Click here to switch to the map view.

The map label for this gene is argG

Identifier: 78221430

GI number: 78221430

Start: 239389

End: 240609

Strand: Direct

Name: argG

Synonym: Gmet_0206

Alternate gene names: 78221430

Gene position: 239389-240609 (Clockwise)

Preceding gene: 78221429

Following gene: 78221431

Centisome position: 5.99

GC content: 61.92

Gene sequence:

>1221_bases
ATGGCCAAAGTGCACAAAGACGTGAAAAAGATCGTCCTCGCCTACTCGGGCGGGCTCGACACCTCCATCATCCTCAAGTG
GCTCAAGAACGAGTACGGCTGCGAGGTCATAGCCTTCTCCGCCGACCTGGGCCAGGGCGACGAGCTTGCCCCGATCCGCG
ACAAAGCCTTCGCTACCGGCGCCGACAAGGTCTATATCGACGATCTGAAGGAAGAGTTCGTCAGGGACTTCGTATTCCCC
ATGTTCCGCGCCAACGCCATCTACGAGGGTCACTACCTCCTGGGCACCTCCATCGCCCGGCCACTCATCGCCAAGCGCCA
GATGGAGATCGCGAAGATCGAGGGAGCCGACGCCGTATCCCATGGCGCCACCGGCAAGGGGAACGATCAGGTCCGTTTCG
AGCTGGCCTACTACCACTTTGACCCGGCCATCACGGTTGTGGTCCCGTGGCGGGAGTGGAAGCTCAACAGCCGCCAGGCC
CTGGTGAACTACGCCAAGAAAAACGGGATTCCGATCCCGGTCACCAAGAAGCGTCCCTGGTCCTCGGACCGCAACATGCT
TCACATCTCCTTCGAAGGGGGTATCCTGGAGGATACCTGGGCCGAGCCCCCCGAGAACATGTACGTGCTCACCAAGGCGC
CGGAGAAGGCCCCCAACAAGCCCCAGTTCGTGGAGATCGAGTTCAAGAACGGCAACGCCGTGGCCGTTGACGGCGAGAAG
ATGAGCCCGGCCCAGCTTCTGGCCCACCTGAACTACATCGGCGGCGAGCACGGCATCGGCCGGGTGGACCTGCTGGAGAA
CCGTTCCGTGGGGATGAAGTCACGGGGCGTGTACGAGACCCCCGGCGGCACCATCCTGCGCGAGGCCCACTCGGCGGTGG
AGCAGATCACCATGGACCGCGAAGTCATGAGAATCCGCGACTCCCTTATCCCCGAGTACGCCCGCCAGGTCTATTCCGGC
TACTGGTTCTCCCCGGAGCGGGAAATGCTCCAGACCCTGATTGACGACTCCCAGAAGTGCGTGAACGGCGTGGCCCGGGT
GAAGCTCTACAAGGGGCATTGCCGCACCGTAGGCCGCAAGTCCGAGACCAACTCCCTCTTCAACCTGGATTTCGCCACCT
TCGAGAAGGATCAGGTATTCAACCAGGCCGACGCCACCGGCTTCATCAAGATCAACTCCCTGCGGCTGCGGATCAGGGCG
CTCATGCAGGGGAAAAAGTAG

Upstream 100 bases:

>100_bases
CCGTCTGGGACGAGGCAGAGAACCGGCTCCACGTACAGAAAGCCATCATGGCTTCGTTGTTGAAGTAACTGATCCCATTC
CGATCCAAAAGGAGACTTAC

Downstream 100 bases:

>100_bases
GAAAACGCGCCTTTTCGCAATCTCTGAATCATGACATCAGGGACACGGTTCGCCGTGTCCCTTCGTCTATCCCATGACCA
AGAAGACTTTCAAGAAAGAC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MAKVHKDVKKIVLAYSGGLDTSIILKWLKNEYGCEVIAFSADLGQGDELAPIRDKAFATGADKVYIDDLKEEFVRDFVFP
MFRANAIYEGHYLLGTSIARPLIAKRQMEIAKIEGADAVSHGATGKGNDQVRFELAYYHFDPAITVVVPWREWKLNSRQA
LVNYAKKNGIPIPVTKKRPWSSDRNMLHISFEGGILEDTWAEPPENMYVLTKAPEKAPNKPQFVEIEFKNGNAVAVDGEK
MSPAQLLAHLNYIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHSAVEQITMDREVMRIRDSLIPEYARQVYSG
YWFSPEREMLQTLIDDSQKCVNGVARVKLYKGHCRTVGRKSETNSLFNLDFATFEKDQVFNQADATGFIKINSLRLRIRA
LMQGKK

Sequences:

>Translated_406_residues
MAKVHKDVKKIVLAYSGGLDTSIILKWLKNEYGCEVIAFSADLGQGDELAPIRDKAFATGADKVYIDDLKEEFVRDFVFP
MFRANAIYEGHYLLGTSIARPLIAKRQMEIAKIEGADAVSHGATGKGNDQVRFELAYYHFDPAITVVVPWREWKLNSRQA
LVNYAKKNGIPIPVTKKRPWSSDRNMLHISFEGGILEDTWAEPPENMYVLTKAPEKAPNKPQFVEIEFKNGNAVAVDGEK
MSPAQLLAHLNYIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHSAVEQITMDREVMRIRDSLIPEYARQVYSG
YWFSPEREMLQTLIDDSQKCVNGVARVKLYKGHCRTVGRKSETNSLFNLDFATFEKDQVFNQADATGFIKINSLRLRIRA
LMQGKK
>Mature_405_residues
AKVHKDVKKIVLAYSGGLDTSIILKWLKNEYGCEVIAFSADLGQGDELAPIRDKAFATGADKVYIDDLKEEFVRDFVFPM
FRANAIYEGHYLLGTSIARPLIAKRQMEIAKIEGADAVSHGATGKGNDQVRFELAYYHFDPAITVVVPWREWKLNSRQAL
VNYAKKNGIPIPVTKKRPWSSDRNMLHISFEGGILEDTWAEPPENMYVLTKAPEKAPNKPQFVEIEFKNGNAVAVDGEKM
SPAQLLAHLNYIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHSAVEQITMDREVMRIRDSLIPEYARQVYSGY
WFSPEREMLQTLIDDSQKCVNGVARVKLYKGHCRTVGRKSETNSLFNLDFATFEKDQVFNQADATGFIKINSLRLRIRAL
MQGKK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=404, Percent_Identity=50, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI16950633, Length=404, Percent_Identity=50, Blast_Score=383, Evalue=1e-106,
Organism=Escherichia coli, GI1789563, Length=404, Percent_Identity=25.7425742574257, Blast_Score=119, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324514, Length=402, Percent_Identity=42.7860696517413, Blast_Score=351, Evalue=1e-97,
Organism=Drosophila melanogaster, GI21358151, Length=401, Percent_Identity=43.640897755611, Blast_Score=338, Evalue=3e-93,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_GEOMG (Q39Z72)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383177.1
- HSSP:   Q9X2A1
- ProteinModelPortal:   Q39Z72
- SMR:   Q39Z72
- STRING:   Q39Z72
- GeneID:   3739668
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0206
- NMPDR:   fig|269799.3.peg.237
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- PhylomeDB:   Q39Z72
- ProtClustDB:   PRK00509
- BioCyc:   GMET269799:GMET_0206-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45766; Mature: 45635

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 41-41 BINDING 92-92 BINDING 97-97 BINDING 122-122 BINDING 124-124 BINDING 128-128 BINDING 128-128 BINDING 129-129 BINDING 132-132 BINDING 181-181 BINDING 190-190 BINDING 266-266 BINDING 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVHKDVKKIVLAYSGGLDTSIILKWLKNEYGCEVIAFSADLGQGDELAPIRDKAFATG
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHCCC
ADKVYIDDLKEEFVRDFVFPMFRANAIYEGHYLLGTSIARPLIAKRQMEIAKIEGADAVS
CCEEEHHHHHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHEEECCCCHHC
HGATGKGNDQVRFELAYYHFDPAITVVVPWREWKLNSRQALVNYAKKNGIPIPVTKKRPW
CCCCCCCCCEEEEEEEEEEECCEEEEEECCHHCCCCCHHHHHHHHHHCCCCCCCCCCCCC
SSDRNMLHISFEGGILEDTWAEPPENMYVLTKAPEKAPNKPQFVEIEFKNGNAVAVDGEK
CCCCCEEEEEECCCEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEECCCC
MSPAQLLAHLNYIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHSAVEQITMDR
CCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHH
EVMRIRDSLIPEYARQVYSGYWFSPEREMLQTLIDDSQKCVNGVARVKLYKGHCRTVGRK
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHEEEEECHHHHCCCC
SETNSLFNLDFATFEKDQVFNQADATGFIKINSLRLRIRALMQGKK
CCCCCEEEEEHHHCCHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKVHKDVKKIVLAYSGGLDTSIILKWLKNEYGCEVIAFSADLGQGDELAPIRDKAFATG
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHCCC
ADKVYIDDLKEEFVRDFVFPMFRANAIYEGHYLLGTSIARPLIAKRQMEIAKIEGADAVS
CCEEEHHHHHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHEEECCCCHHC
HGATGKGNDQVRFELAYYHFDPAITVVVPWREWKLNSRQALVNYAKKNGIPIPVTKKRPW
CCCCCCCCCEEEEEEEEEEECCEEEEEECCHHCCCCCHHHHHHHHHHCCCCCCCCCCCCC
SSDRNMLHISFEGGILEDTWAEPPENMYVLTKAPEKAPNKPQFVEIEFKNGNAVAVDGEK
CCCCCEEEEEECCCEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEECCCC
MSPAQLLAHLNYIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHSAVEQITMDR
CCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHH
EVMRIRDSLIPEYARQVYSGYWFSPEREMLQTLIDDSQKCVNGVARVKLYKGHCRTVGRK
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHEEEEECHHHHCCCC
SETNSLFNLDFATFEKDQVFNQADATGFIKINSLRLRIRALMQGKK
CCCCCEEEEEHHHCCHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA