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The map label for this gene is recF

Identifier: 78221231

GI number: 78221231

Start: 3041

End: 4138

Strand: Direct

Name: recF

Synonym: Gmet_0003

Alternate gene names: 78221231

Gene position: 3041-4138 (Clockwise)

Preceding gene: 78221230

Following gene: 78221232

Centisome position: 0.08

GC content: 64.94

Gene sequence:

>1098_bases
ATGTTTGTGACGAAGATCCAGATCAGTTCCTTCCGGAACATCGCCGCGGCGGAGATCCGCTTCGACCGGCGCTTCAATGT
CCTCCACGGCGCCAACGGCCAGGGGAAGACCTCGGTCCTGGAGGCCATCTATCTCCTGGGGACCATGAAGTCGTTTCGCC
TCGCCAAGACCCCTGATCTTGTCAGCTGGAACACCCCCCATGCGCTCCTGCGGGGATGGGCCGAGCGGGACGGGGTCGGC
AGGGAGATCGCCCTCTACCTGGGGAAGGAAGGCCGCAAAGCGAGGGTCGACCAGAAGCCGGTGACCCGGCTCGCCGATTT
CTTCGGCAACGTGAACGCCGTGGTCTTCTCCCCCGAGGAGATCGCCATGGCCCGCAGCGGCCCGGACCTCCGGCGCCGCT
ACCTGGACCGGGCCATCTTCAGCGGAGATCTGGGGTACCTGCTCCTCCACCACGAGTACCACCGGCTCCTCAAGCAGCGC
AACGCCCTGCTGAAGCGGGGAAGCCGGGAAGGGCTCGATATCTGGACCGGACAGCTGGCCGAAGCGGGTACCCGGCTCAT
GGTAAAGCGGATGGGATACCTGGCCGAGATAGAGCCGCTGGTGCAACGGTTCTACCGGGAGATTGCCGGGGGCGAGGAAG
AGGCGGGGCTTGCCTATCGTCCCCACCTCACTACCCCCGACTTGGTGAGTCGGGAGGGAACGGACGCGCTTCTGGCTCTC
TTCGGGGCCCATGAGGCGGAGGAACTCCGCCGGGGAACCACCGTGGTGGGCCCCCACCGGGACGACGTGGACTTCGTCCT
GAACGGCCGGGTGATCCGCACTCACGGCTCCCAGGGGCAACAGCGCAGCTTCGTCCTGGCCCTTAAGATGGCGGAGATCG
AGTACCTGGAGCGGCTCAACGACGCCCCGCCGGTGCTGCTCCTGGACGACATCTCGTCGGAGCTGGATCCGCAGCGCAAC
GCGAATCTCATGACCTTCCTCCGGGAAAAGCGGATGCAGGTTTTCATCACCACCACCGATGTTTCAACCCTCCGCCTGGC
GGGGATTGCAACCCACGCGTCCTTCCACGTTTCACGTGGAACGGTGACGCCTTTATAG

Upstream 100 bases:

>100_bases
ATGCCGATGCGGCTGTAGGAAACAGTAACCGGCAGGCACGGAAGGCGAAGGCCCCTCCCCGGGAGAGGCCTTTTGCCGTT
CTTTCGGCTATAGAAACACC

Downstream 100 bases:

>100_bases
AGGGAGAAGTATGGCTGACGAACTGAACAACGATTACGGAGCGGACAAGATCAAGGTCCTTGAGGGGCTCGAAGCTGTTC
GCAAGCGCCCCGCCATGTAC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MFVTKIQISSFRNIAAAEIRFDRRFNVLHGANGQGKTSVLEAIYLLGTMKSFRLAKTPDLVSWNTPHALLRGWAERDGVG
REIALYLGKEGRKARVDQKPVTRLADFFGNVNAVVFSPEEIAMARSGPDLRRRYLDRAIFSGDLGYLLLHHEYHRLLKQR
NALLKRGSREGLDIWTGQLAEAGTRLMVKRMGYLAEIEPLVQRFYREIAGGEEEAGLAYRPHLTTPDLVSREGTDALLAL
FGAHEAEELRRGTTVVGPHRDDVDFVLNGRVIRTHGSQGQQRSFVLALKMAEIEYLERLNDAPPVLLLDDISSELDPQRN
ANLMTFLREKRMQVFITTTDVSTLRLAGIATHASFHVSRGTVTPL

Sequences:

>Translated_365_residues
MFVTKIQISSFRNIAAAEIRFDRRFNVLHGANGQGKTSVLEAIYLLGTMKSFRLAKTPDLVSWNTPHALLRGWAERDGVG
REIALYLGKEGRKARVDQKPVTRLADFFGNVNAVVFSPEEIAMARSGPDLRRRYLDRAIFSGDLGYLLLHHEYHRLLKQR
NALLKRGSREGLDIWTGQLAEAGTRLMVKRMGYLAEIEPLVQRFYREIAGGEEEAGLAYRPHLTTPDLVSREGTDALLAL
FGAHEAEELRRGTTVVGPHRDDVDFVLNGRVIRTHGSQGQQRSFVLALKMAEIEYLERLNDAPPVLLLDDISSELDPQRN
ANLMTFLREKRMQVFITTTDVSTLRLAGIATHASFHVSRGTVTPL
>Mature_365_residues
MFVTKIQISSFRNIAAAEIRFDRRFNVLHGANGQGKTSVLEAIYLLGTMKSFRLAKTPDLVSWNTPHALLRGWAERDGVG
REIALYLGKEGRKARVDQKPVTRLADFFGNVNAVVFSPEEIAMARSGPDLRRRYLDRAIFSGDLGYLLLHHEYHRLLKQR
NALLKRGSREGLDIWTGQLAEAGTRLMVKRMGYLAEIEPLVQRFYREIAGGEEEAGLAYRPHLTTPDLVSREGTDALLAL
FGAHEAEELRRGTTVVGPHRDDVDFVLNGRVIRTHGSQGQQRSFVLALKMAEIEYLERLNDAPPVLLLDDISSELDPQRN
ANLMTFLREKRMQVFITTTDVSTLRLAGIATHASFHVSRGTVTPL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=378, Percent_Identity=28.5714285714286, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_GEOMG (Q39ZS1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_382978.1
- STRING:   Q39ZS1
- GeneID:   3739905
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0003
- NMPDR:   fig|269799.3.peg.67
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- PhylomeDB:   Q39ZS1
- ProtClustDB:   CLSK827576
- BioCyc:   GMET269799:GMET_0003-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41044; Mature: 41044

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVTKIQISSFRNIAAAEIRFDRRFNVLHGANGQGKTSVLEAIYLLGTMKSFRLAKTPDL
CEEEEEEHHHCCCHHHHHEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCCC
VSWNTPHALLRGWAERDGVGREIALYLGKEGRKARVDQKPVTRLADFFGNVNAVVFSPEE
CCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECHHH
IAMARSGPDLRRRYLDRAIFSGDLGYLLLHHEYHRLLKQRNALLKRGSREGLDIWTGQLA
HHHHCCCHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
EAGTRLMVKRMGYLAEIEPLVQRFYREIAGGEEEAGLAYRPHLTTPDLVSREGTDALLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHCCCCCHHHHHH
FGAHEAEELRRGTTVVGPHRDDVDFVLNGRVIRTHGSQGQQRSFVLALKMAEIEYLERLN
HCCCCHHHHHCCCEEECCCCCCCEEEECCEEEEECCCCCCCCEEEEEEHHHHHHHHHHCC
DAPPVLLLDDISSELDPQRNANLMTFLREKRMQVFITTTDVSTLRLAGIATHASFHVSRG
CCCCEEEEECCHHHCCCCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHEEECCEEEEECC
TVTPL
CCCCC
>Mature Secondary Structure
MFVTKIQISSFRNIAAAEIRFDRRFNVLHGANGQGKTSVLEAIYLLGTMKSFRLAKTPDL
CEEEEEEHHHCCCHHHHHEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCCC
VSWNTPHALLRGWAERDGVGREIALYLGKEGRKARVDQKPVTRLADFFGNVNAVVFSPEE
CCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECHHH
IAMARSGPDLRRRYLDRAIFSGDLGYLLLHHEYHRLLKQRNALLKRGSREGLDIWTGQLA
HHHHCCCHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
EAGTRLMVKRMGYLAEIEPLVQRFYREIAGGEEEAGLAYRPHLTTPDLVSREGTDALLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHCCCCCHHHHHH
FGAHEAEELRRGTTVVGPHRDDVDFVLNGRVIRTHGSQGQQRSFVLALKMAEIEYLERLN
HCCCCHHHHHCCCEEECCCCCCCEEEECCEEEEECCCCCCCCEEEEEEHHHHHHHHHHCC
DAPPVLLLDDISSELDPQRNANLMTFLREKRMQVFITTTDVSTLRLAGIATHASFHVSRG
CCCCEEEEECCHHHCCCCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHEEECCEEEEECC
TVTPL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA