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The map label for this gene is merA [H]

Identifier: 78221401

GI number: 78221401

Start: 205113

End: 206522

Strand: Reverse

Name: merA [H]

Synonym: Gmet_0177

Alternate gene names: 78221401

Gene position: 206522-205113 (Counterclockwise)

Preceding gene: 78221407

Following gene: 78221400

Centisome position: 5.17

GC content: 66.95

Gene sequence:

>1410_bases
ATGCCCGACGACACACACGATCTCGTCATCCTCGGCTCCGGCTCCACCGCCTTTGCCGCGGCGCTCAGGGCCCAGTCCTA
CGGGACGCGGGTCCTCATGGTGGAAAAGGGGGTGCCGGGCGGCACCTGCATCAACTGGGGGTGCGTCCCAAGCAAGACGC
TGATCCATGCCGCGTTATTCTATCAGGAGGGGAAGCTCGGGGCAAGGCTCGGGCTCGGGGAATGCGGCGGGACGGTGGTT
CTAGAGCGCCTCATGGCCCGCAAAGATCAGGTGGTGGGGCATCTCCGCCAGACGAAATACCTTGATATCCTCCAGGATGT
GCCGGGGCTTCAACTGGTGAAGGGGACCGGCCGCTTCCTAGGGCCCGACCGGCTGGAGGTGGGGGACCGGGAGATCAGGA
GCGAGCGGTTTCTGGTGGCCGTGGGGGGGGATCCCCGGGTCCCGCGGATTCCTGGTTTGGAATCGACCCCCTTTCTCACG
AGCCGGGGGACTCTCCTCCTCAAGACCATTCCTCAGTCCCTTGTCATCATCGGCGGCGGGGTCATCGCCGTGGAAATGGG
GCAGATGTTCCAGCGCCTCGGCGCCAAGGTAACGATCCTGGAGCACGGTCCCCGCATCCTGGGGCCGGTGGAGCCGGAGC
CGGCCCTGGCGGTGCGTGACTTTCTGCGGGCGGAGGGAATGAAGATCGTCTGCCGCACCACGATCTGCTTGGCGGCCCAG
GACGGGGCCGGGGTGCGCGTGGAGGCGGAACGGGACGGGGAGCGGGTGAGCTTCACCGCCGAAAAGCTCCTCGTGGCGAC
GGGAACGGCTCCGGCCACCAACGGGATCGGGCTGGAGCTGGCCGGGGTTGAAACCGACCCGCGGGGGTTCGTGACGGTGG
ATGAACGGATGCGGACCACGGCGCCGGGTATCTGGGCCGCCGGCGACTGCACCGGCGGGATGATGATCGCCACGGTCGGC
GCCCGGGAAGGGATCGTGGCGGTGGACGACATGCTGAACCCCGGCTGCGGCTGCTCCATGGATTTTTTGAGCGCTCCCAT
GGCCATCTTCACCGACCCGGAGGTGGGGATGGTGGGGCACACCGAAGAGGGTGCCAAAGCCGCCGGCTTCGACGTGGTCG
TGAATGTGATGCCGGTGGCGGCCATCCCCAAGGCCCACGTCACCGGCCACACAGCCGGGGTCATCAAGATGGTGGCGGAC
AAGGCCACGGGCCGTCTCCTGGGGGTGCATCTGGCCTGCCACCGGGGAGCGGACATCATCAACGAGGCAGCCCTGGCCAT
CCGCTTCCGGGCCACGGTGGAGGATCTGGCAAACGCGTTGCATGTCTATCCTTCGATGGGGGAGGGCCTGAGGCTCTGCG
CCCAGGGATTCAGTAGGGATATCAGCCGGTTGTCGTGCTGTGCCGAATAG

Upstream 100 bases:

>100_bases
TAAGTACTGGTGCTGACATGGTGTCCTCCCTGACAATTGTTGTTCAAAGCTTCTCAAACGGCTGCGGTCGGGTACACTTT
CTCTCAAAGGAGATCCGACA

Downstream 100 bases:

>100_bases
CTGTTCCACACGGATCATGACGGGGCGGGCCATGGAGAGGAGCGCAAAAAGTACACACCTGGACCCTGAATTGTCACGGC
GGCTCCACCGGGCCCTCACC

Product: mercuric reductase MerA

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MPDDTHDLVILGSGSTAFAAALRAQSYGTRVLMVEKGVPGGTCINWGCVPSKTLIHAALFYQEGKLGARLGLGECGGTVV
LERLMARKDQVVGHLRQTKYLDILQDVPGLQLVKGTGRFLGPDRLEVGDREIRSERFLVAVGGDPRVPRIPGLESTPFLT
SRGTLLLKTIPQSLVIIGGGVIAVEMGQMFQRLGAKVTILEHGPRILGPVEPEPALAVRDFLRAEGMKIVCRTTICLAAQ
DGAGVRVEAERDGERVSFTAEKLLVATGTAPATNGIGLELAGVETDPRGFVTVDERMRTTAPGIWAAGDCTGGMMIATVG
AREGIVAVDDMLNPGCGCSMDFLSAPMAIFTDPEVGMVGHTEEGAKAAGFDVVVNVMPVAAIPKAHVTGHTAGVIKMVAD
KATGRLLGVHLACHRGADIINEAALAIRFRATVEDLANALHVYPSMGEGLRLCAQGFSRDISRLSCCAE

Sequences:

>Translated_469_residues
MPDDTHDLVILGSGSTAFAAALRAQSYGTRVLMVEKGVPGGTCINWGCVPSKTLIHAALFYQEGKLGARLGLGECGGTVV
LERLMARKDQVVGHLRQTKYLDILQDVPGLQLVKGTGRFLGPDRLEVGDREIRSERFLVAVGGDPRVPRIPGLESTPFLT
SRGTLLLKTIPQSLVIIGGGVIAVEMGQMFQRLGAKVTILEHGPRILGPVEPEPALAVRDFLRAEGMKIVCRTTICLAAQ
DGAGVRVEAERDGERVSFTAEKLLVATGTAPATNGIGLELAGVETDPRGFVTVDERMRTTAPGIWAAGDCTGGMMIATVG
AREGIVAVDDMLNPGCGCSMDFLSAPMAIFTDPEVGMVGHTEEGAKAAGFDVVVNVMPVAAIPKAHVTGHTAGVIKMVAD
KATGRLLGVHLACHRGADIINEAALAIRFRATVEDLANALHVYPSMGEGLRLCAQGFSRDISRLSCCAE
>Mature_468_residues
PDDTHDLVILGSGSTAFAAALRAQSYGTRVLMVEKGVPGGTCINWGCVPSKTLIHAALFYQEGKLGARLGLGECGGTVVL
ERLMARKDQVVGHLRQTKYLDILQDVPGLQLVKGTGRFLGPDRLEVGDREIRSERFLVAVGGDPRVPRIPGLESTPFLTS
RGTLLLKTIPQSLVIIGGGVIAVEMGQMFQRLGAKVTILEHGPRILGPVEPEPALAVRDFLRAEGMKIVCRTTICLAAQD
GAGVRVEAERDGERVSFTAEKLLVATGTAPATNGIGLELAGVETDPRGFVTVDERMRTTAPGIWAAGDCTGGMMIATVGA
REGIVAVDDMLNPGCGCSMDFLSAPMAIFTDPEVGMVGHTEEGAKAAGFDVVVNVMPVAAIPKAHVTGHTAGVIKMVADK
ATGRLLGVHLACHRGADIINEAALAIRFRATVEDLANALHVYPSMGEGLRLCAQGFSRDISRLSCCAE

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=472, Percent_Identity=33.2627118644068, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=25.9259259259259, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI148277065, Length=468, Percent_Identity=23.7179487179487, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI33519430, Length=468, Percent_Identity=23.7179487179487, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI33519428, Length=468, Percent_Identity=23.7179487179487, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI33519426, Length=468, Percent_Identity=23.7179487179487, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI148277071, Length=467, Percent_Identity=23.7687366167024, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI22035672, Length=469, Percent_Identity=26.865671641791, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI291045266, Length=464, Percent_Identity=21.7672413793103, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=32.967032967033, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786307, Length=473, Percent_Identity=31.2896405919662, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1789915, Length=431, Percent_Identity=28.7703016241299, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87082354, Length=470, Percent_Identity=25.7446808510638, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=32.5327510917031, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI71983429, Length=358, Percent_Identity=27.3743016759777, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=358, Percent_Identity=27.3743016759777, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17557007, Length=478, Percent_Identity=23.4309623430962, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=485, Percent_Identity=23.2989690721649, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6321091, Length=469, Percent_Identity=33.4754797441365, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6325240, Length=478, Percent_Identity=26.1506276150628, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325166, Length=461, Percent_Identity=24.9457700650759, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21358499, Length=464, Percent_Identity=33.4051724137931, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24640549, Length=495, Percent_Identity=26.0606060606061, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24640551, Length=495, Percent_Identity=26.0606060606061, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24640553, Length=495, Percent_Identity=26.0606060606061, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI17737741, Length=501, Percent_Identity=25.9481037924152, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 49393; Mature: 49262

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDDTHDLVILGSGSTAFAAALRAQSYGTRVLMVEKGVPGGTCINWGCVPSKTLIHAALF
CCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHH
YQEGKLGARLGLGECGGTVVLERLMARKDQVVGHLRQTKYLDILQDVPGLQLVKGTGRFL
HCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
GPDRLEVGDREIRSERFLVAVGGDPRVPRIPGLESTPFLTSRGTLLLKTIPQSLVIIGGG
CCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCEEEEECCCEEEEECCC
VIAVEMGQMFQRLGAKVTILEHGPRILGPVEPEPALAVRDFLRAEGMKIVCRTTICLAAQ
EEEEHHHHHHHHHCCEEEEEECCCEEECCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEEC
DGAGVRVEAERDGERVSFTAEKLLVATGTAPATNGIGLELAGVETDPRGFVTVDERMRTT
CCCCEEEEECCCCCEEEEEEEEEEEEECCCCCCCCCCEEEECCCCCCCCEEEECCHHHHC
APGIWAAGDCTGGMMIATVGAREGIVAVDDMLNPGCGCSMDFLSAPMAIFTDPEVGMVGH
CCCEEECCCCCCCEEEEEECCCCCEEEEHHHCCCCCCCCHHHHHCCEEEEECCCCCEECC
TEEGAKAAGFDVVVNVMPVAAIPKAHVTGHTAGVIKMVADKATGRLLGVHLACHRGADII
CCCCCCCCCCEEEEEECCHHCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCHHH
NEAALAIRFRATVEDLANALHVYPSMGEGLRLCAQGFSRDISRLSCCAE
HHHEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDDTHDLVILGSGSTAFAAALRAQSYGTRVLMVEKGVPGGTCINWGCVPSKTLIHAALF
CCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHH
YQEGKLGARLGLGECGGTVVLERLMARKDQVVGHLRQTKYLDILQDVPGLQLVKGTGRFL
HCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
GPDRLEVGDREIRSERFLVAVGGDPRVPRIPGLESTPFLTSRGTLLLKTIPQSLVIIGGG
CCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCEEEEECCCEEEEECCC
VIAVEMGQMFQRLGAKVTILEHGPRILGPVEPEPALAVRDFLRAEGMKIVCRTTICLAAQ
EEEEHHHHHHHHHCCEEEEEECCCEEECCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEEC
DGAGVRVEAERDGERVSFTAEKLLVATGTAPATNGIGLELAGVETDPRGFVTVDERMRTT
CCCCEEEEECCCCCEEEEEEEEEEEEECCCCCCCCCCEEEECCCCCCCCEEEECCHHHHC
APGIWAAGDCTGGMMIATVGAREGIVAVDDMLNPGCGCSMDFLSAPMAIFTDPEVGMVGH
CCCEEECCCCCCCEEEEEECCCCCEEEEHHHCCCCCCCCHHHHHCCEEEEECCCCCEECC
TEEGAKAAGFDVVVNVMPVAAIPKAHVTGHTAGVIKMVADKATGRLLGVHLACHRGADII
CCCCCCCCCCEEEEEECCHHCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCHHH
NEAALAIRFRATVEDLANALHVYPSMGEGLRLCAQGFSRDISRLSCCAE
HHHEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]