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The map label for this gene is argF

Identifier: 78221429

GI number: 78221429

Start: 238445

End: 239356

Strand: Direct

Name: argF

Synonym: Gmet_0205

Alternate gene names: 78221429

Gene position: 238445-239356 (Clockwise)

Preceding gene: 78221428

Following gene: 78221430

Centisome position: 5.96

GC content: 60.64

Gene sequence:

>912_bases
ATGACCCGGCATTTTCTTGCCCTCAACCAGTATACCAAAGAAGCCCTCGACGCCCTCTTCGCCCTGACACGGGAGCTGAA
GGAAGAGCAGAAAAAGGGGATTCCCCACCGGATACTGGAGGGAAAAAGCGTCGCCCTCATTTTCGAAAAGTCGTCCACCA
GGACTCGTATTTCCTTTGAGGTGGGGGTTCATCAGCTGGGAGCCCATCCGCTTTTCATCTCCTCGGCCACGTCCCAGATG
GGGCGGGGCGAGCCCGTAAGGGACACCGCCCGCGTCATGGCCCGCTACTGTGACGGCGTCATGATCCGCACTTACGGCCA
GGAGATTGTGGAGGAGTTTGCCCGCTATTCGTCGGTGCCGGTCATAAACGGCCTCACCGACCTGTTCCATCCCTGCCAGA
TCATGGCCGATCTCTTTACGGTCATCGAGCACAAGGGGAAATACGACGGGCTGAAATTCGCCTGGGTCGGCGACGGCAAC
AACATGGCCAATACCTGGATCGAGGCCGCCGCCATCCTCGGCTTCGACCTGGCTCTGGCCTGCCCGGAGGGGTATGAGCC
TGACCGCACTGTCTGGGACTGGGCCCAGAAGAAAGCCACGTCGTCCATTACTATCACCCGGGATCCAAAAGAGGCCGTGC
GCGACGCCGATGTCGTCAACACCGACGTCTGGGCCAGCATGGGGCAGGAGCAGGAACAGAAGGTGCGCGAGGTGGCGTTC
AAGGGCTACTGTCTCGACGACGACCTGGTTGCCCTGGCTAAGCCCGACTGCATGGTTCTCCACTGCCTCCCGGCCCACCG
GGGTGAGGAGATCACCGACAGCGTCATCGAAGGCCCCCGTTCCGCCGTCTGGGACGAGGCAGAGAACCGGCTCCACGTAC
AGAAAGCCATCATGGCTTCGTTGTTGAAGTAA

Upstream 100 bases:

>100_bases
AGGACAAGGTGCTCCGGTTCGTGCCGCCGCTCATCGTGACCAAGCAGGAAGTGAATGAAATGATCGCCGTGCTTGACGGC
ATTCTGGAGGAGATTAAGCA

Downstream 100 bases:

>100_bases
CTGATCCCATTCCGATCCAAAAGGAGACTTACATGGCCAAAGTGCACAAAGACGTGAAAAAGATCGTCCTCGCCTACTCG
GGCGGGCTCGACACCTCCAT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTRHFLALNQYTKEALDALFALTRELKEEQKKGIPHRILEGKSVALIFEKSSTRTRISFEVGVHQLGAHPLFISSATSQM
GRGEPVRDTARVMARYCDGVMIRTYGQEIVEEFARYSSVPVINGLTDLFHPCQIMADLFTVIEHKGKYDGLKFAWVGDGN
NMANTWIEAAAILGFDLALACPEGYEPDRTVWDWAQKKATSSITITRDPKEAVRDADVVNTDVWASMGQEQEQKVREVAF
KGYCLDDDLVALAKPDCMVLHCLPAHRGEEITDSVIEGPRSAVWDEAENRLHVQKAIMASLLK

Sequences:

>Translated_303_residues
MTRHFLALNQYTKEALDALFALTRELKEEQKKGIPHRILEGKSVALIFEKSSTRTRISFEVGVHQLGAHPLFISSATSQM
GRGEPVRDTARVMARYCDGVMIRTYGQEIVEEFARYSSVPVINGLTDLFHPCQIMADLFTVIEHKGKYDGLKFAWVGDGN
NMANTWIEAAAILGFDLALACPEGYEPDRTVWDWAQKKATSSITITRDPKEAVRDADVVNTDVWASMGQEQEQKVREVAF
KGYCLDDDLVALAKPDCMVLHCLPAHRGEEITDSVIEGPRSAVWDEAENRLHVQKAIMASLLK
>Mature_302_residues
TRHFLALNQYTKEALDALFALTRELKEEQKKGIPHRILEGKSVALIFEKSSTRTRISFEVGVHQLGAHPLFISSATSQMG
RGEPVRDTARVMARYCDGVMIRTYGQEIVEEFARYSSVPVINGLTDLFHPCQIMADLFTVIEHKGKYDGLKFAWVGDGNN
MANTWIEAAAILGFDLALACPEGYEPDRTVWDWAQKKATSSITITRDPKEAVRDADVVNTDVWASMGQEQEQKVREVAFK
GYCLDDDLVALAKPDCMVLHCLPAHRGEEITDSVIEGPRSAVWDEAENRLHVQKAIMASLLK

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=304, Percent_Identity=42.4342105263158, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI18105007, Length=307, Percent_Identity=29.6416938110749, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=41.5902140672783, Blast_Score=240, Evalue=9e-65,
Organism=Escherichia coli, GI1786469, Length=326, Percent_Identity=40.7975460122699, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI2367364, Length=315, Percent_Identity=27.3015873015873, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI48994908, Length=359, Percent_Identity=24.5125348189415, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193204318, Length=330, Percent_Identity=27.5757575757576, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322373, Length=308, Percent_Identity=35.0649350649351, Blast_Score=192, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=27.0967741935484, Blast_Score=99, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=27.7955271565495, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_GEOMG (Q39Z73)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383176.1
- HSSP:   P96108
- ProteinModelPortal:   Q39Z73
- SMR:   Q39Z73
- STRING:   Q39Z73
- GeneID:   3739667
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0205
- NMPDR:   fig|269799.3.peg.236
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   FTVAMYQ
- PhylomeDB:   Q39Z73
- ProtClustDB:   PRK00779
- BioCyc:   GMET269799:GMET_0205-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33943; Mature: 33812

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 103-103 BINDING 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRHFLALNQYTKEALDALFALTRELKEEQKKGIPHRILEGKSVALIFEKSSTRTRISFE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEEECCCCCEEEEEE
VGVHQLGAHPLFISSATSQMGRGEPVRDTARVMARYCDGVMIRTYGQEIVEEFARYSSVP
HHHHHHCCCCEEEECHHHHHCCCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCC
VINGLTDLFHPCQIMADLFTVIEHKGKYDGLKFAWVGDGNNMANTWIEAAAILGFDLALA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEE
CPEGYEPDRTVWDWAQKKATSSITITRDPKEAVRDADVVNTDVWASMGQEQEQKVREVAF
CCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHH
KGYCLDDDLVALAKPDCMVLHCLPAHRGEEITDSVIEGPRSAVWDEAENRLHVQKAIMAS
CCCCCCCCCHHHCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LLK
HCC
>Mature Secondary Structure 
TRHFLALNQYTKEALDALFALTRELKEEQKKGIPHRILEGKSVALIFEKSSTRTRISFE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEEECCCCCEEEEEE
VGVHQLGAHPLFISSATSQMGRGEPVRDTARVMARYCDGVMIRTYGQEIVEEFARYSSVP
HHHHHHCCCCEEEECHHHHHCCCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCC
VINGLTDLFHPCQIMADLFTVIEHKGKYDGLKFAWVGDGNNMANTWIEAAAILGFDLALA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEE
CPEGYEPDRTVWDWAQKKATSSITITRDPKEAVRDADVVNTDVWASMGQEQEQKVREVAF
CCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHH
KGYCLDDDLVALAKPDCMVLHCLPAHRGEEITDSVIEGPRSAVWDEAENRLHVQKAIMAS
CCCCCCCCCHHHCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LLK
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA