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The map label for this gene is nrfD [H]

Identifier: 78188596

GI number: 78188596

Start: 892192

End: 893184

Strand: Reverse

Name: nrfD [H]

Synonym: Cag_0618

Alternate gene names: 78188596

Gene position: 893184-892192 (Counterclockwise)

Preceding gene: 78188597

Following gene: 78188595

Centisome position: 34.73

GC content: 46.22

Gene sequence:

>993_bases
ATGATTGACGGCACTCGTGAACTCATTTCCACGAAACTTAATCCGCAGGTTTTACCGCACCTGCACATTTGGGAGTGGCA
CATTCCGCTCTACCTTTTTCTTGGCGGTATTGCTGGTGGCTTGCTGCTTATAACCTCAGTATTGATTGTTATGAAGCGCC
ACTTTGGCATTGGTCCGCGCGATGAAGGCGAAGGCTGCCCCTGCACCACCGTGCGCATTGGCGCTATTCTTTCGCCCGCA
CTCTTAGGCGTGGGAATGCTTTTTCTCTTTTTAGACCTTGCTCATAAGCTTTACGTATGGGCATTTTACACCACCATTCA
GCCCACCTCACCAATGTCGGCAGGAAGCTGGATTCTTATTGCTTTCTTTCCACTTGCTGTGCTGCAAGCTATGGTAGTAA
ACCGCAAATACCTTGAAAAGCTTAACATTGAGCTACTTAACAAGATCATTACGTGGACTGATGACCATTTGCTAAAAATA
GCCCTCATGAATGCACATATTGGGGTGGGAATTGGTATTTACACAGGTATTTTGCTCTCCTTCTTTTCAGCACGCCCATT
ATGGTCAAGCTCTATTCTTGGAATGCTCTTTTTAGTATCGGGCATTTCAAGCGCCGCTGCTTTAATGCTCTTGGTTGCTC
CAAAACATGAAAAGCACATTTACAGCCGCATTGATGCTAACGCCATGTGGATTGAGTTAGTAATTGTAGGACTTTTTATA
TTAGGCGGCATTACAGGTACCGAAAACACCCACGGCGCCATGATGCACCTTATTGCAGGCGAGCACACCATGGCAGGTAT
TCCCTACATGTACTGGTTTTGGGGAGTCTTTGTGTTTGCAGGTTTAGCGCTACCACTGCTCATTGAGTTCCTTGAAAATG
CAGGCTTCCACTTGCACATTCCACCAATTGCCCCCATGCTTGTGCTCATTGGTGGCTTAGTGCTACGCTTTTTGGTTGTG
TTTGCAGGACAAACTTACCACACTTTTATGTAA

Upstream 100 bases:

>100_bases
CTATGATGGCAGGTAAAGAGGTTTATCGCCAAAAAGCACATGCTGGCACAGAGCCACGACTTTACTGGATTTCCGCAAGT
AATAAACCCGGAGCGTAACT

Downstream 100 bases:

>100_bases
CGTTACATTTAACCTCGTTAAAGGCTTGTTTATCTGCTAAAAAAACAAAAGTAAACAAGCCTTTAACATCTATCCATTAT
CAGCTATGCAACAAGTTATT

Product: formate-dependent nitrite reductase membrane component-like protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MIDGTRELISTKLNPQVLPHLHIWEWHIPLYLFLGGIAGGLLLITSVLIVMKRHFGIGPRDEGEGCPCTTVRIGAILSPA
LLGVGMLFLFLDLAHKLYVWAFYTTIQPTSPMSAGSWILIAFFPLAVLQAMVVNRKYLEKLNIELLNKIITWTDDHLLKI
ALMNAHIGVGIGIYTGILLSFFSARPLWSSSILGMLFLVSGISSAAALMLLVAPKHEKHIYSRIDANAMWIELVIVGLFI
LGGITGTENTHGAMMHLIAGEHTMAGIPYMYWFWGVFVFAGLALPLLIEFLENAGFHLHIPPIAPMLVLIGGLVLRFLVV
FAGQTYHTFM

Sequences:

>Translated_330_residues
MIDGTRELISTKLNPQVLPHLHIWEWHIPLYLFLGGIAGGLLLITSVLIVMKRHFGIGPRDEGEGCPCTTVRIGAILSPA
LLGVGMLFLFLDLAHKLYVWAFYTTIQPTSPMSAGSWILIAFFPLAVLQAMVVNRKYLEKLNIELLNKIITWTDDHLLKI
ALMNAHIGVGIGIYTGILLSFFSARPLWSSSILGMLFLVSGISSAAALMLLVAPKHEKHIYSRIDANAMWIELVIVGLFI
LGGITGTENTHGAMMHLIAGEHTMAGIPYMYWFWGVFVFAGLALPLLIEFLENAGFHLHIPPIAPMLVLIGGLVLRFLVV
FAGQTYHTFM
>Mature_330_residues
MIDGTRELISTKLNPQVLPHLHIWEWHIPLYLFLGGIAGGLLLITSVLIVMKRHFGIGPRDEGEGCPCTTVRIGAILSPA
LLGVGMLFLFLDLAHKLYVWAFYTTIQPTSPMSAGSWILIAFFPLAVLQAMVVNRKYLEKLNIELLNKIITWTDDHLLKI
ALMNAHIGVGIGIYTGILLSFFSARPLWSSSILGMLFLVSGISSAAALMLLVAPKHEKHIYSRIDANAMWIELVIVGLFI
LGGITGTENTHGAMMHLIAGEHTMAGIPYMYWFWGVFVFAGLALPLLIEFLENAGFHLHIPPIAPMLVLIGGLVLRFLVV
FAGQTYHTFM

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=318, Percent_Identity=27.0440251572327, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 36452; Mature: 36452

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDGTRELISTKLNPQVLPHLHIWEWHIPLYLFLGGIAGGLLLITSVLIVMKRHFGIGPR
CCCCHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DEGEGCPCTTVRIGAILSPALLGVGMLFLFLDLAHKLYVWAFYTTIQPTSPMSAGSWILI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHH
AFFPLAVLQAMVVNRKYLEKLNIELLNKIITWTDDHLLKIALMNAHIGVGIGIYTGILLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHEEEHHHHCCHHHHHHHHHHH
FFSARPLWSSSILGMLFLVSGISSAAALMLLVAPKHEKHIYSRIDANAMWIELVIVGLFI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
LGGITGTENTHGAMMHLIAGEHTMAGIPYMYWFWGVFVFAGLALPLLIEFLENAGFHLHI
HCCCCCCCCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PPIAPMLVLIGGLVLRFLVVFAGQTYHTFM
CCHHHHHHHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MIDGTRELISTKLNPQVLPHLHIWEWHIPLYLFLGGIAGGLLLITSVLIVMKRHFGIGPR
CCCCHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DEGEGCPCTTVRIGAILSPALLGVGMLFLFLDLAHKLYVWAFYTTIQPTSPMSAGSWILI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHH
AFFPLAVLQAMVVNRKYLEKLNIELLNKIITWTDDHLLKIALMNAHIGVGIGIYTGILLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHEEEHHHHCCHHHHHHHHHHH
FFSARPLWSSSILGMLFLVSGISSAAALMLLVAPKHEKHIYSRIDANAMWIELVIVGLFI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
LGGITGTENTHGAMMHLIAGEHTMAGIPYMYWFWGVFVFAGLALPLLIEFLENAGFHLHI
HCCCCCCCCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PPIAPMLVLIGGLVLRFLVVFAGQTYHTFM
CCHHHHHHHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]