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The map label for this gene is leuC [H]

Identifier: 78189855

GI number: 78189855

Start: 2418637

End: 2419932

Strand: Reverse

Name: leuC [H]

Synonym: Cag_1900

Alternate gene names: 78189855

Gene position: 2419932-2418637 (Counterclockwise)

Preceding gene: 78189856

Following gene: 78189854

Centisome position: 94.08

GC content: 47.22

Gene sequence:

>1296_bases
ATGGCACAAACAATAACCCAGAAAATTTTTGCAAAATCAGCAAAACGCCCTTTTGTTGATCCCGGCGAAAGTGTTTGGCT
TAATGTGGATGTTCTCCTCACACATGACGTGTGCGGACCGCCCACGATTGATATTTTTAAAGAGAAATTTGGCTCCAACG
CTAAAGTGTGGGATCCCGAAAAAGTTATTATTCTGCCTGATCACTACATCTTTACTGCCAACGAACATGCGCACCGCAAC
ATTGATTTGCTGCGCCAATTTGCTAAAGAGCAAGGCTTACCTCATTACTACGATGTTGGCACCGATCGTTATAAAGGTGT
GTGCCATGTAGCACTTGCTGAAGAGGGCTTTAACCTTCCCGGTACCGTGCTTTTTGGTACCGACTCACACACCTGCACCT
CTGGCGCTTTTGGCATGTTTGGTACCGGCATTGGCAATACCGATGCGGCATTTATTCTTGGTACCGGCAAATTGTGGGAA
AAAGTACCCGAATCAATGAAGTTCACCTTTGAGGGTGAAATGCCAGCCTATTTGACAGCTAAAGATCTGATTTTGCAGAT
TCTTGGCGACATCACCACCGATGGTGCAACCTATCGCGCTATGGAGTTTGATGGCGAAGCTATTTTCTCTCTGCCAATGG
AAGAGCGCATGACGCTTACCAACATGGCAATTGAAGCGGGTGGCATGAATGGCATTATTGCAGCCGATAACATTGCAGAA
GAGTATGTAAAGGCACGTACCAAAAAGCCTTACGAGATTTTCCAAAGCGATCCTGACGCAAAGTACCATAGCACCTATCG
CTATAACGTGCGTGATTTGGAGCCTGTAGTAGCTCAACCGCATAGCCCCGATAACCGTGCAACCGTGCGTAGCGTAGCTG
GCACAAAAATCACCAAATCGTACATTGGCTCATGCACGGGTGGCAAGCTAAGCGACTTTATGATGGCAGCTAAAATCCTA
AAAGGGCAGAAAGTTACCGTTACCACAACTATTGTTCCTGCAACTACTTTAGTAGCTCGTAGCCTTGAAACGGAGCAATA
CGATGGCAAAAGCTTAAAGCAAATTTTTGAAGAAGCTGGCTGCAACGTTGCTTTACCATCGTGCGCTGCCTGTCTTGGCG
GTCCAGCTGACACCGTTGGTCGTTCGGTGGATAATGACCTTGTGGTTTCAACAACGAACCGCAACTTCCCTGGACGCATG
GGTAGCAAACATGCAGGCGTTTATCTTGCTTCACCACTTACGGCAGCGGCATCAGCAATTACCGGCAAACTTACCGATCC
GAGAGATTTTCTCTGA

Upstream 100 bases:

>100_bases
ACAGTGCTGATTGAATTTATATCCAACACCTCAGTATTTTTTACGGCTGAGGTGTTTTTTCGCTTATTCGTAATGCCCAT
AACCTTTCATTTTTGAACGT

Downstream 100 bases:

>100_bases
TTGTAGGAACTAAGAGAATCACACTATTATGGAAACAATCATTCAGGGAAAAGCCTACGTGTTAGGCAAGAATATTGATA
CCGATCAAATTATTCCCGCT

Product: 3-isopropylmalate dehydratase

Products: NA

Alternate protein names: (R)-2-methylmalate dehydratase; (R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAQTITQKIFAKSAKRPFVDPGESVWLNVDVLLTHDVCGPPTIDIFKEKFGSNAKVWDPEKVIILPDHYIFTANEHAHRN
IDLLRQFAKEQGLPHYYDVGTDRYKGVCHVALAEEGFNLPGTVLFGTDSHTCTSGAFGMFGTGIGNTDAAFILGTGKLWE
KVPESMKFTFEGEMPAYLTAKDLILQILGDITTDGATYRAMEFDGEAIFSLPMEERMTLTNMAIEAGGMNGIIAADNIAE
EYVKARTKKPYEIFQSDPDAKYHSTYRYNVRDLEPVVAQPHSPDNRATVRSVAGTKITKSYIGSCTGGKLSDFMMAAKIL
KGQKVTVTTTIVPATTLVARSLETEQYDGKSLKQIFEEAGCNVALPSCAACLGGPADTVGRSVDNDLVVSTTNRNFPGRM
GSKHAGVYLASPLTAAASAITGKLTDPRDFL

Sequences:

>Translated_431_residues
MAQTITQKIFAKSAKRPFVDPGESVWLNVDVLLTHDVCGPPTIDIFKEKFGSNAKVWDPEKVIILPDHYIFTANEHAHRN
IDLLRQFAKEQGLPHYYDVGTDRYKGVCHVALAEEGFNLPGTVLFGTDSHTCTSGAFGMFGTGIGNTDAAFILGTGKLWE
KVPESMKFTFEGEMPAYLTAKDLILQILGDITTDGATYRAMEFDGEAIFSLPMEERMTLTNMAIEAGGMNGIIAADNIAE
EYVKARTKKPYEIFQSDPDAKYHSTYRYNVRDLEPVVAQPHSPDNRATVRSVAGTKITKSYIGSCTGGKLSDFMMAAKIL
KGQKVTVTTTIVPATTLVARSLETEQYDGKSLKQIFEEAGCNVALPSCAACLGGPADTVGRSVDNDLVVSTTNRNFPGRM
GSKHAGVYLASPLTAAASAITGKLTDPRDFL
>Mature_430_residues
AQTITQKIFAKSAKRPFVDPGESVWLNVDVLLTHDVCGPPTIDIFKEKFGSNAKVWDPEKVIILPDHYIFTANEHAHRNI
DLLRQFAKEQGLPHYYDVGTDRYKGVCHVALAEEGFNLPGTVLFGTDSHTCTSGAFGMFGTGIGNTDAAFILGTGKLWEK
VPESMKFTFEGEMPAYLTAKDLILQILGDITTDGATYRAMEFDGEAIFSLPMEERMTLTNMAIEAGGMNGIIAADNIAEE
YVKARTKKPYEIFQSDPDAKYHSTYRYNVRDLEPVVAQPHSPDNRATVRSVAGTKITKSYIGSCTGGKLSDFMMAAKILK
GQKVTVTTTIVPATTLVARSLETEQYDGKSLKQIFEEAGCNVALPSCAACLGGPADTVGRSVDNDLVVSTTNRNFPGRMG
SKHAGVYLASPLTAAASAITGKLTDPRDFL

Specific function: Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate, a step involved

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=342, Percent_Identity=26.0233918128655, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786259, Length=481, Percent_Identity=32.2245322245322, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI2367097, Length=372, Percent_Identity=29.5698924731183, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787531, Length=394, Percent_Identity=24.1116751269036, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=341, Percent_Identity=26.6862170087977, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32564738, Length=341, Percent_Identity=26.6862170087977, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17568399, Length=386, Percent_Identity=25.9067357512953, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25149342, Length=289, Percent_Identity=25.9515570934256, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=486, Percent_Identity=30.4526748971193, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6320440, Length=459, Percent_Identity=30.0653594771242, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323335, Length=343, Percent_Identity=27.6967930029155, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=370, Percent_Identity=25.9459459459459, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161076999, Length=342, Percent_Identity=24.8538011695906, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281365315, Length=342, Percent_Identity=24.8538011695906, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17864292, Length=342, Percent_Identity=24.8538011695906, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28571643, Length=341, Percent_Identity=25.8064516129032, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI17137564, Length=369, Percent_Identity=26.2872628726287, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24645686, Length=371, Percent_Identity=25.0673854447439, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33; =4.2.1.35; =4.2.1.31 [H]

Molecular weight: Translated: 46836; Mature: 46705

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTITQKIFAKSAKRPFVDPGESVWLNVDVLLTHDVCGPPTIDIFKEKFGSNAKVWDPE
CCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCEECCC
KVIILPDHYIFTANEHAHRNIDLLRQFAKEQGLPHYYDVGTDRYKGVCHVALAEEGFNLP
EEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCCC
GTVLFGTDSHTCTSGAFGMFGTGIGNTDAAFILGTGKLWEKVPESMKFTFEGEMPAYLTA
CEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHCCHHEEEEECCCCCCEEHH
KDLILQILGDITTDGATYRAMEFDGEAIFSLPMEERMTLTNMAIEAGGMNGIIAADNIAE
HHHHHHHHHHCCCCCCEEEEEEECCCEEEECCHHHHHHHHHHEEECCCCCCEEECHHHHH
EYVKARTKKPYEIFQSDPDAKYHSTYRYNVRDLEPVVAQPHSPDNRATVRSVAGTKITKS
HHHHHHCCCCHHHHCCCCCCCCCCEEEECCCCCCHHHCCCCCCCCCHHHHHHHCCHHHHH
YIGSCTGGKLSDFMMAAKILKGQKVTVTTTIVPATTLVARSLETEQYDGKSLKQIFEEAG
HHCCCCCCHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
CNVALPSCAACLGGPADTVGRSVDNDLVVSTTNRNFPGRMGSKHAGVYLASPLTAAASAI
CCEECCHHHHHHCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHH
TGKLTDPRDFL
CCCCCCCCCCC
>Mature Secondary Structure 
AQTITQKIFAKSAKRPFVDPGESVWLNVDVLLTHDVCGPPTIDIFKEKFGSNAKVWDPE
CHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCEECCC
KVIILPDHYIFTANEHAHRNIDLLRQFAKEQGLPHYYDVGTDRYKGVCHVALAEEGFNLP
EEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCCC
GTVLFGTDSHTCTSGAFGMFGTGIGNTDAAFILGTGKLWEKVPESMKFTFEGEMPAYLTA
CEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHCCHHEEEEECCCCCCEEHH
KDLILQILGDITTDGATYRAMEFDGEAIFSLPMEERMTLTNMAIEAGGMNGIIAADNIAE
HHHHHHHHHHCCCCCCEEEEEEECCCEEEECCHHHHHHHHHHEEECCCCCCEEECHHHHH
EYVKARTKKPYEIFQSDPDAKYHSTYRYNVRDLEPVVAQPHSPDNRATVRSVAGTKITKS
HHHHHHCCCCHHHHCCCCCCCCCCEEEECCCCCCHHHCCCCCCCCCHHHHHHHCCHHHHH
YIGSCTGGKLSDFMMAAKILKGQKVTVTTTIVPATTLVARSLETEQYDGKSLKQIFEEAG
HHCCCCCCHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
CNVALPSCAACLGGPADTVGRSVDNDLVVSTTNRNFPGRMGSKHAGVYLASPLTAAASAI
CCEECCHHHHHHCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHH
TGKLTDPRDFL
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]