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The map label for this gene is carA [H]

Identifier: 78188090

GI number: 78188090

Start: 121673

End: 122776

Strand: Reverse

Name: carA [H]

Synonym: Cag_0107

Alternate gene names: 78188090

Gene position: 122776-121673 (Counterclockwise)

Preceding gene: 78188091

Following gene: 78188089

Centisome position: 4.77

GC content: 50.63

Gene sequence:

>1104_bases
ATGCAGCCAACACGCGCAAAACTGCTTTTAGAAAATGGCTCACTGTATAGCGGTACGCTTTTTGGGCACATTGGCGAAGC
TATCGGTGAAGTAGTTTTTAACACCTCTCTTACGGGCTATCAGGAAATCCTTACCGATCCCTCTTATGCGGGTCAAATGG
TTACCATGACCTATCCGTTGATTGGCAACTATGGCATTACCCCCAACGATAACGAATCACAGAGCATTTGGGCATCAGCC
TTAATTGTGCATGAGGTTTCGCGCATTCATAGCAATTACGAGGCATCTGGCTCGCTTGCTGCCCGCTTACGTGAAGCACA
AGTTACCGGCTTAGCGGGCATTGATACTCGCCGCCTTGTGCGCGAATTGCGAGAGCATGGTGCCATGCGAGCCATTATTG
CACCTGCTGAAGTTTCGGACGATGAGTTACGCCAAAAAGTTGCGGCTGCTCCCAATATGAGCGGACGCGATTTAGTATCA
ACCGTTACCACGCAAAAAAGCTATTCGTTAGAGACTGAAGGCGCTCAATATCACGTTGTGGCTTTTGATTATGGTATGAA
AACCAACATTTTGCGCTTACTGCAAAATGCGGGCTGCCGTGTTACCGTGCTGCCCGCTCAAGCCACCATGAACGATGTGC
TTGCTCTTAACCCTGATGGAGTCTTCCTCTCCAACGGTCCAGGCGATCCCGCCGCAGTTGGCTATGCAATTGAAACCATT
CGTGCCCTTGTTGAATATAACCGCACCACCAAGCCGCTGCCAATGTTTGGCATCTGCCTTGGGCACCAATTGCTCTCCCT
TGCTTGTGGCGGCACAACCTACAAGTTGAAGTTTGGACACCACGGTGGCAACCACCCCGTGCGCAATCTTGCTACAGGGC
AAATTGAAATTACGTCGCAAAATCACGGTTTTGCGGTAGCAATGGAGGCGCTGCCTGAAGAACTTACCATGACGCACCTC
AATTTGTACGACCACACCGTTGAAGGGGTGCGCCATAAAAACCTCCCCTGCTTTTCGGTGCAATACCACCCCGAAGCGGC
TCCCGGTCCACACGATTCGCACTACCTTTTTGATGAATTCACCTCGCTTATGGCAAGGTCTTAA

Upstream 100 bases:

>100_bases
AAGCGACAACACAAAAATTAAGTTCGACCGCACAGCCATTGCAACTATTGATAAACAGGAAACGGGCGATAAATTGCCCG
GCAAGGAGTAACAGACTATT

Downstream 100 bases:

>100_bases
CGCTCTTTGGTTTTGAGATGAAAAAACGCTTATTCACCCCTGGTCCAACGCCTGTTCCCGAAAATGTGATGCTGCGCATG
GCAGCTCCCATTATTCACCA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MQPTRAKLLLENGSLYSGTLFGHIGEAIGEVVFNTSLTGYQEILTDPSYAGQMVTMTYPLIGNYGITPNDNESQSIWASA
LIVHEVSRIHSNYEASGSLAARLREAQVTGLAGIDTRRLVRELREHGAMRAIIAPAEVSDDELRQKVAAAPNMSGRDLVS
TVTTQKSYSLETEGAQYHVVAFDYGMKTNILRLLQNAGCRVTVLPAQATMNDVLALNPDGVFLSNGPGDPAAVGYAIETI
RALVEYNRTTKPLPMFGICLGHQLLSLACGGTTYKLKFGHHGGNHPVRNLATGQIEITSQNHGFAVAMEALPEELTMTHL
NLYDHTVEGVRHKNLPCFSVQYHPEAAPGPHDSHYLFDEFTSLMARS

Sequences:

>Translated_367_residues
MQPTRAKLLLENGSLYSGTLFGHIGEAIGEVVFNTSLTGYQEILTDPSYAGQMVTMTYPLIGNYGITPNDNESQSIWASA
LIVHEVSRIHSNYEASGSLAARLREAQVTGLAGIDTRRLVRELREHGAMRAIIAPAEVSDDELRQKVAAAPNMSGRDLVS
TVTTQKSYSLETEGAQYHVVAFDYGMKTNILRLLQNAGCRVTVLPAQATMNDVLALNPDGVFLSNGPGDPAAVGYAIETI
RALVEYNRTTKPLPMFGICLGHQLLSLACGGTTYKLKFGHHGGNHPVRNLATGQIEITSQNHGFAVAMEALPEELTMTHL
NLYDHTVEGVRHKNLPCFSVQYHPEAAPGPHDSHYLFDEFTSLMARS
>Mature_367_residues
MQPTRAKLLLENGSLYSGTLFGHIGEAIGEVVFNTSLTGYQEILTDPSYAGQMVTMTYPLIGNYGITPNDNESQSIWASA
LIVHEVSRIHSNYEASGSLAARLREAQVTGLAGIDTRRLVRELREHGAMRAIIAPAEVSDDELRQKVAAAPNMSGRDLVS
TVTTQKSYSLETEGAQYHVVAFDYGMKTNILRLLQNAGCRVTVLPAQATMNDVLALNPDGVFLSNGPGDPAAVGYAIETI
RALVEYNRTTKPLPMFGICLGHQLLSLACGGTTYKLKFGHHGGNHPVRNLATGQIEITSQNHGFAVAMEALPEELTMTHL
NLYDHTVEGVRHKNLPCFSVQYHPEAAPGPHDSHYLFDEFTSLMARS

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=365, Percent_Identity=41.3698630136986, Blast_Score=258, Evalue=6e-69,
Organism=Homo sapiens, GI169790915, Length=376, Percent_Identity=36.968085106383, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI21361331, Length=376, Percent_Identity=36.968085106383, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1786215, Length=378, Percent_Identity=47.8835978835979, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1789760, Length=144, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=368, Percent_Identity=42.3913043478261, Blast_Score=270, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6324878, Length=370, Percent_Identity=40.8108108108108, Blast_Score=252, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6322331, Length=389, Percent_Identity=35.2185089974293, Blast_Score=231, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6322638, Length=179, Percent_Identity=30.7262569832402, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45555749, Length=378, Percent_Identity=38.0952380952381, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24642586, Length=378, Percent_Identity=38.0952380952381, Blast_Score=248, Evalue=6e-66,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39936; Mature: 39936

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPTRAKLLLENGSLYSGTLFGHIGEAIGEVVFNTSLTGYQEILTDPSYAGQMVTMTYPL
CCCCCEEEEEECCCEEECHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEEECE
IGNYGITPNDNESQSIWASALIVHEVSRIHSNYEASGSLAARLREAQVTGLAGIDTRRLV
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
RELREHGAMRAIIAPAEVSDDELRQKVAAAPNMSGRDLVSTVTTQKSYSLETEGAQYHVV
HHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEECCCCEEEEE
AFDYGMKTNILRLLQNAGCRVTVLPAQATMNDVLALNPDGVFLSNGPGDPAAVGYAIETI
EEECCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHH
RALVEYNRTTKPLPMFGICLGHQLLSLACGGTTYKLKFGHHGGNHPVRNLATGQIEITSQ
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHCCCCCEEEEEEC
NHGFAVAMEALPEELTMTHLNLYDHTVEGVRHKNLPCFSVQYHPEAAPGPHDSHYLFDEF
CCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHH
TSLMARS
HHHHHCC
>Mature Secondary Structure
MQPTRAKLLLENGSLYSGTLFGHIGEAIGEVVFNTSLTGYQEILTDPSYAGQMVTMTYPL
CCCCCEEEEEECCCEEECHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEEECE
IGNYGITPNDNESQSIWASALIVHEVSRIHSNYEASGSLAARLREAQVTGLAGIDTRRLV
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
RELREHGAMRAIIAPAEVSDDELRQKVAAAPNMSGRDLVSTVTTQKSYSLETEGAQYHVV
HHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEECCCCEEEEE
AFDYGMKTNILRLLQNAGCRVTVLPAQATMNDVLALNPDGVFLSNGPGDPAAVGYAIETI
EEECCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHH
RALVEYNRTTKPLPMFGICLGHQLLSLACGGTTYKLKFGHHGGNHPVRNLATGQIEITSQ
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHCCCCCEEEEEEC
NHGFAVAMEALPEELTMTHLNLYDHTVEGVRHKNLPCFSVQYHPEAAPGPHDSHYLFDEF
CCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHH
TSLMARS
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA