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The map label for this gene is ispB [H]

Identifier: 78188931

GI number: 78188931

Start: 1301514

End: 1302488

Strand: Reverse

Name: ispB [H]

Synonym: Cag_0963

Alternate gene names: 78188931

Gene position: 1302488-1301514 (Counterclockwise)

Preceding gene: 78188933

Following gene: 78188930

Centisome position: 50.64

GC content: 45.54

Gene sequence:

>975_bases
GTGAATATTAAGGATGTTACGGCTTCAGTAGCCGATGAAATTGCCCTTTTTCAAGAAAAGTATCGCGTGGTGCTGCACTC
GCGCAACACGTTGGTTGATAAGGTGTCGCGCTATGTGCTCAAGCAACAAGGCAAGCAAATTCGCCCCGCTATTGTGTTGC
TTTCAGCAAACTTATGCGGCGGCGTTAATGAGTCAAGCTATCGAGCAGCAATTATGGTGGAGTTGCTCCATTCAGCCACG
CTTATTCACGACGATGTGGTGGATGGTGCTGAAATGCGACGTGGCTTACCTTCCATTAACGCTTTATGGAAAAATAAGAT
ATCGGTTTTAATGGGCGATTACATGCTGGCGCGTGGGTTGCTCTATTCCCTTGATCATAACGATTACGGTTTTTTGCACA
TGGTTTCCGAAGCGGTGCGCCGTATGAGTGAGGGCGAAATTCTACAAATTCAAAAAACCCGCAGCCTCGATATTACCGAA
AAGGATTACCTCAGCGTTATATCCGACAAAACTGCCTCGCTTATCTCCTCATCTTGTGCCATGGGTGCTATGAGTGCCAC
AACCGATGAAAGCAACATTGCCGCCTTAAAAAACTATGGCGAATATCTTGGGCTTGCCTTCCAAATCCGCGATGATTTAC
TTGATTACACGGGCGACTCTAAAACCACCGGCAAGCAAATGGGGATTGATATTAAGGATAAAAAAATCACCCTACCACTT
ATTTATGCCCTTTCGCAATCGTCAGCCACTGAGCAACGCCATATTCGTTCAATTTTAAAAAGTGCCCGTAAACGCGCCAT
TAAAAGTCCCGAAGTTATTGACTTTGTGCGCACAAAAGGTGGGCTTGAATATGCGGCAACGGTGGCAGAAAACTTTGCAA
GTAACGCAGTTCAATCCATTAGCCATTTTCCTGAAGGCAGTGCAAAAACCTCGCTGTTGCGGTTGGTGGATTTTGTAATG
ATGCGTCAATATTAA

Upstream 100 bases:

>100_bases
CTCTTACAATGGTACCCTAAAGAGGCTTTAAGGGCGTTGATTATAAAAAACGACCTTGAAGTTCCAACTTTTTTCCTGCA
CCCCTTTATCCATCTTATTT

Downstream 100 bases:

>100_bases
TGAAGCTGAGAGCAAGGTAAAAGGGGAATGGAAGTGTTAAAAAACTGTTGCTGTAACTCATGAAAAAAGCTGGACTTATC
ATTCTTGTTTTGTGTGGCAT

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MNIKDVTASVADEIALFQEKYRVVLHSRNTLVDKVSRYVLKQQGKQIRPAIVLLSANLCGGVNESSYRAAIMVELLHSAT
LIHDDVVDGAEMRRGLPSINALWKNKISVLMGDYMLARGLLYSLDHNDYGFLHMVSEAVRRMSEGEILQIQKTRSLDITE
KDYLSVISDKTASLISSSCAMGAMSATTDESNIAALKNYGEYLGLAFQIRDDLLDYTGDSKTTGKQMGIDIKDKKITLPL
IYALSQSSATEQRHIRSILKSARKRAIKSPEVIDFVRTKGGLEYAATVAENFASNAVQSISHFPEGSAKTSLLRLVDFVM
MRQY

Sequences:

>Translated_324_residues
MNIKDVTASVADEIALFQEKYRVVLHSRNTLVDKVSRYVLKQQGKQIRPAIVLLSANLCGGVNESSYRAAIMVELLHSAT
LIHDDVVDGAEMRRGLPSINALWKNKISVLMGDYMLARGLLYSLDHNDYGFLHMVSEAVRRMSEGEILQIQKTRSLDITE
KDYLSVISDKTASLISSSCAMGAMSATTDESNIAALKNYGEYLGLAFQIRDDLLDYTGDSKTTGKQMGIDIKDKKITLPL
IYALSQSSATEQRHIRSILKSARKRAIKSPEVIDFVRTKGGLEYAATVAENFASNAVQSISHFPEGSAKTSLLRLVDFVM
MRQY
>Mature_324_residues
MNIKDVTASVADEIALFQEKYRVVLHSRNTLVDKVSRYVLKQQGKQIRPAIVLLSANLCGGVNESSYRAAIMVELLHSAT
LIHDDVVDGAEMRRGLPSINALWKNKISVLMGDYMLARGLLYSLDHNDYGFLHMVSEAVRRMSEGEILQIQKTRSLDITE
KDYLSVISDKTASLISSSCAMGAMSATTDESNIAALKNYGEYLGLAFQIRDDLLDYTGDSKTTGKQMGIDIKDKKITLPL
IYALSQSSATEQRHIRSILKSARKRAIKSPEVIDFVRTKGGLEYAATVAENFASNAVQSISHFPEGSAKTSLLRLVDFVM
MRQY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=297, Percent_Identity=28.2828282828283, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4758430, Length=277, Percent_Identity=25.9927797833935, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI83700220, Length=277, Percent_Identity=25.9927797833935, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=32.9192546583851, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1786623, Length=261, Percent_Identity=29.8850574712644, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=311, Percent_Identity=28.2958199356913, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6319475, Length=308, Percent_Identity=28.2467532467532, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6325188, Length=258, Percent_Identity=23.2558139534884, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24651612, Length=324, Percent_Identity=28.0864197530864, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI281365769, Length=272, Percent_Identity=22.4264705882353, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24660002, Length=272, Percent_Identity=22.4264705882353, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35954; Mature: 35954

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKDVTASVADEIALFQEKYRVVLHSRNTLVDKVSRYVLKQQGKQIRPAIVLLSANLCG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEECHHCC
GVNESSYRAAIMVELLHSATLIHDDVVDGAEMRRGLPSINALWKNKISVLMGDYMLARGL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYSLDHNDYGFLHMVSEAVRRMSEGEILQIQKTRSLDITEKDYLSVISDKTASLISSSCA
HHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MGAMSATTDESNIAALKNYGEYLGLAFQIRDDLLDYTGDSKTTGKQMGIDIKDKKITLPL
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEECCCEEEHHH
IYALSQSSATEQRHIRSILKSARKRAIKSPEVIDFVRTKGGLEYAATVAENFASNAVQSI
HEEHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SHFPEGSAKTSLLRLVDFVMMRQY
HHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIKDVTASVADEIALFQEKYRVVLHSRNTLVDKVSRYVLKQQGKQIRPAIVLLSANLCG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEECHHCC
GVNESSYRAAIMVELLHSATLIHDDVVDGAEMRRGLPSINALWKNKISVLMGDYMLARGL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYSLDHNDYGFLHMVSEAVRRMSEGEILQIQKTRSLDITEKDYLSVISDKTASLISSSCA
HHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MGAMSATTDESNIAALKNYGEYLGLAFQIRDDLLDYTGDSKTTGKQMGIDIKDKKITLPL
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEECCCEEEHHH
IYALSQSSATEQRHIRSILKSARKRAIKSPEVIDFVRTKGGLEYAATVAENFASNAVQSI
HEEHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SHFPEGSAKTSLLRLVDFVMMRQY
HHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]