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The map label for this gene is mscL

Identifier: 78188009

GI number: 78188009

Start: 25717

End: 26184

Strand: Reverse

Name: mscL

Synonym: Cag_0025

Alternate gene names: 78188009

Gene position: 26184-25717 (Counterclockwise)

Preceding gene: 78188013

Following gene: 78188006

Centisome position: 1.02

GC content: 43.59

Gene sequence:

>468_bases
ATGTCGGTTATGAAAGAGTTTAAAGAATTTGCGGTTAAGGGCAATGTGGTTGACATGGCAGTTGGTATTATTATTGGTGG
TGCCTTTGGCGCTATTGTCAATAACTTGGTATCGCAAGTTATTCTGCCTCCATTAGGTTTACTTATTGGCGGAGTTGATT
TTAGTAGCCTTTATATAATTTTAAAAGAGGGGGCAACACAAGCCGCACCTTATAATTCGCTTGCTGAAGCAACAGCCGCA
GGAGCCGTAACATTGAATTATGGCGTATTTTTGAATTCGGTGTTTAGCTTTGTGATTATGGCGTTTGCCGTCTTTTTGTT
GGTTAAGAGCATTAACATGCTGCGCCGCACGGAGGGAAGCAAAAGTGCCGTACCAGCGCCATCTACCACAAAAGAGTGCC
CTTATTGCTTGAGCAGTGTACCACTAAAAGCAACTCGCTGCCCTCAGTGTACGTCAGAATTGAAGTAG

Upstream 100 bases:

>100_bases
AAAGAGCCTTTTTTTATACATGGGGATTGCAAGTACTGCCCCGAGCTGCTATGGAGATACAGAATAACATCCCATGCAAT
CAAACAAGGAGAAGTGTAAC

Downstream 100 bases:

>100_bases
TATCGTATCAAGTTCTTTCTGTCATGCTGAGCGAAGCGAAGCATCCATACAATTTAGCGGCTTTTGGCTGGATTTTAAGA
TGGATTCTTCACTTCGTTCC

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MSVMKEFKEFAVKGNVVDMAVGIIIGGAFGAIVNNLVSQVILPPLGLLIGGVDFSSLYIILKEGATQAAPYNSLAEATAA
GAVTLNYGVFLNSVFSFVIMAFAVFLLVKSINMLRRTEGSKSAVPAPSTTKECPYCLSSVPLKATRCPQCTSELK

Sequences:

>Translated_155_residues
MSVMKEFKEFAVKGNVVDMAVGIIIGGAFGAIVNNLVSQVILPPLGLLIGGVDFSSLYIILKEGATQAAPYNSLAEATAA
GAVTLNYGVFLNSVFSFVIMAFAVFLLVKSINMLRRTEGSKSAVPAPSTTKECPYCLSSVPLKATRCPQCTSELK
>Mature_154_residues
SVMKEFKEFAVKGNVVDMAVGIIIGGAFGAIVNNLVSQVILPPLGLLIGGVDFSSLYIILKEGATQAAPYNSLAEATAAG
AVTLNYGVFLNSVFSFVIMAFAVFLLVKSINMLRRTEGSKSAVPAPSTTKECPYCLSSVPLKATRCPQCTSELK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=132, Percent_Identity=50.7575757575758, Blast_Score=130, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_CHLCH (Q3ANR9)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_378347.1
- ProteinModelPortal:   Q3ANR9
- SMR:   Q3ANR9
- STRING:   Q3ANR9
- GeneID:   3746994
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_0025
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- ProtClustDB:   PRK00567
- BioCyc:   CCHL340177:CAG_0025-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 16328; Mature: 16197

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x44887d50)-; HASH(0x44975320)-; HASH(0x449c8ae0)-;

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVMKEFKEFAVKGNVVDMAVGIIIGGAFGAIVNNLVSQVILPPLGLLIGGVDFSSLYII
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LKEGATQAAPYNSLAEATAAGAVTLNYGVFLNSVFSFVIMAFAVFLLVKSINMLRRTEGS
EECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KSAVPAPSTTKECPYCLSSVPLKATRCPQCTSELK
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHCCH
>Mature Secondary Structure 
SVMKEFKEFAVKGNVVDMAVGIIIGGAFGAIVNNLVSQVILPPLGLLIGGVDFSSLYII
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LKEGATQAAPYNSLAEATAAGAVTLNYGVFLNSVFSFVIMAFAVFLLVKSINMLRRTEGS
EECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KSAVPAPSTTKECPYCLSSVPLKATRCPQCTSELK
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA