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The map label for this gene is pnp

Identifier: 78189646

GI number: 78189646

Start: 2192915

End: 2195101

Strand: Reverse

Name: pnp

Synonym: Cag_1689

Alternate gene names: 78189646

Gene position: 2195101-2192915 (Counterclockwise)

Preceding gene: 78189647

Following gene: 78189645

Centisome position: 85.34

GC content: 46.09

Gene sequence:

>2187_bases
ATGTTTGTTCAAAAATCAATTGATCTTGGTCACGGCAGGATTCTTTCTATCGAAACGGGCAAAATGGCAAAGCAAGCCGA
TGGTGCTGCTATTGTGCGTCTTGGCGATACTATGGTGCTTGCCACAGTGGTTTCAAGCAAAAAAACTCCTCCATTAGGTC
AAGATTTTTTTCCGTTACAGGTTGAGTATCGTGAAAAATATTCCGCTGCTGGTCGCTTTCCCGGAGGCTTTTTTAAGCGC
GAAGGTCGTCCTTCTGAAAAAGAAATTCTTTCTGCCCGCTTAATTGACCGTGCCCTTCGTCCTCTCTTTCCTGACGGCTA
TTACTACGAAACCCAAATTATTATTTCAGTTATATCATCCGATCAAGTAAATGATGGTGATGTGCTGGGTGGTCTAGCTG
CCTCTGCTGCCATTATGGTTTCCGATATTCCTTTCCAAAACCCTATGTCGGAAGTGCGTGTTGGGCGTGTTAATGGCTTG
TACATTATCAACCCCGATGTTAACGAACTAAAAGAGAGCGATATTGATATGTGCATTGGTGGTACCAACGATACCATTTG
TATGCTTGAGGGTGAAATGAATGAAATTTCGGAAACTGAAATGCTTGAGGCTATTCAGTTTGGGCACACTGCCATTCGCA
AGTTATGTGTGTTGCAACAAGAGGTTGCCGCTGAAGTTGCTAAGCCAAAGCGCCTCTTTGTTCCAACCGTTATTCCAACT
GAGCTTGATAGCTTTGTGCGTGCCAATTGCCAAAGCCGTTTACGCGAGTTGGCTTACACGCCGCTTGGCAAAGAGGAGCG
TGCTGAGCGCACGGCTGCAATTTATAACGAAACCTTAGCGGCTACGTTAGAGCATTTTCGTGCTACCATTAGCAGCGAAG
AGGCACAAGCTGATACTGCAAAAGCGCTCTGCTTAAATGAGCATATTATTGAAGATCAAATTCATGCAGTTGAAAAAGAG
GTGATGCGCCACATGATTCTTGATGATGCAAAGCGTCTTGATGGACGTGCGCTTGATCAAGTTCGCCCCATTACCATTGA
GCTTGGGCTTATTCCTCGTGCTCACGGCTCGGCACTCTTTACGCGTGGTGAAACGCAAGCGCTGGTAACTCTAACGCTTG
GCACCCAAAAAGATGCGCAATCGGTTGATAACCTCACTAATAGTGATGACAAGCGCTTTTACCTTCACTACAATTTTCCC
CCATTTAGCGTTGGCGAATGTGGACGTCTTGGCAGCATTGGTCGCCGCGAAATTGGGCATGGCAATCTTGCAGAGCGTTC
TATTAAAATGGTTGCCCCTACTGAAGCCGAATTCCCTTACACTATCCGCATTGTTTCCGACATTCTTGAATCAAACGGTT
CATCTTCAATGGCATCGGTGTGTGGAGGAACTTTAGCGTTGATGGATGGCGGCGTTCCCATTAGAAAGCCGGTGTCGGGT
ATTGCAATGGGATTGATTAAAGAGAATGATAAGTATGCGGTGCTCTCCGACATTTTAGGCAACGAGGATCACCTTGGCGA
CATGGATTTTAAAGTTTCCGGTACGCGTGATGGCATTACTGCCTGCCAAATGGATATTAAAATTGATGGGCTTGATTACC
ACATTGTGGAAAATGCTCTTGAGCAAGCTCGCAAAGGGCGCCTCCATATTCTTAATGAAATGGAAAAAGCCATTCCAGCA
TCACGTACCGATTTAGCAACTTATGCACCACGTTTAACCACCATTAAAATTCCTTCCGATTGCATTGGCATGGTGATTGG
TAAAGGTGGCGAAACCATTCGTGGCATTACCGAAGAGACGGGTGCTGAAATTAATATTGCCGATGATGGCACCGTAACCA
TTGCGTGTACGACCAAAGAGGGCACCGATGCAGCCTTAGCTACCATCAAAAGCCTGACTGCAAAACCTGAGGTTGGCAAC
ATTTACGTTGGCAAAGTGCGCGACGTGCGCGATGAACTTGGTGCCTTTGTAGAGTTCCTGCCAAAAACCGATGGCTTGGT
ACACATTTCGGAAATTTCGTCAACTCGTGTGGCTAAAGTAAGCGATCACTTAAAGGTAGGCGATAAAGTTACCGTCAAAT
TAGTTGATGTTCGCAAAGACCCACGCACCGGTAAAACGAAGTTCGCCCTTTCAATTAAAGCGCTTGAGCAGCCAAAACAA
GAGGGTGGCGAAGCAACGCAAAACTAA

Upstream 100 bases:

>100_bases
AATGACAATAAAATCAATATGAAAACTATTGGCGCTTGCTGAAAACAGGGCGTTTTTTGGCTGACTGGCGCGCTTTTCTC
ACTTCTTATCTCACTGTTTT

Downstream 100 bases:

>100_bases
CGAAAGCTCTTTTTACAAAGCAGCACTGCTCAAACGGTGCTGCTTTTTTTATTTCAGAAAAAATAGAAATGACTTATGAA
ATACGACTTCACCTCTATAG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 728; Mature: 728

Protein sequence:

>728_residues
MFVQKSIDLGHGRILSIETGKMAKQADGAAIVRLGDTMVLATVVSSKKTPPLGQDFFPLQVEYREKYSAAGRFPGGFFKR
EGRPSEKEILSARLIDRALRPLFPDGYYYETQIIISVISSDQVNDGDVLGGLAASAAIMVSDIPFQNPMSEVRVGRVNGL
YIINPDVNELKESDIDMCIGGTNDTICMLEGEMNEISETEMLEAIQFGHTAIRKLCVLQQEVAAEVAKPKRLFVPTVIPT
ELDSFVRANCQSRLRELAYTPLGKEERAERTAAIYNETLAATLEHFRATISSEEAQADTAKALCLNEHIIEDQIHAVEKE
VMRHMILDDAKRLDGRALDQVRPITIELGLIPRAHGSALFTRGETQALVTLTLGTQKDAQSVDNLTNSDDKRFYLHYNFP
PFSVGECGRLGSIGRREIGHGNLAERSIKMVAPTEAEFPYTIRIVSDILESNGSSSMASVCGGTLALMDGGVPIRKPVSG
IAMGLIKENDKYAVLSDILGNEDHLGDMDFKVSGTRDGITACQMDIKIDGLDYHIVENALEQARKGRLHILNEMEKAIPA
SRTDLATYAPRLTTIKIPSDCIGMVIGKGGETIRGITEETGAEINIADDGTVTIACTTKEGTDAALATIKSLTAKPEVGN
IYVGKVRDVRDELGAFVEFLPKTDGLVHISEISSTRVAKVSDHLKVGDKVTVKLVDVRKDPRTGKTKFALSIKALEQPKQ
EGGEATQN

Sequences:

>Translated_728_residues
MFVQKSIDLGHGRILSIETGKMAKQADGAAIVRLGDTMVLATVVSSKKTPPLGQDFFPLQVEYREKYSAAGRFPGGFFKR
EGRPSEKEILSARLIDRALRPLFPDGYYYETQIIISVISSDQVNDGDVLGGLAASAAIMVSDIPFQNPMSEVRVGRVNGL
YIINPDVNELKESDIDMCIGGTNDTICMLEGEMNEISETEMLEAIQFGHTAIRKLCVLQQEVAAEVAKPKRLFVPTVIPT
ELDSFVRANCQSRLRELAYTPLGKEERAERTAAIYNETLAATLEHFRATISSEEAQADTAKALCLNEHIIEDQIHAVEKE
VMRHMILDDAKRLDGRALDQVRPITIELGLIPRAHGSALFTRGETQALVTLTLGTQKDAQSVDNLTNSDDKRFYLHYNFP
PFSVGECGRLGSIGRREIGHGNLAERSIKMVAPTEAEFPYTIRIVSDILESNGSSSMASVCGGTLALMDGGVPIRKPVSG
IAMGLIKENDKYAVLSDILGNEDHLGDMDFKVSGTRDGITACQMDIKIDGLDYHIVENALEQARKGRLHILNEMEKAIPA
SRTDLATYAPRLTTIKIPSDCIGMVIGKGGETIRGITEETGAEINIADDGTVTIACTTKEGTDAALATIKSLTAKPEVGN
IYVGKVRDVRDELGAFVEFLPKTDGLVHISEISSTRVAKVSDHLKVGDKVTVKLVDVRKDPRTGKTKFALSIKALEQPKQ
EGGEATQN
>Mature_728_residues
MFVQKSIDLGHGRILSIETGKMAKQADGAAIVRLGDTMVLATVVSSKKTPPLGQDFFPLQVEYREKYSAAGRFPGGFFKR
EGRPSEKEILSARLIDRALRPLFPDGYYYETQIIISVISSDQVNDGDVLGGLAASAAIMVSDIPFQNPMSEVRVGRVNGL
YIINPDVNELKESDIDMCIGGTNDTICMLEGEMNEISETEMLEAIQFGHTAIRKLCVLQQEVAAEVAKPKRLFVPTVIPT
ELDSFVRANCQSRLRELAYTPLGKEERAERTAAIYNETLAATLEHFRATISSEEAQADTAKALCLNEHIIEDQIHAVEKE
VMRHMILDDAKRLDGRALDQVRPITIELGLIPRAHGSALFTRGETQALVTLTLGTQKDAQSVDNLTNSDDKRFYLHYNFP
PFSVGECGRLGSIGRREIGHGNLAERSIKMVAPTEAEFPYTIRIVSDILESNGSSSMASVCGGTLALMDGGVPIRKPVSG
IAMGLIKENDKYAVLSDILGNEDHLGDMDFKVSGTRDGITACQMDIKIDGLDYHIVENALEQARKGRLHILNEMEKAIPA
SRTDLATYAPRLTTIKIPSDCIGMVIGKGGETIRGITEETGAEINIADDGTVTIACTTKEGTDAALATIKSLTAKPEVGN
IYVGKVRDVRDELGAFVEFLPKTDGLVHISEISSTRVAKVSDHLKVGDKVTVKLVDVRKDPRTGKTKFALSIKALEQPKQ
EGGEATQN

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=736, Percent_Identity=36.820652173913, Blast_Score=443, Evalue=1e-124,
Organism=Escherichia coli, GI145693187, Length=706, Percent_Identity=49.8583569405099, Blast_Score=659, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=723, Percent_Identity=33.7482710926694, Blast_Score=341, Evalue=1e-93,
Organism=Drosophila melanogaster, GI281362905, Length=734, Percent_Identity=37.1934604904632, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651641, Length=734, Percent_Identity=37.1934604904632, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651643, Length=734, Percent_Identity=37.1934604904632, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI161079377, Length=677, Percent_Identity=36.7799113737075, Blast_Score=406, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_CHLCH (Q3APY4)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_379984.1
- HSSP:   O29756
- ProteinModelPortal:   Q3APY4
- STRING:   Q3APY4
- GeneID:   3746379
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1689
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   CCHL340177:CAG_1689-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 79363; Mature: 79363

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVQKSIDLGHGRILSIETGKMAKQADGAAIVRLGDTMVLATVVSSKKTPPLGQDFFPLQ
CCCCCCCCCCCCEEEEEECCCCHHHCCCEEEEEECCCEEEHHHHHCCCCCCCCCCCCEEE
VEYREKYSAAGRFPGGFFKREGRPSEKEILSARLIDRALRPLFPDGYYYETQIIISVISS
EHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEHEEEEEEECC
DQVNDGDVLGGLAASAAIMVSDIPFQNPMSEVRVGRVNGLYIINPDVNELKESDIDMCIG
CCCCCCCHHHHHHHHHEEEEECCCCCCCHHHHCEEEECEEEEECCCHHHHHHCCCCEEEC
GTNDTICMLEGEMNEISETEMLEAIQFGHTAIRKLCVLQQEVAAEVAKPKRLFVPTVIPT
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHH
ELDSFVRANCQSRLRELAYTPLGKEERAERTAAIYNETLAATLEHFRATISSEEAQADTA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KALCLNEHIIEDQIHAVEKEVMRHMILDDAKRLDGRALDQVRPITIELGLIPRAHGSALF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCCCEEE
TRGETQALVTLTLGTQKDAQSVDNLTNSDDKRFYLHYNFPPFSVGECGRLGSIGRREIGH
ECCCCEEEEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
GNLAERSIKMVAPTEAEFPYTIRIVSDILESNGSSSMASVCGGTLALMDGGVPIRKPVSG
CCHHHCCEEEECCCCCCCCEEHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCCCCCHHH
IAMGLIKENDKYAVLSDILGNEDHLGDMDFKVSGTRDGITACQMDIKIDGLDYHIVENAL
HHHHEEECCCCEEEHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEEEECCCCHHHHHHHH
EQARKGRLHILNEMEKAIPASRTDLATYAPRLTTIKIPSDCIGMVIGKGGETIRGITEET
HHHHCCCHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCHHHHHEEECCCCCEECCCHHHC
GAEINIADDGTVTIACTTKEGTDAALATIKSLTAKPEVGNIYVGKVRDVRDELGAFVEFL
CCEEEEECCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHH
PKTDGLVHISEISSTRVAKVSDHLKVGDKVTVKLVDVRKDPRTGKTKFALSIKALEQPKQ
CCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEEEHHHHCHHH
EGGEATQN
HCCCCCCC
>Mature Secondary Structure
MFVQKSIDLGHGRILSIETGKMAKQADGAAIVRLGDTMVLATVVSSKKTPPLGQDFFPLQ
CCCCCCCCCCCCEEEEEECCCCHHHCCCEEEEEECCCEEEHHHHHCCCCCCCCCCCCEEE
VEYREKYSAAGRFPGGFFKREGRPSEKEILSARLIDRALRPLFPDGYYYETQIIISVISS
EHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEHEEEEEEECC
DQVNDGDVLGGLAASAAIMVSDIPFQNPMSEVRVGRVNGLYIINPDVNELKESDIDMCIG
CCCCCCCHHHHHHHHHEEEEECCCCCCCHHHHCEEEECEEEEECCCHHHHHHCCCCEEEC
GTNDTICMLEGEMNEISETEMLEAIQFGHTAIRKLCVLQQEVAAEVAKPKRLFVPTVIPT
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHH
ELDSFVRANCQSRLRELAYTPLGKEERAERTAAIYNETLAATLEHFRATISSEEAQADTA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KALCLNEHIIEDQIHAVEKEVMRHMILDDAKRLDGRALDQVRPITIELGLIPRAHGSALF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCCCEEE
TRGETQALVTLTLGTQKDAQSVDNLTNSDDKRFYLHYNFPPFSVGECGRLGSIGRREIGH
ECCCCEEEEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
GNLAERSIKMVAPTEAEFPYTIRIVSDILESNGSSSMASVCGGTLALMDGGVPIRKPVSG
CCHHHCCEEEECCCCCCCCEEHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCCCCCHHH
IAMGLIKENDKYAVLSDILGNEDHLGDMDFKVSGTRDGITACQMDIKIDGLDYHIVENAL
HHHHEEECCCCEEEHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEEEECCCCHHHHHHHH
EQARKGRLHILNEMEKAIPASRTDLATYAPRLTTIKIPSDCIGMVIGKGGETIRGITEET
HHHHCCCHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCHHHHHEEECCCCCEECCCHHHC
GAEINIADDGTVTIACTTKEGTDAALATIKSLTAKPEVGNIYVGKVRDVRDELGAFVEFL
CCEEEEECCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHH
PKTDGLVHISEISSTRVAKVSDHLKVGDKVTVKLVDVRKDPRTGKTKFALSIKALEQPKQ
CCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEEEHHHHCHHH
EGGEATQN
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA