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The map label for this gene is nuoM [H]

Identifier: 78221396

GI number: 78221396

Start: 199995

End: 201485

Strand: Direct

Name: nuoM [H]

Synonym: Gmet_0171

Alternate gene names: 78221396

Gene position: 199995-201485 (Clockwise)

Preceding gene: 78221395

Following gene: 78221397

Centisome position: 5.0

GC content: 66.06

Gene sequence:

>1491_bases
GTGACCGACTATCCGATCCTCACCATCCTCATCTTCCTCCCCCTGGCGGGATGCCTCTGTTTAGCGCCGGTCTGGCACTG
CCGGACGTCTGCCCGCGCCGTGGCCCTGGGGTTTGCCGTTGCCGAACTGGCCCTGGCGGCGTGGCTTCTGGTGGCCGCCA
CGGGGATGCCCGCCGCCCCCGGCGCGCCGACGGGGTATTTCCTCTGGGAGGATGCCGTGTGGATCGAGCGCTTCGGCATC
CGCTACCTCCTTGGGATGGACGGGATCAGCCTCCTCATGGTGGCGCTCACGGCCTTCATCACGGTGGTCGCCATGGCGGT
ATCGTGGCGCGCCGTCACCGAGCGGACGGCCCTCCACTATTTCCTCATCCTCCTCATGGAGAGCGGGCTCATGGGGGTCT
TCCTCGCCCTGGATCTCGTCCTCTTCTACCTCTTCTGGGAGGTGATGCTGATCCCCATGTTCTTCCTGATCGGCATCTGG
GGACACGGCCGCCGCATCTACTCGGCGGTGAAGTTCTTCCTCTACACCCTGGCCGGTTCACTCCTGATGCTCCTGGCCAT
CATCGGGGTCTACCTCATACATGGCGATGCCACCGGCATCTACACCTTCACCCTCCCCCTCCTGGCCAAGGCCCCCATAG
CCCACGCCGCGGCACCGTGGCTCTTCGGGGCGTTTCTCCTGGCCTTCGCCATCAAGTTCCCCCTCTTCCCGGTGCACACC
TGGCTCCCCGATGCCCACACCGACGCCCCCACGGCCGGGAGCGTGATCCTGGCGGCGCTCCTCCTGAAAACCGGGGCCTA
TGGGCTGGTTCGCTTCGGCTACCCCCTCTTTCCCGAGGCGGCCAAGGGGTTCACGCCCTTCATTTCGGCGCTGGCCGTCG
TCGGCATCCTCTACGCCTCGTGGGTCGCCTATGCCCAGGAGGACATGAAGCGGATGGTGGCCTACTCCAGCGTGGGGCAC
ATGGGGTTCGTGGCCCTGGGAATCGCCTCCTGGAGCCCGGTGGCCCTGTCCGGTTCCATCCTCCAGATGGTGAACCACGG
CGTCACCACCGGCGCCCTCTTCGCCCTGGTGGGGATGCTGGACGAACGGACCGATACCCGGGAGATCGCCGCCTACGGCG
GGGTGTGGGGGAAAGCGCCCAAGCTCGCCTTCTTCTTTCTCCTTTTCGCCATGACATCGGCGGGGTTGCCGGGGCTCAAC
AACTTCACGGGCGAATTCCTGATCCTTGCGGGGGTCTTCCGGACTACGCCGGCGGCAGCGGCCATCGCCTTTATCGGCAT
CGTGCTGCCGCTCATCTACACAGTGAGGCTCGTGCAGGAGGTGCTCTTCCAGACGGAGCGGCGGCCCCTGCGACTGCCGG
ACGTGACCCTGCGGGAGGAGGCGCTTCTGGCGGTGCTGGCGACGATCGACGTGTATATCGGTGTCCATCCGAAACCGCTC
CTGGATATCCTCAAGGTGCCGGTGGCGCTCCTGACGGGGGCGACGCCATGA

Upstream 100 bases:

>100_bases
GGGGGCTGCGTCTGCTTCTATCTCCTGATCCGCTTCCGCAATGAGCTGTGACGGTGGCGGGTTGCCACAAAACGTGATGT
CGTAAAACAGGTACTTGATC

Downstream 100 bases:

>100_bases
CCATAACCGAACTCTGGGCCCTCATCCCTCTTCTGATCCTCGCCTGCGGGAGCGTCTTCGTGCTCATGCTCGGGGCAATC
GTGCCGGGACGGTGCGGAAC

Product: proton-translocating NADH-quinone oxidoreductase, chain M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTDYPILTILIFLPLAGCLCLAPVWHCRTSARAVALGFAVAELALAAWLLVAATGMPAAPGAPTGYFLWEDAVWIERFGI
RYLLGMDGISLLMVALTAFITVVAMAVSWRAVTERTALHYFLILLMESGLMGVFLALDLVLFYLFWEVMLIPMFFLIGIW
GHGRRIYSAVKFFLYTLAGSLLMLLAIIGVYLIHGDATGIYTFTLPLLAKAPIAHAAAPWLFGAFLLAFAIKFPLFPVHT
WLPDAHTDAPTAGSVILAALLLKTGAYGLVRFGYPLFPEAAKGFTPFISALAVVGILYASWVAYAQEDMKRMVAYSSVGH
MGFVALGIASWSPVALSGSILQMVNHGVTTGALFALVGMLDERTDTREIAAYGGVWGKAPKLAFFFLLFAMTSAGLPGLN
NFTGEFLILAGVFRTTPAAAAIAFIGIVLPLIYTVRLVQEVLFQTERRPLRLPDVTLREEALLAVLATIDVYIGVHPKPL
LDILKVPVALLTGATP

Sequences:

>Translated_496_residues
MTDYPILTILIFLPLAGCLCLAPVWHCRTSARAVALGFAVAELALAAWLLVAATGMPAAPGAPTGYFLWEDAVWIERFGI
RYLLGMDGISLLMVALTAFITVVAMAVSWRAVTERTALHYFLILLMESGLMGVFLALDLVLFYLFWEVMLIPMFFLIGIW
GHGRRIYSAVKFFLYTLAGSLLMLLAIIGVYLIHGDATGIYTFTLPLLAKAPIAHAAAPWLFGAFLLAFAIKFPLFPVHT
WLPDAHTDAPTAGSVILAALLLKTGAYGLVRFGYPLFPEAAKGFTPFISALAVVGILYASWVAYAQEDMKRMVAYSSVGH
MGFVALGIASWSPVALSGSILQMVNHGVTTGALFALVGMLDERTDTREIAAYGGVWGKAPKLAFFFLLFAMTSAGLPGLN
NFTGEFLILAGVFRTTPAAAAIAFIGIVLPLIYTVRLVQEVLFQTERRPLRLPDVTLREEALLAVLATIDVYIGVHPKPL
LDILKVPVALLTGATP
>Mature_495_residues
TDYPILTILIFLPLAGCLCLAPVWHCRTSARAVALGFAVAELALAAWLLVAATGMPAAPGAPTGYFLWEDAVWIERFGIR
YLLGMDGISLLMVALTAFITVVAMAVSWRAVTERTALHYFLILLMESGLMGVFLALDLVLFYLFWEVMLIPMFFLIGIWG
HGRRIYSAVKFFLYTLAGSLLMLLAIIGVYLIHGDATGIYTFTLPLLAKAPIAHAAAPWLFGAFLLAFAIKFPLFPVHTW
LPDAHTDAPTAGSVILAALLLKTGAYGLVRFGYPLFPEAAKGFTPFISALAVVGILYASWVAYAQEDMKRMVAYSSVGHM
GFVALGIASWSPVALSGSILQMVNHGVTTGALFALVGMLDERTDTREIAAYGGVWGKAPKLAFFFLLFAMTSAGLPGLNN
FTGEFLILAGVFRTTPAAAAIAFIGIVLPLIYTVRLVQEVLFQTERRPLRLPDVTLREEALLAVLATIDVYIGVHPKPLL
DILKVPVALLTGATP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=301, Percent_Identity=34.21926910299, Blast_Score=165, Evalue=1e-40,
Organism=Escherichia coli, GI1788613, Length=488, Percent_Identity=41.1885245901639, Blast_Score=357, Evalue=1e-99,
Organism=Escherichia coli, GI1788831, Length=359, Percent_Identity=27.5766016713092, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1788827, Length=297, Percent_Identity=25.9259259259259, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788829, Length=343, Percent_Identity=29.4460641399417, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI145693160, Length=437, Percent_Identity=23.5697940503432, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788614, Length=461, Percent_Identity=26.6811279826464, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53636; Mature: 53505

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYPILTILIFLPLAGCLCLAPVWHCRTSARAVALGFAVAELALAAWLLVAATGMPAAP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GAPTGYFLWEDAVWIERFGIRYLLGMDGISLLMVALTAFITVVAMAVSWRAVTERTALHY
CCCCCCEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLILLMESGLMGVFLALDLVLFYLFWEVMLIPMFFLIGIWGHGRRIYSAVKFFLYTLAGS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LLMLLAIIGVYLIHGDATGIYTFTLPLLAKAPIAHAAAPWLFGAFLLAFAIKFPLFPVHT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
WLPDAHTDAPTAGSVILAALLLKTGAYGLVRFGYPLFPEAAKGFTPFISALAVVGILYAS
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHH
WVAYAQEDMKRMVAYSSVGHMGFVALGIASWSPVALSGSILQMVNHGVTTGALFALVGML
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
DERTDTREIAAYGGVWGKAPKLAFFFLLFAMTSAGLPGLNNFTGEFLILAGVFRTTPAAA
HCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHH
AIAFIGIVLPLIYTVRLVQEVLFQTERRPLRLPDVTLREEALLAVLATIDVYIGVHPKPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
LDILKVPVALLTGATP
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDYPILTILIFLPLAGCLCLAPVWHCRTSARAVALGFAVAELALAAWLLVAATGMPAAP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GAPTGYFLWEDAVWIERFGIRYLLGMDGISLLMVALTAFITVVAMAVSWRAVTERTALHY
CCCCCCEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLILLMESGLMGVFLALDLVLFYLFWEVMLIPMFFLIGIWGHGRRIYSAVKFFLYTLAGS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LLMLLAIIGVYLIHGDATGIYTFTLPLLAKAPIAHAAAPWLFGAFLLAFAIKFPLFPVHT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
WLPDAHTDAPTAGSVILAALLLKTGAYGLVRFGYPLFPEAAKGFTPFISALAVVGILYAS
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHH
WVAYAQEDMKRMVAYSSVGHMGFVALGIASWSPVALSGSILQMVNHGVTTGALFALVGML
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
DERTDTREIAAYGGVWGKAPKLAFFFLLFAMTSAGLPGLNNFTGEFLILAGVFRTTPAAA
HCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHH
AIAFIGIVLPLIYTVRLVQEVLFQTERRPLRLPDVTLREEALLAVLATIDVYIGVHPKPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
LDILKVPVALLTGATP
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]