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The map label for this gene is grpE [H]

Identifier: 78189386

GI number: 78189386

Start: 1892703

End: 1893350

Strand: Reverse

Name: grpE [H]

Synonym: Cag_1423

Alternate gene names: 78189386

Gene position: 1893350-1892703 (Counterclockwise)

Preceding gene: 78189387

Following gene: 78189385

Centisome position: 73.61

GC content: 46.6

Gene sequence:

>648_bases
ATGAAAAAACGCTTATTACGAAATCTTTTCACCAAACAACATACTATGGACAATATACATACCGTACCGGGCTATAACAG
CGATGCCAATCAACCGCCGCAAGAGCCAACCGCCGAAAATATTCCATTGGCTGAAGGGGTAAGTGGCAATGCAAACATGC
CGCTTGAAAGCCGCATTGCCGAACTTGAAGCAGAGCTTGAGCAGCAAAAAGAGCAAGTTGCAAAGTTCCGTGAAGAGGTG
CTCCGCAAAGCGGCTGACTTTGAAAACTTCCGCCGCCAAAAAGAGCGCGAAATTACCCTGACGGCATCACGAGCCTTTGA
AAACGTCATTCGCGATTTACTACCCTTGGTGGACGATATTCGTCGCCTTTTGCACCATGCCCCGCCCGAAGGCGAAGCGG
CACAAATTGCTCGCCCTTACATTGAAGGGGTTGAAATGGTGCAAAAAAATCTTGAAAAGTGGTTGAACGAAAAAGGCGTT
GTACCTATTGAATCGAAAGGCATGAAGCTTGATGTCAACTTCCATGAAGCCATTTCACAAATGGAGCATCCCGATGCTGA
AGCTGATACCGTTATTGAAGAGTACCAAACTGGCTACCTACTTGGCGATAAAGTAATTCGCCACGCCAAAGTAATTGTTG
CACGTTAA

Upstream 100 bases:

>100_bases
GCGCATTTTGCACTACATGGCAGGAAGTTTATCCTCCACCTTATCAATTCAGAACTAAGATTTTTTTACTCACTAACATT
GTGGGCAATCGTTTTCATAC

Downstream 100 bases:

>100_bases
CGCATATTTTTAGATTGACTTAAGATGTTCCTATGAAGAAAGATTATTATGAAACATTGGGCGTAACCCGCTCATCAAAT
AAAGACGATATTAAAAAGGC

Product: molecular chaperone GrpE (heat shock protein)-like protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKKRLLRNLFTKQHTMDNIHTVPGYNSDANQPPQEPTAENIPLAEGVSGNANMPLESRIAELEAELEQQKEQVAKFREEV
LRKAADFENFRRQKEREITLTASRAFENVIRDLLPLVDDIRRLLHHAPPEGEAAQIARPYIEGVEMVQKNLEKWLNEKGV
VPIESKGMKLDVNFHEAISQMEHPDAEADTVIEEYQTGYLLGDKVIRHAKVIVAR

Sequences:

>Translated_215_residues
MKKRLLRNLFTKQHTMDNIHTVPGYNSDANQPPQEPTAENIPLAEGVSGNANMPLESRIAELEAELEQQKEQVAKFREEV
LRKAADFENFRRQKEREITLTASRAFENVIRDLLPLVDDIRRLLHHAPPEGEAAQIARPYIEGVEMVQKNLEKWLNEKGV
VPIESKGMKLDVNFHEAISQMEHPDAEADTVIEEYQTGYLLGDKVIRHAKVIVAR
>Mature_215_residues
MKKRLLRNLFTKQHTMDNIHTVPGYNSDANQPPQEPTAENIPLAEGVSGNANMPLESRIAELEAELEQQKEQVAKFREEV
LRKAADFENFRRQKEREITLTASRAFENVIRDLLPLVDDIRRLLHHAPPEGEAAQIARPYIEGVEMVQKNLEKWLNEKGV
VPIESKGMKLDVNFHEAISQMEHPDAEADTVIEEYQTGYLLGDKVIRHAKVIVAR

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=166, Percent_Identity=33.1325301204819, Blast_Score=79, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=162, Percent_Identity=30.2469135802469, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653432, Length=159, Percent_Identity=29.559748427673, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 24580; Mature: 24580

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRLLRNLFTKQHTMDNIHTVPGYNSDANQPPQEPTAENIPLAEGVSGNANMPLESRIA
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
ELEAELEQQKEQVAKFREEVLRKAADFENFRRQKEREITLTASRAFENVIRDLLPLVDDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRLLHHAPPEGEAAQIARPYIEGVEMVQKNLEKWLNEKGVVPIESKGMKLDVNFHEAISQ
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEECCHHHHHHH
MEHPDAEADTVIEEYQTGYLLGDKVIRHAKVIVAR
HCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKRLLRNLFTKQHTMDNIHTVPGYNSDANQPPQEPTAENIPLAEGVSGNANMPLESRIA
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
ELEAELEQQKEQVAKFREEVLRKAADFENFRRQKEREITLTASRAFENVIRDLLPLVDDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRLLHHAPPEGEAAQIARPYIEGVEMVQKNLEKWLNEKGVVPIESKGMKLDVNFHEAISQ
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEECCHHHHHHH
MEHPDAEADTVIEEYQTGYLLGDKVIRHAKVIVAR
HCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA