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The map label for this gene is hemL

Identifier: 78189921

GI number: 78189921

Start: 2500292

End: 2501587

Strand: Reverse

Name: hemL

Synonym: Cag_1968

Alternate gene names: 78189921

Gene position: 2501587-2500292 (Counterclockwise)

Preceding gene: 78189922

Following gene: 78189920

Centisome position: 97.26

GC content: 48.53

Gene sequence:

>1296_bases
ATGCCTCAAATCACCCGTTCTATTGAACTCTTTGAAAAAGCAAAGAAGTTTATCCCCGGTGGCGTTAACTCACCAGTACG
CGCCTTTAAATCTGTTGGCGGCACACCAATTTACATGGCAAAAGGCTCTGGCGCTTACATGACCGACGTGGACGGCAACA
CCTACCTCGATTACGTTGGTTCATGGGGACCATTCATTCTCGGCAGTATGCACCCACGCATTACCGCTGCACTTGAGTAC
ACGCTAAAAAATATTGGCACCAGCTTTGGTACACCAATTGAGATGGAAATTGAAATTGCTGAACTGCTTTGCCAAATTGT
GCCTTCACTTGAAATGGTGCGTATGGTAAACAGCGGCACCGAAGCCACCATGTCAGCCGTGCGCCTTGCACGCGGTTACA
CCGGTCGCGATAAAATCATCAAATTTGAAGGTTGCTACCACGGGCACGGCGATAGCTTCCTCATTAAAGCAGGTTCAGGC
GCTCTTACGCTTGGTGCTCCCGATAGCCCTGGCGTTACCAAAGGCACAGCTCAGGACACTCTGAACGCAACCTATAACGA
CATCGAATCAGTAAAGTTGCTTGTTCAAGAGAACAAAGGCAACGTTGCTGCAATTATTATTGAACCTGTTGCTGGTAACA
CCGGTGTTATTCCAGCCCAACCCGGATTCCTTGCTGCACTCCGTCAGCTTTGCGACGAAGAAGGCATTGTGCTGATTTTT
GACGAAGTGATGTGCGGCTTCCGCGTAGCACTTGGCGGCGCACAAAGCCTTTATGGCGTTACCCCCGACCTTACCACAAT
GGGCAAAATTATTGGCGGTGGTCTGCCTGTTGGTGCATTTGGCGGCAAACGCAAGCTCATGGAGCGCGTTGCACCACTTG
GCGACGTTTACCAAGCTGGTACGCTTTCAGGTAACCCGCTGGCACTGACCGCTGGTCTTGAAACCTTGAAAATTCTCATG
GATGAGAATCCATATCCAGAGCTTGAAAGAAAAGCTGTTATTCTTGAAGAGGGCTTTAAAGCAAACCTTGCAAAACTTGG
CTTGAACTATGTTCAGAACCGTGTTGGTTCCATGTCGTGCCTCTTCTTTACCGAAACGCCTGTTGTGAACTACACAACCG
CTATTACGGCTGATACCAAGAAGCACGCCAAATACTTCCACTCATTGCTCGATCAAGGCATTTACACGGCTCCATCGCAG
TTTGAAGCAATGTTCATCAGCTCAGTAATGACCGACGAAGATTTGGATAAAACCATCAAAGCAAACTACAACGCTTTGGT
TGCTTCACAGCAATAA

Upstream 100 bases:

>100_bases
CTAATGTTACAACGGTTTTATTATCTTCGGCAGTGTTCTATCTTCACCGCATAATTTTTTGCATAAACCATTCTATTCAA
CTAAACCGCATATTCTAAAC

Downstream 100 bases:

>100_bases
GCTGCCGAGTTCCATAGCTTGATTGCTTTAGGCAAAAGGGGGGAGGTGTTGAGCCTCCCTTTTTTGTTGGGGGGGAGAGG
AGAAAAGGAGGGCGGACATT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPQITRSIELFEKAKKFIPGGVNSPVRAFKSVGGTPIYMAKGSGAYMTDVDGNTYLDYVGSWGPFILGSMHPRITAALEY
TLKNIGTSFGTPIEMEIEIAELLCQIVPSLEMVRMVNSGTEATMSAVRLARGYTGRDKIIKFEGCYHGHGDSFLIKAGSG
ALTLGAPDSPGVTKGTAQDTLNATYNDIESVKLLVQENKGNVAAIIIEPVAGNTGVIPAQPGFLAALRQLCDEEGIVLIF
DEVMCGFRVALGGAQSLYGVTPDLTTMGKIIGGGLPVGAFGGKRKLMERVAPLGDVYQAGTLSGNPLALTAGLETLKILM
DENPYPELERKAVILEEGFKANLAKLGLNYVQNRVGSMSCLFFTETPVVNYTTAITADTKKHAKYFHSLLDQGIYTAPSQ
FEAMFISSVMTDEDLDKTIKANYNALVASQQ

Sequences:

>Translated_431_residues
MPQITRSIELFEKAKKFIPGGVNSPVRAFKSVGGTPIYMAKGSGAYMTDVDGNTYLDYVGSWGPFILGSMHPRITAALEY
TLKNIGTSFGTPIEMEIEIAELLCQIVPSLEMVRMVNSGTEATMSAVRLARGYTGRDKIIKFEGCYHGHGDSFLIKAGSG
ALTLGAPDSPGVTKGTAQDTLNATYNDIESVKLLVQENKGNVAAIIIEPVAGNTGVIPAQPGFLAALRQLCDEEGIVLIF
DEVMCGFRVALGGAQSLYGVTPDLTTMGKIIGGGLPVGAFGGKRKLMERVAPLGDVYQAGTLSGNPLALTAGLETLKILM
DENPYPELERKAVILEEGFKANLAKLGLNYVQNRVGSMSCLFFTETPVVNYTTAITADTKKHAKYFHSLLDQGIYTAPSQ
FEAMFISSVMTDEDLDKTIKANYNALVASQQ
>Mature_430_residues
PQITRSIELFEKAKKFIPGGVNSPVRAFKSVGGTPIYMAKGSGAYMTDVDGNTYLDYVGSWGPFILGSMHPRITAALEYT
LKNIGTSFGTPIEMEIEIAELLCQIVPSLEMVRMVNSGTEATMSAVRLARGYTGRDKIIKFEGCYHGHGDSFLIKAGSGA
LTLGAPDSPGVTKGTAQDTLNATYNDIESVKLLVQENKGNVAAIIIEPVAGNTGVIPAQPGFLAALRQLCDEEGIVLIFD
EVMCGFRVALGGAQSLYGVTPDLTTMGKIIGGGLPVGAFGGKRKLMERVAPLGDVYQAGTLSGNPLALTAGLETLKILMD
ENPYPELERKAVILEEGFKANLAKLGLNYVQNRVGSMSCLFFTETPVVNYTTAITADTKKHAKYFHSLLDQGIYTAPSQF
EAMFISSVMTDEDLDKTIKANYNALVASQQ

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=315, Percent_Identity=29.2063492063492, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI13994255, Length=324, Percent_Identity=28.7037037037037, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI37574042, Length=332, Percent_Identity=28.9156626506024, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI24119277, Length=305, Percent_Identity=28.1967213114754, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI226442705, Length=333, Percent_Identity=29.4294294294294, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI226442709, Length=301, Percent_Identity=28.9036544850498, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI284507298, Length=232, Percent_Identity=30.6034482758621, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1786349, Length=419, Percent_Identity=54.4152744630072, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI1789016, Length=326, Percent_Identity=34.6625766871166, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1788044, Length=292, Percent_Identity=33.5616438356164, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789759, Length=335, Percent_Identity=31.044776119403, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1787560, Length=292, Percent_Identity=32.8767123287671, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI145693181, Length=326, Percent_Identity=29.7546012269939, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786991, Length=299, Percent_Identity=28.0936454849498, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71992977, Length=347, Percent_Identity=28.8184438040346, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=331, Percent_Identity=27.7945619335347, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32564660, Length=351, Percent_Identity=28.7749287749288, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI25144274, Length=140, Percent_Identity=33.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323470, Length=413, Percent_Identity=26.634382566586, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=330, Percent_Identity=24.8484848484848, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21357415, Length=299, Percent_Identity=27.0903010033445, Blast_Score=95, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_CHLCH (Q3AP59)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_380259.1
- ProteinModelPortal:   Q3AP59
- SMR:   Q3AP59
- STRING:   Q3AP59
- GeneID:   3747830
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1968
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   NANIPFF
- ProtClustDB:   PRK00062
- BioCyc:   CCHL340177:CAG_1968-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46155; Mature: 46024

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQITRSIELFEKAKKFIPGGVNSPVRAFKSVGGTPIYMAKGSGAYMTDVDGNTYLDYVG
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCEEEEECC
SWGPFILGSMHPRITAALEYTLKNIGTSFGTPIEMEIEIAELLCQIVPSLEMVRMVNSGT
CCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHCCCH
EATMSAVRLARGYTGRDKIIKFEGCYHGHGDSFLIKAGSGALTLGAPDSPGVTKGTAQDT
HHHHHHHHHHCCCCCCCCEEEEECEECCCCCEEEEEECCCEEEECCCCCCCCCCCCHHHH
LNATYNDIESVKLLVQENKGNVAAIIIEPVAGNTGVIPAQPGFLAALRQLCDEEGIVLIF
HHCHHHHHHHEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCEEEEE
DEVMCGFRVALGGAQSLYGVTPDLTTMGKIIGGGLPVGAFGGKRKLMERVAPLGDVYQAG
HHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHCC
TLSGNPLALTAGLETLKILMDENPYPELERKAVILEEGFKANLAKLGLNYVQNRVGSMSC
CCCCCCEEEECCHHHHHHEECCCCCCCHHHCEEEECCCCHHHHHHHHHHHHHHHCCCEEE
LFFTETPVVNYTTAITADTKKHAKYFHSLLDQGIYTAPSQFEAMFISSVMTDEDLDKTIK
EEEECCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
ANYNALVASQQ
CCCCEEEECCC
>Mature Secondary Structure 
PQITRSIELFEKAKKFIPGGVNSPVRAFKSVGGTPIYMAKGSGAYMTDVDGNTYLDYVG
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCEEEEECC
SWGPFILGSMHPRITAALEYTLKNIGTSFGTPIEMEIEIAELLCQIVPSLEMVRMVNSGT
CCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHCCCH
EATMSAVRLARGYTGRDKIIKFEGCYHGHGDSFLIKAGSGALTLGAPDSPGVTKGTAQDT
HHHHHHHHHHCCCCCCCCEEEEECEECCCCCEEEEEECCCEEEECCCCCCCCCCCCHHHH
LNATYNDIESVKLLVQENKGNVAAIIIEPVAGNTGVIPAQPGFLAALRQLCDEEGIVLIF
HHCHHHHHHHEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCEEEEE
DEVMCGFRVALGGAQSLYGVTPDLTTMGKIIGGGLPVGAFGGKRKLMERVAPLGDVYQAG
HHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHCC
TLSGNPLALTAGLETLKILMDENPYPELERKAVILEEGFKANLAKLGLNYVQNRVGSMSC
CCCCCCEEEECCHHHHHHEECCCCCCCHHHCEEEECCCCHHHHHHHHHHHHHHHCCCEEE
LFFTETPVVNYTTAITADTKKHAKYFHSLLDQGIYTAPSQFEAMFISSVMTDEDLDKTIK
EEEECCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
ANYNALVASQQ
CCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA