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The map label for this gene is rsmB [H]

Identifier: 78221293

GI number: 78221293

Start: 80968

End: 82308

Strand: Direct

Name: rsmB [H]

Synonym: Gmet_0066

Alternate gene names: 78221293

Gene position: 80968-82308 (Clockwise)

Preceding gene: 78221291

Following gene: 78221294

Centisome position: 2.03

GC content: 67.49

Gene sequence:

>1341_bases
GTGACGAGAAGCAATCCGCGCCGAACGGCGCTCGACATCCTTCTGCGGGTCGAACGGGACCGCGCCTTTGCCGAGCAGCT
CCTGGACCGGGAGCTTTCCCTGGGGACCCTTACGGGGCCCGACCGGGGGCTTCTGACTGAGCTGGTCTACGGGGTGCTCC
GCCGCCAGGGAACCCTCGACTTTCTGGTGGACGCCTTTGCCGCCAGCCGCAGCGGCAAGCTGGAGCGGGCGATCCTGGTC
ATCCTTCGGCTCGGGCTCTATCAGCTCTTCTTCCTGGACCGGATCCCCACTTCGGCCGCCGTGAACGAATCGGTGAACCT
GGCCAAGGCCGTGGCGCCCCGGGCCGCGGGGTTCGTGAACGCGGTGCTGCGCCGGGCCGACCGGGAGCGGGACTCCCTCC
CCTGGCCCGATCGCGGGGCTGACCCCGCTGCCTACATCGCGGTGCGTCACTCCCATCCCCGCTGGCTCGTGGAGCAGTGG
ATCGAGCAGCTCGGCATCCCGGAAGCGGAGGAGCTTGCGGTCGCCATGGCCGAGCAGGCCCCCCTTACCATCCGGGTCAA
TACGCTGAAGACCACCCGAGAGGCCTTCATGGAGGAGCTTGCCGCCGCCGGGATCACGGCTGAGCCCACCGTCTGGGCGC
CCCACGGGGTTCGCATCCTCTCCCGTACGGCAGTCACCACCCTTCCCGGCTTTGCCGAGGGGCTCTTTACGGTGCAGGAC
GAGTCGTCGCAGCTGGCGGCGTTTTTTCTCGATCCCCAACCCGGCGAGCGGATTCTCGACGTCTGTGCCGCCCCCGGGGG
CAAGGCCACGTACCTGGCCCAGCTCATGGAAAACCGGGGAGAGGTCCTGGCCTGCGACATCTCGGCCAAGAAGCTCCGGC
TCGTGGCCGAGGCTGCGGCCCGGCTCGGCATCGGCATCATCCGCACCATGGCCGGCGACGCAACGAAGCCCGGTGCCGGT
GAGCCCCCCTTCGACCGGATCCTCCTCGATGCCCCCTGTTCGGGATTCGGCGTCATCCGCCGCAATCCCGAGGGGAAGTG
GTGGAAGACCCCCGACGATCTGGCCCGTCTCGCCGCCACCCAGCGGAACATCCTCGCTGCCGTCGCCCCGAGGCTCCGGC
CCGGCGGGGTCCTCCTCTACTCCACCTGCTCGACCATGGTGGCCGAGGACGAAGACGTCGTGGATGATTTCCTTTCCCGG
CACGAAGATTTTGTGTTAGAAGATTTGAATGCCATTTCCCCCGATTTTCCCGGACAATTTACCGGTCGGGGGCATTTCCG
GAGCTGGCCCCACCGGCACGGCATGGACGGTTTTTTCGCCGCCCGGATCAGAAAGCTCTAG

Upstream 100 bases:

>100_bases
CACCGTCCGGTCAAGTCAAGCAGCAGGCCGAAGCCGTTTCTTGAGGTTTCGGGCGGGAATTACTATACTCAGAACCAATA
CAACGATAAAGGAATTATCC

Downstream 100 bases:

>100_bases
CACGTGGAGGATGCAATGAAGAAGATAGCGCCATCAATTCTGTCCGCCGATTTTTCCCGGCTCGGCGACGAGGTCAGGGC
CGTGGCAGCCGCCGGGGCCG

Product: Fmu, rRNA SAM-dependent methyltransferase:Nop2p

Products: NA

Alternate protein names: rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTRSNPRRTALDILLRVERDRAFAEQLLDRELSLGTLTGPDRGLLTELVYGVLRRQGTLDFLVDAFAASRSGKLERAILV
ILRLGLYQLFFLDRIPTSAAVNESVNLAKAVAPRAAGFVNAVLRRADRERDSLPWPDRGADPAAYIAVRHSHPRWLVEQW
IEQLGIPEAEELAVAMAEQAPLTIRVNTLKTTREAFMEELAAAGITAEPTVWAPHGVRILSRTAVTTLPGFAEGLFTVQD
ESSQLAAFFLDPQPGERILDVCAAPGGKATYLAQLMENRGEVLACDISAKKLRLVAEAAARLGIGIIRTMAGDATKPGAG
EPPFDRILLDAPCSGFGVIRRNPEGKWWKTPDDLARLAATQRNILAAVAPRLRPGGVLLYSTCSTMVAEDEDVVDDFLSR
HEDFVLEDLNAISPDFPGQFTGRGHFRSWPHRHGMDGFFAARIRKL

Sequences:

>Translated_446_residues
MTRSNPRRTALDILLRVERDRAFAEQLLDRELSLGTLTGPDRGLLTELVYGVLRRQGTLDFLVDAFAASRSGKLERAILV
ILRLGLYQLFFLDRIPTSAAVNESVNLAKAVAPRAAGFVNAVLRRADRERDSLPWPDRGADPAAYIAVRHSHPRWLVEQW
IEQLGIPEAEELAVAMAEQAPLTIRVNTLKTTREAFMEELAAAGITAEPTVWAPHGVRILSRTAVTTLPGFAEGLFTVQD
ESSQLAAFFLDPQPGERILDVCAAPGGKATYLAQLMENRGEVLACDISAKKLRLVAEAAARLGIGIIRTMAGDATKPGAG
EPPFDRILLDAPCSGFGVIRRNPEGKWWKTPDDLARLAATQRNILAAVAPRLRPGGVLLYSTCSTMVAEDEDVVDDFLSR
HEDFVLEDLNAISPDFPGQFTGRGHFRSWPHRHGMDGFFAARIRKL
>Mature_445_residues
TRSNPRRTALDILLRVERDRAFAEQLLDRELSLGTLTGPDRGLLTELVYGVLRRQGTLDFLVDAFAASRSGKLERAILVI
LRLGLYQLFFLDRIPTSAAVNESVNLAKAVAPRAAGFVNAVLRRADRERDSLPWPDRGADPAAYIAVRHSHPRWLVEQWI
EQLGIPEAEELAVAMAEQAPLTIRVNTLKTTREAFMEELAAAGITAEPTVWAPHGVRILSRTAVTTLPGFAEGLFTVQDE
SSQLAAFFLDPQPGERILDVCAAPGGKATYLAQLMENRGEVLACDISAKKLRLVAEAAARLGIGIIRTMAGDATKPGAGE
PPFDRILLDAPCSGFGVIRRNPEGKWWKTPDDLARLAATQRNILAAVAPRLRPGGVLLYSTCSTMVAEDEDVVDDFLSRH
EDFVLEDLNAISPDFPGQFTGRGHFRSWPHRHGMDGFFAARIRKL

Specific function: Unknown

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=322, Percent_Identity=35.4037267080745, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI76150623, Length=322, Percent_Identity=35.4037267080745, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI32698918, Length=218, Percent_Identity=39.9082568807339, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI8922322, Length=287, Percent_Identity=31.7073170731707, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI270288818, Length=287, Percent_Identity=31.7073170731707, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI270288816, Length=287, Percent_Identity=31.7073170731707, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI23199998, Length=287, Percent_Identity=31.7073170731707, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI39995082, Length=311, Percent_Identity=30.2250803858521, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI301336155, Length=284, Percent_Identity=31.3380281690141, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI11545785, Length=200, Percent_Identity=29, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI2367212, Length=433, Percent_Identity=35.796766743649, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87081985, Length=283, Percent_Identity=38.5159010600707, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17536757, Length=285, Percent_Identity=36.140350877193, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI71998419, Length=299, Percent_Identity=28.4280936454849, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI115534021, Length=218, Percent_Identity=32.5688073394495, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25143471, Length=271, Percent_Identity=30.2583025830258, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324268, Length=306, Percent_Identity=34.640522875817, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319447, Length=216, Percent_Identity=31.4814814814815, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI22024126, Length=314, Percent_Identity=33.4394904458599, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24668781, Length=198, Percent_Identity=38.3838383838384, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356513, Length=198, Percent_Identity=38.3838383838384, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356579, Length=260, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21355201, Length=223, Percent_Identity=30.4932735426009, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: 2.1.1.-

Molecular weight: Translated: 49001; Mature: 48870

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSNPRRTALDILLRVERDRAFAEQLLDRELSLGTLTGPDRGLLTELVYGVLRRQGTLD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHH
FLVDAFAASRSGKLERAILVILRLGLYQLFFLDRIPTSAAVNESVNLAKAVAPRAAGFVN
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHH
AVLRRADRERDSLPWPDRGADPAAYIAVRHSHPRWLVEQWIEQLGIPEAEELAVAMAEQA
HHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCC
PLTIRVNTLKTTREAFMEELAAAGITAEPTVWAPHGVRILSRTAVTTLPGFAEGLFTVQD
CEEEEEECHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHCCCHHHCEEEEEC
ESSQLAAFFLDPQPGERILDVCAAPGGKATYLAQLMENRGEVLACDISAKKLRLVAEAAA
CCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
RLGIGIIRTMAGDATKPGAGEPPFDRILLDAPCSGFGVIRRNPEGKWWKTPDDLARLAAT
HHHHHHHHHHCCCCCCCCCCCCCHHHEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHH
QRNILAAVAPRLRPGGVLLYSTCSTMVAEDEDVVDDFLSRHEDFVLEDLNAISPDFPGQF
HHHHHHHHCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TGRGHFRSWPHRHGMDGFFAARIRKL
CCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TRSNPRRTALDILLRVERDRAFAEQLLDRELSLGTLTGPDRGLLTELVYGVLRRQGTLD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHH
FLVDAFAASRSGKLERAILVILRLGLYQLFFLDRIPTSAAVNESVNLAKAVAPRAAGFVN
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHH
AVLRRADRERDSLPWPDRGADPAAYIAVRHSHPRWLVEQWIEQLGIPEAEELAVAMAEQA
HHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCC
PLTIRVNTLKTTREAFMEELAAAGITAEPTVWAPHGVRILSRTAVTTLPGFAEGLFTVQD
CEEEEEECHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHCCCHHHCEEEEEC
ESSQLAAFFLDPQPGERILDVCAAPGGKATYLAQLMENRGEVLACDISAKKLRLVAEAAA
CCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
RLGIGIIRTMAGDATKPGAGEPPFDRILLDAPCSGFGVIRRNPEGKWWKTPDDLARLAAT
HHHHHHHHHHCCCCCCCCCCCCCHHHEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHH
QRNILAAVAPRLRPGGVLLYSTCSTMVAEDEDVVDDFLSRHEDFVLEDLNAISPDFPGQF
HHHHHHHHCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TGRGHFRSWPHRHGMDGFFAARIRKL
CCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]