Click here to switch to the map view.

The map label for this gene is leuB

Identifier: 78189856

GI number: 78189856

Start: 2420036

End: 2421094

Strand: Reverse

Name: leuB

Synonym: Cag_1901

Alternate gene names: 78189856

Gene position: 2421094-2420036 (Counterclockwise)

Preceding gene: 78189857

Following gene: 78189855

Centisome position: 94.13

GC content: 45.8

Gene sequence:

>1059_bases
ATGAACTATAAAATTGTCTCTATTCCGGGTGATGGTATAGGTACCGAAGTTGTTGCTGGCGCTGTTGCTGTATTACGTCA
ACTTGAAAAAAAATATGGCTTTACCGTTGAGATTGAAGAGCATCTTTTTGGTGGCGCTTCTTACGATGTGCATGGTGAAA
TGTTAACCGATGCTACGCTTGAAGCCTGCAAAAATTGCGATGCCGTGCTGCTTGGAGCTGTTGGTGGTCCAAAATGGGAA
AACCTTCCCCACGAGCACAAGCCTGAAGCTGCGTTGCTTAAAATCCGCAAAGAGCTTGGCTTGTTTGCCAACCTTCGCCC
AGCAAAAGTGTATGATGCCTTAGTTGATGCTTCATCACTAAAAGCGGATGTTGTGCGTGGCACCGATTTTGTGGTTTTTC
GTGAGCTAACGGGTGGTATTTACTTCGGTCAACCTCGTGGCTACGATGAGAACAAAGGCTGGAACACCATGGTTTATGAA
AAGTATGAGGTTGAGCGTATTGCTCGCCTTGCTTTTGAAGCGGCTCGCCAACGCCAAGGGCGCGTTATGTCTATTGATAA
GGCAAACGTCCTTGAAGTATCACAATTGTGGCGGAACGTTGTTCACGCTGTACACGCCGATTACCAAGATGTTGAATTGA
GTGATATGTATGTGGATAATGCTGCAATGCAAATTGTACGTAATCCAAAACAGTTTGACGTTATTGTTACTGGCAACCTT
TTTGGTGATATTCTGAGCGATATTTCAGGCATGATTACTGGTAGCCTTGGCATGTTGCCTTCGGCAAGCATTGGTTCTAA
GCACGCACTATATGAGCCAATTCACGGCAGTGCCCCCGATATTGCAGGACAAAACAAAGCAAACCCCATTGCAACCATTG
CTTCGGTAGCAATGATGTTTGAACACAGCTTTAAGCGTACCGATATTGCTCGTGATATTGAACAAGCCATTGAAGCTGCC
CTTGCTACCGGTGTAAGAACGGCAGACATTGCAGCAGCCGGCGATACAGCAGTTTCAACCACAGCAATGACTGAAGCCAT
TATCAGCCAACTGAAGTAA

Upstream 100 bases:

>100_bases
GTTAAGAGAGAGCAACAGCAACCACGCTATCGAAAAAGTTGGTGCAAAGCTTCGCAACATGATGAGCTGGCTTATTAAAA
AATAATCTCGGAAAACCTCC

Downstream 100 bases:

>100_bases
GTAACAGTGCTGATTGAATTTATATCCAACACCTCAGTATTTTTTACGGCTGAGGTGTTTTTTCGCTTATTCGTAATGCC
CATAACCTTTCATTTTTGAA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNYKIVSIPGDGIGTEVVAGAVAVLRQLEKKYGFTVEIEEHLFGGASYDVHGEMLTDATLEACKNCDAVLLGAVGGPKWE
NLPHEHKPEAALLKIRKELGLFANLRPAKVYDALVDASSLKADVVRGTDFVVFRELTGGIYFGQPRGYDENKGWNTMVYE
KYEVERIARLAFEAARQRQGRVMSIDKANVLEVSQLWRNVVHAVHADYQDVELSDMYVDNAAMQIVRNPKQFDVIVTGNL
FGDILSDISGMITGSLGMLPSASIGSKHALYEPIHGSAPDIAGQNKANPIATIASVAMMFEHSFKRTDIARDIEQAIEAA
LATGVRTADIAAAGDTAVSTTAMTEAIISQLK

Sequences:

>Translated_352_residues
MNYKIVSIPGDGIGTEVVAGAVAVLRQLEKKYGFTVEIEEHLFGGASYDVHGEMLTDATLEACKNCDAVLLGAVGGPKWE
NLPHEHKPEAALLKIRKELGLFANLRPAKVYDALVDASSLKADVVRGTDFVVFRELTGGIYFGQPRGYDENKGWNTMVYE
KYEVERIARLAFEAARQRQGRVMSIDKANVLEVSQLWRNVVHAVHADYQDVELSDMYVDNAAMQIVRNPKQFDVIVTGNL
FGDILSDISGMITGSLGMLPSASIGSKHALYEPIHGSAPDIAGQNKANPIATIASVAMMFEHSFKRTDIARDIEQAIEAA
LATGVRTADIAAAGDTAVSTTAMTEAIISQLK
>Mature_352_residues
MNYKIVSIPGDGIGTEVVAGAVAVLRQLEKKYGFTVEIEEHLFGGASYDVHGEMLTDATLEACKNCDAVLLGAVGGPKWE
NLPHEHKPEAALLKIRKELGLFANLRPAKVYDALVDASSLKADVVRGTDFVVFRELTGGIYFGQPRGYDENKGWNTMVYE
KYEVERIARLAFEAARQRQGRVMSIDKANVLEVSQLWRNVVHAVHADYQDVELSDMYVDNAAMQIVRNPKQFDVIVTGNL
FGDILSDISGMITGSLGMLPSASIGSKHALYEPIHGSAPDIAGQNKANPIATIASVAMMFEHSFKRTDIARDIEQAIEAA
LATGVRTADIAAAGDTAVSTTAMTEAIISQLK

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=323, Percent_Identity=32.5077399380805, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI28178821, Length=314, Percent_Identity=28.0254777070064, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI28178816, Length=314, Percent_Identity=28.0254777070064, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI4758582, Length=342, Percent_Identity=29.8245614035088, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178838, Length=326, Percent_Identity=30.0613496932515, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI28178819, Length=142, Percent_Identity=33.8028169014084, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI87081683, Length=353, Percent_Identity=52.1246458923513, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1788101, Length=361, Percent_Identity=34.9030470914127, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1787381, Length=402, Percent_Identity=26.6169154228856, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71986051, Length=355, Percent_Identity=31.2676056338028, Blast_Score=147, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17550882, Length=353, Percent_Identity=26.9121813031161, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=301, Percent_Identity=29.9003322259136, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25144293, Length=311, Percent_Identity=28.9389067524116, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=44.9035812672176, Blast_Score=282, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6322097, Length=361, Percent_Identity=34.0720221606648, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6324709, Length=356, Percent_Identity=33.9887640449438, Blast_Score=156, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=357, Percent_Identity=30.5322128851541, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24643270, Length=318, Percent_Identity=31.1320754716981, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24643268, Length=325, Percent_Identity=29.8461538461538, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI161078637, Length=354, Percent_Identity=29.0960451977401, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078635, Length=354, Percent_Identity=29.0960451977401, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078639, Length=261, Percent_Identity=32.5670498084291, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078633, Length=354, Percent_Identity=29.0960451977401, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24650122, Length=354, Percent_Identity=29.0960451977401, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24661184, Length=358, Percent_Identity=26.536312849162, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281362242, Length=347, Percent_Identity=28.2420749279539, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648872, Length=347, Percent_Identity=28.2420749279539, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI20130355, Length=350, Percent_Identity=23.7142857142857, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_CHLCH (Q3APC4)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_380194.1
- HSSP:   Q9WZ26
- ProteinModelPortal:   Q3APC4
- SMR:   Q3APC4
- STRING:   Q3APC4
- GeneID:   3747646
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1901
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   CCHL340177:CAG_1901-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38185; Mature: 38185

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 96-96 BINDING 106-106 BINDING 134-134 BINDING 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKIVSIPGDGIGTEVVAGAVAVLRQLEKKYGFTVEIEEHLFGGASYDVHGEMLTDATL
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCCCHHHHHHHH
EACKNCDAVLLGAVGGPKWENLPHEHKPEAALLKIRKELGLFANLRPAKVYDALVDASSL
HHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KADVVRGTDFVVFRELTGGIYFGQPRGYDENKGWNTMVYEKYEVERIARLAFEAARQRQG
HHHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCC
RVMSIDKANVLEVSQLWRNVVHAVHADYQDVELSDMYVDNAAMQIVRNPKQFDVIVTGNL
CEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHH
FGDILSDISGMITGSLGMLPSASIGSKHALYEPIHGSAPDIAGQNKANPIATIASVAMMF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
EHSFKRTDIARDIEQAIEAALATGVRTADIAAAGDTAVSTTAMTEAIISQLK
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYKIVSIPGDGIGTEVVAGAVAVLRQLEKKYGFTVEIEEHLFGGASYDVHGEMLTDATL
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCCCHHHHHHHH
EACKNCDAVLLGAVGGPKWENLPHEHKPEAALLKIRKELGLFANLRPAKVYDALVDASSL
HHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KADVVRGTDFVVFRELTGGIYFGQPRGYDENKGWNTMVYEKYEVERIARLAFEAARQRQG
HHHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCC
RVMSIDKANVLEVSQLWRNVVHAVHADYQDVELSDMYVDNAAMQIVRNPKQFDVIVTGNL
CEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHH
FGDILSDISGMITGSLGMLPSASIGSKHALYEPIHGSAPDIAGQNKANPIATIASVAMMF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
EHSFKRTDIARDIEQAIEAALATGVRTADIAAAGDTAVSTTAMTEAIISQLK
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA