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The map label for this gene is dnaN [H]

Identifier: 78221230

GI number: 78221230

Start: 1837

End: 2958

Strand: Direct

Name: dnaN [H]

Synonym: Gmet_0002

Alternate gene names: 78221230

Gene position: 1837-2958 (Clockwise)

Preceding gene: 78221229

Following gene: 78221231

Centisome position: 0.05

GC content: 60.25

Gene sequence:

>1122_bases
ATGGAATTCAAGATCGACAAGGAAACCTTCTCCCGGGCTCTCCAGAAGATCCAGGGAATCGTCGAAAAGAGGAACACCAT
GCCGATCCTCTCCAACGTCCTTATCGAGGCCCATGCCGATCGGATCGAGCTGACGGCCACGGACCTGGAAGTGGGAATGA
AGAGTTCCTACGAGACGACTGTGACCCGGGAGGGACGAATCACCGTTTCGGCCAAGAAGCTCTACGAGATCGTCAAGGAA
CTCCCCGAGGAAGCCATCTCTTTCGCCACCAAGGAGAACGACTGGGTGGAAATCCGCTGTGGCAAGGCCCATTTCAGCAT
CGTCGGCCTTTCCCCCGAGGAGTTTCCCTATTTCCCCAAGGTGAACGAAGAGAGCCTGATCCGGATCGAGGGGGCGCTCC
TGGCCGACATGATCGAGAAGACCGCCTACGCCATCTGCTTCGACGAGACCAAGTACAACCTGAACGGCACCTTCGTGAAG
GCCACCGTTGAAGATGATAAACCGATTCTCCGGATGGTGGCCACCGACGGCCACCGCCTCTCCATCGCCGAGAAGGAATT
CCACGGGGCCGTGGGGCCCGAGATGGCCAAGGGGGTCATCTTCCCCAAGAAGGGTATCTTCGAGCTCAAGAAGATCTGCG
AGGAGGCGACCACCGAAGAACTGGCCCTCGGCTTCATGGACAACAGCGCCGTCATCAAGAAGGGGAACACCGTGGTGGTG
ATGCGGCTCGTGGACGGTGAGTTCCCCGACTACACCCGCGTAGTCCCCGTGGCCAACGACAAGATCGTGACCGTGGCCCG
GGATCCGTTCCTCCACTCCCTGCGGCGGATGTCGATCCTCTCCAGCGAGAAGTTCAAGGGGATCCGGATGGAGATCAACG
GCGAGCAGATGATCATCTCCTCCAGCAACCCTGAACTGGGGGAAGCGTCGGAGGAACTGGACGCGGCCTACAGCGGCCCT
CCCCTTTCCATCCGGTTCAATGCCAAGTACCTCCTGGACGTCCTGGCGGTCCTCAACGAGAGCGAAGTGGCGCTGGTCCT
CCGGGACGAGCTGTCGCCGGCCATCGTGAAACCGGCCGCGGTCGACGGTTTCACCGCGGTGATCATGCCGATGCGGCTGT
AG

Upstream 100 bases:

>100_bases
GAGTTACCGGGTTATCCCCGGATCCACAGCCCCTATTGCTTCTACTAAGCTTTAAAAAAGCATAAACATACTAATCTGCA
TCTGTTGACAAGGAGGAGCG

Downstream 100 bases:

>100_bases
GAAACAGTAACCGGCAGGCACGGAAGGCGAAGGCCCCTCCCCGGGAGAGGCCTTTTGCCGTTCTTTCGGCTATAGAAACA
CCATGTTTGTGACGAAGATC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MEFKIDKETFSRALQKIQGIVEKRNTMPILSNVLIEAHADRIELTATDLEVGMKSSYETTVTREGRITVSAKKLYEIVKE
LPEEAISFATKENDWVEIRCGKAHFSIVGLSPEEFPYFPKVNEESLIRIEGALLADMIEKTAYAICFDETKYNLNGTFVK
ATVEDDKPILRMVATDGHRLSIAEKEFHGAVGPEMAKGVIFPKKGIFELKKICEEATTEELALGFMDNSAVIKKGNTVVV
MRLVDGEFPDYTRVVPVANDKIVTVARDPFLHSLRRMSILSSEKFKGIRMEINGEQMIISSSNPELGEASEELDAAYSGP
PLSIRFNAKYLLDVLAVLNESEVALVLRDELSPAIVKPAAVDGFTAVIMPMRL

Sequences:

>Translated_373_residues
MEFKIDKETFSRALQKIQGIVEKRNTMPILSNVLIEAHADRIELTATDLEVGMKSSYETTVTREGRITVSAKKLYEIVKE
LPEEAISFATKENDWVEIRCGKAHFSIVGLSPEEFPYFPKVNEESLIRIEGALLADMIEKTAYAICFDETKYNLNGTFVK
ATVEDDKPILRMVATDGHRLSIAEKEFHGAVGPEMAKGVIFPKKGIFELKKICEEATTEELALGFMDNSAVIKKGNTVVV
MRLVDGEFPDYTRVVPVANDKIVTVARDPFLHSLRRMSILSSEKFKGIRMEINGEQMIISSSNPELGEASEELDAAYSGP
PLSIRFNAKYLLDVLAVLNESEVALVLRDELSPAIVKPAAVDGFTAVIMPMRL
>Mature_373_residues
MEFKIDKETFSRALQKIQGIVEKRNTMPILSNVLIEAHADRIELTATDLEVGMKSSYETTVTREGRITVSAKKLYEIVKE
LPEEAISFATKENDWVEIRCGKAHFSIVGLSPEEFPYFPKVNEESLIRIEGALLADMIEKTAYAICFDETKYNLNGTFVK
ATVEDDKPILRMVATDGHRLSIAEKEFHGAVGPEMAKGVIFPKKGIFELKKICEEATTEELALGFMDNSAVIKKGNTVVV
MRLVDGEFPDYTRVVPVANDKIVTVARDPFLHSLRRMSILSSEKFKGIRMEINGEQMIISSSNPELGEASEELDAAYSGP
PLSIRFNAKYLLDVLAVLNESEVALVLRDELSPAIVKPAAVDGFTAVIMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=33.8666666666667, Blast_Score=211, Evalue=4e-56,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41527; Mature: 41527

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKIDKETFSRALQKIQGIVEKRNTMPILSNVLIEAHADRIELTATDLEVGMKSSYETT
CCEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCEE
VTREGRITVSAKKLYEIVKELPEEAISFATKENDWVEIRCGKAHFSIVGLSPEEFPYFPK
EEECCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEECCHHHCCCCCC
VNEESLIRIEGALLADMIEKTAYAICFDETKYNLNGTFVKATVEDDKPILRMVATDGHRL
CCCCCEEEEHHHHHHHHHHHHEEEEEEECCEECCCEEEEEEEECCCCCEEEEEECCCCEE
SIAEKEFHGAVGPEMAKGVIFPKKGIFELKKICEEATTEELALGFMDNSAVIKKGNTVVV
EEEHHHHCCCCCHHHHCCEECCCCCHHHHHHHHHHCCHHHHEEEEECCCEEEECCCEEEE
MRLVDGEFPDYTRVVPVANDKIVTVARDPFLHSLRRMSILSSEKFKGIRMEINGEQMIIS
EEEECCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEE
SSNPELGEASEELDAAYSGPPLSIRFNAKYLLDVLAVLNESEVALVLRDELSPAIVKPAA
CCCCCCCCCHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
VDGFTAVIMPMRL
CCCEEEEEEEECC
>Mature Secondary Structure
MEFKIDKETFSRALQKIQGIVEKRNTMPILSNVLIEAHADRIELTATDLEVGMKSSYETT
CCEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCEE
VTREGRITVSAKKLYEIVKELPEEAISFATKENDWVEIRCGKAHFSIVGLSPEEFPYFPK
EEECCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEECCHHHCCCCCC
VNEESLIRIEGALLADMIEKTAYAICFDETKYNLNGTFVKATVEDDKPILRMVATDGHRL
CCCCCEEEEHHHHHHHHHHHHEEEEEEECCEECCCEEEEEEEECCCCCEEEEEECCCCEE
SIAEKEFHGAVGPEMAKGVIFPKKGIFELKKICEEATTEELALGFMDNSAVIKKGNTVVV
EEEHHHHCCCCCHHHHCCEECCCCCHHHHHHHHHHCCHHHHEEEEECCCEEEECCCEEEE
MRLVDGEFPDYTRVVPVANDKIVTVARDPFLHSLRRMSILSSEKFKGIRMEINGEQMIIS
EEEECCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEE
SSNPELGEASEELDAAYSGPPLSIRFNAKYLLDVLAVLNESEVALVLRDELSPAIVKPAA
CCCCCCCCCHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
VDGFTAVIMPMRL
CCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]