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The map label for this gene is menD

Identifier: 78189656

GI number: 78189656

Start: 2208303

End: 2210054

Strand: Reverse

Name: menD

Synonym: Cag_1700

Alternate gene names: 78189656

Gene position: 2210054-2208303 (Counterclockwise)

Preceding gene: 78189657

Following gene: 78189655

Centisome position: 85.92

GC content: 49.09

Gene sequence:

>1752_bases
ATGAATAATCGGCAGATAACCACCTTGTGGAGCACCCTGCTTGTTGAAGCATTGATTCGTCAGGGCGTTGATTTTTTTTG
CATTTCTCCCGGCTCTCGCTCTACTCCGCTTACCATTGCTGCCGCTCGCAATCCTCGTGCCCGCACAAAGCTTTTTGCCG
ATGAGCGCTCGGCAGCTTTTTTTGCGCTTGGTTATGCACGCGCAACGGAAATGGTTGCTCCTCTGATTTGCACCTCAGGC
ACTGCGGTCGCAAACTACTTTCCTGCGGTGGTTGAAGCCTCTATGGATTTTCAGCCTATGCTCATTATATCGGCTGATCG
CCCTTTTGAATTGCTTGAATGTGGGGCAAACCAAACCATTCGTCAAGAGCATATTTTTGGAAGCTACACTCGCTGGAGTA
TGCAACTGCCTACGCCATCGGTTGAGGTGCCACTAACCTCGCTTCTTTCTACGGTAGAATATGCTGTTGCAAAAGCCCTC
AATACTCCTGCGGGAGTGGTGCATCTCAATCAGCCATTTCGTGAGCCATTTGAGCCTGAGAGCGTTGCGGCAAACAACCA
CGCGTGGTGGCAAAGCGCTCAGCAATGGCTTGCAAGCAAAGCAGCACACACAACCACAACGGTAGAAAAAAAGCATCCCA
ACAATGCCTCCATCACTCTGCTGCGCCAGCACCTTACAACCGCAAAGCAGCCGCTCCTGATTGCGGGAAGTATGCGTTGC
AAAGATGATGCTGAAGCTGTTGCGGCACTTGCAAACAATTTGAAAATTCCACTCTATGCGGATTTTTCATCGGGGTTGCG
CATGAAGAGCAACCTGCCGCCCTTGCAATTGCTCATGCAATCGCCTGCATGGCGTGCAGCATTTCATCCTGATGTTGTGC
TTCATTTTGGAGGAAATGTGGTGGCTAAACATCTTGCAACCGCATTACGTGAATGGCAACCAGCCCACTACATGGTGGTG
CGCGAAGAGCCAATGCGCTTTTCGCCCGACCATAACGTAACCCACCGCCTTGAAGCCTCCATTGCAGCAACGGCTCATGC
GCTCCATAACAGCCGTTCTACGCCATTGTGGCGCGAAAGCGAAGCCGACCACTTTTTTGCTCAAGCGGCTCAAGAGTTGG
AAGGAGATGTAGTAGCAGATCAGCCAATCACGGAAATTTCAGCCGCACGCCTTATTTCGCGCCATATTGGTACCGAGCAA
GCGCTTTTTGTTTCAAATAGCATGGCAGTGCGCGATATGGATATGTATGCCGCAAGCTTACACGAAGCAGGCATTCCTAC
CGCTATAAATCGTGGGGCAAGTGGCATTGATGGCATTCTTTCAACTGCCGCTGGTTTTGCTTGCGGGCATGGTAAAAGCA
CCACCTTGCTGATTGGCGATATTGCTTTTTTGCACGATCTGAATGCGCTTTCGTTACTTGGTTCATTAACGGTGCCGCTA
CAAATTGTGTTACTTAATAATAATGGTGGGGGAATTTTTTCATTTCTTCCTATTGCCGCTTGTGATGATCTGTTTGAAAC
CTATTTTGCCACACCACAGCACTACAGCATTCCACTTGCGGCTGAAACTTTTGGTTTGCATTATGCCAACCCCACCACCA
ATAGTGAATTTGTAGCAGCGTACCATCAAGCACAACAAAGCCCACAAAGTACTATTATTGAGGTAAAAAGTTCACGCACC
AATAACCTTCAGCACCATCGCTTGCTTAACGCACGCTTACAAGCCATTGCAGCAAAGCTTTTTAATGGATAG

Upstream 100 bases:

>100_bases
ACTCTGGGGCTGGTTTAGTAAAAGGTTCCAATCCCGATGCGGAGTGGGAAGAAATTGAGCAAAAAATTGGCGACTTGCTC
TCCATAACCCGCATGAAGCA

Downstream 100 bases:

>100_bases
CTTCTGCCAACAACTTTTTTCATGCTTTAGCTTAACTACCCGACGCACCATGAAACCAACATTTCATTTCACCCTTATTT
GTTGTGCTGTTGCACTTTTG

Product: menaquinone biosynthesis protein

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MNNRQITTLWSTLLVEALIRQGVDFFCISPGSRSTPLTIAAARNPRARTKLFADERSAAFFALGYARATEMVAPLICTSG
TAVANYFPAVVEASMDFQPMLIISADRPFELLECGANQTIRQEHIFGSYTRWSMQLPTPSVEVPLTSLLSTVEYAVAKAL
NTPAGVVHLNQPFREPFEPESVAANNHAWWQSAQQWLASKAAHTTTTVEKKHPNNASITLLRQHLTTAKQPLLIAGSMRC
KDDAEAVAALANNLKIPLYADFSSGLRMKSNLPPLQLLMQSPAWRAAFHPDVVLHFGGNVVAKHLATALREWQPAHYMVV
REEPMRFSPDHNVTHRLEASIAATAHALHNSRSTPLWRESEADHFFAQAAQELEGDVVADQPITEISAARLISRHIGTEQ
ALFVSNSMAVRDMDMYAASLHEAGIPTAINRGASGIDGILSTAAGFACGHGKSTTLLIGDIAFLHDLNALSLLGSLTVPL
QIVLLNNNGGGIFSFLPIAACDDLFETYFATPQHYSIPLAAETFGLHYANPTTNSEFVAAYHQAQQSPQSTIIEVKSSRT
NNLQHHRLLNARLQAIAAKLFNG

Sequences:

>Translated_583_residues
MNNRQITTLWSTLLVEALIRQGVDFFCISPGSRSTPLTIAAARNPRARTKLFADERSAAFFALGYARATEMVAPLICTSG
TAVANYFPAVVEASMDFQPMLIISADRPFELLECGANQTIRQEHIFGSYTRWSMQLPTPSVEVPLTSLLSTVEYAVAKAL
NTPAGVVHLNQPFREPFEPESVAANNHAWWQSAQQWLASKAAHTTTTVEKKHPNNASITLLRQHLTTAKQPLLIAGSMRC
KDDAEAVAALANNLKIPLYADFSSGLRMKSNLPPLQLLMQSPAWRAAFHPDVVLHFGGNVVAKHLATALREWQPAHYMVV
REEPMRFSPDHNVTHRLEASIAATAHALHNSRSTPLWRESEADHFFAQAAQELEGDVVADQPITEISAARLISRHIGTEQ
ALFVSNSMAVRDMDMYAASLHEAGIPTAINRGASGIDGILSTAAGFACGHGKSTTLLIGDIAFLHDLNALSLLGSLTVPL
QIVLLNNNGGGIFSFLPIAACDDLFETYFATPQHYSIPLAAETFGLHYANPTTNSEFVAAYHQAQQSPQSTIIEVKSSRT
NNLQHHRLLNARLQAIAAKLFNG
>Mature_583_residues
MNNRQITTLWSTLLVEALIRQGVDFFCISPGSRSTPLTIAAARNPRARTKLFADERSAAFFALGYARATEMVAPLICTSG
TAVANYFPAVVEASMDFQPMLIISADRPFELLECGANQTIRQEHIFGSYTRWSMQLPTPSVEVPLTSLLSTVEYAVAKAL
NTPAGVVHLNQPFREPFEPESVAANNHAWWQSAQQWLASKAAHTTTTVEKKHPNNASITLLRQHLTTAKQPLLIAGSMRC
KDDAEAVAALANNLKIPLYADFSSGLRMKSNLPPLQLLMQSPAWRAAFHPDVVLHFGGNVVAKHLATALREWQPAHYMVV
REEPMRFSPDHNVTHRLEASIAATAHALHNSRSTPLWRESEADHFFAQAAQELEGDVVADQPITEISAARLISRHIGTEQ
ALFVSNSMAVRDMDMYAASLHEAGIPTAINRGASGIDGILSTAAGFACGHGKSTTLLIGDIAFLHDLNALSLLGSLTVPL
QIVLLNNNGGGIFSFLPIAACDDLFETYFATPQHYSIPLAAETFGLHYANPTTNSEFVAAYHQAQQSPQSTIIEVKSSRT
NNLQHHRLLNARLQAIAAKLFNG

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=562, Percent_Identity=34.8754448398576, Blast_Score=290, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_CHLCH (Q3APX4)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_379994.1
- ProteinModelPortal:   Q3APX4
- STRING:   Q3APX4
- GeneID:   3746389
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1700
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   CLSK637963
- BioCyc:   CCHL340177:CAG_1700-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 63622; Mature: 63622

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRQITTLWSTLLVEALIRQGVDFFCISPGSRSTPLTIAAARNPRARTKLFADERSAAF
CCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHCCCCCEE
FALGYARATEMVAPLICTSGTAVANYFPAVVEASMDFQPMLIISADRPFELLECGANQTI
EEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCCH
RQEHIFGSYTRWSMQLPTPSVEVPLTSLLSTVEYAVAKALNTPAGVVHLNQPFREPFEPE
HHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
SVAANNHAWWQSAQQWLASKAAHTTTTVEKKHPNNASITLLRQHLTTAKQPLLIAGSMRC
CCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCC
KDDAEAVAALANNLKIPLYADFSSGLRMKSNLPPLQLLMQSPAWRAAFHPDVVLHFGGNV
CHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCHHHHHHCCCCCCCCCCCCEEEEECCHH
VAKHLATALREWQPAHYMVVREEPMRFSPDHNVTHRLEASIAATAHALHNSRSTPLWRES
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
EADHFFAQAAQELEGDVVADQPITEISAARLISRHIGTEQALFVSNSMAVRDMDMYAASL
CCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHH
HEAGIPTAINRGASGIDGILSTAAGFACGHGKSTTLLIGDIAFLHDLNALSLLGSLTVPL
HHCCCCHHHHCCCCCHHHHHHHHHCCEECCCCCCEEEEEHHHHHHCCHHHHHHHCCCCEE
QIVLLNNNGGGIFSFLPIAACDDLFETYFATPQHYSIPLAAETFGLHYANPTTNSEFVAA
EEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHCCEEECCCCCCCHHHHH
YHQAQQSPQSTIIEVKSSRTNNLQHHRLLNARLQAIAAKLFNG
HHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNRQITTLWSTLLVEALIRQGVDFFCISPGSRSTPLTIAAARNPRARTKLFADERSAAF
CCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHCCCCCEE
FALGYARATEMVAPLICTSGTAVANYFPAVVEASMDFQPMLIISADRPFELLECGANQTI
EEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCCH
RQEHIFGSYTRWSMQLPTPSVEVPLTSLLSTVEYAVAKALNTPAGVVHLNQPFREPFEPE
HHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
SVAANNHAWWQSAQQWLASKAAHTTTTVEKKHPNNASITLLRQHLTTAKQPLLIAGSMRC
CCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCC
KDDAEAVAALANNLKIPLYADFSSGLRMKSNLPPLQLLMQSPAWRAAFHPDVVLHFGGNV
CHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCHHHHHHCCCCCCCCCCCCEEEEECCHH
VAKHLATALREWQPAHYMVVREEPMRFSPDHNVTHRLEASIAATAHALHNSRSTPLWRES
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
EADHFFAQAAQELEGDVVADQPITEISAARLISRHIGTEQALFVSNSMAVRDMDMYAASL
CCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHH
HEAGIPTAINRGASGIDGILSTAAGFACGHGKSTTLLIGDIAFLHDLNALSLLGSLTVPL
HHCCCCHHHHCCCCCHHHHHHHHHCCEECCCCCCEEEEEHHHHHHCCHHHHHHHCCCCEE
QIVLLNNNGGGIFSFLPIAACDDLFETYFATPQHYSIPLAAETFGLHYANPTTNSEFVAA
EEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHCCEEECCCCCCCHHHHH
YHQAQQSPQSTIIEVKSSRTNNLQHHRLLNARLQAIAAKLFNG
HHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA