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The map label for this gene is glnS [H]

Identifier: 78221285

GI number: 78221285

Start: 72158

End: 73819

Strand: Reverse

Name: glnS [H]

Synonym: Gmet_0058

Alternate gene names: 78221285

Gene position: 73819-72158 (Counterclockwise)

Preceding gene: 78221286

Following gene: 78221284

Centisome position: 1.85

GC content: 64.2

Gene sequence:

>1662_bases
ATGTCAACCGAAACGCCCCACACCGCCAACTTCCTCCGCACCATCGTTCAGGAGGACCTGAAGGGCGGCAAACGCGAAAC
CATCGTCACCCGCTTCCCCCCTGAGCCCAACGGCTATCTCCACATCGGCCACGCCAAGTCCATCTGCCTGAACTTCGGCC
TGGCAAAGGAGTTCGGGGGCCGCTGCCACCTCCGCTTCGACGACACGAACCCGGTAAAGGAAGAAACCGAGTATGTGGAA
TCCATCAAGGAAAGCGTGCGGTGGCTCGGCTTCTCATGGGGGACGCACCTCTACTACGCCTCCGAGTACTTCGAGCAGCT
CTACCAGTGGGCAGAGCACCTAATCCTCCAGGGGAAGGCCTACGTGGACGACCTGACGGCCGAGGAGATCCGGGCCCACC
GCGGCACCCTGACCGAGGCGGGGAAGGAGAGCCCCTGGCGCAACCGCTCCCCGGAGGAGAACCTGGATCTTTTCCGCCGG
ATGCGGGCCGGGGAGTTTCCCGACGGCGCCAAGGTGCTCCGGGCGAAGATAGACATGGCCTCCCCCAACATGAACCTCCG
GGACCCGGTCATGTACCGGATCATCCACGCCTCCCACCCCCACGTGGGTGACGCCTGGCGCATCTACCCCATGTACGACT
ACGCCCACGGCCAGTCCGACGCCATCGAGGGGATCACCCACTCCATCTGCACCCTGGAGTTCGAGGACCACAAGCCTCTC
TACGAGTGGTTCCTGGACAACCTGCCGGTGCCCAACCGCCCCCGCCAGTACGAGTTCGCGCGCCTGAACCTCACCTACGC
GGTCATGAGCAAGCGCAAGCTCCTGCAGTTGGTGAAGGACGGCGACGTCTCCGGCTGGGACGACCCGCGCATGCCTACCA
TCATGGGGATCCGGCGCCGTGGCTACACTCCCGAGGCGGTCAGAAACTTCTGCGAGACCATCGGCGTGGGGCGGAGCGAC
AGCTGGATCGACATGAGCATCCTGGAGGAGAGCGTTCGCCAGGACCTGAACGAGCGGGCACCCCGGGTCATGGCGGTGCT
GAAGCCCCTGCGGCTCGTCATAGAGGATTATCCGGAAGGGGAGGCGGAGGAGCTCACCATCCCGTACCACCCCCAGAAGC
CTGAGCTGGGGAGCCGGGTGGTCCCCTTCTGCAGCGAGCTCTTCATCGAGCAGGATGATTTTGCGGAAGTTCCCCCCAAG
GGATTCAAGCGCCTCTCCCCCGGGGAGGAGATCCGGCTGCGGGGGGCATACATCATTAAGTGCGTCGACGTGGAGAAGGA
TGCCGAGGGCGCCGTCACCGCGGTCCGCTGTACCTACGATCCCGAAACCCGCAGCGGTCTCCCAGGGTCCGAGCGGAAGG
TGAAGGGGGTCATCCACTGGGTGTCGGCCCGCCACGCCGAAACAGCGAAGGTGCGCCTCTACGACCGGCTCTTCACCGAC
CCTAACCCCTCCGGCGACGACTGGAAGGACCACCTGAATCCGGCATCCATGGAGATTCTGGCCGACTGCCGGCTGGAACC
CTCCCTTGCTACAGCCCAGGCCGAGGACCGCTTCCAGTTTGAACGGCAGGGATACTTCTGCGTTGACCTGAAGGATTCAC
GACCAGGGGCGCCGGTCTTCAACCGGACGGTGACCCTGCGGGATTCCTGGACCAAGAAATAA

Upstream 100 bases:

>100_bases
CGGCTTTGCCGGCCGAGGCGAGGGAATCGCCGCTTATGCGGTGGTCCTCATGGAGCGCAGGTAGCGCCCCCCCTGAAATC
ACGTCCAAAGGATCGAGTCC

Downstream 100 bases:

>100_bases
ACTCAAAGAGGTTAGTTGCATGTCTTTGCGCGTCTACAATACGCTTTCCGGAACCAAGGAAGAATTCACCCCCCTCGTGC
CGGGAAGGGTCTCCATGTAT

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MSTETPHTANFLRTIVQEDLKGGKRETIVTRFPPEPNGYLHIGHAKSICLNFGLAKEFGGRCHLRFDDTNPVKEETEYVE
SIKESVRWLGFSWGTHLYYASEYFEQLYQWAEHLILQGKAYVDDLTAEEIRAHRGTLTEAGKESPWRNRSPEENLDLFRR
MRAGEFPDGAKVLRAKIDMASPNMNLRDPVMYRIIHASHPHVGDAWRIYPMYDYAHGQSDAIEGITHSICTLEFEDHKPL
YEWFLDNLPVPNRPRQYEFARLNLTYAVMSKRKLLQLVKDGDVSGWDDPRMPTIMGIRRRGYTPEAVRNFCETIGVGRSD
SWIDMSILEESVRQDLNERAPRVMAVLKPLRLVIEDYPEGEAEELTIPYHPQKPELGSRVVPFCSELFIEQDDFAEVPPK
GFKRLSPGEEIRLRGAYIIKCVDVEKDAEGAVTAVRCTYDPETRSGLPGSERKVKGVIHWVSARHAETAKVRLYDRLFTD
PNPSGDDWKDHLNPASMEILADCRLEPSLATAQAEDRFQFERQGYFCVDLKDSRPGAPVFNRTVTLRDSWTKK

Sequences:

>Translated_553_residues
MSTETPHTANFLRTIVQEDLKGGKRETIVTRFPPEPNGYLHIGHAKSICLNFGLAKEFGGRCHLRFDDTNPVKEETEYVE
SIKESVRWLGFSWGTHLYYASEYFEQLYQWAEHLILQGKAYVDDLTAEEIRAHRGTLTEAGKESPWRNRSPEENLDLFRR
MRAGEFPDGAKVLRAKIDMASPNMNLRDPVMYRIIHASHPHVGDAWRIYPMYDYAHGQSDAIEGITHSICTLEFEDHKPL
YEWFLDNLPVPNRPRQYEFARLNLTYAVMSKRKLLQLVKDGDVSGWDDPRMPTIMGIRRRGYTPEAVRNFCETIGVGRSD
SWIDMSILEESVRQDLNERAPRVMAVLKPLRLVIEDYPEGEAEELTIPYHPQKPELGSRVVPFCSELFIEQDDFAEVPPK
GFKRLSPGEEIRLRGAYIIKCVDVEKDAEGAVTAVRCTYDPETRSGLPGSERKVKGVIHWVSARHAETAKVRLYDRLFTD
PNPSGDDWKDHLNPASMEILADCRLEPSLATAQAEDRFQFERQGYFCVDLKDSRPGAPVFNRTVTLRDSWTKK
>Mature_552_residues
STETPHTANFLRTIVQEDLKGGKRETIVTRFPPEPNGYLHIGHAKSICLNFGLAKEFGGRCHLRFDDTNPVKEETEYVES
IKESVRWLGFSWGTHLYYASEYFEQLYQWAEHLILQGKAYVDDLTAEEIRAHRGTLTEAGKESPWRNRSPEENLDLFRRM
RAGEFPDGAKVLRAKIDMASPNMNLRDPVMYRIIHASHPHVGDAWRIYPMYDYAHGQSDAIEGITHSICTLEFEDHKPLY
EWFLDNLPVPNRPRQYEFARLNLTYAVMSKRKLLQLVKDGDVSGWDDPRMPTIMGIRRRGYTPEAVRNFCETIGVGRSDS
WIDMSILEESVRQDLNERAPRVMAVLKPLRLVIEDYPEGEAEELTIPYHPQKPELGSRVVPFCSELFIEQDDFAEVPPKG
FKRLSPGEEIRLRGAYIIKCVDVEKDAEGAVTAVRCTYDPETRSGLPGSERKVKGVIHWVSARHAETAKVRLYDRLFTDP
NPSGDDWKDHLNPASMEILADCRLEPSLATAQAEDRFQFERQGYFCVDLKDSRPGAPVFNRTVTLRDSWTKK

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=550, Percent_Identity=45.4545454545455, Blast_Score=434, Evalue=1e-121,
Organism=Homo sapiens, GI62241042, Length=527, Percent_Identity=34.3453510436433, Blast_Score=280, Evalue=4e-75,
Organism=Escherichia coli, GI1786895, Length=553, Percent_Identity=59.6745027124774, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=308, Percent_Identity=25, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17543332, Length=553, Percent_Identity=41.5913200723327, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17511007, Length=522, Percent_Identity=34.0996168582375, Blast_Score=265, Evalue=6e-71,
Organism=Saccharomyces cerevisiae, GI6324742, Length=573, Percent_Identity=40.8376963350785, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321192, Length=539, Percent_Identity=35.2504638218924, Blast_Score=291, Evalue=3e-79,
Organism=Drosophila melanogaster, GI17864488, Length=556, Percent_Identity=40.8273381294964, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24649466, Length=527, Percent_Identity=33.0170777988615, Blast_Score=259, Evalue=3e-69,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 63568; Mature: 63436

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTETPHTANFLRTIVQEDLKGGKRETIVTRFPPEPNGYLHIGHAKSICLNFGLAKEFGG
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCHHHHHHHCCHHHHCCC
RCHLRFDDTNPVKEETEYVESIKESVRWLGFSWGTHLYYASEYFEQLYQWAEHLILQGKA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
YVDDLTAEEIRAHRGTLTEAGKESPWRNRSPEENLDLFRRMRAGEFPDGAKVLRAKIDMA
HHHHCCHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
SPNMNLRDPVMYRIIHASHPHVGDAWRIYPMYDYAHGQSDAIEGITHSICTLEFEDHKPL
CCCCCCCCCHHHHEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHEEEEEECCCCCH
YEWFLDNLPVPNRPRQYEFARLNLTYAVMSKRKLLQLVKDGDVSGWDDPRMPTIMGIRRR
HHHHHHCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHC
GYTPEAVRNFCETIGVGRSDSWIDMSILEESVRQDLNERAPRVMAVLKPLRLVIEDYPEG
CCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EAEELTIPYHPQKPELGSRVVPFCSELFIEQDDFAEVPPKGFKRLSPGEEIRLRGAYIIK
CCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEECCEEEEE
CVDVEKDAEGAVTAVRCTYDPETRSGLPGSERKVKGVIHWVSARHAETAKVRLYDRLFTD
EEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEEHHHHCCC
PNPSGDDWKDHLNPASMEILADCRLEPSLATAQAEDRFQFERQGYFCVDLKDSRPGAPVF
CCCCCCCHHHHCCHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCEEEEEECCCCCCCCCC
NRTVTLRDSWTKK
CCEEEECCCCCCC
>Mature Secondary Structure 
STETPHTANFLRTIVQEDLKGGKRETIVTRFPPEPNGYLHIGHAKSICLNFGLAKEFGG
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCHHHHHHHCCHHHHCCC
RCHLRFDDTNPVKEETEYVESIKESVRWLGFSWGTHLYYASEYFEQLYQWAEHLILQGKA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
YVDDLTAEEIRAHRGTLTEAGKESPWRNRSPEENLDLFRRMRAGEFPDGAKVLRAKIDMA
HHHHCCHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
SPNMNLRDPVMYRIIHASHPHVGDAWRIYPMYDYAHGQSDAIEGITHSICTLEFEDHKPL
CCCCCCCCCHHHHEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHEEEEEECCCCCH
YEWFLDNLPVPNRPRQYEFARLNLTYAVMSKRKLLQLVKDGDVSGWDDPRMPTIMGIRRR
HHHHHHCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHC
GYTPEAVRNFCETIGVGRSDSWIDMSILEESVRQDLNERAPRVMAVLKPLRLVIEDYPEG
CCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EAEELTIPYHPQKPELGSRVVPFCSELFIEQDDFAEVPPKGFKRLSPGEEIRLRGAYIIK
CCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEECCEEEEE
CVDVEKDAEGAVTAVRCTYDPETRSGLPGSERKVKGVIHWVSARHAETAKVRLYDRLFTD
EEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEEHHHHCCC
PNPSGDDWKDHLNPASMEILADCRLEPSLATAQAEDRFQFERQGYFCVDLKDSRPGAPVF
CCCCCCCHHHHCCHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCEEEEEECCCCCCCCCC
NRTVTLRDSWTKK
CCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]