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The map label for this gene is nuoN

Identifier: 78221397

GI number: 78221397

Start: 201482

End: 202882

Strand: Direct

Name: nuoN

Synonym: Gmet_0172

Alternate gene names: 78221397

Gene position: 201482-202882 (Clockwise)

Preceding gene: 78221396

Following gene: 78221398

Centisome position: 5.04

GC content: 68.09

Gene sequence:

>1401_bases
ATGACCATAACCGAACTCTGGGCCCTCATCCCTCTTCTGATCCTCGCCTGCGGGAGCGTCTTCGTGCTCATGCTCGGGGC
AATCGTGCCGGGACGGTGCGGAACCGTCATCGGCGTTGCCGTCTGCGCCGGCACGGCTCTCTGGGCGATGCTGGCTCCGC
CGCAGCCCCTGGCGCCGCCGACTCTGGGAGTGACCGTCACCCCCTTTACCCGCTTCTTCCTCGTATTTTTCGCCGTGACG
GCAGGCTTGTCGCTCCTCCTGGCCCGAGACCATGCGGCGCGGCAGGGGCTTAAGGGGGAGGAGTACCCGGCCACGATCCT
CTTCGGCACCTTCGGCATGGGTGTGGTGGCGAGCGCGGCCAACTTCCTGACCCTCTTCCTGGGGCTGGAGGCCCTCACCT
TCGCCTTCTACATTCTGGTGGCCTACGACCACAACCGCCCGGCGTCCGGGGAGGCAGGGCTCAAGTACCTCCTCATGGGG
GCCGTGTCGGCGGCCTTTGTGGCATTCGGCATCGCGCTCCTCTACGGGGCCACGGGGACCCTGGAGATTAGCCGGGCCGT
GGCCGCCTCGGCAGCCGGGGGAGGGATCGCCCTGGCGGGATGGGGGCTCCTCTTGGCGGGGCTCGCCTTCAAGATATCCC
TGGCACCGGCCCACCTCTGGACCCCGGACATCTACCAGGGGGGGCCGACGCCGGTGGTGGCCTTCCTCGCCTCCGGCTCC
AAGGGGGCGGCCATCGCCCTTTTCCTGCTCATTCTCTCCCCCCTCGGAACGCTCGGCACCCTGCGGGCGCCCCTCTGGGG
GCTGGCGTTCCTCTCCATGACCGTCGGGAACCTGGCGGCACTCCTGCAGCCCAACGTGAAGCGGATGCTCGCCTACTCAT
CCGTGGCCCAGATGGGGTACGTGGCCCTGGCGCTCCTGTCGGGGGGACGGGGGTACGAGGCTGCGGCGTTTTATGCCGTG
GCCTACGGAGCCATGGTCCTGGCTGCCTTCGGCGCCCTTGCCTCCCTGGAGGGAGAAACGCCGCTGGAACAGGTGGATGA
CCTCCGGGGGCTCGGCTATCACCGCCCCTTCCAGGGGGTGGTGCTGGCCGTGGCCATGCTCGCCCTGGCAGGGATTCCTC
CAACGGTGGGCTTTGTGGGAAAGTTCGCCATCTTCTTCGCGGCCCTGAAAGGGGGAGAGGCTCCCCTGGCGGTCATCGGC
ATCCTCACGGCGGCGGCCTCGGCCTACTACTACCTGCGGGTGGTGGTGAACCTCTACATGAAACCGGCGGATGAGACGGG
CTCCGGCCAACGCCCCACCGTTGCGGAAGCGCTCTCCCTTGGCATTGCAGCCCTCGCCATCTTCATCCTCGGCATCTGGC
CCGGCCCCCTCTTCGACCTGGCAGCCGCGATTCTTCGCTAG

Upstream 100 bases:

>100_bases
TGGCGGTGCTGGCGACGATCGACGTGTATATCGGTGTCCATCCGAAACCGCTCCTGGATATCCTCAAGGTGCCGGTGGCG
CTCCTGACGGGGGCGACGCC

Downstream 100 bases:

>100_bases
AACGGAGCCCCCGTGCAGAGCATCATAAAGGCCCTCATCAGCCTCGTCATCATCATCGCCTGCGCCAAAATCGGCAAACG
CTTCCCGACCCTCGGGGGCC

Product: proton-translocating NADH-quinone oxidoreductase, chain N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTITELWALIPLLILACGSVFVLMLGAIVPGRCGTVIGVAVCAGTALWAMLAPPQPLAPPTLGVTVTPFTRFFLVFFAVT
AGLSLLLARDHAARQGLKGEEYPATILFGTFGMGVVASAANFLTLFLGLEALTFAFYILVAYDHNRPASGEAGLKYLLMG
AVSAAFVAFGIALLYGATGTLEISRAVAASAAGGGIALAGWGLLLAGLAFKISLAPAHLWTPDIYQGGPTPVVAFLASGS
KGAAIALFLLILSPLGTLGTLRAPLWGLAFLSMTVGNLAALLQPNVKRMLAYSSVAQMGYVALALLSGGRGYEAAAFYAV
AYGAMVLAAFGALASLEGETPLEQVDDLRGLGYHRPFQGVVLAVAMLALAGIPPTVGFVGKFAIFFAALKGGEAPLAVIG
ILTAAASAYYYLRVVVNLYMKPADETGSGQRPTVAEALSLGIAALAIFILGIWPGPLFDLAAAILR

Sequences:

>Translated_466_residues
MTITELWALIPLLILACGSVFVLMLGAIVPGRCGTVIGVAVCAGTALWAMLAPPQPLAPPTLGVTVTPFTRFFLVFFAVT
AGLSLLLARDHAARQGLKGEEYPATILFGTFGMGVVASAANFLTLFLGLEALTFAFYILVAYDHNRPASGEAGLKYLLMG
AVSAAFVAFGIALLYGATGTLEISRAVAASAAGGGIALAGWGLLLAGLAFKISLAPAHLWTPDIYQGGPTPVVAFLASGS
KGAAIALFLLILSPLGTLGTLRAPLWGLAFLSMTVGNLAALLQPNVKRMLAYSSVAQMGYVALALLSGGRGYEAAAFYAV
AYGAMVLAAFGALASLEGETPLEQVDDLRGLGYHRPFQGVVLAVAMLALAGIPPTVGFVGKFAIFFAALKGGEAPLAVIG
ILTAAASAYYYLRVVVNLYMKPADETGSGQRPTVAEALSLGIAALAIFILGIWPGPLFDLAAAILR
>Mature_465_residues
TITELWALIPLLILACGSVFVLMLGAIVPGRCGTVIGVAVCAGTALWAMLAPPQPLAPPTLGVTVTPFTRFFLVFFAVTA
GLSLLLARDHAARQGLKGEEYPATILFGTFGMGVVASAANFLTLFLGLEALTFAFYILVAYDHNRPASGEAGLKYLLMGA
VSAAFVAFGIALLYGATGTLEISRAVAASAAGGGIALAGWGLLLAGLAFKISLAPAHLWTPDIYQGGPTPVVAFLASGSK
GAAIALFLLILSPLGTLGTLRAPLWGLAFLSMTVGNLAALLQPNVKRMLAYSSVAQMGYVALALLSGGRGYEAAAFYAVA
YGAMVLAAFGALASLEGETPLEQVDDLRGLGYHRPFQGVVLAVAMLALAGIPPTVGFVGKFAIFFAALKGGEAPLAVIGI
LTAAASAYYYLRVVVNLYMKPADETGSGQRPTVAEALSLGIAALAIFILGIWPGPLFDLAAAILR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Escherichia coli, GI145693160, Length=377, Percent_Identity=36.0742705570292, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1788614, Length=338, Percent_Identity=28.9940828402367, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788831, Length=293, Percent_Identity=29.0102389078498, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1788829, Length=268, Percent_Identity=27.6119402985075, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_GEOMG (Q39ZA5)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383144.1
- STRING:   Q39ZA5
- GeneID:   3739653
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0172
- NMPDR:   fig|269799.3.peg.204
- HOGENOM:   HBG747830
- OMA:   FAFYILV
- PhylomeDB:   Q39ZA5
- ProtClustDB:   CLSK829274
- BioCyc:   GMET269799:GMET_0172-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 47872; Mature: 47741

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x94b4e78)-; HASH(0x1032da8c)-; HASH(0x1032d978)-; HASH(0x1032b6a8)-; HASH(0x957e2f0)-; HASH(0xf456720)-; HASH(0xfbfcb40)-; HASH(0x1032d9f0)-; HASH(0xfdf82c4)-; HASH(0xfe51cbc)-; HASH(0xfebbe50)-; HASH(0xfbfcda4)-; HASH(0x10169fc8)-; HASH(0x1032d96c)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITELWALIPLLILACGSVFVLMLGAIVPGRCGTVIGVAVCAGTALWAMLAPPQPLAPP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
TLGVTVTPFTRFFLVFFAVTAGLSLLLARDHAARQGLKGEEYPATILFGTFGMGVVASAA
CCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHH
NFLTLFLGLEALTFAFYILVAYDHNRPASGEAGLKYLLMGAVSAAFVAFGIALLYGATGT
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LEISRAVAASAAGGGIALAGWGLLLAGLAFKISLAPAHLWTPDIYQGGPTPVVAFLASGS
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCEEHEEECCC
KGAAIALFLLILSPLGTLGTLRAPLWGLAFLSMTVGNLAALLQPNVKRMLAYSSVAQMGY
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VALALLSGGRGYEAAAFYAVAYGAMVLAAFGALASLEGETPLEQVDDLRGLGYHRPFQGV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHH
VLAVAMLALAGIPPTVGFVGKFAIFFAALKGGEAPLAVIGILTAAASAYYYLRVVVNLYM
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KPADETGSGQRPTVAEALSLGIAALAIFILGIWPGPLFDLAAAILR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
>Mature Secondary Structure 
TITELWALIPLLILACGSVFVLMLGAIVPGRCGTVIGVAVCAGTALWAMLAPPQPLAPP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
TLGVTVTPFTRFFLVFFAVTAGLSLLLARDHAARQGLKGEEYPATILFGTFGMGVVASAA
CCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHH
NFLTLFLGLEALTFAFYILVAYDHNRPASGEAGLKYLLMGAVSAAFVAFGIALLYGATGT
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LEISRAVAASAAGGGIALAGWGLLLAGLAFKISLAPAHLWTPDIYQGGPTPVVAFLASGS
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCEEHEEECCC
KGAAIALFLLILSPLGTLGTLRAPLWGLAFLSMTVGNLAALLQPNVKRMLAYSSVAQMGY
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VALALLSGGRGYEAAAFYAVAYGAMVLAAFGALASLEGETPLEQVDDLRGLGYHRPFQGV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHH
VLAVAMLALAGIPPTVGFVGKFAIFFAALKGGEAPLAVIGILTAAASAYYYLRVVVNLYM
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KPADETGSGQRPTVAEALSLGIAALAIFILGIWPGPLFDLAAAILR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA