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The map label for this gene is alkK [H]

Identifier: 78221479

GI number: 78221479

Start: 286197

End: 287843

Strand: Direct

Name: alkK [H]

Synonym: Gmet_0256

Alternate gene names: 78221479

Gene position: 286197-287843 (Clockwise)

Preceding gene: 78221478

Following gene: 78221480

Centisome position: 7.16

GC content: 66.18

Gene sequence:

>1647_bases
ATGTCCGACCCCCTCATTCCCCGTACCCCCTCGGCCTACGACTATCCGCTGCTGATCAAGAACCTCCTCTTCTGCCCCGT
GGTGGACAACCCGGACCAGGAGATCGTCTACCGCGATCTCTACCGCGGCACCTACCGCGGCCTTCGGGAGCGGGTCCGGC
GCCTGGCCAGCGTCCTGACCGGGCTGGGGGTGAAGCCCGGCGACACCGTGGCGGTCATGGACTGGGACAGCCACCGCTAC
CTGGAACTCTTCTTTGCCGTGCCGATGATCGGTGCGGTGCTCCATACCATCAACGTCCGACTCTCGCCGGAGCAGATCCT
CTACACCATCGACCATGCGGAGGACGACCTCCTCCTGGTGAACAGTGAATTCCTCCCGATCCTGGAGCAGATCCGGGGAC
GGCTCGACGCGGTGCAAGGGTACGTGCTCCTTACCGACGAGGCGAAGGCGCCCGAAACCGCCATCCCCTTCCTCGGGGAG
TACGAGGCGCTCCTGGCGGCAGCAGCGCCGGAATTCGAGTTCCCCGACTTCGACGAGAACACCCGGGCCACCACCTTCTA
CACCACCGGCACCACCGGCATGCCCAAGGGGGTCTACTTCAGCCACCGCCAGCTGGTCCTCCACTCCTTCGGCGTCATGA
CGGCCTTGAGCACGGCGGTGGGGCACGGCACGTTCCGCCGCACCGACGTCTACATGCCCATAACCCCCATGTTCCACGTT
CACGCCTGGGGGATGCCTTACGTGGCCTCCATGCTGGGGGTGAAACAGGTCTATCCGGGGCGCTACGTGCCGGACCAGCT
CCTGGAGCTCATCGAGCGGGAGAAGGTGACCTTTTCCCACTGCGTCCCCACCATCCTCCACATGCTCCTGAAGCATCCCC
ATGCCGAGCGTATCGATCTCTCGGGGTGGAAGGTGATCATCGGCGGCGCGGCCATGTCCCGGACTCTCTGCCTGGAGGCG
CTGCGGCGCGGCATCGATGTCTTCACCGGCTACGGCATGTCCGAGACCTGCCCCATCCTCTCCCTTTCCCATCTCACGCC
GGAGATGCTGGAACTTTCCCCGGAGGAGCAGGCGACGATCCGCTGCAAGACCGGCCAGGCCCTGCCGCTGGTGGATCTGC
GGGTGGCGGGGGGCGAGCTGCGGGAGCTTCCCCGGGACGGGGTGAGCAGCGGCGAGATCGTGGTTCGGGCCCCCTGGCTC
ACCCAGGGGTACCTGAAGGACCACAAGGCCTCGGAGCGGCTCTGGGACGGAGGATATCTCCACACCGGCGACGTGGCGGT
CCGGGATGGGTTGGGATACGTGCGGATCACCGACCGGACCAAGGATGTCATCAAGGTGGCGGGGGAGTGGGTCTCGTCCC
TGGAACTGGAGGACATCTTCGCCCACCATCCGGCCGTGGCCGAGGTGGCGGTCATCGGCCAGCCCGACGAGAAATGGGGG
GAGCGCCCCCTGGCCCTGGTGGTCGCGAAGCCCGGCGAAGCCGGCCGGGTGACGGAGAAGGAACTGGTCCACCATGTGCG
GGAGTACGCCGACAAAGGGGTCGTCAGCAAGCAGGTGGTCCTTGCCCGAGTCCGCCTCGTGGAGGCCATCGACAAGACCA
GCGTGGGCAAGACCAACAAGGTCGCCCTGCGGGAAAAGTACCTGTAG

Upstream 100 bases:

>100_bases
TGAATCGATGATGAAAATCCGTCCAACCATCCCGAAAACATGATATAACCCGGCAGGTTGTTATTTATTTTCGGCCTTCT
GCCCGGCCCCAGGAGCCCCC

Downstream 100 bases:

>100_bases
CCCCCAAGGCTGCGGGATGGAGAGCCAATGAGCAATACCAGCCGTATCCGCCGCCAGCGCTGGGTGATCTTCTCGGTGCT
GGCGCTCATGTATATTCTCG

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSDPLIPRTPSAYDYPLLIKNLLFCPVVDNPDQEIVYRDLYRGTYRGLRERVRRLASVLTGLGVKPGDTVAVMDWDSHRY
LELFFAVPMIGAVLHTINVRLSPEQILYTIDHAEDDLLLVNSEFLPILEQIRGRLDAVQGYVLLTDEAKAPETAIPFLGE
YEALLAAAAPEFEFPDFDENTRATTFYTTGTTGMPKGVYFSHRQLVLHSFGVMTALSTAVGHGTFRRTDVYMPITPMFHV
HAWGMPYVASMLGVKQVYPGRYVPDQLLELIEREKVTFSHCVPTILHMLLKHPHAERIDLSGWKVIIGGAAMSRTLCLEA
LRRGIDVFTGYGMSETCPILSLSHLTPEMLELSPEEQATIRCKTGQALPLVDLRVAGGELRELPRDGVSSGEIVVRAPWL
TQGYLKDHKASERLWDGGYLHTGDVAVRDGLGYVRITDRTKDVIKVAGEWVSSLELEDIFAHHPAVAEVAVIGQPDEKWG
ERPLALVVAKPGEAGRVTEKELVHHVREYADKGVVSKQVVLARVRLVEAIDKTSVGKTNKVALREKYL

Sequences:

>Translated_548_residues
MSDPLIPRTPSAYDYPLLIKNLLFCPVVDNPDQEIVYRDLYRGTYRGLRERVRRLASVLTGLGVKPGDTVAVMDWDSHRY
LELFFAVPMIGAVLHTINVRLSPEQILYTIDHAEDDLLLVNSEFLPILEQIRGRLDAVQGYVLLTDEAKAPETAIPFLGE
YEALLAAAAPEFEFPDFDENTRATTFYTTGTTGMPKGVYFSHRQLVLHSFGVMTALSTAVGHGTFRRTDVYMPITPMFHV
HAWGMPYVASMLGVKQVYPGRYVPDQLLELIEREKVTFSHCVPTILHMLLKHPHAERIDLSGWKVIIGGAAMSRTLCLEA
LRRGIDVFTGYGMSETCPILSLSHLTPEMLELSPEEQATIRCKTGQALPLVDLRVAGGELRELPRDGVSSGEIVVRAPWL
TQGYLKDHKASERLWDGGYLHTGDVAVRDGLGYVRITDRTKDVIKVAGEWVSSLELEDIFAHHPAVAEVAVIGQPDEKWG
ERPLALVVAKPGEAGRVTEKELVHHVREYADKGVVSKQVVLARVRLVEAIDKTSVGKTNKVALREKYL
>Mature_547_residues
SDPLIPRTPSAYDYPLLIKNLLFCPVVDNPDQEIVYRDLYRGTYRGLRERVRRLASVLTGLGVKPGDTVAVMDWDSHRYL
ELFFAVPMIGAVLHTINVRLSPEQILYTIDHAEDDLLLVNSEFLPILEQIRGRLDAVQGYVLLTDEAKAPETAIPFLGEY
EALLAAAAPEFEFPDFDENTRATTFYTTGTTGMPKGVYFSHRQLVLHSFGVMTALSTAVGHGTFRRTDVYMPITPMFHVH
AWGMPYVASMLGVKQVYPGRYVPDQLLELIEREKVTFSHCVPTILHMLLKHPHAERIDLSGWKVIIGGAAMSRTLCLEAL
RRGIDVFTGYGMSETCPILSLSHLTPEMLELSPEEQATIRCKTGQALPLVDLRVAGGELRELPRDGVSSGEIVVRAPWLT
QGYLKDHKASERLWDGGYLHTGDVAVRDGLGYVRITDRTKDVIKVAGEWVSSLELEDIFAHHPAVAEVAVIGQPDEKWGE
RPLALVVAKPGEAGRVTEKELVHHVREYADKGVVSKQVVLARVRLVEAIDKTSVGKTNKVALREKYL

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=549, Percent_Identity=24.9544626593807, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI187761345, Length=535, Percent_Identity=27.2897196261682, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI187761343, Length=535, Percent_Identity=27.2897196261682, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI42544132, Length=529, Percent_Identity=21.3610586011342, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI38505220, Length=519, Percent_Identity=23.5067437379576, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI28416953, Length=559, Percent_Identity=26.2969588550984, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI115511026, Length=526, Percent_Identity=23.0038022813688, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI58082049, Length=523, Percent_Identity=21.6061185468451, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI157311624, Length=526, Percent_Identity=21.6730038022814, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157311622, Length=526, Percent_Identity=21.6730038022814, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1788107, Length=529, Percent_Identity=26.0869565217391, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI221142682, Length=488, Percent_Identity=25.6147540983607, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI145693145, Length=374, Percent_Identity=26.4705882352941, Blast_Score=129, Evalue=7e-31,
Organism=Escherichia coli, GI1786810, Length=526, Percent_Identity=24.3346007604563, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790505, Length=535, Percent_Identity=24.2990654205607, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788595, Length=300, Percent_Identity=24.6666666666667, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786529, Length=537, Percent_Identity=26.2569832402235, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559526, Length=532, Percent_Identity=26.8796992481203, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17531443, Length=521, Percent_Identity=24.5681381957774, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17557194, Length=523, Percent_Identity=24.6653919694073, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=517, Percent_Identity=23.9845261121857, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=521, Percent_Identity=25.3358925143954, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=507, Percent_Identity=25.2465483234714, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=507, Percent_Identity=25.2465483234714, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=507, Percent_Identity=25.2465483234714, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=538, Percent_Identity=24.3494423791822, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=503, Percent_Identity=24.4532803180915, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=569, Percent_Identity=21.9683655536028, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=515, Percent_Identity=25.2427184466019, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=518, Percent_Identity=24.9034749034749, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17556552, Length=511, Percent_Identity=24.6575342465753, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115534440, Length=529, Percent_Identity=23.4404536862004, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=383, Percent_Identity=24.2819843342037, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21355181, Length=506, Percent_Identity=25.296442687747, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI18859661, Length=377, Percent_Identity=28.1167108753316, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922652, Length=503, Percent_Identity=23.8568588469185, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24581924, Length=544, Percent_Identity=25.5514705882353, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648260, Length=554, Percent_Identity=23.6462093862816, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24653035, Length=565, Percent_Identity=21.4159292035398, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20130357, Length=565, Percent_Identity=21.7699115044248, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648257, Length=554, Percent_Identity=24.187725631769, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24656500, Length=515, Percent_Identity=22.9126213592233, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356441, Length=508, Percent_Identity=20.6692913385827, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583465, Length=469, Percent_Identity=22.3880597014925, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24583463, Length=469, Percent_Identity=22.3880597014925, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281364798, Length=469, Percent_Identity=22.3880597014925, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355225, Length=560, Percent_Identity=24.1071428571429, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356947, Length=367, Percent_Identity=25.3405994550409, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358303, Length=366, Percent_Identity=24.0437158469945, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 61125; Mature: 60994

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPLIPRTPSAYDYPLLIKNLLFCPVVDNPDQEIVYRDLYRGTYRGLRERVRRLASVLT
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGVKPGDTVAVMDWDSHRYLELFFAVPMIGAVLHTINVRLSPEQILYTIDHAEDDLLLV
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCEEECHHHEEEEEECCCCCEEEE
NSEFLPILEQIRGRLDAVQGYVLLTDEAKAPETAIPFLGEYEALLAAAAPEFEFPDFDEN
CCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
TRATTFYTTGTTGMPKGVYFSHRQLVLHSFGVMTALSTAVGHGTFRRTDVYMPITPMFHV
CCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEE
HAWGMPYVASMLGVKQVYPGRYVPDQLLELIEREKVTFSHCVPTILHMLLKHPHAERIDL
EECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEC
SGWKVIIGGAAMSRTLCLEALRRGIDVFTGYGMSETCPILSLSHLTPEMLELSPEEQATI
CCCEEEEECHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCEEHHHCCHHHHHCCCCCCCEE
RCKTGQALPLVDLRVAGGELRELPRDGVSSGEIVVRAPWLTQGYLKDHKASERLWDGGYL
EEECCCCCCEEEEEECCCHHHHCCCCCCCCCCEEEECCCCCCHHHHHCCHHHHHCCCCCE
HTGDVAVRDGLGYVRITDRTKDVIKVAGEWVSSLELEDIFAHHPAVAEVAVIGQPDEKWG
EECCEEEECCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCHHHC
ERPLALVVAKPGEAGRVTEKELVHHVREYADKGVVSKQVVLARVRLVEAIDKTSVGKTNK
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
VALREKYL
CHHHHCCC
>Mature Secondary Structure 
SDPLIPRTPSAYDYPLLIKNLLFCPVVDNPDQEIVYRDLYRGTYRGLRERVRRLASVLT
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGVKPGDTVAVMDWDSHRYLELFFAVPMIGAVLHTINVRLSPEQILYTIDHAEDDLLLV
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCEEECHHHEEEEEECCCCCEEEE
NSEFLPILEQIRGRLDAVQGYVLLTDEAKAPETAIPFLGEYEALLAAAAPEFEFPDFDEN
CCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
TRATTFYTTGTTGMPKGVYFSHRQLVLHSFGVMTALSTAVGHGTFRRTDVYMPITPMFHV
CCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEE
HAWGMPYVASMLGVKQVYPGRYVPDQLLELIEREKVTFSHCVPTILHMLLKHPHAERIDL
EECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEC
SGWKVIIGGAAMSRTLCLEALRRGIDVFTGYGMSETCPILSLSHLTPEMLELSPEEQATI
CCCEEEEECHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCEEHHHCCHHHHHCCCCCCCEE
RCKTGQALPLVDLRVAGGELRELPRDGVSSGEIVVRAPWLTQGYLKDHKASERLWDGGYL
EEECCCCCCEEEEEECCCHHHHCCCCCCCCCCEEEECCCCCCHHHHHCCHHHHHCCCCCE
HTGDVAVRDGLGYVRITDRTKDVIKVAGEWVSSLELEDIFAHHPAVAEVAVIGQPDEKWG
EECCEEEECCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCHHHC
ERPLALVVAKPGEAGRVTEKELVHHVREYADKGVVSKQVVLARVRLVEAIDKTSVGKTNK
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
VALREKYL
CHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]