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The map label for this gene is fadH [C]

Identifier: 78221350

GI number: 78221350

Start: 149369

End: 150493

Strand: Direct

Name: fadH [C]

Synonym: Gmet_0123

Alternate gene names: 78221350

Gene position: 149369-150493 (Clockwise)

Preceding gene: 78221349

Following gene: 78221351

Centisome position: 3.74

GC content: 67.82

Gene sequence:

>1125_bases
ATGCGCAAGGTGTTCGACGAGACAAAGATCCACCACCTGACACTCCGCAACCGGCTGGTCCGCTCCGCCACCTGGGAGGG
GATGTGCCGGGCCGGAGGGCATCCCACGGAGAAGCTGGCCGCCTACTACGGGACCCTGGCCCGGGGCGGGGTGGGGCTCA
TCATCTCCGGCTACGCCTTCGTCCGTCCCGACGGAAAGCAGCTTCCCGGACAGCTGGGAATCCACGAGGACTCCTTTGCC
CCCGAGGCACGGCTCCTGACCGAGGCGGTCCACCGGGAGGGGGGTAAAATCTGTCTCCAGCTGGTCCACTGCGGCGGCCA
GACCACGGCCAGGGCGGCCGGCGCTCAGCCAGTGGCCCCTACCGCCGTGCAAATCGATCAATACCCGGAACTTCCCCGTG
AACTCCCCGAGGCCGAGATCGCGGATCTGGTGGCAGCCTTCGCCGCCGGCGCCGTCCGGGCCCGGGAGTGGGGCTTCGAC
GCGGTCCAGCTCCATGCCGCCCACGGCTACCTCATCAACCAGTTCCTCTCCCCCCTCACCAACCGCCGCACCGACCGCTA
CGGCGGCTCCCTGGAGAACCGGTGCCGCTTTCTCCTGGAGATTTCCCATGCTGTACGTGCCGCCGTGGGGAAGGATTTCC
CGGTCATGGTGAAACTCAACGGCTCCGACAACCTGAACGGGGGACTCGACCTGGAGGAGGCGGTTCTGGTGGCCCGGATG
CTGGATGAGGAGGGGATCGACGCCATCGAGGTCTCCGGCGGCACACCGGCCTCGGGCGGACAGACCCCGGTCCGCCAGGG
GATCGAGACCCGGGAGCAGGAGGCCTACAACCTCCCCCTCGCCTACCGGATCAAGCAGGTGGTCTCCTGCCCCGTCATGG
TGGTGGGGGGGTTCCGCACCTTCGAGCTGGTGGAAGGGGTCATCCGACGGGAAGAGGCCGACTTTGTCTCCCTTTCCCGC
CCCCTCATCCGGGAGCCCAACCTCCCTCGCCGCTGGGAGGAGGGGGACGAGAGCCGGGCCCGCTGCATCTCCTGCAACGG
CTGCTTCAAGCCGGGGATGAAGGAAGGGGGAATCTACTGCGTGGTGGACAAGGTGGAGCGGGAGAGCCGGGCGTTTTCCC
TCTGA

Upstream 100 bases:

>100_bases
GGGGGTGCCGGTGGAGGCCCGAGGTTCCACGAACCTTATGAAGGTCCACAAGCTGGGGACGGGGAAGTTTTTCGAGATTT
TCTGACGGAGGTTTGATTCA

Downstream 100 bases:

>100_bases
GGCGATACATTAGCGACACATTCCGTCACAGGCCCGGGGTAGGATTGCCCGGGCCTTGATTATTCTGACGGGAAACAGTG
AGCACCATGCCCGAACTCAG

Product: flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MRKVFDETKIHHLTLRNRLVRSATWEGMCRAGGHPTEKLAAYYGTLARGGVGLIISGYAFVRPDGKQLPGQLGIHEDSFA
PEARLLTEAVHREGGKICLQLVHCGGQTTARAAGAQPVAPTAVQIDQYPELPRELPEAEIADLVAAFAAGAVRAREWGFD
AVQLHAAHGYLINQFLSPLTNRRTDRYGGSLENRCRFLLEISHAVRAAVGKDFPVMVKLNGSDNLNGGLDLEEAVLVARM
LDEEGIDAIEVSGGTPASGGQTPVRQGIETREQEAYNLPLAYRIKQVVSCPVMVVGGFRTFELVEGVIRREEADFVSLSR
PLIREPNLPRRWEEGDESRARCISCNGCFKPGMKEGGIYCVVDKVERESRAFSL

Sequences:

>Translated_374_residues
MRKVFDETKIHHLTLRNRLVRSATWEGMCRAGGHPTEKLAAYYGTLARGGVGLIISGYAFVRPDGKQLPGQLGIHEDSFA
PEARLLTEAVHREGGKICLQLVHCGGQTTARAAGAQPVAPTAVQIDQYPELPRELPEAEIADLVAAFAAGAVRAREWGFD
AVQLHAAHGYLINQFLSPLTNRRTDRYGGSLENRCRFLLEISHAVRAAVGKDFPVMVKLNGSDNLNGGLDLEEAVLVARM
LDEEGIDAIEVSGGTPASGGQTPVRQGIETREQEAYNLPLAYRIKQVVSCPVMVVGGFRTFELVEGVIRREEADFVSLSR
PLIREPNLPRRWEEGDESRARCISCNGCFKPGMKEGGIYCVVDKVERESRAFSL
>Mature_374_residues
MRKVFDETKIHHLTLRNRLVRSATWEGMCRAGGHPTEKLAAYYGTLARGGVGLIISGYAFVRPDGKQLPGQLGIHEDSFA
PEARLLTEAVHREGGKICLQLVHCGGQTTARAAGAQPVAPTAVQIDQYPELPRELPEAEIADLVAAFAAGAVRAREWGFD
AVQLHAAHGYLINQFLSPLTNRRTDRYGGSLENRCRFLLEISHAVRAAVGKDFPVMVKLNGSDNLNGGLDLEEAVLVARM
LDEEGIDAIEVSGGTPASGGQTPVRQGIETREQEAYNLPLAYRIKQVVSCPVMVVGGFRTFELVEGVIRREEADFVSLSR
PLIREPNLPRRWEEGDESRARCISCNGCFKPGMKEGGIYCVVDKVERESRAFSL

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=357, Percent_Identity=34.1736694677871, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787939, Length=349, Percent_Identity=30.945558739255, Blast_Score=128, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI72001454, Length=348, Percent_Identity=33.9080459770115, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17559804, Length=361, Percent_Identity=31.0249307479224, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17564188, Length=347, Percent_Identity=30.835734870317, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17565138, Length=336, Percent_Identity=31.547619047619, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17559802, Length=342, Percent_Identity=31.2865497076023, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17540738, Length=344, Percent_Identity=30.2325581395349, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17566914, Length=337, Percent_Identity=29.9703264094956, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17566916, Length=345, Percent_Identity=24.6376811594203, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=222, Percent_Identity=29.2792792792793, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321973, Length=181, Percent_Identity=32.0441988950276, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 40962; Mature: 40962

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVFDETKIHHLTLRNRLVRSATWEGMCRAGGHPTEKLAAYYGTLARGGVGLIISGYAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECEEE
VRPDGKQLPGQLGIHEDSFAPEARLLTEAVHREGGKICLQLVHCGGQTTARAAGAQPVAP
ECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHCCCCCCCC
TAVQIDQYPELPRELPEAEIADLVAAFAAGAVRAREWGFDAVQLHAAHGYLINQFLSPLT
CEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHH
NRRTDRYGGSLENRCRFLLEISHAVRAAVGKDFPVMVKLNGSDNLNGGLDLEEAVLVARM
HCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
LDEEGIDAIEVSGGTPASGGQTPVRQGIETREQEAYNLPLAYRIKQVVSCPVMVVGGFRT
HHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCHH
FELVEGVIRREEADFVSLSRPLIREPNLPRRWEEGDESRARCISCNGCFKPGMKEGGIYC
HHHHHHHHHHCCCCHHHHCCCHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEE
VVDKVERESRAFSL
EEECHHHHHHCCCC
>Mature Secondary Structure
MRKVFDETKIHHLTLRNRLVRSATWEGMCRAGGHPTEKLAAYYGTLARGGVGLIISGYAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECEEE
VRPDGKQLPGQLGIHEDSFAPEARLLTEAVHREGGKICLQLVHCGGQTTARAAGAQPVAP
ECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHCCCCCCCC
TAVQIDQYPELPRELPEAEIADLVAAFAAGAVRAREWGFDAVQLHAAHGYLINQFLSPLT
CEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHH
NRRTDRYGGSLENRCRFLLEISHAVRAAVGKDFPVMVKLNGSDNLNGGLDLEEAVLVARM
HCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
LDEEGIDAIEVSGGTPASGGQTPVRQGIETREQEAYNLPLAYRIKQVVSCPVMVVGGFRT
HHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCHH
FELVEGVIRREEADFVSLSRPLIREPNLPRRWEEGDESRARCISCNGCFKPGMKEGGIYC
HHHHHHHHHHCCCCHHHHCCCHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEE
VVDKVERESRAFSL
EEECHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]