHHHCCHHHEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEHHHH
RITGNPDILPYFEQATPVLEFELAKIGSRPARRSRSRDIAELRAIPWGFGWIQSRHVIPG
HHCCCCCCCCCHHHCCCHHHHHHHHHCCCCHHHHHCCCHHHHHCCCCCHHHHCCCCCCCC
WFGVGFALDRFAVGGEGRLEFLRSMMGRFPFFFDLIRNVELALTKVDLPLARLYASLVTD
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHCHHHHHEECCCHHHHHHHHHHHC
AGIRERVFAMIVEEYQRTRRMVLAVTGQARLLEKNPALTASLRLRNPYIDPLSMIQIELL
CCHHHHHHHHHHHHHHHHHHEEEEECCCHHEEECCCCEEEEEEECCCCCCHHHHHHHHHH
RRKRAGEESDELNYVLAATINGIAAGLRNTG
HHHHCCCCCCCCCEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]