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The map label for this gene is ppdK [H]

Identifier: 78188138

GI number: 78188138

Start: 174339

End: 177089

Strand: Reverse

Name: ppdK [H]

Synonym: Cag_0155

Alternate gene names: 78188138

Gene position: 177089-174339 (Counterclockwise)

Preceding gene: 78188140

Following gene: 78188137

Centisome position: 6.89

GC content: 46.56

Gene sequence:

>2751_bases
ATGCCAAATCCAGAGAAGACCAACGAACAGGCTGCAAAAAAACAGTACCTCTACAGTTTTGCGGGCGGTGCCTCCGACGG
TGACGCCTCCATGAAAAATCTGCTTGGTGGCAAAGGCGCAAACCTTGCAGAAATGGCAAACATAGGACTGCCAGTTCCTC
CAGGATTCACCATCTCCACCGAAGTCTGCACTTACTACTACGATAATCAGAAAAACTATCCACCCAACCTTTTTGAGCAA
GATATTCCTGACGCTCTCAGTAAAATTGAATATATGCTTGGCAAAAAATTTGGTGACCCCGTAAATCCGCTACTTGTTTC
TGTTCGCTCCGGTGCTCGTGCTTCAATGCCCGGCATGATGGATACCATTCTTAACCTCGGTTTAAACTCCCAAACAGTTG
ATGGACTTGCTAAAAGATCGGGTAACCCCCGTTTTGCTTGGGATTGCTACCGTCGTTTTGTACAAATGTATGGCGATGTA
GTGCTCGATTTAAAGCCTACCAATAAAAAAGAGATTGACCCCTTTGAAAAAATTCTTGAAGAGAAAAAGCATGAGCGTGG
CATTACGCTCGACACTGAGTTTACGGTTGAAGATTTACAAGACCTTGTAAGCCGCTACAAAGCCGCTATTCTTGAAAAAA
CGGGCAGAACCTTCCCTGAAGATCCACACGAACAACTTCGTGGAGCTATTGGCGCTGTATTTGGCAGTTGGAACAACGAG
CGTGCTATTATTTACCGCAAGCTCAACCACATTCCCGGTTATTGGGGTACCGCTTGTAACGTACAAGCTATGGTGTTTGG
TAACATGGGTGAAGATTGCGGTACGGGCGTTGCCTTTACGCGCGATGCCGCAACCGGCGAAAACATTTTCTACGGTGAAT
TCTTAATGAATGCGCAAGGTGAAGATGTGGTTGCAGGTACCAGAACCCCGCTGAAAATTGAGCAACTTAAAGCCAACAAG
CCTGATATTTATGACCAACTTGAAGAGATTCGTTCCTCTCTTGAGCGCCACTATCGCGATATGATGGATATTGAGTTCAC
CATTGAAAGCAATACGCTCTTTATGTTGCAATGCCGTGTTGGTAAGCGCACAGGTATGGCTGCTGTTAAGATTGCTGTTG
ATATGTTTAACGAAGGGCTTATTGATGAAAAAGAGGCTCTTATGCGTATTGAGCCGGAACAACTCAACCAGTTGCTTCGC
CCTGTCTTTAATATGAAGGAAAAACAAGCGGCTATTAGCGGTGGACGTTTGCTTGCAACCGGTTTAAATGCAGGTCCGGG
TGCTGCTACGGGTAAAATTTACTTCAACGCTGTTGATGCCTACGAGGCAAGCAAAAAAGGCGAGGCGGTTGTTTTAGTTC
GTATTGAAACTTCCCCTGAGGATATTAAAGGTATGGCGGTTGCTGAAGGTATTCTTACCGAACGTGGTGGCATGACCTCA
CACGCCGCTCTTGTGGCTCGCCAAATGGGTAAGGTGTGCGTTGCTGGTTGTGGTGCCTTGCAAATTGATTATCAAAAAGG
TGAAATGCGCGTTGCAGGCAAAGATATTGTGCTCAGCGAAGGCGACTACATTTCAATTGATGGCAGCACGGGTGAAGTTA
TTGCTGGCAAGGTTGCAACCAAAAACTCCGAGATTCTTGAGGTGCTGATTGATAAAACCTTAAAGCCTGAAGATGCTTCC
ACATGGCCTATTTACAAGCAATTAATGAAGTGGGCTGATAAATATCGTAAGCTAAAAATTCGTACCAACGCTGACCAACC
AGATCAAGCCGATATTGCAGTAACCTTTGGCGCTGAAGGTATTGGGCTCTGCCGCACCGAGCACATGTTCTTTGGCGGCG
AGCGTATTGATGCAATGCGCGAAATGATTCTTGCTGATGATATTACCGGACGCCAAAAAGCACTTGAAAAACTACTCCCT
TACCAACGCGAAGACTTTTACGGACTCTTTAAGTGCATGGGCGAACGTCCTGTAACCATCCGCTTGCTTGATCCACCGCT
TCATGAGTTCCTCCCTCACACCGATGCTGAAATTACGGCACTTGCTGCTAAAATTGGCAAGAGCTATGAAGAGGTGAACA
TGCGTATTGAGCACTTGCATGAGTTTAACCCCATGCTTGGCTTGCGTGGTTGCCGCCTTGGCATTCTCCACCCCGAAATT
GCGGTTATGCAGGTTCGCGCTATTATTGAAGCTGCTTGCCAATTGAAAAAAGAGGGGCTGGAAGTAATTCCCGAAATTAT
GGTACCGCTTGTTAGCACGGTTAAAGAGCTTGAAATCACCAGTGAAATTATTCACAAAACCGCTCGCAGCGTTTTTGCCG
AACAAGGCATTGGGGTGCAGTACTTGGTTGGCACCATGATTGAAGTGCCTCGTGCCGCCATTACCTCCGACCAAATTGCA
ACGGTTGCCGACTTCTTTAGCTACGGCACCAACGACTTAACACAAATGGGACTTGGTATGAGCCGCGACGATTCAGGGCA
GTTCCTCCCAACCTATCAGCAGCAAGAAATTTTTGCACGCAACCCATTTGAATCATTGGATATTGATGGTGTAGGACGCT
TAATGAGCATTTCAGCAAAAGATGGACGTGCTGTTAAAGCTGACTTAAAGCTTGGTATTTGTGGTGAACATGGTGGCGAT
CCTGCAACCGTTGAGTTCTGCCATAAACTTGGCTTGAACTATGTTTCATGCAGTCCATTCCGTGTACCAATTGCTCGCCT
TGCCGCCGCAAGAGCAGCATTGCAAGATTAA

Upstream 100 bases:

>100_bases
ACCACTATGGGAACGCACGTTTTTGCCGTGCGCTCGCCTATTTTTCTTTTTTTATAGGTAAAAACAATAGGTGTATCAAC
ATAATTTATTCCCGAACATC

Downstream 100 bases:

>100_bases
ATTTTTGCATAATTCCATTGTGAAAAAGGCGGCGATAAGTCGCCTTTTTCATTAAACAGAGCTATCTCTCTAAAAGGTAA
TGGCATAGCAATACAATTAT

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 916; Mature: 915

Protein sequence:

>916_residues
MPNPEKTNEQAAKKQYLYSFAGGASDGDASMKNLLGGKGANLAEMANIGLPVPPGFTISTEVCTYYYDNQKNYPPNLFEQ
DIPDALSKIEYMLGKKFGDPVNPLLVSVRSGARASMPGMMDTILNLGLNSQTVDGLAKRSGNPRFAWDCYRRFVQMYGDV
VLDLKPTNKKEIDPFEKILEEKKHERGITLDTEFTVEDLQDLVSRYKAAILEKTGRTFPEDPHEQLRGAIGAVFGSWNNE
RAIIYRKLNHIPGYWGTACNVQAMVFGNMGEDCGTGVAFTRDAATGENIFYGEFLMNAQGEDVVAGTRTPLKIEQLKANK
PDIYDQLEEIRSSLERHYRDMMDIEFTIESNTLFMLQCRVGKRTGMAAVKIAVDMFNEGLIDEKEALMRIEPEQLNQLLR
PVFNMKEKQAAISGGRLLATGLNAGPGAATGKIYFNAVDAYEASKKGEAVVLVRIETSPEDIKGMAVAEGILTERGGMTS
HAALVARQMGKVCVAGCGALQIDYQKGEMRVAGKDIVLSEGDYISIDGSTGEVIAGKVATKNSEILEVLIDKTLKPEDAS
TWPIYKQLMKWADKYRKLKIRTNADQPDQADIAVTFGAEGIGLCRTEHMFFGGERIDAMREMILADDITGRQKALEKLLP
YQREDFYGLFKCMGERPVTIRLLDPPLHEFLPHTDAEITALAAKIGKSYEEVNMRIEHLHEFNPMLGLRGCRLGILHPEI
AVMQVRAIIEAACQLKKEGLEVIPEIMVPLVSTVKELEITSEIIHKTARSVFAEQGIGVQYLVGTMIEVPRAAITSDQIA
TVADFFSYGTNDLTQMGLGMSRDDSGQFLPTYQQQEIFARNPFESLDIDGVGRLMSISAKDGRAVKADLKLGICGEHGGD
PATVEFCHKLGLNYVSCSPFRVPIARLAAARAALQD

Sequences:

>Translated_916_residues
MPNPEKTNEQAAKKQYLYSFAGGASDGDASMKNLLGGKGANLAEMANIGLPVPPGFTISTEVCTYYYDNQKNYPPNLFEQ
DIPDALSKIEYMLGKKFGDPVNPLLVSVRSGARASMPGMMDTILNLGLNSQTVDGLAKRSGNPRFAWDCYRRFVQMYGDV
VLDLKPTNKKEIDPFEKILEEKKHERGITLDTEFTVEDLQDLVSRYKAAILEKTGRTFPEDPHEQLRGAIGAVFGSWNNE
RAIIYRKLNHIPGYWGTACNVQAMVFGNMGEDCGTGVAFTRDAATGENIFYGEFLMNAQGEDVVAGTRTPLKIEQLKANK
PDIYDQLEEIRSSLERHYRDMMDIEFTIESNTLFMLQCRVGKRTGMAAVKIAVDMFNEGLIDEKEALMRIEPEQLNQLLR
PVFNMKEKQAAISGGRLLATGLNAGPGAATGKIYFNAVDAYEASKKGEAVVLVRIETSPEDIKGMAVAEGILTERGGMTS
HAALVARQMGKVCVAGCGALQIDYQKGEMRVAGKDIVLSEGDYISIDGSTGEVIAGKVATKNSEILEVLIDKTLKPEDAS
TWPIYKQLMKWADKYRKLKIRTNADQPDQADIAVTFGAEGIGLCRTEHMFFGGERIDAMREMILADDITGRQKALEKLLP
YQREDFYGLFKCMGERPVTIRLLDPPLHEFLPHTDAEITALAAKIGKSYEEVNMRIEHLHEFNPMLGLRGCRLGILHPEI
AVMQVRAIIEAACQLKKEGLEVIPEIMVPLVSTVKELEITSEIIHKTARSVFAEQGIGVQYLVGTMIEVPRAAITSDQIA
TVADFFSYGTNDLTQMGLGMSRDDSGQFLPTYQQQEIFARNPFESLDIDGVGRLMSISAKDGRAVKADLKLGICGEHGGD
PATVEFCHKLGLNYVSCSPFRVPIARLAAARAALQD
>Mature_915_residues
PNPEKTNEQAAKKQYLYSFAGGASDGDASMKNLLGGKGANLAEMANIGLPVPPGFTISTEVCTYYYDNQKNYPPNLFEQD
IPDALSKIEYMLGKKFGDPVNPLLVSVRSGARASMPGMMDTILNLGLNSQTVDGLAKRSGNPRFAWDCYRRFVQMYGDVV
LDLKPTNKKEIDPFEKILEEKKHERGITLDTEFTVEDLQDLVSRYKAAILEKTGRTFPEDPHEQLRGAIGAVFGSWNNER
AIIYRKLNHIPGYWGTACNVQAMVFGNMGEDCGTGVAFTRDAATGENIFYGEFLMNAQGEDVVAGTRTPLKIEQLKANKP
DIYDQLEEIRSSLERHYRDMMDIEFTIESNTLFMLQCRVGKRTGMAAVKIAVDMFNEGLIDEKEALMRIEPEQLNQLLRP
VFNMKEKQAAISGGRLLATGLNAGPGAATGKIYFNAVDAYEASKKGEAVVLVRIETSPEDIKGMAVAEGILTERGGMTSH
AALVARQMGKVCVAGCGALQIDYQKGEMRVAGKDIVLSEGDYISIDGSTGEVIAGKVATKNSEILEVLIDKTLKPEDAST
WPIYKQLMKWADKYRKLKIRTNADQPDQADIAVTFGAEGIGLCRTEHMFFGGERIDAMREMILADDITGRQKALEKLLPY
QREDFYGLFKCMGERPVTIRLLDPPLHEFLPHTDAEITALAAKIGKSYEEVNMRIEHLHEFNPMLGLRGCRLGILHPEIA
VMQVRAIIEAACQLKKEGLEVIPEIMVPLVSTVKELEITSEIIHKTARSVFAEQGIGVQYLVGTMIEVPRAAITSDQIAT
VADFFSYGTNDLTQMGLGMSRDDSGQFLPTYQQQEIFARNPFESLDIDGVGRLMSISAKDGRAVKADLKLGICGEHGGDP
ATVEFCHKLGLNYVSCSPFRVPIARLAAARAALQD

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=717, Percent_Identity=25.5230125523013, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789193, Length=446, Percent_Identity=24.6636771300448, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788756, Length=462, Percent_Identity=22.5108225108225, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI48994992, Length=451, Percent_Identity=24.390243902439, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 101069; Mature: 100938

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNPEKTNEQAAKKQYLYSFAGGASDGDASMKNLLGGKGANLAEMANIGLPVPPGFTIST
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEH
EVCTYYYDNQKNYPPNLFEQDIPDALSKIEYMLGKKFGDPVNPLLVSVRSGARASMPGMM
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCCCHHH
DTILNLGLNSQTVDGLAKRSGNPRFAWDCYRRFVQMYGDVVLDLKPTNKKEIDPFEKILE
HHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHH
EKKHERGITLDTEFTVEDLQDLVSRYKAAILEKTGRTFPEDPHEQLRGAIGAVFGSWNNE
HHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
RAIIYRKLNHIPGYWGTACNVQAMVFGNMGEDCGTGVAFTRDAATGENIFYGEFLMNAQG
CEEEEEEHHCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCCCEEEEHEEECCCC
EDVVAGTRTPLKIEQLKANKPDIYDQLEEIRSSLERHYRDMMDIEFTIESNTLFMLQCRV
CCEEECCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEEEC
GKRTGMAAVKIAVDMFNEGLIDEKEALMRIEPEQLNQLLRPVFNMKEKQAAISGGRLLAT
CCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCEEEEE
GLNAGPGAATGKIYFNAVDAYEASKKGEAVVLVRIETSPEDIKGMAVAEGILTERGGMTS
CCCCCCCCCCCEEEEEEEHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCHH
HAALVARQMGKVCVAGCGALQIDYQKGEMRVAGKDIVLSEGDYISIDGSTGEVIAGKVAT
HHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCEEEEECCCEEEECCCCCCEEEEEEEC
KNSEILEVLIDKTLKPEDASTWPIYKQLMKWADKYRKLKIRTNADQPDQADIAVTFGAEG
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEECCCC
IGLCRTEHMFFGGERIDAMREMILADDITGRQKALEKLLPYQREDFYGLFKCMGERPVTI
CCEEEECCEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCEEE
RLLDPPLHEFLPHTDAEITALAAKIGKSYEEVNMRIEHLHEFNPMLGLRGCRLGILHPEI
EEECCCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHH
AVMQVRAIIEAACQLKKEGLEVIPEIMVPLVSTVKELEITSEIIHKTARSVFAEQGIGVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
YLVGTMIEVPRAAITSDQIATVADFFSYGTNDLTQMGLGMSRDDSGQFLPTYQQQEIFAR
HHHHHHHHCCHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHC
NPFESLDIDGVGRLMSISAKDGRAVKADLKLGICGEHGGDPATVEFCHKLGLNYVSCSPF
CCCCCCCCCCCCCEEEEECCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCC
RVPIARLAAARAALQD
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PNPEKTNEQAAKKQYLYSFAGGASDGDASMKNLLGGKGANLAEMANIGLPVPPGFTIST
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEH
EVCTYYYDNQKNYPPNLFEQDIPDALSKIEYMLGKKFGDPVNPLLVSVRSGARASMPGMM
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCCCHHH
DTILNLGLNSQTVDGLAKRSGNPRFAWDCYRRFVQMYGDVVLDLKPTNKKEIDPFEKILE
HHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHH
EKKHERGITLDTEFTVEDLQDLVSRYKAAILEKTGRTFPEDPHEQLRGAIGAVFGSWNNE
HHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
RAIIYRKLNHIPGYWGTACNVQAMVFGNMGEDCGTGVAFTRDAATGENIFYGEFLMNAQG
CEEEEEEHHCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCCCEEEEHEEECCCC
EDVVAGTRTPLKIEQLKANKPDIYDQLEEIRSSLERHYRDMMDIEFTIESNTLFMLQCRV
CCEEECCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEEEC
GKRTGMAAVKIAVDMFNEGLIDEKEALMRIEPEQLNQLLRPVFNMKEKQAAISGGRLLAT
CCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCEEEEE
GLNAGPGAATGKIYFNAVDAYEASKKGEAVVLVRIETSPEDIKGMAVAEGILTERGGMTS
CCCCCCCCCCCEEEEEEEHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCHH
HAALVARQMGKVCVAGCGALQIDYQKGEMRVAGKDIVLSEGDYISIDGSTGEVIAGKVAT
HHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCEEEEECCCEEEECCCCCCEEEEEEEC
KNSEILEVLIDKTLKPEDASTWPIYKQLMKWADKYRKLKIRTNADQPDQADIAVTFGAEG
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEECCCC
IGLCRTEHMFFGGERIDAMREMILADDITGRQKALEKLLPYQREDFYGLFKCMGERPVTI
CCEEEECCEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCEEE
RLLDPPLHEFLPHTDAEITALAAKIGKSYEEVNMRIEHLHEFNPMLGLRGCRLGILHPEI
EEECCCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHH
AVMQVRAIIEAACQLKKEGLEVIPEIMVPLVSTVKELEITSEIIHKTARSVFAEQGIGVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
YLVGTMIEVPRAAITSDQIATVADFFSYGTNDLTQMGLGMSRDDSGQFLPTYQQQEIFAR
HHHHHHHHCCHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHC
NPFESLDIDGVGRLMSISAKDGRAVKADLKLGICGEHGGDPATVEFCHKLGLNYVSCSPF
CCCCCCCCCCCCCEEEEECCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCC
RVPIARLAAARAALQD
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]