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The map label for this gene is menF [H]

Identifier: 78189657

GI number: 78189657

Start: 2210051

End: 2211499

Strand: Reverse

Name: menF [H]

Synonym: Cag_1701

Alternate gene names: 78189657

Gene position: 2211499-2210051 (Counterclockwise)

Preceding gene: 78189658

Following gene: 78189656

Centisome position: 85.98

GC content: 45.34

Gene sequence:

>1449_bases
ATGCCAACAACTACACCAAACGATGAATCCCATATCCACCTTACAACAAGGGAGCAATTACCTCTTGCAATAGCCCTTGA
ACATTTAAAGGATTCCGTAAAAGCGATACGTCGCTCTCTGCCTTATCATAAGGCTCAAAAAGCACAGGTTATTACCTGCG
TAGAGGCTATTGAGCCAATAGAGCCGCTGCATTGGCTTTACGTTCAACAGCTTTACCCTCGCCTTTATTGGAAAAATCGT
GAGCAAAACAATGAGGTTGCGGCGGTTGGTATTGCTGATAGCTTTACCGCTAAAGATGATGATAACAATAACAGTGCTTT
TGCTGCGTTTCAAGCTGCATGTGATGAACGCTCTCCAGCCCTCCGTTATTTTGGTGGATTCCGCTTTAATGGTGCGGAAT
TATCGGAGGAGCATTGGAAGCACTTTCCCCCTTTTTTCTTTTTTCTCCCTCTTGTAACGGTTGAGCGTTTCGCTGACCAA
CGTTACACGGTAAGTGCTAATCTTTTTTTAGCTGTCGGCGATAGTGTTGATGAAAAAATCAATCAGCTTATAGCCTTTAT
TGAACAACTCAATAGTAGGATTGATAATGAGAGTGTGCTTAACACGCTGTTGCCCTCGGTTGAGCACATTACCCGCACGC
CAAATAAAGCGGCATGGAATGCAGGGTGCGAAAAAGCGCTTAATGCCTTTGAAAATGGTGAGTTAGAAAAAATTATGTTG
GCACGCCAAACAACTCTGCGCTTTACTCATACCTTTTCACCGTTGCTTTTTTTGTTGCGTTACCCCTATCCGCACAATGC
CATTTATCGCTTTTATTTGGAGCTGGCACCAAACCAAGCTTTCTTTAGCTTTACGCCTGAACGGCTTTTTCGCCGCCATG
AAAGCATGGTTGAAACGGAGGCGCTTGCAGGTACTTGCTCAAAGGAGCTGCTTCAAGCAACCGATCAGCACGCTTCGCAA
ACATTGTTGGGTTCCGAAAAAGATGTTCGTGAGCACAATTATGTGCGGGAAATGATTTACCAAGAGCTTCTGCCAATTTG
TAGCTCGATTGATATGGAGGATGAGGTGCAAGTGTTGCAACTGCAACGTTTAGCGCATCTTTATACCCGTTGCTCGGCAA
CTTTGAAAGAAAAGAGTATTCCCACCAGCGATATTCTCAAGCAATTGCATCCAACGCCTGCCGTTGGTGGCGTGCCAAAA
GAGGAGATTTTAGAACATATTCGTGCGCTGGAGCCATTTACTCGTGGTTGGTATGCGGCGCCTGTTGGTTGGGTTGATAG
CCATCATGCAGAATTTGCAGTAGCCATTCGATCAGGATTAGTGAATGGCAATGAGGTGCATCTCTACTCTGGGGCTGGTT
TAGTAAAAGGTTCCAATCCCGATGCGGAGTGGGAAGAAATTGAGCAAAAAATTGGCGACTTGCTCTCCATAACCCGCATG
AAGCAATGA

Upstream 100 bases:

>100_bases
GATAAAGAGAATCAAGCACCAAAGTAATATAAGGTGTCGCCACTGTAGTATTCACATCCGCCATCACCCAAGAGGACAAA
CGTGTTGAAGTAGCAACATC

Downstream 100 bases:

>100_bases
ATAATCGGCAGATAACCACCTTGTGGAGCACCCTGCTTGTTGAAGCATTGATTCGTCAGGGCGTTGATTTTTTTTGCATT
TCTCCCGGCTCTCGCTCTAC

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MPTTTPNDESHIHLTTREQLPLAIALEHLKDSVKAIRRSLPYHKAQKAQVITCVEAIEPIEPLHWLYVQQLYPRLYWKNR
EQNNEVAAVGIADSFTAKDDDNNNSAFAAFQAACDERSPALRYFGGFRFNGAELSEEHWKHFPPFFFFLPLVTVERFADQ
RYTVSANLFLAVGDSVDEKINQLIAFIEQLNSRIDNESVLNTLLPSVEHITRTPNKAAWNAGCEKALNAFENGELEKIML
ARQTTLRFTHTFSPLLFLLRYPYPHNAIYRFYLELAPNQAFFSFTPERLFRRHESMVETEALAGTCSKELLQATDQHASQ
TLLGSEKDVREHNYVREMIYQELLPICSSIDMEDEVQVLQLQRLAHLYTRCSATLKEKSIPTSDILKQLHPTPAVGGVPK
EEILEHIRALEPFTRGWYAAPVGWVDSHHAEFAVAIRSGLVNGNEVHLYSGAGLVKGSNPDAEWEEIEQKIGDLLSITRM
KQ

Sequences:

>Translated_482_residues
MPTTTPNDESHIHLTTREQLPLAIALEHLKDSVKAIRRSLPYHKAQKAQVITCVEAIEPIEPLHWLYVQQLYPRLYWKNR
EQNNEVAAVGIADSFTAKDDDNNNSAFAAFQAACDERSPALRYFGGFRFNGAELSEEHWKHFPPFFFFLPLVTVERFADQ
RYTVSANLFLAVGDSVDEKINQLIAFIEQLNSRIDNESVLNTLLPSVEHITRTPNKAAWNAGCEKALNAFENGELEKIML
ARQTTLRFTHTFSPLLFLLRYPYPHNAIYRFYLELAPNQAFFSFTPERLFRRHESMVETEALAGTCSKELLQATDQHASQ
TLLGSEKDVREHNYVREMIYQELLPICSSIDMEDEVQVLQLQRLAHLYTRCSATLKEKSIPTSDILKQLHPTPAVGGVPK
EEILEHIRALEPFTRGWYAAPVGWVDSHHAEFAVAIRSGLVNGNEVHLYSGAGLVKGSNPDAEWEEIEQKIGDLLSITRM
KQ
>Mature_481_residues
PTTTPNDESHIHLTTREQLPLAIALEHLKDSVKAIRRSLPYHKAQKAQVITCVEAIEPIEPLHWLYVQQLYPRLYWKNRE
QNNEVAAVGIADSFTAKDDDNNNSAFAAFQAACDERSPALRYFGGFRFNGAELSEEHWKHFPPFFFFLPLVTVERFADQR
YTVSANLFLAVGDSVDEKINQLIAFIEQLNSRIDNESVLNTLLPSVEHITRTPNKAAWNAGCEKALNAFENGELEKIMLA
RQTTLRFTHTFSPLLFLLRYPYPHNAIYRFYLELAPNQAFFSFTPERLFRRHESMVETEALAGTCSKELLQATDQHASQT
LLGSEKDVREHNYVREMIYQELLPICSSIDMEDEVQVLQLQRLAHLYTRCSATLKEKSIPTSDILKQLHPTPAVGGVPKE
EILEHIRALEPFTRGWYAAPVGWVDSHHAEFAVAIRSGLVNGNEVHLYSGAGLVKGSNPDAEWEEIEQKIGDLLSITRMK
Q

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=431, Percent_Identity=31.322505800464, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1786809, Length=247, Percent_Identity=29.5546558704453, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788114, Length=199, Percent_Identity=23.6180904522613, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=210, Percent_Identity=27.6190476190476, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 54932; Mature: 54801

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTTPNDESHIHLTTREQLPLAIALEHLKDSVKAIRRSLPYHKAQKAQVITCVEAIEPI
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
EPLHWLYVQQLYPRLYWKNREQNNEVAAVGIADSFTAKDDDNNNSAFAAFQAACDERSPA
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCH
LRYFGGFRFNGAELSEEHWKHFPPFFFFLPLVTVERFADQRYTVSANLFLAVGDSVDEKI
HHHHCCEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCHHHHHH
NQLIAFIEQLNSRIDNESVLNTLLPSVEHITRTPNKAAWNAGCEKALNAFENGELEKIML
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
ARQTTLRFTHTFSPLLFLLRYPYPHNAIYRFYLELAPNQAFFSFTPERLFRRHESMVETE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHH
ALAGTCSKELLQATDQHASQTLLGSEKDVREHNYVREMIYQELLPICSSIDMEDEVQVLQ
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LQRLAHLYTRCSATLKEKSIPTSDILKQLHPTPAVGGVPKEEILEHIRALEPFTRGWYAA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHCCCEEC
PVGWVDSHHAEFAVAIRSGLVNGNEVHLYSGAGLVKGSNPDAEWEEIEQKIGDLLSITRM
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHC
KQ
CC
>Mature Secondary Structure 
PTTTPNDESHIHLTTREQLPLAIALEHLKDSVKAIRRSLPYHKAQKAQVITCVEAIEPI
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
EPLHWLYVQQLYPRLYWKNREQNNEVAAVGIADSFTAKDDDNNNSAFAAFQAACDERSPA
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCH
LRYFGGFRFNGAELSEEHWKHFPPFFFFLPLVTVERFADQRYTVSANLFLAVGDSVDEKI
HHHHCCEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCHHHHHH
NQLIAFIEQLNSRIDNESVLNTLLPSVEHITRTPNKAAWNAGCEKALNAFENGELEKIML
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
ARQTTLRFTHTFSPLLFLLRYPYPHNAIYRFYLELAPNQAFFSFTPERLFRRHESMVETE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHH
ALAGTCSKELLQATDQHASQTLLGSEKDVREHNYVREMIYQELLPICSSIDMEDEVQVLQ
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LQRLAHLYTRCSATLKEKSIPTSDILKQLHPTPAVGGVPKEEILEHIRALEPFTRGWYAA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHCCCEEC
PVGWVDSHHAEFAVAIRSGLVNGNEVHLYSGAGLVKGSNPDAEWEEIEQKIGDLLSITRM
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHC
KQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]