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The map label for this gene is pyk [H]

Identifier: 78221349

GI number: 78221349

Start: 147911

End: 149353

Strand: Direct

Name: pyk [H]

Synonym: Gmet_0122

Alternate gene names: 78221349

Gene position: 147911-149353 (Clockwise)

Preceding gene: 78221348

Following gene: 78221350

Centisome position: 3.7

GC content: 65.42

Gene sequence:

>1443_bases
ATGGATAAATCGTCGCGCAAAACCAAGATCGTAGCCACCCTCGGACCGGCCAGCTCCTCGCCGGAGATGATCAGCAAGCT
CATGGAGGCAGGGGCAGACCTTTTCCGCCTCAACTTCTCCCACGGGGCCAACGACCAGCGCCGGGAGACCATTGCCACCA
TCCGGCGTCTTTCTGCCGCCCGGGGAAAGGAGATCGGCATCCTGGCCGACCTCCAGGGGCCAAAGATCAGGACCGGCCGC
ATGGAGAACGGCGCCATCTCCCTGCGCAAGGGAGACCCCCTCGACATCGTCACCGATGAGGTCCTCGGCACCCCGACCCT
CATCTCCACCATCTACAAGGCCCTCCCCCATGACGTGAAGCCCGGCTCCCGCATTCTCATGGACGACGGCCTCATCGAGC
TGAAGGTCCATTCGGTGGAGGGAAACCTGGTCCGCTGCACCGTGGTGGAGGGGGGAACCCTCAAGGACCTGAAGGGGATC
AACCTCCCCGGAGTCCATGTCTCGGCCCCCTCCCTCTCGGAGAAGGATCTGCGGGACCTGGAGTTCTGCCTGGCCGAGGG
GGTGGACTACATCGCCCTCTCCTTTGTCCGGAGCGCCGAGGACGTGGAGGGGCTCAAGCGGATCCTCTTCGAGCGCGGCA
TCCACATCCCCGTGGTGGCCAAGATCGAGAAGCCCGAGGCCCTGCGCAATTTCAAGAGCATCCTCAAAGCGGCCGACGGT
GTCATGGTGGCCCGGGGCGACCTGGGTGTTGAGATCAGCGCCGAGAAGGTCCCCCTCTTCCAGAAAAAAATCATCCGAGC
CTGCAACGAGGCGGGGAAGCCGGTTATCACCGCCACCCAGATGCTGGAATCGATGATCAGCCACCCGCGCCCCACCCGGG
CCGAGACCTCCGACGTGGCTAACGCCATCCTTGACGGGACCGACGCGGTCATGCTCTCGGGAGAGACCGCCTCGGGGCAG
TTCCCCCTGGAAGCGGTGCGGACCATGGCCAAGGTGGCCCTGGATGTGGAGCGCTACGCTCAGATGGAGGACACCCTCCC
CCAGCGGCGCCACAACCCGAGCATCGCCGAGGCGGTGGCCGAGGCCGCCTGCCGCGCCGCCGTCACCGTGGACGCCCGGG
CCATCTCGGTCATGACCCAGTCGGGGAGCACCGCCGCCCTCATCTCCAAGTTCCGGCCGCCGCTCCCCATCATCGCCTTT
ACCCAGTCCCTGGAGACCCGGCGGCGCCTCTCCCTCTACTGGGGGGTGAAGCCGCTCCCCATTGGCCCCATGACCGGCAC
CGACGAGCAGATCGCGGCCGTGGAATCGACGTTCCTCTCGGCGGGGTACCGCAAGGGAGACGTGATCGTCATAACCATGG
GGGTGCCGGTGGAGGCCCGAGGTTCCACGAACCTTATGAAGGTCCACAAGCTGGGGACGGGGAAGTTTTTCGAGATTTTC
TGA

Upstream 100 bases:

>100_bases
AAGGTGGACGAACTCCCCTGCTTCGAATGCCACAAATCCCCCACCCCGGCCCACAAGGGGCGGGTCGGGAAATTCCATCA
CTGAGGGAACCATCGTTTCC

Downstream 100 bases:

>100_bases
CGGAGGTTTGATTCAATGCGCAAGGTGTTCGACGAGACAAAGATCCACCACCTGACACTCCGCAACCGGCTGGTCCGCTC
CGCCACCTGGGAGGGGATGT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MDKSSRKTKIVATLGPASSSPEMISKLMEAGADLFRLNFSHGANDQRRETIATIRRLSAARGKEIGILADLQGPKIRTGR
MENGAISLRKGDPLDIVTDEVLGTPTLISTIYKALPHDVKPGSRILMDDGLIELKVHSVEGNLVRCTVVEGGTLKDLKGI
NLPGVHVSAPSLSEKDLRDLEFCLAEGVDYIALSFVRSAEDVEGLKRILFERGIHIPVVAKIEKPEALRNFKSILKAADG
VMVARGDLGVEISAEKVPLFQKKIIRACNEAGKPVITATQMLESMISHPRPTRAETSDVANAILDGTDAVMLSGETASGQ
FPLEAVRTMAKVALDVERYAQMEDTLPQRRHNPSIAEAVAEAACRAAVTVDARAISVMTQSGSTAALISKFRPPLPIIAF
TQSLETRRRLSLYWGVKPLPIGPMTGTDEQIAAVESTFLSAGYRKGDVIVITMGVPVEARGSTNLMKVHKLGTGKFFEIF

Sequences:

>Translated_480_residues
MDKSSRKTKIVATLGPASSSPEMISKLMEAGADLFRLNFSHGANDQRRETIATIRRLSAARGKEIGILADLQGPKIRTGR
MENGAISLRKGDPLDIVTDEVLGTPTLISTIYKALPHDVKPGSRILMDDGLIELKVHSVEGNLVRCTVVEGGTLKDLKGI
NLPGVHVSAPSLSEKDLRDLEFCLAEGVDYIALSFVRSAEDVEGLKRILFERGIHIPVVAKIEKPEALRNFKSILKAADG
VMVARGDLGVEISAEKVPLFQKKIIRACNEAGKPVITATQMLESMISHPRPTRAETSDVANAILDGTDAVMLSGETASGQ
FPLEAVRTMAKVALDVERYAQMEDTLPQRRHNPSIAEAVAEAACRAAVTVDARAISVMTQSGSTAALISKFRPPLPIIAF
TQSLETRRRLSLYWGVKPLPIGPMTGTDEQIAAVESTFLSAGYRKGDVIVITMGVPVEARGSTNLMKVHKLGTGKFFEIF
>Mature_480_residues
MDKSSRKTKIVATLGPASSSPEMISKLMEAGADLFRLNFSHGANDQRRETIATIRRLSAARGKEIGILADLQGPKIRTGR
MENGAISLRKGDPLDIVTDEVLGTPTLISTIYKALPHDVKPGSRILMDDGLIELKVHSVEGNLVRCTVVEGGTLKDLKGI
NLPGVHVSAPSLSEKDLRDLEFCLAEGVDYIALSFVRSAEDVEGLKRILFERGIHIPVVAKIEKPEALRNFKSILKAADG
VMVARGDLGVEISAEKVPLFQKKIIRACNEAGKPVITATQMLESMISHPRPTRAETSDVANAILDGTDAVMLSGETASGQ
FPLEAVRTMAKVALDVERYAQMEDTLPQRRHNPSIAEAVAEAACRAAVTVDARAISVMTQSGSTAALISKFRPPLPIIAF
TQSLETRRRLSLYWGVKPLPIGPMTGTDEQIAAVESTFLSAGYRKGDVIVITMGVPVEARGSTNLMKVHKLGTGKFFEIF

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=488, Percent_Identity=40.3688524590164, Blast_Score=345, Evalue=8e-95,
Organism=Homo sapiens, GI33286420, Length=488, Percent_Identity=40.3688524590164, Blast_Score=345, Evalue=8e-95,
Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=41.0204081632653, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=42.0408163265306, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI32967597, Length=488, Percent_Identity=41.3934426229508, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=40.8304498269896, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=40.8304498269896, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=35.2941176470588, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=35.2941176470588, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=34.4186046511628, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=44.0425531914894, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=41.25, Blast_Score=317, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17544584, Length=482, Percent_Identity=41.9087136929461, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=41.9087136929461, Blast_Score=321, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=41.9087136929461, Blast_Score=321, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=41.9087136929461, Blast_Score=320, Evalue=9e-88,
Organism=Caenorhabditis elegans, GI17506831, Length=482, Percent_Identity=41.9087136929461, Blast_Score=320, Evalue=1e-87,
Organism=Saccharomyces cerevisiae, GI6324923, Length=433, Percent_Identity=41.3394919168591, Blast_Score=299, Evalue=6e-82,
Organism=Saccharomyces cerevisiae, GI6319279, Length=483, Percent_Identity=38.7163561076605, Blast_Score=297, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24648964, Length=485, Percent_Identity=43.0927835051546, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI28571814, Length=485, Percent_Identity=43.0927835051546, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=42.6829268292683, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24581235, Length=427, Percent_Identity=37.9391100702576, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24646914, Length=282, Percent_Identity=40.4255319148936, Blast_Score=198, Evalue=6e-51,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51840; Mature: 51840

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSSRKTKIVATLGPASSSPEMISKLMEAGADLFRLNFSHGANDQRRETIATIRRLSAA
CCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
RGKEIGILADLQGPKIRTGRMENGAISLRKGDPLDIVTDEVLGTPTLISTIYKALPHDVK
CCCCEEEEEECCCCCEECCCCCCCEEEEECCCCCCEEHHHHCCCHHHHHHHHHHCCCCCC
PGSRILMDDGLIELKVHSVEGNLVRCTVVEGGTLKDLKGINLPGVHVSAPSLSEKDLRDL
CCCEEEEECCEEEEEEEEECCCEEEEEEECCCCHHHCCCCCCCCCEEECCCCCHHHHHHH
EFCLAEGVDYIALSFVRSAEDVEGLKRILFERGIHIPVVAKIEKPEALRNFKSILKAADG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCC
VMVARGDLGVEISAEKVPLFQKKIIRACNEAGKPVITATQMLESMISHPRPTRAETSDVA
EEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCHHHHH
NAILDGTDAVMLSGETASGQFPLEAVRTMAKVALDVERYAQMEDTLPQRRHNPSIAEAVA
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EAACRAAVTVDARAISVMTQSGSTAALISKFRPPLPIIAFTQSLETRRRLSLYWGVKPLP
HHHHHHHEEECCEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCCCC
IGPMTGTDEQIAAVESTFLSAGYRKGDVIVITMGVPVEARGSTNLMKVHKLGTGKFFEIF
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEECCCCCCEEEEEECCCCCEEEEC
>Mature Secondary Structure
MDKSSRKTKIVATLGPASSSPEMISKLMEAGADLFRLNFSHGANDQRRETIATIRRLSAA
CCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
RGKEIGILADLQGPKIRTGRMENGAISLRKGDPLDIVTDEVLGTPTLISTIYKALPHDVK
CCCCEEEEEECCCCCEECCCCCCCEEEEECCCCCCEEHHHHCCCHHHHHHHHHHCCCCCC
PGSRILMDDGLIELKVHSVEGNLVRCTVVEGGTLKDLKGINLPGVHVSAPSLSEKDLRDL
CCCEEEEECCEEEEEEEEECCCEEEEEEECCCCHHHCCCCCCCCCEEECCCCCHHHHHHH
EFCLAEGVDYIALSFVRSAEDVEGLKRILFERGIHIPVVAKIEKPEALRNFKSILKAADG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCC
VMVARGDLGVEISAEKVPLFQKKIIRACNEAGKPVITATQMLESMISHPRPTRAETSDVA
EEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCHHHHH
NAILDGTDAVMLSGETASGQFPLEAVRTMAKVALDVERYAQMEDTLPQRRHNPSIAEAVA
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EAACRAAVTVDARAISVMTQSGSTAALISKFRPPLPIIAFTQSLETRRRLSLYWGVKPLP
HHHHHHHEEECCEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCCCC
IGPMTGTDEQIAAVESTFLSAGYRKGDVIVITMGVPVEARGSTNLMKVHKLGTGKFFEIF
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEECCCCCCEEEEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]