Click here to switch to the map view.

The map label for this gene is gyrB [H]

Identifier: 78188013

GI number: 78188013

Start: 31358

End: 33271

Strand: Reverse

Name: gyrB [H]

Synonym: Cag_0029

Alternate gene names: 78188013

Gene position: 33271-31358 (Counterclockwise)

Preceding gene: 78188015

Following gene: 78188009

Centisome position: 1.29

GC content: 46.87

Gene sequence:

>1914_bases
GTGCCACCAGCGGCATATGGCGCTACCAACATTCAAGTGCTTGATGGTATTGAGCATGTGCGTATGCGTCCGGCAATGTA
CATTGGCGATATTCATAGCCGTGGTTTGCACCATTTGGTGTATGAAATTGTTGATAACTCCATTGACGAAACGCTTGGTG
GCTTTAACGACTACATTTTTGTAGCACTCAACGCCGATGGCTCCATTACCGTTATTGACCACGGTCGAGGCATTCCGGTG
GATATGCACCCTGAAAAGCAGAAGTCAGCGCTTGAGTTAGTTATGACGGTTATTGGCGCTGGTGGTAAGTTCGATAAAGG
TGCTTACAAGGTGTCGGGAGGTTTACACGGCGTTGGTGCATCGGTAGTAAATGCGCTTTCGGAATGGTGCGAAGTTGAGG
TCTATCGCGATGGCAAGGCGTACTACCAGCGTTACGAACGTGGCGTGCCACAAGGCGATGTTAAGGTAATTGGCGACTCC
GATCAGCGAGGTACCAAAACCACCTTTATGCCTGATGGCACCATTTTTAAAACCACCGAGTTCCGTAAAGAGATTATTAT
TGATAGAATGCGAGAGTTGGCATTCCTCAATAAAAACCTACGCATTATTGTTCAAGATACGAATGGCGAACAAGAGGAAT
TCCATTTTGAAGGTGGCATTTGTGAATTTGTACGCTTTACCGATCAAAACCGCCTGAACCTTCTGCGAGAACCCATTTAT
CTCTACGGTGAGCGCGATGGTACCGTGGTTGAAATTGCACTGCAATACAACGACTCCTATCAAGAAAACGTCTTTAGCTA
CGTTAACAACATTAACACGCATGAAGGCGGCACGCACGTTACAGGTTTCCGTAAAGCTTTAACGCGCACCCTCAATTCCT
ACGCGCAAAAAAATGATTTGCTGAAAAACTTGAAGTTAACCCTTACGGGCGATGACTTTAAGGAGGGGCTAACAGCGGTG
ATTTCGGTAAAGGTTGCCGAACCGCAGTTTGAGGGGCAAACCAAAACCAAGCTTGGCAACTCCGAAACGCAAAGCATTGT
AGAAACCGTTGTTAATGATCAACTTGCAGAGTTTGCCGAAAGCAACCCCAACACGCTCAAGCTGATTATTGAGAAGGTGA
AAGGTGCTGCCATGTCGCGCGAAGCTGCCCGCAAAGCAAAGGAACTTACACGCCGCAAATCGGTGCTCGAAAGTTCAGGC
TTGCCCGGCAAGCTTGCTGATTGCTCCATTAACGACCCTGAGCATTGCGAACTTTACATTGTGGAGGGCGATTCGGCAGG
TGGTAGTGCCAAGCAAGGAAGAGATCGCAGCTTTCAAGCCATTTTACCGCTTAAGGGTAAAATTCTCAACGTTGAAAAAG
CACGGCTGCATAAAATGCTGGAAAACGAGGAAATTAAAACAATCATTCTTGCGCTTGGCACCAGCTTTGGCGATGAAGAG
TTTGCGGTAGAAAAATTACGCTACGGCAAAATTATTATTATGACTGATGCCGACGTTGATGGTGCCCACATTCGCACGTT
GTTGCTAACCTTTTTCTTTCGCCACATGCGTCCCGTGATTGAGGCTGGAAGAGTTTATATTGCCCAGCCACCACTTTATT
TGGTGAAATCGGGTAAAGATCAGCACTATGCGTGGGATGATGATGAGCGCAACAGCATTGTGGATAACATGAAGAAAATG
CAGAAGAGTAAAGCCAACATCCACATTCAGCGCTACAAAGGTCTTGGAGAAATGAACCCCGAACAGCTTTGGAGCACCAC
CATGGATCCTGCCCACCGCTCACTTTTACTTGTAAGTGTAGAAAATGCTATGGAAGCCGATCAGGTGTTTTCAACGCTTA
TGGGCGATAAGGTTGAACCACGCCGCGAATTTATTGAGAAAAATGCACGCTACGTTCGCCGCTTAGATGTTTAA

Upstream 100 bases:

>100_bases
CGAGACCTCTCTTTTTGATGCAACCTCTGATGCTCCAACGCCCACTGAAGTAGTTCCTGACGTTCCAGCTCTTGCCGAAA
CGCCTACGCCTTTAGCCGAT

Downstream 100 bases:

>100_bases
GCGCTGAACATTTCCGCCCCATTTTCTTTAGAGAGCAACTCAATCCAATGGGAGATGAGTTGCTCTTTTTTTATTGACCT
TTTTATTCGTCGTCAATGAG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MPPAAYGATNIQVLDGIEHVRMRPAMYIGDIHSRGLHHLVYEIVDNSIDETLGGFNDYIFVALNADGSITVIDHGRGIPV
DMHPEKQKSALELVMTVIGAGGKFDKGAYKVSGGLHGVGASVVNALSEWCEVEVYRDGKAYYQRYERGVPQGDVKVIGDS
DQRGTKTTFMPDGTIFKTTEFRKEIIIDRMRELAFLNKNLRIIVQDTNGEQEEFHFEGGICEFVRFTDQNRLNLLREPIY
LYGERDGTVVEIALQYNDSYQENVFSYVNNINTHEGGTHVTGFRKALTRTLNSYAQKNDLLKNLKLTLTGDDFKEGLTAV
ISVKVAEPQFEGQTKTKLGNSETQSIVETVVNDQLAEFAESNPNTLKLIIEKVKGAAMSREAARKAKELTRRKSVLESSG
LPGKLADCSINDPEHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALGTSFGDEE
FAVEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRHMRPVIEAGRVYIAQPPLYLVKSGKDQHYAWDDDERNSIVDNMKKM
QKSKANIHIQRYKGLGEMNPEQLWSTTMDPAHRSLLLVSVENAMEADQVFSTLMGDKVEPRREFIEKNARYVRRLDV

Sequences:

>Translated_637_residues
MPPAAYGATNIQVLDGIEHVRMRPAMYIGDIHSRGLHHLVYEIVDNSIDETLGGFNDYIFVALNADGSITVIDHGRGIPV
DMHPEKQKSALELVMTVIGAGGKFDKGAYKVSGGLHGVGASVVNALSEWCEVEVYRDGKAYYQRYERGVPQGDVKVIGDS
DQRGTKTTFMPDGTIFKTTEFRKEIIIDRMRELAFLNKNLRIIVQDTNGEQEEFHFEGGICEFVRFTDQNRLNLLREPIY
LYGERDGTVVEIALQYNDSYQENVFSYVNNINTHEGGTHVTGFRKALTRTLNSYAQKNDLLKNLKLTLTGDDFKEGLTAV
ISVKVAEPQFEGQTKTKLGNSETQSIVETVVNDQLAEFAESNPNTLKLIIEKVKGAAMSREAARKAKELTRRKSVLESSG
LPGKLADCSINDPEHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALGTSFGDEE
FAVEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRHMRPVIEAGRVYIAQPPLYLVKSGKDQHYAWDDDERNSIVDNMKKM
QKSKANIHIQRYKGLGEMNPEQLWSTTMDPAHRSLLLVSVENAMEADQVFSTLMGDKVEPRREFIEKNARYVRRLDV
>Mature_636_residues
PPAAYGATNIQVLDGIEHVRMRPAMYIGDIHSRGLHHLVYEIVDNSIDETLGGFNDYIFVALNADGSITVIDHGRGIPVD
MHPEKQKSALELVMTVIGAGGKFDKGAYKVSGGLHGVGASVVNALSEWCEVEVYRDGKAYYQRYERGVPQGDVKVIGDSD
QRGTKTTFMPDGTIFKTTEFRKEIIIDRMRELAFLNKNLRIIVQDTNGEQEEFHFEGGICEFVRFTDQNRLNLLREPIYL
YGERDGTVVEIALQYNDSYQENVFSYVNNINTHEGGTHVTGFRKALTRTLNSYAQKNDLLKNLKLTLTGDDFKEGLTAVI
SVKVAEPQFEGQTKTKLGNSETQSIVETVVNDQLAEFAESNPNTLKLIIEKVKGAAMSREAARKAKELTRRKSVLESSGL
PGKLADCSINDPEHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALGTSFGDEEF
AVEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRHMRPVIEAGRVYIAQPPLYLVKSGKDQHYAWDDDERNSIVDNMKKMQ
KSKANIHIQRYKGLGEMNPEQLWSTTMDPAHRSLLLVSVENAMEADQVFSTLMGDKVEPRREFIEKNARYVRRLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=603, Percent_Identity=25.2072968490879, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI19913406, Length=615, Percent_Identity=25.5284552845528, Blast_Score=126, Evalue=5e-29,
Organism=Escherichia coli, GI48994957, Length=547, Percent_Identity=54.4789762340037, Blast_Score=580, Evalue=1e-166,
Organism=Escherichia coli, GI1789408, Length=629, Percent_Identity=38.9507154213037, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17535065, Length=664, Percent_Identity=24.6987951807229, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI212645845, Length=597, Percent_Identity=24.9581239530988, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI212645657, Length=187, Percent_Identity=28.3422459893048, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=565, Percent_Identity=25.3097345132743, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17136538, Length=671, Percent_Identity=23.8450074515648, Blast_Score=127, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71593; Mature: 71462

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPAAYGATNIQVLDGIEHVRMRPAMYIGDIHSRGLHHLVYEIVDNSIDETLGGFNDYIF
CCCCCCCCCCEEEHHCHHHHHHCCHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VALNADGSITVIDHGRGIPVDMHPEKQKSALELVMTVIGAGGKFDKGAYKVSGGLHGVGA
EEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHH
SVVNALSEWCEVEVYRDGKAYYQRYERGVPQGDVKVIGDSDQRGTKTTFMPDGTIFKTTE
HHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCEEEECCCCCCCCEEEECCCCCEEECHH
FRKEIIIDRMRELAFLNKNLRIIVQDTNGEQEEFHFEGGICEFVRFTDQNRLNLLREPIY
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEECCCCEEEHHHCCCHHHHHHHCCEE
LYGERDGTVVEIALQYNDSYQENVFSYVNNINTHEGGTHVTGFRKALTRTLNSYAQKNDL
EEECCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LKNLKLTLTGDDFKEGLTAVISVKVAEPQFEGQTKTKLGNSETQSIVETVVNDQLAEFAE
HHCCEEEEECCHHHHCCEEEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
SNPNTLKLIIEKVKGAAMSREAARKAKELTRRKSVLESSGLPGKLADCSINDPEHCELYI
CCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEE
VEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALGTSFGDEE
EECCCCCCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHCCCCHHEEEEECCCCCCCHH
FAVEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRHMRPVIEAGRVYIAQPPLYLVKSGKD
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCC
QHYAWDDDERNSIVDNMKKMQKSKANIHIQRYKGLGEMNPEQLWSTTMDPAHRSLLLVSV
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCCCCHHHEEEEEEE
ENAMEADQVFSTLMGDKVEPRREFIEKNARYVRRLDV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PPAAYGATNIQVLDGIEHVRMRPAMYIGDIHSRGLHHLVYEIVDNSIDETLGGFNDYIF
CCCCCCCCCEEEHHCHHHHHHCCHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VALNADGSITVIDHGRGIPVDMHPEKQKSALELVMTVIGAGGKFDKGAYKVSGGLHGVGA
EEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHH
SVVNALSEWCEVEVYRDGKAYYQRYERGVPQGDVKVIGDSDQRGTKTTFMPDGTIFKTTE
HHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCEEEECCCCCCCCEEEECCCCCEEECHH
FRKEIIIDRMRELAFLNKNLRIIVQDTNGEQEEFHFEGGICEFVRFTDQNRLNLLREPIY
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEECCCCEEEHHHCCCHHHHHHHCCEE
LYGERDGTVVEIALQYNDSYQENVFSYVNNINTHEGGTHVTGFRKALTRTLNSYAQKNDL
EEECCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LKNLKLTLTGDDFKEGLTAVISVKVAEPQFEGQTKTKLGNSETQSIVETVVNDQLAEFAE
HHCCEEEEECCHHHHCCEEEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
SNPNTLKLIIEKVKGAAMSREAARKAKELTRRKSVLESSGLPGKLADCSINDPEHCELYI
CCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEE
VEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALGTSFGDEE
EECCCCCCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHCCCCHHEEEEECCCCCCCHH
FAVEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRHMRPVIEAGRVYIAQPPLYLVKSGKD
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCC
QHYAWDDDERNSIVDNMKKMQKSKANIHIQRYKGLGEMNPEQLWSTTMDPAHRSLLLVSV
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCCCCHHHEEEEEEE
ENAMEADQVFSTLMGDKVEPRREFIEKNARYVRRLDV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10427704; 11058132 [H]