Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 78221296

GI number: 78221296

Start: 83666

End: 84610

Strand: Direct

Name: pleD [H]

Synonym: Gmet_0069

Alternate gene names: 78221296

Gene position: 83666-84610 (Clockwise)

Preceding gene: 78221295

Following gene: 78221308

Centisome position: 2.09

GC content: 61.69

Gene sequence:

>945_bases
ATGAAGAAGACAGTCTTGGTTATCGACGACTCGGACACCCTGCGGGAGGAGATCCTCCGGTCACTCCGGGGAGTATCCCT
TTTCGACACCTACCTGGAGGCCGGTGACGGGCTTGACGGGTTCAAGGTTCTCCTCAACAACCGGGTGGACCTGGTCCTCT
GCGACCTGGAAATGCCGCGGATGGACGGCTTCCGTTTTCTTACCATGATGCGGGCCCGGGAGGAGTTTCAGGACCTGCCG
GTCATCATCCTGACGGGGCGTGAGGACCGGGACACGAAGATTCGTGGCCTTGAGCAGGGGGCTTCCGACTTCGTGACCAA
ACCGTTCGACACCGGGGAACTGGTGGCCCGGGTGCGGGTCCAACTCAAGATGAAGGGACTTCAGGACGAGTTGAAGCGCT
CCAACGAGATGCTGCGTACCCTCTCCATCACCGATCCCCTGACCCACCTCCACAACCGCCGCCACCTCATGGAGATGGTG
GATAAGGAGTTCCAGCGCTCCTCCCGCAAGGGGGCGCCCCTCTCCCTCGTCATCCTCGACATCGACTACTTCAAGAAAAT
CAACGATACCTACGGGCACCAGGAGGGGGACCGGGTCCTCACCATCCTGGCCGACATCGTCCGAAGGCGGCTTCGGAGCT
ACGACCTCGCGGCCCGCTACGGCGGGGAAGAGTTCGTGCTGCTGCTGCCGGAAACTCCCGTCCAAGAGGCCCTCTCCATA
GCTGAGCGGTTGCGGCTGGAGGTTCAGGAACATGTCTTCGAGGGAAGCCTCGGGGGGCTGGTGATAACCATCAGTCTTGG
CGTGGCCACGTACCCGTCGTCCCGGGTGGAGAGCATCGACTCGCTCTTCAGGCAGGCCGACGAGGCACTCTACCGTGCCA
AACAGGGGGGACGCAACCGCGTTGAGCTCATGGCAGGGGCAAGCATTGAAAAAGGGGTGCCTTAA

Upstream 100 bases:

>100_bases
CGCTGCCATCCTCAAGCAGTTCCTGGATCTGACGTTCCAGCGGTAACGTGTGGAGCAGGAGAAATCGCGTAGCCAGGTGC
TACGGGAGCATAGGGCTGAA

Downstream 100 bases:

>100_bases
GGCGCTCAGGTGCTCGTTTCTGACTGACGGAATTATGATGCCGGCCGTTCCGTAACGTGGCTTCGACAGAAGAGGTGGAG
GCCACAGCCGGGGCAGAGGG

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKKTVLVIDDSDTLREEILRSLRGVSLFDTYLEAGDGLDGFKVLLNNRVDLVLCDLEMPRMDGFRFLTMMRAREEFQDLP
VIILTGREDRDTKIRGLEQGASDFVTKPFDTGELVARVRVQLKMKGLQDELKRSNEMLRTLSITDPLTHLHNRRHLMEMV
DKEFQRSSRKGAPLSLVILDIDYFKKINDTYGHQEGDRVLTILADIVRRRLRSYDLAARYGGEEFVLLLPETPVQEALSI
AERLRLEVQEHVFEGSLGGLVITISLGVATYPSSRVESIDSLFRQADEALYRAKQGGRNRVELMAGASIEKGVP

Sequences:

>Translated_314_residues
MKKTVLVIDDSDTLREEILRSLRGVSLFDTYLEAGDGLDGFKVLLNNRVDLVLCDLEMPRMDGFRFLTMMRAREEFQDLP
VIILTGREDRDTKIRGLEQGASDFVTKPFDTGELVARVRVQLKMKGLQDELKRSNEMLRTLSITDPLTHLHNRRHLMEMV
DKEFQRSSRKGAPLSLVILDIDYFKKINDTYGHQEGDRVLTILADIVRRRLRSYDLAARYGGEEFVLLLPETPVQEALSI
AERLRLEVQEHVFEGSLGGLVITISLGVATYPSSRVESIDSLFRQADEALYRAKQGGRNRVELMAGASIEKGVP
>Mature_314_residues
MKKTVLVIDDSDTLREEILRSLRGVSLFDTYLEAGDGLDGFKVLLNNRVDLVLCDLEMPRMDGFRFLTMMRAREEFQDLP
VIILTGREDRDTKIRGLEQGASDFVTKPFDTGELVARVRVQLKMKGLQDELKRSNEMLRTLSITDPLTHLHNRRHLMEMV
DKEFQRSSRKGAPLSLVILDIDYFKKINDTYGHQEGDRVLTILADIVRRRLRSYDLAARYGGEEFVLLLPETPVQEALSI
AERLRLEVQEHVFEGSLGGLVITISLGVATYPSSRVESIDSLFRQADEALYRAKQGGRNRVELMAGASIEKGVP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=45.0867052023121, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=40.2439024390244, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=41.4201183431953, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787262, Length=159, Percent_Identity=37.7358490566038, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786584, Length=175, Percent_Identity=35.4285714285714, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787802, Length=163, Percent_Identity=35.5828220858896, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787816, Length=168, Percent_Identity=38.6904761904762, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1788381, Length=187, Percent_Identity=37.4331550802139, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=31.4465408805031, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=35.2272727272727, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788085, Length=215, Percent_Identity=31.6279069767442, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI87082012, Length=167, Percent_Identity=32.3353293413174, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790299, Length=114, Percent_Identity=37.719298245614, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788550, Length=136, Percent_Identity=32.3529411764706, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786599, Length=123, Percent_Identity=32.520325203252, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788394, Length=131, Percent_Identity=31.2977099236641, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788191, Length=109, Percent_Identity=35.7798165137615, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87081977, Length=174, Percent_Identity=26.4367816091954, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786911, Length=120, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=164, Percent_Identity=29.8780487804878, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786784, Length=90, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788956, Length=233, Percent_Identity=29.1845493562232, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787229, Length=86, Percent_Identity=43.0232558139535, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790552, Length=80, Percent_Identity=45, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790863, Length=115, Percent_Identity=33.9130434782609, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35698; Mature: 35698

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTVLVIDDSDTLREEILRSLRGVSLFDTYLEAGDGLDGFKVLLNNRVDLVLCDLEMPR
CCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCCC
MDGFRFLTMMRAREEFQDLPVIILTGREDRDTKIRGLEQGASDFVTKPFDTGELVARVRV
CCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCHHHHCCCCCCHHHHHHHHHH
QLKMKGLQDELKRSNEMLRTLSITDPLTHLHNRRHLMEMVDKEFQRSSRKGAPLSLVILD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
IDYFKKINDTYGHQEGDRVLTILADIVRRRLRSYDLAARYGGEEFVLLLPETPVQEALSI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
AERLRLEVQEHVFEGSLGGLVITISLGVATYPSSRVESIDSLFRQADEALYRAKQGGRNR
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
VELMAGASIEKGVP
EEEEECCCCCCCCC
>Mature Secondary Structure
MKKTVLVIDDSDTLREEILRSLRGVSLFDTYLEAGDGLDGFKVLLNNRVDLVLCDLEMPR
CCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCCC
MDGFRFLTMMRAREEFQDLPVIILTGREDRDTKIRGLEQGASDFVTKPFDTGELVARVRV
CCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCHHHHCCCCCCHHHHHHHHHH
QLKMKGLQDELKRSNEMLRTLSITDPLTHLHNRRHLMEMVDKEFQRSSRKGAPLSLVILD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
IDYFKKINDTYGHQEGDRVLTILADIVRRRLRSYDLAARYGGEEFVLLLPETPVQEALSI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
AERLRLEVQEHVFEGSLGGLVITISLGVATYPSSRVESIDSLFRQADEALYRAKQGGRNR
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
VELMAGASIEKGVP
EEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA