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The map label for this gene is bglIIM [H]

Identifier: 78189365

GI number: 78189365

Start: 1866280

End: 1867074

Strand: Reverse

Name: bglIIM [H]

Synonym: Cag_1402

Alternate gene names: 78189365

Gene position: 1867074-1866280 (Counterclockwise)

Preceding gene: 78189366

Following gene: 78189364

Centisome position: 72.59

GC content: 32.7

Gene sequence:

>795_bases
ATGAAATTTCCTGATGATTATATCAATACCATTATTTGTGCGGATAGCTTAACTGTTATGGAGCAAATGCCAGATAAGTG
TATAGATATAGCTGTTACTTCACCACCGTATAATCTTAAAAATTCGACAGGTAATGGTATGAAAGCCAATACCAAAAGTG
GTAAATGGGCAGGAAATGCACTACAAAATGGCTATAGCCACTATAACGATAATATTCCAAATGATGAATATGCTGAATGG
CAATACAATTGCTTGAAAGCAATGTATCGTCTTTTAAAAGACGATGGTGCTATTTTTTATAATCATAAATGGCGTGTGCA
AAATGGATTAATTCAAGACCGAACAGATATTATTAGAGATTTACCAGTTCGGCAAATTATTATATGGAAAAGAAAAGGTG
GTATTAATTTTAACCCGGGTTATTTTCTGCCTACCTATGAAGTTATTTATCTCATTGCAAAGCCAAGCTTTAAACTTCTA
CCTAAAGCTAATGCTTATGGAGATGTTTGGGAATTTACCCAAGAAATGAAAAATAATCATCCCGCACCATTTCCAGTAGC
TCTTATTGATAGAATTATTTCATCAACTTCGGCTCAAATCATTTTAGACCCTTTTATGGGTAGTGGTACAACTGCTGTTG
CAGCTTTACAGTTACAACGCAATTATATAGGTATTGATATTTCTCCTGATTATTGTGAGATGGCAAAAGAACGAATTTTA
AACTTGAATCCAGCTAAAAGATTTATTAAGAAAAATGGTTTAGAAACAATCTCTTTATTTGAAAAAATTGTATGA

Upstream 100 bases:

>100_bases
ATTCTAAACAATATCTTGAACAGCGCTCAAATTTTCGTTTACTTGCCAAGTGAATTTTTAAGGATATTTTTTCATTAAAA
GAAAAGCATTAAAAATCCGT

Downstream 100 bases:

>100_bases
AAATCTATACCAAAGAAGAACTAATCCAATCGTTGAAAATTATTGCAGCACAAGGATGGATAGAAAATGCGCGTCATGGT
AATCATGGTGGTATAGGTAA

Product: DNA modification methylase-like protein

Products: NA

Alternate protein names: M.BglII; N(4)- cytosine-specific methyltransferase BglII [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKFPDDYINTIICADSLTVMEQMPDKCIDIAVTSPPYNLKNSTGNGMKANTKSGKWAGNALQNGYSHYNDNIPNDEYAEW
QYNCLKAMYRLLKDDGAIFYNHKWRVQNGLIQDRTDIIRDLPVRQIIIWKRKGGINFNPGYFLPTYEVIYLIAKPSFKLL
PKANAYGDVWEFTQEMKNNHPAPFPVALIDRIISSTSAQIILDPFMGSGTTAVAALQLQRNYIGIDISPDYCEMAKERIL
NLNPAKRFIKKNGLETISLFEKIV

Sequences:

>Translated_264_residues
MKFPDDYINTIICADSLTVMEQMPDKCIDIAVTSPPYNLKNSTGNGMKANTKSGKWAGNALQNGYSHYNDNIPNDEYAEW
QYNCLKAMYRLLKDDGAIFYNHKWRVQNGLIQDRTDIIRDLPVRQIIIWKRKGGINFNPGYFLPTYEVIYLIAKPSFKLL
PKANAYGDVWEFTQEMKNNHPAPFPVALIDRIISSTSAQIILDPFMGSGTTAVAALQLQRNYIGIDISPDYCEMAKERIL
NLNPAKRFIKKNGLETISLFEKIV
>Mature_264_residues
MKFPDDYINTIICADSLTVMEQMPDKCIDIAVTSPPYNLKNSTGNGMKANTKSGKWAGNALQNGYSHYNDNIPNDEYAEW
QYNCLKAMYRLLKDDGAIFYNHKWRVQNGLIQDRTDIIRDLPVRQIIIWKRKGGINFNPGYFLPTYEVIYLIAKPSFKLL
PKANAYGDVWEFTQEMKNNHPAPFPVALIDRIISSTSAQIILDPFMGSGTTAVAALQLQRNYIGIDISPDYCEMAKERIL
NLNPAKRFIKKNGLETISLFEKIV

Specific function: This methylase recognizes the double-stranded sequence AGATCT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BglII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 30086; Mature: 30086

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFPDDYINTIICADSLTVMEQMPDKCIDIAVTSPPYNLKNSTGNGMKANTKSGKWAGNA
CCCCHHHHHHHHHCCHHHHHHHCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
LQNGYSHYNDNIPNDEYAEWQYNCLKAMYRLLKDDGAIFYNHKWRVQNGLIQDRTDIIRD
HHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECEEEECCCCHHHHHHHHHH
LPVRQIIIWKRKGGINFNPGYFLPTYEVIYLIAKPSFKLLPKANAYGDVWEFTQEMKNNH
CCCCEEEEEEECCCCCCCCCCCCCHHHEEEEEECCCEEECCCCCCCHHHHHHHHHHHCCC
PAPFPVALIDRIISSTSAQIILDPFMGSGTTAVAALQLQRNYIGIDISPDYCEMAKERIL
CCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHEEECCCEEEEECCCHHHHHHHHHHC
NLNPAKRFIKKNGLETISLFEKIV
CCCHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MKFPDDYINTIICADSLTVMEQMPDKCIDIAVTSPPYNLKNSTGNGMKANTKSGKWAGNA
CCCCHHHHHHHHHCCHHHHHHHCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
LQNGYSHYNDNIPNDEYAEWQYNCLKAMYRLLKDDGAIFYNHKWRVQNGLIQDRTDIIRD
HHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECEEEECCCCHHHHHHHHHH
LPVRQIIIWKRKGGINFNPGYFLPTYEVIYLIAKPSFKLLPKANAYGDVWEFTQEMKNNH
CCCCEEEEEEECCCCCCCCCCCCCHHHEEEEEECCCEEECCCCCCCHHHHHHHHHHHCCC
PAPFPVALIDRIISSTSAQIILDPFMGSGTTAVAALQLQRNYIGIDISPDYCEMAKERIL
CCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHEEECCCEEEEECCCHHHHHHHHHHC
NLNPAKRFIKKNGLETISLFEKIV
CCCHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9073062 [H]