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The map label for this gene is leuS

Identifier: 78189645

GI number: 78189645

Start: 2190422

End: 2192839

Strand: Reverse

Name: leuS

Synonym: Cag_1688

Alternate gene names: 78189645

Gene position: 2192839-2190422 (Counterclockwise)

Preceding gene: 78189646

Following gene: 78189644

Centisome position: 85.26

GC content: 48.64

Gene sequence:

>2418_bases
ATGAAATACGACTTCACCTCTATAGAAAAAAAATGGCAAACTCGTTGGCAAGCTGAAGCCACCTTTGCCACTGGTACAGA
CCACAGTAAACCAAAATATTATGTGCTCGACATGTTCCCTTACCCAAGTGGAAGTGGCTTACATGTTGGTCATCTTGAAG
GATATACTGCCTCCGATATTATTGCGCGTTACAAGCGAAGCAGTGGTTACAATGTGCTTCACCCAATGGGGTGGGACGCT
TTTGGTTTACCTGCTGAGCAGTTCGCTATTAAAACAGGCACTCATCCTCGCATTACCACTGAAGCGAACGTCAAGAACTT
TAAAGGCACGCTTCAGGCAATGGGCTTTTCCTACGATTGGGAACGGGAAATCAATACGACTGATTCGGGCTACTTTAAAT
GGACGCAGTGGATTTTTTTACAGCTCTACGATCGTGGTTTAGCCTACATGTCGGAAGTGGATGTGAATTGGTGTGAGGAG
CTGAAAACCGTGCTTGCCAACGAGGAGGTTGATGAAAAACTTGCCGATGGCTACACGGTGGTACGGCGTCCGTTGCGGCA
GTGGGTGCTTAAAATTACCGCGTATGCTGAGCGCTTGTTGGCTGATTTGGAGGAGCTTGATTGGCCTGAAAATGTGAAGC
AGATGCAGCGCAATTGGATTGGGCGCTCCGAAGGTGTGGAAATTGATTTTGAACTTCGTTGCCATCGCACAACGCTGAAA
GCTTACACCACGCGCCCCGACACGCTTTTTGGGGCAACCTACCTTGTTATTGCGCCCGAACATCCAATGGCAGAAAAGCT
TGCCACCGCTCCGCAACTGCTTGTGGTTAAAGAGTATATCACCAAAGCAAAGCTAAAAAGCGATTTGGAACGCACGGGGT
TGCAAAAGGAGAAAAGCGGCGTTTTCACGGGTTCGTATGCCATAAATCCCGCAACAGGTCAGCCACTTCCCATTTGGATT
TCCGACTTTGTGCTGATTAGCTACGGCACGGGCGCCATTATGTCGGTGCCAGCGCACGATAGCCGCGACTGGGCATTTGC
AAAGCAATACAATCTGCCCATTATCGAAGTCATTAAAAGTCCGCACGATGTGCAAGAGGCTGTGTTTGAAGAGAAAAACA
GCACATGCGTAAACTCCGCAAACGATGAAATTTCTCTCAACGGGCTTGATTTTGCTACGGCATTTGAGCGTATGGCAACA
TGGTTGGAGTCCAAAAAAGTGGGCAAGCGCAAGGTAAACTACAAATTGCGCGATTGGATTTTTAGCCGTCAACGCTACTG
GGGTGAGCCAATTCCCATTAAACATTATGAGGATGGAACTATTCGCCCCGAAACCAATTTGCCATTGGAATTACCAGCGG
TTGAAGCCTATCACCCCACCTCTACGGGTGAGTCACCGCTTGCCAACATCACAGAGTGGCTCATAGGTAATGACGAACAT
GGTGCCTTCCGCCGCGAAACCAACACCATGCCGCAATGGGCAGGCAGTTGCTGGTACTACCTCCGCTTTATTGATCCCCA
CAACCATGCTCAAGTAGTGGATGGCAATAACGAACGTTACTGGATGAATGTTGACCTCTACATTGGTGGCGCCGAACATG
CCGTGCTGCACCTGCTCTACTCCCGCTTTTGGCACAAAGTGCTTTACGACCTTGGCGTGGTAAGCACCAAAGAGCCATTC
CAAAAGCTCTTTAATCAAGGGATGATTTTGGGTGAGGATAATGAGAAGATGTCGAAATCACGCGGTAACGTTATTCCCGC
TGACCATGTGCTACAACGCTACGGAGCCGATGCCGTCCGCTTGTACGAAATGTTCCTTGGACCGCTTGAGCAGGTAAAGC
CTTGGAACACCAACGGTATTGAAGGCATTAGCCGCTTTCTTGGCAAAGTGTGGCGCTTTGTTTATCCCGAACATAGCGAA
GCCGCAACCCAGCCCAGCAATGAGCCACTGCCCGATGAGTTGCTTCGCCGAATGCACAAAACCATTAAAAAGGTTGGTGA
TGACACAAGCAGCCTAAAGTTTAACACGGCTATTGCGGAAATGATGGTCTTTGTAAACGAGCTAACCAAAACGGGCTGCA
ACAACCGCGAAGCCATTGAAACCCTGCTCAAGCTTTTAGCGCCTTACGCTCCCCACATGACCGAAGAGCTATGGGAAGCA
CTTGGGCACACCAATTCCATCAGCCATGAACCATTCCCAACCTTTAATCCTGCATTGGTTGAAGAAAACATGGCAATCAT
TGCCGTACAGGTAAATGGCAAACTTCGTGGCACCTTTACCGTGCCAGCAAAAAGCCCCAAAGAGATGTTGCTTGAAGAAG
CACGCAAGGTTGAATCGGTTGCAAAATTCCTTGAAGGCAAAACCATTGTTAAGGAAATTGTTGTGCCCGATAAGCTGGTA
AATTTTGCGGTGAAGTAA

Upstream 100 bases:

>100_bases
GGGTGGCGAAGCAACGCAAAACTAACGAAAGCTCTTTTTACAAAGCAGCACTGCTCAAACGGTGCTGCTTTTTTTATTTC
AGAAAAAATAGAAATGACTT

Downstream 100 bases:

>100_bases
AGGGGGGAAGCTAAAACAAGTAACAACATGAATACGCCAATTGAATTTATGAAGCCTCGTTTGGTAGGCGATCGTTTTTC
AGGACATGCTATTCCCTTTG

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 805; Mature: 805

Protein sequence:

>805_residues
MKYDFTSIEKKWQTRWQAEATFATGTDHSKPKYYVLDMFPYPSGSGLHVGHLEGYTASDIIARYKRSSGYNVLHPMGWDA
FGLPAEQFAIKTGTHPRITTEANVKNFKGTLQAMGFSYDWEREINTTDSGYFKWTQWIFLQLYDRGLAYMSEVDVNWCEE
LKTVLANEEVDEKLADGYTVVRRPLRQWVLKITAYAERLLADLEELDWPENVKQMQRNWIGRSEGVEIDFELRCHRTTLK
AYTTRPDTLFGATYLVIAPEHPMAEKLATAPQLLVVKEYITKAKLKSDLERTGLQKEKSGVFTGSYAINPATGQPLPIWI
SDFVLISYGTGAIMSVPAHDSRDWAFAKQYNLPIIEVIKSPHDVQEAVFEEKNSTCVNSANDEISLNGLDFATAFERMAT
WLESKKVGKRKVNYKLRDWIFSRQRYWGEPIPIKHYEDGTIRPETNLPLELPAVEAYHPTSTGESPLANITEWLIGNDEH
GAFRRETNTMPQWAGSCWYYLRFIDPHNHAQVVDGNNERYWMNVDLYIGGAEHAVLHLLYSRFWHKVLYDLGVVSTKEPF
QKLFNQGMILGEDNEKMSKSRGNVIPADHVLQRYGADAVRLYEMFLGPLEQVKPWNTNGIEGISRFLGKVWRFVYPEHSE
AATQPSNEPLPDELLRRMHKTIKKVGDDTSSLKFNTAIAEMMVFVNELTKTGCNNREAIETLLKLLAPYAPHMTEELWEA
LGHTNSISHEPFPTFNPALVEENMAIIAVQVNGKLRGTFTVPAKSPKEMLLEEARKVESVAKFLEGKTIVKEIVVPDKLV
NFAVK

Sequences:

>Translated_805_residues
MKYDFTSIEKKWQTRWQAEATFATGTDHSKPKYYVLDMFPYPSGSGLHVGHLEGYTASDIIARYKRSSGYNVLHPMGWDA
FGLPAEQFAIKTGTHPRITTEANVKNFKGTLQAMGFSYDWEREINTTDSGYFKWTQWIFLQLYDRGLAYMSEVDVNWCEE
LKTVLANEEVDEKLADGYTVVRRPLRQWVLKITAYAERLLADLEELDWPENVKQMQRNWIGRSEGVEIDFELRCHRTTLK
AYTTRPDTLFGATYLVIAPEHPMAEKLATAPQLLVVKEYITKAKLKSDLERTGLQKEKSGVFTGSYAINPATGQPLPIWI
SDFVLISYGTGAIMSVPAHDSRDWAFAKQYNLPIIEVIKSPHDVQEAVFEEKNSTCVNSANDEISLNGLDFATAFERMAT
WLESKKVGKRKVNYKLRDWIFSRQRYWGEPIPIKHYEDGTIRPETNLPLELPAVEAYHPTSTGESPLANITEWLIGNDEH
GAFRRETNTMPQWAGSCWYYLRFIDPHNHAQVVDGNNERYWMNVDLYIGGAEHAVLHLLYSRFWHKVLYDLGVVSTKEPF
QKLFNQGMILGEDNEKMSKSRGNVIPADHVLQRYGADAVRLYEMFLGPLEQVKPWNTNGIEGISRFLGKVWRFVYPEHSE
AATQPSNEPLPDELLRRMHKTIKKVGDDTSSLKFNTAIAEMMVFVNELTKTGCNNREAIETLLKLLAPYAPHMTEELWEA
LGHTNSISHEPFPTFNPALVEENMAIIAVQVNGKLRGTFTVPAKSPKEMLLEEARKVESVAKFLEGKTIVKEIVVPDKLV
NFAVK
>Mature_805_residues
MKYDFTSIEKKWQTRWQAEATFATGTDHSKPKYYVLDMFPYPSGSGLHVGHLEGYTASDIIARYKRSSGYNVLHPMGWDA
FGLPAEQFAIKTGTHPRITTEANVKNFKGTLQAMGFSYDWEREINTTDSGYFKWTQWIFLQLYDRGLAYMSEVDVNWCEE
LKTVLANEEVDEKLADGYTVVRRPLRQWVLKITAYAERLLADLEELDWPENVKQMQRNWIGRSEGVEIDFELRCHRTTLK
AYTTRPDTLFGATYLVIAPEHPMAEKLATAPQLLVVKEYITKAKLKSDLERTGLQKEKSGVFTGSYAINPATGQPLPIWI
SDFVLISYGTGAIMSVPAHDSRDWAFAKQYNLPIIEVIKSPHDVQEAVFEEKNSTCVNSANDEISLNGLDFATAFERMAT
WLESKKVGKRKVNYKLRDWIFSRQRYWGEPIPIKHYEDGTIRPETNLPLELPAVEAYHPTSTGESPLANITEWLIGNDEH
GAFRRETNTMPQWAGSCWYYLRFIDPHNHAQVVDGNNERYWMNVDLYIGGAEHAVLHLLYSRFWHKVLYDLGVVSTKEPF
QKLFNQGMILGEDNEKMSKSRGNVIPADHVLQRYGADAVRLYEMFLGPLEQVKPWNTNGIEGISRFLGKVWRFVYPEHSE
AATQPSNEPLPDELLRRMHKTIKKVGDDTSSLKFNTAIAEMMVFVNELTKTGCNNREAIETLLKLLAPYAPHMTEELWEA
LGHTNSISHEPFPTFNPALVEENMAIIAVQVNGKLRGTFTVPAKSPKEMLLEEARKVESVAKFLEGKTIVKEIVVPDKLV
NFAVK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=876, Percent_Identity=32.9908675799087, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI94721239, Length=939, Percent_Identity=21.0862619808307, Blast_Score=103, Evalue=8e-22,
Organism=Homo sapiens, GI94721241, Length=939, Percent_Identity=21.0862619808307, Blast_Score=103, Evalue=8e-22,
Organism=Escherichia coli, GI1786861, Length=868, Percent_Identity=41.2442396313364, Blast_Score=628, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=393, Percent_Identity=25.1908396946565, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71997517, Length=762, Percent_Identity=27.2965879265092, Blast_Score=259, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI71997510, Length=765, Percent_Identity=27.1895424836601, Blast_Score=259, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI212645227, Length=310, Percent_Identity=26.4516129032258, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17510661, Length=841, Percent_Identity=21.640903686088, Blast_Score=102, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323414, Length=811, Percent_Identity=34.7718865598027, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6321531, Length=499, Percent_Identity=22.2444889779559, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325217, Length=343, Percent_Identity=24.198250728863, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21355409, Length=842, Percent_Identity=30.6413301662708, Blast_Score=340, Evalue=3e-93,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_CHLCH (Q3APY5)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_379983.1
- HSSP:   P07813
- ProteinModelPortal:   Q3APY5
- SMR:   Q3APY5
- STRING:   Q3APY5
- GeneID:   3746378
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1688
- eggNOG:   COG0495
- HOGENOM:   HBG693845
- OMA:   ERGNFPV
- ProtClustDB:   PRK00390
- BioCyc:   CCHL340177:CAG_1688-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 92008; Mature: 92008

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 579-579

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDFTSIEKKWQTRWQAEATFATGTDHSKPKYYVLDMFPYPSGSGLHVGHLEGYTASDI
CCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHH
IARYKRSSGYNVLHPMGWDAFGLPAEQFAIKTGTHPRITTEANVKNFKGTLQAMGFSYDW
HHHHHHCCCCCEECCCCCCCCCCCHHHHEEECCCCCCEEECCCCHHHHHHHHHCCCCCCC
EREINTTDSGYFKWTQWIFLQLYDRGLAYMSEVDVNWCEELKTVLANEEVDEKLADGYTV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCHHH
VRRPLRQWVLKITAYAERLLADLEELDWPENVKQMQRNWIGRSEGVEIDFELRCHRTTLK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEEEEHEEEH
AYTTRPDTLFGATYLVIAPEHPMAEKLATAPQLLVVKEYITKAKLKSDLERTGLQKEKSG
HCCCCCCCEECCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
VFTGSYAINPATGQPLPIWISDFVLISYGTGAIMSVPAHDSRDWAFAKQYNLPIIEVIKS
EEECCEEECCCCCCCCEEEEECEEEEEECCCCEEECCCCCCCCCCCHHHCCCCHHHHHCC
PHDVQEAVFEEKNSTCVNSANDEISLNGLDFATAFERMATWLESKKVGKRKVNYKLRDWI
CHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHH
FSRQRYWGEPIPIKHYEDGTIRPETNLPLELPAVEAYHPTSTGESPLANITEWLIGNDEH
HHHHHHCCCCCCCEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
GAFRRETNTMPQWAGSCWYYLRFIDPHNHAQVVDGNNERYWMNVDLYIGGAEHAVLHLLY
CCHHCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCEEEEEEEEEECCHHHHHHHHHH
SRFWHKVLYDLGVVSTKEPFQKLFNQGMILGEDNEKMSKSRGNVIPADHVLQRYGADAVR
HHHHHHHHHHHCCCCCHHHHHHHHHCCEEECCCCHHHHHHCCCCCCHHHHHHHHCCHHHH
LYEMFLGPLEQVKPWNTNGIEGISRFLGKVWRFVYPEHSEAATQPSNEPLPDELLRRMHK
HHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
TIKKVGDDTSSLKFNTAIAEMMVFVNELTKTGCNNREAIETLLKLLAPYAPHMTEELWEA
HHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
LGHTNSISHEPFPTFNPALVEENMAIIAVQVNGKLRGTFTVPAKSPKEMLLEEARKVESV
HCCCCCCCCCCCCCCCHHHCCCCEEEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHH
AKFLEGKTIVKEIVVPDKLVNFAVK
HHHHCCHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MKYDFTSIEKKWQTRWQAEATFATGTDHSKPKYYVLDMFPYPSGSGLHVGHLEGYTASDI
CCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHH
IARYKRSSGYNVLHPMGWDAFGLPAEQFAIKTGTHPRITTEANVKNFKGTLQAMGFSYDW
HHHHHHCCCCCEECCCCCCCCCCCHHHHEEECCCCCCEEECCCCHHHHHHHHHCCCCCCC
EREINTTDSGYFKWTQWIFLQLYDRGLAYMSEVDVNWCEELKTVLANEEVDEKLADGYTV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCHHH
VRRPLRQWVLKITAYAERLLADLEELDWPENVKQMQRNWIGRSEGVEIDFELRCHRTTLK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEEEEHEEEH
AYTTRPDTLFGATYLVIAPEHPMAEKLATAPQLLVVKEYITKAKLKSDLERTGLQKEKSG
HCCCCCCCEECCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
VFTGSYAINPATGQPLPIWISDFVLISYGTGAIMSVPAHDSRDWAFAKQYNLPIIEVIKS
EEECCEEECCCCCCCCEEEEECEEEEEECCCCEEECCCCCCCCCCCHHHCCCCHHHHHCC
PHDVQEAVFEEKNSTCVNSANDEISLNGLDFATAFERMATWLESKKVGKRKVNYKLRDWI
CHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHH
FSRQRYWGEPIPIKHYEDGTIRPETNLPLELPAVEAYHPTSTGESPLANITEWLIGNDEH
HHHHHHCCCCCCCEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
GAFRRETNTMPQWAGSCWYYLRFIDPHNHAQVVDGNNERYWMNVDLYIGGAEHAVLHLLY
CCHHCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCEEEEEEEEEECCHHHHHHHHHH
SRFWHKVLYDLGVVSTKEPFQKLFNQGMILGEDNEKMSKSRGNVIPADHVLQRYGADAVR
HHHHHHHHHHHCCCCCHHHHHHHHHCCEEECCCCHHHHHHCCCCCCHHHHHHHHCCHHHH
LYEMFLGPLEQVKPWNTNGIEGISRFLGKVWRFVYPEHSEAATQPSNEPLPDELLRRMHK
HHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
TIKKVGDDTSSLKFNTAIAEMMVFVNELTKTGCNNREAIETLLKLLAPYAPHMTEELWEA
HHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
LGHTNSISHEPFPTFNPALVEENMAIIAVQVNGKLRGTFTVPAKSPKEMLLEEARKVESV
HCCCCCCCCCCCCCCCHHHCCCCEEEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHH
AKFLEGKTIVKEIVVPDKLVNFAVK
HHHHCCHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA