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The map label for this gene is mspIM [H]

Identifier: 78221478

GI number: 78221478

Start: 285104

End: 286099

Strand: Direct

Name: mspIM [H]

Synonym: Gmet_0255

Alternate gene names: 78221478

Gene position: 285104-286099 (Clockwise)

Preceding gene: 78221476

Following gene: 78221479

Centisome position: 7.13

GC content: 67.17

Gene sequence:

>996_bases
TTGATGCAGGTCAAGGAAGGAGAGGGGCGTTGGGGGAATGGGGGACCGACAGTGACTGAGGTGAAAACAGCCGCGCCACT
GCGGACGCTGGAGCTTTTCTGCGGGATCGGCGGGTTTTCCGCCGCAGTGGAGGGGGGAAACGTGCGGATCGTGGGGGCCT
TCGACCAGGATCCGGCGGCCCTGGACACCTATCGGCTCAACTTCCCCGGCCATGGGGCAAGGAAGGTGGACCTGGAACGG
GTAAGCGCCTGGGAGCTGACGGCCGGTGGCGTTGATCTCTGGTGGCTCTCTCCCCCCTGCCAGCCTTACTGCGAGCGGGG
GGCGCGTCGGGATCTGGCCGATCCCCGCGCCCGGAGCCTCGTCCATATCCTGGAACTGCTGGAGCGGATTCCCGACGATC
TCCTCCCCCGCCATCTGGCCCTGGAGAACGTGGCCGGCTTTGTGGGCTCCGAGGGCCACGGCCTGCTCACCGAAGTCCTT
ACTTCCCGGGGCTTCCAGGTGCGGGAGCGGTTTCTCTGCCCCACGGAACTGGGAGCCCCCATGCGGCGGCCCCGCTACTA
CCTGGCCGCCTCCCGCGACGAGATGCGACCCCTCGTAGCCCCGGAACCTCGTCCTCTCCGCCCGCTGGCCGGGTACCTCG
ACCGGCGGTTTGACGGCGAGGTGCCCACTGAGCTGCTCCTTTCTCCCGACGTCGTTGCCCGTTTCGGCGGAGCCCTTCCC
ATCCTTGACTCTGGCGACGGTTCAGCCTGTGCCACCTGCTTCACCGCCGGGTATGGGAGATCCATCACGTCCGCCGGCTC
CTACCTGCAATGCGCAAGCGGCGTGCGCCACTTTTCTCCGGAGGAGATCGTGCGGTTCATGGCGTTTCCGGAGGGATTCC
GCTTCCCCGACGAAGTCCCGCTCAGGAAGCGGTGGCACCTGATCGGCAACAGCCTCTCGGTGGCTGCGGTGCGCGAGGTG
CTCCGGGTGTTCCCCTCTCTGGGATTGTCGCCGTGA

Upstream 100 bases:

>100_bases
GATGATTGCGGTTACGATTATCTCGCTGGAAGTCATGGTGTTCCTCCCGGTCCGTGGGTGTGCGTTCGGTGAATGGTAGC
ACCGGGGGGCATGGGCGGCA

Downstream 100 bases:

>100_bases
ATCGATGATGAAAATCCGTCCAACCATCCCGAAAACATGATATAACCCGGCAGGTTGTTATTTATTTTCGGCCTTCTGCC
CGGCCCCAGGAGCCCCCATG

Product: C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.MspI; Cytosine-specific methyltransferase MspI [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MMQVKEGEGRWGNGGPTVTEVKTAAPLRTLELFCGIGGFSAAVEGGNVRIVGAFDQDPAALDTYRLNFPGHGARKVDLER
VSAWELTAGGVDLWWLSPPCQPYCERGARRDLADPRARSLVHILELLERIPDDLLPRHLALENVAGFVGSEGHGLLTEVL
TSRGFQVRERFLCPTELGAPMRRPRYYLAASRDEMRPLVAPEPRPLRPLAGYLDRRFDGEVPTELLLSPDVVARFGGALP
ILDSGDGSACATCFTAGYGRSITSAGSYLQCASGVRHFSPEEIVRFMAFPEGFRFPDEVPLRKRWHLIGNSLSVAAVREV
LRVFPSLGLSP

Sequences:

>Translated_331_residues
MMQVKEGEGRWGNGGPTVTEVKTAAPLRTLELFCGIGGFSAAVEGGNVRIVGAFDQDPAALDTYRLNFPGHGARKVDLER
VSAWELTAGGVDLWWLSPPCQPYCERGARRDLADPRARSLVHILELLERIPDDLLPRHLALENVAGFVGSEGHGLLTEVL
TSRGFQVRERFLCPTELGAPMRRPRYYLAASRDEMRPLVAPEPRPLRPLAGYLDRRFDGEVPTELLLSPDVVARFGGALP
ILDSGDGSACATCFTAGYGRSITSAGSYLQCASGVRHFSPEEIVRFMAFPEGFRFPDEVPLRKRWHLIGNSLSVAAVREV
LRVFPSLGLSP
>Mature_331_residues
MMQVKEGEGRWGNGGPTVTEVKTAAPLRTLELFCGIGGFSAAVEGGNVRIVGAFDQDPAALDTYRLNFPGHGARKVDLER
VSAWELTAGGVDLWWLSPPCQPYCERGARRDLADPRARSLVHILELLERIPDDLLPRHLALENVAGFVGSEGHGLLTEVL
TSRGFQVRERFLCPTELGAPMRRPRYYLAASRDEMRPLVAPEPRPLRPLAGYLDRRFDGEVPTELLLSPDVVARFGGALP
ILDSGDGSACATCFTAGYGRSITSAGSYLQCASGVRHFSPEEIVRFMAFPEGFRFPDEVPLRKRWHLIGNSLSVAAVREV
LRVFPSLGLSP

Specific function: This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MspI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4758184, Length=179, Percent_Identity=37.4301675977654, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI116007318, Length=350, Percent_Identity=28, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI17137742, Length=326, Percent_Identity=26.9938650306748, Blast_Score=125, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 36252; Mature: 36252

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQVKEGEGRWGNGGPTVTEVKTAAPLRTLELFCGIGGFSAAVEGGNVRIVGAFDQDPAA
CCCCCCCCCCCCCCCCCCEEEHHHCCHHHHHHHHCCCCHHHEECCCCEEEEEECCCCCCC
LDTYRLNFPGHGARKVDLERVSAWELTAGGVDLWWLSPPCQPYCERGARRDLADPRARSL
CCEEEECCCCCCCCEEHHHHHCEEEEECCCEEEEEECCCCHHHHHCCCCCCCCCHHHHHH
VHILELLERIPDDLLPRHLALENVAGFVGSEGHGLLTEVLTSRGFQVRERFLCPTELGAP
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHCCCCHHHCCC
MRRPRYYLAASRDEMRPLVAPEPRPLRPLAGYLDRRFDGEVPTELLLSPDVVARFGGALP
CCCCCEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHCCCCCC
ILDSGDGSACATCFTAGYGRSITSAGSYLQCASGVRHFSPEEIVRFMAFPEGFRFPDEVP
EEECCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
LRKRWHLIGNSLSVAAVREVLRVFPSLGLSP
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMQVKEGEGRWGNGGPTVTEVKTAAPLRTLELFCGIGGFSAAVEGGNVRIVGAFDQDPAA
CCCCCCCCCCCCCCCCCCEEEHHHCCHHHHHHHHCCCCHHHEECCCCEEEEEECCCCCCC
LDTYRLNFPGHGARKVDLERVSAWELTAGGVDLWWLSPPCQPYCERGARRDLADPRARSL
CCEEEECCCCCCCCEEHHHHHCEEEEECCCEEEEEECCCCHHHHHCCCCCCCCCHHHHHH
VHILELLERIPDDLLPRHLALENVAGFVGSEGHGLLTEVLTSRGFQVRERFLCPTELGAP
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHCCCCHHHCCC
MRRPRYYLAASRDEMRPLVAPEPRPLRPLAGYLDRRFDGEVPTELLLSPDVVARFGGALP
CCCCCEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHCCCCCC
ILDSGDGSACATCFTAGYGRSITSAGSYLQCASGVRHFSPEEIVRFMAFPEGFRFPDEVP
EEECCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
LRKRWHLIGNSLSVAAVREVLRVFPSLGLSP
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2471145 [H]