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The map label for this gene is ispF

Identifier: 78221286

GI number: 78221286

Start: 73856

End: 74329

Strand: Reverse

Name: ispF

Synonym: Gmet_0059

Alternate gene names: 78221286

Gene position: 74329-73856 (Counterclockwise)

Preceding gene: 78221287

Following gene: 78221285

Centisome position: 1.86

GC content: 64.14

Gene sequence:

>474_bases
ATGAGAATCGGGCACGGCTACGACGTCCATTGCCTCGTGGAGGGGCGGAAACTGATTCTCGGAGGAGTTGATGTTCCCTA
CGAACGGGGGCTTTTGGGACACTCGGACGCCGACGTCCTCCTCCATGCCATCGCCGACGCCATCCTCGGGGCGTTGGCCC
TTGGGGACATCGGGAAGCACTTCCCCGACACCGACCCCCAGTTCAAGGGGGCCGACAGCCGCAAGCTCCTGCGCCACGTC
ATGGCCCTGGCCGGCCAGAAGGGATATGTCCTCGGCAACGTGGATGCCACCATCGTGGCCCAGCGCCCCAAGCTGGCTCC
CTTCATCCCGGAGATGCGCGCGAATCTCGCCGAAGACCTCATCGCTGAGATCGATCGGATCAACGTCAAGGCCACTACCA
CCGAGCAACTCGGCTTTGCCGGCCGAGGCGAGGGAATCGCCGCTTATGCGGTGGTCCTCATGGAGCGCAGGTAG

Upstream 100 bases:

>100_bases
GGCGACAGGTACGGATCGTTCTGGGAGACTACCGCAACGTTAAGATCACGACGCCGGAGGATATGGTTCTGGCAGAAGCG
TTCATCAAGGAGAAGCTGCC

Downstream 100 bases:

>100_bases
CGCCCCCCCTGAAATCACGTCCAAAGGATCGAGTCCATGTCAACCGAAACGCCCCACACCGCCAACTTCCTCCGCACCAT
CGTTCAGGAGGACCTGAAGG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRIGHGYDVHCLVEGRKLILGGVDVPYERGLLGHSDADVLLHAIADAILGALALGDIGKHFPDTDPQFKGADSRKLLRHV
MALAGQKGYVLGNVDATIVAQRPKLAPFIPEMRANLAEDLIAEIDRINVKATTTEQLGFAGRGEGIAAYAVVLMERR

Sequences:

>Translated_157_residues
MRIGHGYDVHCLVEGRKLILGGVDVPYERGLLGHSDADVLLHAIADAILGALALGDIGKHFPDTDPQFKGADSRKLLRHV
MALAGQKGYVLGNVDATIVAQRPKLAPFIPEMRANLAEDLIAEIDRINVKATTTEQLGFAGRGEGIAAYAVVLMERR
>Mature_157_residues
MRIGHGYDVHCLVEGRKLILGGVDVPYERGLLGHSDADVLLHAIADAILGALALGDIGKHFPDTDPQFKGADSRKLLRHV
MALAGQKGYVLGNVDATIVAQRPKLAPFIPEMRANLAEDLIAEIDRINVKATTTEQLGFAGRGEGIAAYAVVLMERR

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=64.9350649350649, Blast_Score=194, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_GEOMG (Q39ZL6)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383033.1
- ProteinModelPortal:   Q39ZL6
- SMR:   Q39ZL6
- STRING:   Q39ZL6
- GeneID:   3738547
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0059
- NMPDR:   fig|269799.3.peg.122
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   Q39ZL6
- ProtClustDB:   PRK00084
- BioCyc:   GMET269799:GMET_0059-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16890; Mature: 16890

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGHGYDVHCLVEGRKLILGGVDVPYERGLLGHSDADVLLHAIADAILGALALGDIGKH
CCCCCCCCEEEEEECCEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FPDTDPQFKGADSRKLLRHVMALAGQKGYVLGNVDATIVAQRPKLAPFIPEMRANLAEDL
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCCHHHHHHHHHHH
IAEIDRINVKATTTEQLGFAGRGEGIAAYAVVLMERR
HHHHHHCCCEEECHHHCCCCCCCCCHHHHHHEEECCC
>Mature Secondary Structure
MRIGHGYDVHCLVEGRKLILGGVDVPYERGLLGHSDADVLLHAIADAILGALALGDIGKH
CCCCCCCCEEEEEECCEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FPDTDPQFKGADSRKLLRHVMALAGQKGYVLGNVDATIVAQRPKLAPFIPEMRANLAEDL
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCCHHHHHHHHHHH
IAEIDRINVKATTTEQLGFAGRGEGIAAYAVVLMERR
HHHHHHCCCEEECHHHCCCCCCCCCHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA