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The map label for this gene is folD1

Identifier: 78221470

GI number: 78221470

Start: 277815

End: 278687

Strand: Direct

Name: folD1

Synonym: Gmet_0247

Alternate gene names: 78221470

Gene position: 277815-278687 (Clockwise)

Preceding gene: 78221469

Following gene: 78221471

Centisome position: 6.95

GC content: 63.0

Gene sequence:

>873_bases
ATGAAATTACTGGACGGGAAGAAGTGTGCCGAGAGCCTGGTTGCCGACATCGCCAGGAAAGTGGCAGGGTATGAAGAGTC
GGGCCTCCGGAAGCCCCACATGACGGTCATTCTCGTGGGGGAGCACGCACCGAGCGAGTCCTACGTGAAGTCCAAGATCA
AGTCGTGCGGGAATGCCGGGTTCGAGGGGACGCTCCTGCGCTTCCCGGACACCATCACCGAGGCGGAGCTTCTGGAGAAG
ATCCGCGGGATCAACGCCGACCCCACCACCGACGGCCTCATCGTGCAGCTCCCCCTCCCCCGGCACATCAACCAGCAGCA
CATCATCAACGCCATTGCCCCCGAGAAGGACATCGACGGGTTCCACCCCACGAACTTCGGCCGCATGACCCTGGGGCAGA
AGGCGTTCCGCCCCGCCACCGCCTACGGCATCTGCAAGCTGCTGCAGTTCTACGAGATCCCGGTCTGGGGGAAGCACTGC
GTGGTCATCGGCCGCTCCAACATCGTGGGAAAGCCCATCTCCATCATGCTCTCCAACGACTTCGACATCGGCAACGCCAC
CGTGACCCTGACCCACATCGAGACCCCCCGGGAGCTCCTGTTGGACGAAACGCGCCGGGCCGACATCGTCATCGTGGCCG
TCGGCATCCCCGGCTTCGTCACCGAGGACATGGTGAAGGAGGGGGTGGTTGTCATCGACGTGGGGATCAACCGGCTGGAG
GACGGCAAGATTGTGGGGGACGTGGACTTCGAGAACGTGAAGAAGAAGTGCTCCTGGATCACCCCGGTCCCCGGCGGCGT
GGGGCGCATGACGGTGGCCGCCCTCATGATCAACACCCTCATGGCCTACCAGAACAATTTCGACCTGGTCTGA

Upstream 100 bases:

>100_bases
TGTCGCCGGTTGTGACATCCCTGATTCTGTGTTACAAAAGAGATGAGCCTTGAACCTCCCGATTTGTATGCGGAAAATAG
ATAAAGGAGCATGAGTTGCC

Downstream 100 bases:

>100_bases
GGATATCCGGCGCCCGCCGGAACCCTAACGTGACATCCCCCGTGAACGGCGGCCCCTGGCCGCCGTTTTCATTTTTGTGC
GGCGACTGTTGCCGGGCAAG

Product: methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKLLDGKKCAESLVADIARKVAGYEESGLRKPHMTVILVGEHAPSESYVKSKIKSCGNAGFEGTLLRFPDTITEAELLEK
IRGINADPTTDGLIVQLPLPRHINQQHIINAIAPEKDIDGFHPTNFGRMTLGQKAFRPATAYGICKLLQFYEIPVWGKHC
VVIGRSNIVGKPISIMLSNDFDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTEDMVKEGVVVIDVGINRLE
DGKIVGDVDFENVKKKCSWITPVPGGVGRMTVAALMINTLMAYQNNFDLV

Sequences:

>Translated_290_residues
MKLLDGKKCAESLVADIARKVAGYEESGLRKPHMTVILVGEHAPSESYVKSKIKSCGNAGFEGTLLRFPDTITEAELLEK
IRGINADPTTDGLIVQLPLPRHINQQHIINAIAPEKDIDGFHPTNFGRMTLGQKAFRPATAYGICKLLQFYEIPVWGKHC
VVIGRSNIVGKPISIMLSNDFDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTEDMVKEGVVVIDVGINRLE
DGKIVGDVDFENVKKKCSWITPVPGGVGRMTVAALMINTLMAYQNNFDLV
>Mature_290_residues
MKLLDGKKCAESLVADIARKVAGYEESGLRKPHMTVILVGEHAPSESYVKSKIKSCGNAGFEGTLLRFPDTITEAELLEK
IRGINADPTTDGLIVQLPLPRHINQQHIINAIAPEKDIDGFHPTNFGRMTLGQKAFRPATAYGICKLLQFYEIPVWGKHC
VVIGRSNIVGKPISIMLSNDFDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTEDMVKEGVVVIDVGINRLE
DGKIVGDVDFENVKKKCSWITPVPGGVGRMTVAALMINTLMAYQNNFDLV

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=46.9594594594595, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=46.2328767123288, Blast_Score=255, Evalue=3e-68,
Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=39.041095890411, Blast_Score=188, Evalue=5e-48,
Organism=Escherichia coli, GI1786741, Length=281, Percent_Identity=49.4661921708185, Blast_Score=258, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17568735, Length=293, Percent_Identity=38.9078498293515, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6319558, Length=299, Percent_Identity=44.1471571906354, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6321643, Length=305, Percent_Identity=39.672131147541, Blast_Score=191, Evalue=9e-50,
Organism=Saccharomyces cerevisiae, GI6322933, Length=322, Percent_Identity=25.1552795031056, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=45.6081081081081, Blast_Score=258, Evalue=3e-69,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=45.6081081081081, Blast_Score=258, Evalue=3e-69,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=38.4083044982699, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=38.4083044982699, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=38.4083044982699, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=38.4083044982699, Blast_Score=178, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD1_GEOMG (Q39Z32)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383217.1
- ProteinModelPortal:   Q39Z32
- SMR:   Q39Z32
- STRING:   Q39Z32
- GeneID:   3739710
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0247
- NMPDR:   fig|269799.3.peg.277
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   GFHPTNF
- PhylomeDB:   Q39Z32
- ProtClustDB:   CLSK743076
- BioCyc:   GMET269799:GMET_0247-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31744; Mature: 31744

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLDGKKCAESLVADIARKVAGYEESGLRKPHMTVILVGEHAPSESYVKSKIKSCGNAG
CCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCC
FEGTLLRFPDTITEAELLEKIRGINADPTTDGLIVQLPLPRHINQQHIINAIAPEKDIDG
CCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCC
FHPTNFGRMTLGQKAFRPATAYGICKLLQFYEIPVWGKHCVVIGRSNIVGKPISIMLSND
CCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECC
FDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTEDMVKEGVVVIDVGINRLE
CCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCEEEEEECCCCCC
DGKIVGDVDFENVKKKCSWITPVPGGVGRMTVAALMINTLMAYQNNFDLV
CCCEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLLDGKKCAESLVADIARKVAGYEESGLRKPHMTVILVGEHAPSESYVKSKIKSCGNAG
CCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCC
FEGTLLRFPDTITEAELLEKIRGINADPTTDGLIVQLPLPRHINQQHIINAIAPEKDIDG
CCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCC
FHPTNFGRMTLGQKAFRPATAYGICKLLQFYEIPVWGKHCVVIGRSNIVGKPISIMLSND
CCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECC
FDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTEDMVKEGVVVIDVGINRLE
CCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCEEEEEECCCCCC
DGKIVGDVDFENVKKKCSWITPVPGGVGRMTVAALMINTLMAYQNNFDLV
CCCEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA