Click here to switch to the map view.

The map label for this gene is ispD

Identifier: 78221287

GI number: 78221287

Start: 74326

End: 75024

Strand: Reverse

Name: ispD

Synonym: Gmet_0060

Alternate gene names: 78221287

Gene position: 75024-74326 (Counterclockwise)

Preceding gene: 78221288

Following gene: 78221286

Centisome position: 1.88

GC content: 64.66

Gene sequence:

>699_bases
ATGGCAGTTTTCGCCCTGATTCCAGCGGCAGGCATGGGCAAGCGGATGGGTGCCTCCATCAACAAGCAGTATCTGCTCCT
GGGCGGAATGCCGATCCTTGCCCGCACCCTGGAAGTCTTCGAGCGCTCCCCCCTGGTGGACGGCATCTTCGTCGTGATTC
CAGCGGACGAGATCCCTTTCTGCCGGGAGCAGGTGGTGGAGCGGCACGGCTTTTCGAAGGTGCGGAGCATCGTTGCCGGC
GGAGCCGAGCGTCAGCAGTCGGTACTGAACGGCCTGCGGGCCATGGAGGGGACGGCGGGGGAGTACGACGTGATTCTCAT
CCACGACGGGGTCCGCCCCTTTGTCACGGAGGATATCCTCGGCCGGGCAACCGCAACGGCCCGGGAAAACGACGGAGCCC
TTGTGGCGGTACCGGCCAAGGACACGGTGAAGGTGGTGGAGGACGGCATCATCACCGCAACGCCGTCACGGGAGACCCTC
TGGCTCGCCCAGACTCCCCAGGCCTTCCGCTACGGCGTCATCCGCGCAGCCCACGAGGTGGCCGACGCCGAGCGGTTCCT
CGGCACCGACGACGCCATGCTCGTGGAACGCCTGGGGCGACAGGTACGGATCGTTCTGGGAGACTACCGCAACGTTAAGA
TCACGACGCCGGAGGATATGGTTCTGGCAGAAGCGTTCATCAAGGAGAAGCTGCCATGA

Upstream 100 bases:

>100_bases
TGGCGCGACTTTTGTCTGGTCTTTTGCCTATCCTTTTGTATAATCGGATAATTCCGGCCGGCTGCTCCCGCAGCGCACCG
GCCCGTAATGAGGTCGACAC

Downstream 100 bases:

>100_bases
GAATCGGGCACGGCTACGACGTCCATTGCCTCGTGGAGGGGCGGAAACTGATTCTCGGAGGAGTTGATGTTCCCTACGAA
CGGGGGCTTTTGGGACACTC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MAVFALIPAAGMGKRMGASINKQYLLLGGMPILARTLEVFERSPLVDGIFVVIPADEIPFCREQVVERHGFSKVRSIVAG
GAERQQSVLNGLRAMEGTAGEYDVILIHDGVRPFVTEDILGRATATARENDGALVAVPAKDTVKVVEDGIITATPSRETL
WLAQTPQAFRYGVIRAAHEVADAERFLGTDDAMLVERLGRQVRIVLGDYRNVKITTPEDMVLAEAFIKEKLP

Sequences:

>Translated_232_residues
MAVFALIPAAGMGKRMGASINKQYLLLGGMPILARTLEVFERSPLVDGIFVVIPADEIPFCREQVVERHGFSKVRSIVAG
GAERQQSVLNGLRAMEGTAGEYDVILIHDGVRPFVTEDILGRATATARENDGALVAVPAKDTVKVVEDGIITATPSRETL
WLAQTPQAFRYGVIRAAHEVADAERFLGTDDAMLVERLGRQVRIVLGDYRNVKITTPEDMVLAEAFIKEKLP
>Mature_231_residues
AVFALIPAAGMGKRMGASINKQYLLLGGMPILARTLEVFERSPLVDGIFVVIPADEIPFCREQVVERHGFSKVRSIVAGG
AERQQSVLNGLRAMEGTAGEYDVILIHDGVRPFVTEDILGRATATARENDGALVAVPAKDTVKVVEDGIITATPSRETLW
LAQTPQAFRYGVIRAAHEVADAERFLGTDDAMLVERLGRQVRIVLGDYRNVKITTPEDMVLAEAFIKEKLP

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=238, Percent_Identity=28.9915966386555, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI157671913, Length=134, Percent_Identity=30.5970149253731, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1789104, Length=235, Percent_Identity=34.0425531914894, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_GEOMG (Q39ZL5)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383034.1
- ProteinModelPortal:   Q39ZL5
- STRING:   Q39ZL5
- GeneID:   3738548
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0060
- NMPDR:   fig|269799.3.peg.123
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- PhylomeDB:   Q39ZL5
- ProtClustDB:   PRK00155
- BioCyc:   GMET269799:GMET_0060-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25299; Mature: 25167

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFALIPAAGMGKRMGASINKQYLLLGGMPILARTLEVFERSPLVDGIFVVIPADEIPF
CEEEEEECCCCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCH
CREQVVERHGFSKVRSIVAGGAERQQSVLNGLRAMEGTAGEYDVILIHDGVRPFVTEDIL
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHH
GRATATARENDGALVAVPAKDTVKVVEDGIITATPSRETLWLAQTPQAFRYGVIRAAHEV
HHHHCCCCCCCCCEEEECCCCHHHHHHCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHH
ADAERFLGTDDAMLVERLGRQVRIVLGDYRNVKITTPEDMVLAEAFIKEKLP
HHHHHHCCCCHHHHHHHCCCEEEEEEECCCEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVFALIPAAGMGKRMGASINKQYLLLGGMPILARTLEVFERSPLVDGIFVVIPADEIPF
EEEEEECCCCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCH
CREQVVERHGFSKVRSIVAGGAERQQSVLNGLRAMEGTAGEYDVILIHDGVRPFVTEDIL
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHH
GRATATARENDGALVAVPAKDTVKVVEDGIITATPSRETLWLAQTPQAFRYGVIRAAHEV
HHHHCCCCCCCCCEEEECCCCHHHHHHCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHH
ADAERFLGTDDAMLVERLGRQVRIVLGDYRNVKITTPEDMVLAEAFIKEKLP
HHHHHHCCCCHHHHHHHCCCEEEEEEECCCEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA