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The map label for this gene is mrcA [H]

Identifier: 78188568

GI number: 78188568

Start: 692934

End: 695222

Strand: Reverse

Name: mrcA [H]

Synonym: Cag_0590

Alternate gene names: 78188568

Gene position: 695222-692934 (Counterclockwise)

Preceding gene: 78188569

Following gene: 78188567

Centisome position: 27.03

GC content: 47.88

Gene sequence:

>2289_bases
GTGAAATCACTGCTTTATAAATCGCTCTTTCTTTTGCTTGCCTATGTTTTGAGCGTCAGTGCAACGGCACCCTCAGCCTA
TGCTATGCGTTCGCTTTTGGGGCTACCAAGCGTTGAAGAGCTTGAAAATCCCAATCCCGAGCTTGCCTCATTAGTCTATT
CTGAGGATGGCGTGCTTATTCATAAGTACTTCAATAAAAACCGCACCTTCGTGCCATTACGCTCTATTCCTCGCTCAACT
CGTTATGCCCTTATTGCTACCGAAGATGCTGAGTTTTACAACCATTGGGGCGTTAACGTGCGCCGTGTTTTTGTAGCAAT
GGGCGAAAATCTTTTTCGAGCACCAAAACGGTGGCATGGTGCCAGCACTATTACGCAGCAGCTTGCTAAAAACCTCTACT
TAACGCAAGAACGCACCTTTTCCCGCAAGTTTAAGGAGCTGATTACGGCTATTGAGCTGGAACGCACTTATACCAAAGAT
GAAATTTTAGCGCTCTATTTTAACACGGTCTATTTTGGTGCGGGTGCCTATGGCATTGAATCGGCAGCTCAAACCTATTT
TGGTAAATCGGCTTCGCAGCTTACCCTGCCCGAAAGCGCAACGCTGATTGCTACCCTTAAAAATCCCACAGCTTATAATC
CTGCAAAAAATCCCGCAGGCTCTATAAGCCGCCGCAACTTGATTTTGGGATTAATGGAGAAAAATAAGTTTATCACTCCT
CAGCAAGCCGCAAAAGCTAAACGTACCCCGCTAACGCTGAAATACACGCCGTTAAACCAGCAAGGGTTAGCGCCATATTT
TGCCGAGTACATTCGCCAAACCATTAAACCAGCAACTATTTTAGGCGATTTAAATCTTTATCGCGATGGATTAACGGTGC
GCACAACGCTTGATAGCCGAATGCAAAAATATGCGCAACAAGCGGCAGTGGAACACCTTGCTTCGTTACAAGCGGCATTT
GACCGTTCATGGCGCTGGCCTGAAAATTTGAAAAATCAGATAATTCGCGAAAGTGAGCGTTATAAAGAGTTAGTTGGTAG
TGGCATGAGCGACGGGCAAGCTATGGCGCGTTTAAAGGCTGATAATGTATGGTTACATAATATTTTACGTGAGAAAACTC
GTATTCAAGTGGCGTTGGTAGCTATTGACCCTAACAACGGACACGTTAAAGCATGGGTTGGTGGAAATAGCCTTTCGCCC
GATGAGTACAAATATCAATTTGACCATGTGTGGCAAGCGCGTCGCCAGCCGGGTTCTACCTTTAAGCCATTTGTTTACAC
GGCGGCTATTGATAAAGGGCTACCCGCCAATTTTCAAGTGCTTGACCAGCCATTGCAACTCAGTAGTGGCGATGGCATTT
GGTCGCCCCGCAACTCCGATGGCTCTTCGGGCGGCATGACTACCCTTCGCTCAGCCCTCACCCGTTCGCTGAACCAAGTA
ACAGTGCGCCTTGCTTACGAGCACCTTAGCCCTGCCGAAATTATTAGCTATGCAAAGCGCATGGGCATTAATTCCCCCAT
GCCTAACGATTTATCAATTGCGCTGGGCACGGCAGCAGTCTCACCGCTTGAGCTTGCGGGCGCATTCACTCCATTTGCTA
ATAATGGAATATGGAGTGAACCTATCTCCATTTTAAAAGTGGAAGACAAGCACAAGCGCTTTATTACCAGCCAAAAGCCG
AATAGCCGCTTTGCAATTGATTCCACCACCAACTATGTCATGGTTTCCATGTTGCGCGATGTTATTAATCGGGGCACAGG
TGCCTCAGTACGGAGCTACGGCTTTACGGCTGAAGCGGCAGGAAAAACAGGTACAACGCAAAACATGAAGGATGCGTGGT
TTGCGGGCTTTACCCCACAACTTGTGGCTGTTGTGTGGACAGGCTTTGATGATGAACGCATTAAGTTTACATCAATGGAA
TATGGGCAGGGTGCCCGTGCTGCTCTCCCAATTTGGGCAAAATTCATGCAGCGCTGCTACAGCGACCCAACGCTCAAGCT
TGGCAGCCGCTACTTCCACATTCCCGAAACGGTTATTGCGGTACCAACCTCATCCGCCCAAAACAATATGGCTGCCGATT
TGCTTGGCGGCAACGTAAGCTTTGAATACTTCACCCCAAAAGGCTTTGAATATTATCAATCGCATCCCGAATTGGCAATA
AGTAGCCCACCACCTATGGCTCCAATTGATAGCAGTAGCAATGGCGTAAGTGCCGTAACCATGCCTGCACTCAAGCCCGT
TGCACCCGTACCATCGGCAGCAAAGCCAAAAGTGGAGAAGCCTCACTAA

Upstream 100 bases:

>100_bases
TGTTTGCTCAAGTGCTTGATAAACATTTTCAACAGGCTCAACAGTTGCTCTAAACTGTTAAGTTGACCGTAACATTTTCA
CAACAAGACATTCACCTATC

Downstream 100 bases:

>100_bases
CAACTTTTTATAGCGCCACAAACAAGCACGAACGAGCTTGGCGTGGCGCTCCACATTTTCTAACCCTAACACTCACCTCC
TATGCAATCAGTTTTAGTGC

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 762; Mature: 762

Protein sequence:

>762_residues
MKSLLYKSLFLLLAYVLSVSATAPSAYAMRSLLGLPSVEELENPNPELASLVYSEDGVLIHKYFNKNRTFVPLRSIPRST
RYALIATEDAEFYNHWGVNVRRVFVAMGENLFRAPKRWHGASTITQQLAKNLYLTQERTFSRKFKELITAIELERTYTKD
EILALYFNTVYFGAGAYGIESAAQTYFGKSASQLTLPESATLIATLKNPTAYNPAKNPAGSISRRNLILGLMEKNKFITP
QQAAKAKRTPLTLKYTPLNQQGLAPYFAEYIRQTIKPATILGDLNLYRDGLTVRTTLDSRMQKYAQQAAVEHLASLQAAF
DRSWRWPENLKNQIIRESERYKELVGSGMSDGQAMARLKADNVWLHNILREKTRIQVALVAIDPNNGHVKAWVGGNSLSP
DEYKYQFDHVWQARRQPGSTFKPFVYTAAIDKGLPANFQVLDQPLQLSSGDGIWSPRNSDGSSGGMTTLRSALTRSLNQV
TVRLAYEHLSPAEIISYAKRMGINSPMPNDLSIALGTAAVSPLELAGAFTPFANNGIWSEPISILKVEDKHKRFITSQKP
NSRFAIDSTTNYVMVSMLRDVINRGTGASVRSYGFTAEAAGKTGTTQNMKDAWFAGFTPQLVAVVWTGFDDERIKFTSME
YGQGARAALPIWAKFMQRCYSDPTLKLGSRYFHIPETVIAVPTSSAQNNMAADLLGGNVSFEYFTPKGFEYYQSHPELAI
SSPPPMAPIDSSSNGVSAVTMPALKPVAPVPSAAKPKVEKPH

Sequences:

>Translated_762_residues
MKSLLYKSLFLLLAYVLSVSATAPSAYAMRSLLGLPSVEELENPNPELASLVYSEDGVLIHKYFNKNRTFVPLRSIPRST
RYALIATEDAEFYNHWGVNVRRVFVAMGENLFRAPKRWHGASTITQQLAKNLYLTQERTFSRKFKELITAIELERTYTKD
EILALYFNTVYFGAGAYGIESAAQTYFGKSASQLTLPESATLIATLKNPTAYNPAKNPAGSISRRNLILGLMEKNKFITP
QQAAKAKRTPLTLKYTPLNQQGLAPYFAEYIRQTIKPATILGDLNLYRDGLTVRTTLDSRMQKYAQQAAVEHLASLQAAF
DRSWRWPENLKNQIIRESERYKELVGSGMSDGQAMARLKADNVWLHNILREKTRIQVALVAIDPNNGHVKAWVGGNSLSP
DEYKYQFDHVWQARRQPGSTFKPFVYTAAIDKGLPANFQVLDQPLQLSSGDGIWSPRNSDGSSGGMTTLRSALTRSLNQV
TVRLAYEHLSPAEIISYAKRMGINSPMPNDLSIALGTAAVSPLELAGAFTPFANNGIWSEPISILKVEDKHKRFITSQKP
NSRFAIDSTTNYVMVSMLRDVINRGTGASVRSYGFTAEAAGKTGTTQNMKDAWFAGFTPQLVAVVWTGFDDERIKFTSME
YGQGARAALPIWAKFMQRCYSDPTLKLGSRYFHIPETVIAVPTSSAQNNMAADLLGGNVSFEYFTPKGFEYYQSHPELAI
SSPPPMAPIDSSSNGVSAVTMPALKPVAPVPSAAKPKVEKPH
>Mature_762_residues
MKSLLYKSLFLLLAYVLSVSATAPSAYAMRSLLGLPSVEELENPNPELASLVYSEDGVLIHKYFNKNRTFVPLRSIPRST
RYALIATEDAEFYNHWGVNVRRVFVAMGENLFRAPKRWHGASTITQQLAKNLYLTQERTFSRKFKELITAIELERTYTKD
EILALYFNTVYFGAGAYGIESAAQTYFGKSASQLTLPESATLIATLKNPTAYNPAKNPAGSISRRNLILGLMEKNKFITP
QQAAKAKRTPLTLKYTPLNQQGLAPYFAEYIRQTIKPATILGDLNLYRDGLTVRTTLDSRMQKYAQQAAVEHLASLQAAF
DRSWRWPENLKNQIIRESERYKELVGSGMSDGQAMARLKADNVWLHNILREKTRIQVALVAIDPNNGHVKAWVGGNSLSP
DEYKYQFDHVWQARRQPGSTFKPFVYTAAIDKGLPANFQVLDQPLQLSSGDGIWSPRNSDGSSGGMTTLRSALTRSLNQV
TVRLAYEHLSPAEIISYAKRMGINSPMPNDLSIALGTAAVSPLELAGAFTPFANNGIWSEPISILKVEDKHKRFITSQKP
NSRFAIDSTTNYVMVSMLRDVINRGTGASVRSYGFTAEAAGKTGTTQNMKDAWFAGFTPQLVAVVWTGFDDERIKFTSME
YGQGARAALPIWAKFMQRCYSDPTLKLGSRYFHIPETVIAVPTSSAQNNMAADLLGGNVSFEYFTPKGFEYYQSHPELAI
SSPPPMAPIDSSSNGVSAVTMPALKPVAPVPSAAKPKVEKPH

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=627, Percent_Identity=32.5358851674641, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1786343, Length=631, Percent_Identity=29.9524564183835, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1788867, Length=616, Percent_Identity=26.461038961039, Blast_Score=150, Evalue=4e-37,
Organism=Escherichia coli, GI1789601, Length=192, Percent_Identity=38.0208333333333, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 84548; Mature: 84548

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLLYKSLFLLLAYVLSVSATAPSAYAMRSLLGLPSVEELENPNPELASLVYSEDGVLI
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHCCCCHHHHHEEECCCCEEE
HKYFNKNRTFVPLRSIPRSTRYALIATEDAEFYNHWGVNVRRVFVAMGENLFRAPKRWHG
EEECCCCCCEEEHHCCCCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCC
ASTITQQLAKNLYLTQERTFSRKFKELITAIELERTYTKDEILALYFNTVYFGAGAYGIE
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEHHHHCCCCCHHHH
SAAQTYFGKSASQLTLPESATLIATLKNPTAYNPAKNPAGSISRRNLILGLMEKNKFITP
HHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCH
QQAAKAKRTPLTLKYTPLNQQGLAPYFAEYIRQTIKPATILGDLNLYRDGLTVRTTLDSR
HHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHCCCEEEEHHHHH
MQKYAQQAAVEHLASLQAAFDRSWRWPENLKNQIIRESERYKELVGSGMSDGQAMARLKA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
DNVWLHNILREKTRIQVALVAIDPNNGHVKAWVGGNSLSPDEYKYQFDHVWQARRQPGST
CCHHHHHHHHHCCEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
FKPFVYTAAIDKGLPANFQVLDQPLQLSSGDGIWSPRNSDGSSGGMTTLRSALTRSLNQV
CCCEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TVRLAYEHLSPAEIISYAKRMGINSPMPNDLSIALGTAAVSPLELAGAFTPFANNGIWSE
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHCCHHHHHCCCCCCCCCCCCCC
PISILKVEDKHKRFITSQKPNSRFAIDSTTNYVMVSMLRDVINRGTGASVRSYGFTAEAA
CEEEEEECCHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHHCCCCHHCC
GKTGTTQNMKDAWFAGFTPQLVAVVWTGFDDERIKFTSMEYGQGARAALPIWAKFMQRCY
CCCCCCCCCCHHHHCCCCHHEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHC
SDPTLKLGSRYFHIPETVIAVPTSSAQNNMAADLLGGNVSFEYFTPKGFEYYQSHPELAI
CCCCEECCCEEEECCCEEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCHHHHCCCCEEE
SSPPPMAPIDSSSNGVSAVTMPALKPVAPVPSAAKPKVEKPH
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSLLYKSLFLLLAYVLSVSATAPSAYAMRSLLGLPSVEELENPNPELASLVYSEDGVLI
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHCCCCHHHHHEEECCCCEEE
HKYFNKNRTFVPLRSIPRSTRYALIATEDAEFYNHWGVNVRRVFVAMGENLFRAPKRWHG
EEECCCCCCEEEHHCCCCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCC
ASTITQQLAKNLYLTQERTFSRKFKELITAIELERTYTKDEILALYFNTVYFGAGAYGIE
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEHHHHCCCCCHHHH
SAAQTYFGKSASQLTLPESATLIATLKNPTAYNPAKNPAGSISRRNLILGLMEKNKFITP
HHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCH
QQAAKAKRTPLTLKYTPLNQQGLAPYFAEYIRQTIKPATILGDLNLYRDGLTVRTTLDSR
HHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHCCCEEEEHHHHH
MQKYAQQAAVEHLASLQAAFDRSWRWPENLKNQIIRESERYKELVGSGMSDGQAMARLKA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
DNVWLHNILREKTRIQVALVAIDPNNGHVKAWVGGNSLSPDEYKYQFDHVWQARRQPGST
CCHHHHHHHHHCCEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
FKPFVYTAAIDKGLPANFQVLDQPLQLSSGDGIWSPRNSDGSSGGMTTLRSALTRSLNQV
CCCEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TVRLAYEHLSPAEIISYAKRMGINSPMPNDLSIALGTAAVSPLELAGAFTPFANNGIWSE
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHCCHHHHHCCCCCCCCCCCCCC
PISILKVEDKHKRFITSQKPNSRFAIDSTTNYVMVSMLRDVINRGTGASVRSYGFTAEAA
CEEEEEECCHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHHCCCCHHCC
GKTGTTQNMKDAWFAGFTPQLVAVVWTGFDDERIKFTSMEYGQGARAALPIWAKFMQRCY
CCCCCCCCCCHHHHCCCCHHEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHC
SDPTLKLGSRYFHIPETVIAVPTSSAQNNMAADLLGGNVSFEYFTPKGFEYYQSHPELAI
CCCCEECCCEEEECCCEEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCHHHHCCCCEEE
SSPPPMAPIDSSSNGVSAVTMPALKPVAPVPSAAKPKVEKPH
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA