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The map label for this gene is ada [H]

Identifier: 78067977

GI number: 78067977

Start: 3680616

End: 3681707

Strand: Reverse

Name: ada [H]

Synonym: Bcep18194_A6508

Alternate gene names: 78067977

Gene position: 3681707-3680616 (Counterclockwise)

Preceding gene: 78067979

Following gene: 78067976

Centisome position: 99.66

GC content: 69.69

Gene sequence:

>1092_bases
ATGAAAACCGCCTATCCGACCGATGATGCCCGCTGGGGCGCCGTCACCGAGCGCGATCCGCATGCGGACGGCGCGTTCTT
CTATGCCGTGAGCACGACCGGCGTGTTCTGCCGCCCGACGTGCGCGTCGCGGCAGCCGCGTCGCGAAAACGTGTCCTTCT
TCGTCGATACGGCCGCCGCGCGTGCGGCCGGCTTCCGGCCCTGCAAGCGCTGCCAGCCGGAAGGGCTGCCGCGCGAGCTC
GAGATCGTCAACCGCGCGTGCGCGGTGCTCGACGCACATGCCGAACGGCTCACGCTGCAACAACTGAGCGATGCCGTGCA
CGTGAGCCCGTTTCACCTTCAGCGGCTCTTCAAGCGCGTGGTCGGCGTGTCGCCGCGGCAGTACCAGGCCGCACAGCGCG
GCGCCGCGCTGCGGCAGGCGCTGCAAAGCGGGCAGCCGGTCACGCAGGCGGCCGTCGACGCCGGCTTCAACTCGCCGTCG
CGGCTCTATGCGTCGGTGCCGCGCGAACTCGGGATGGCGCCGTCGGCGTTCCGCCGCCAGGGTGCGGGTCTCAGGATCGA
CTATGCGACCGCATCGACTTCGCTCGGCACGGTGCTCGTCGCCGCGACCGAACAGGGCATCTGCCGGATCGCGTTCGGCG
ACGAACCGGCCGCGCTCGTCGGCGAGCTGAAGGATGCGTTCGCACGTGCCGAGCTGGTCGAATCACAGGCACGACTCGTG
CCGTTCGTCTCGCAGATTCGCGCGTACCTGGACGGCACGCGGCACGCATTCGACCTGCCGCTCGACATCGCGCCGACTGC
ATTCCAGCAGCGCGTATGGGAGGCGCTGACGCATATTCCGTACGGCGAAACGCGCAGCTACTCGGAGATCGCCGAGGCGC
TGGGTTCGCCGCGCGCGGTGCGTGCCGTCGCGTCCGCGTGCGCGTCGAACCCGGTCGCGCTCGCGATTCCGTGCCACCGC
GTCGTACAGAAGGGCGGCGCGCTTTCCGGGTATCGTTGGGGCGTGCGCCGCAAGGCGACGCTGCTCGCGTCCGAGGCGCG
GCATGTGAACGACGATCAGACCGAATCCGTTGCGGAGGGCAGTGCAGTTTGA

Upstream 100 bases:

>100_bases
GGCGAGTCAGCGCAAGAATCACAGAGCAAGATTCAGGGCGCGACGGCACGTGCCGATGTCTAGAGTAGGCACCATCTACA
CTAGAGAGTGCGAGGTTCAG

Downstream 100 bases:

>100_bases
GCATCGCAAAAACAACGATTACGTCCATCGTGAACGGCGGCCAGGTGCCGCCGTCCGCGCAAATGGAGCTGCGCTTCAAT
GCGCCTTACGACTGGGCGCG

Product: bifunctional DNA-O6-methylguanine--protein-cysteine S-methyltransferase / transcriptional regulator Ada

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKTAYPTDDARWGAVTERDPHADGAFFYAVSTTGVFCRPTCASRQPRRENVSFFVDTAAARAAGFRPCKRCQPEGLPREL
EIVNRACAVLDAHAERLTLQQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRQALQSGQPVTQAAVDAGFNSPS
RLYASVPRELGMAPSAFRRQGAGLRIDYATASTSLGTVLVAATEQGICRIAFGDEPAALVGELKDAFARAELVESQARLV
PFVSQIRAYLDGTRHAFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAEALGSPRAVRAVASACASNPVALAIPCHR
VVQKGGALSGYRWGVRRKATLLASEARHVNDDQTESVAEGSAV

Sequences:

>Translated_363_residues
MKTAYPTDDARWGAVTERDPHADGAFFYAVSTTGVFCRPTCASRQPRRENVSFFVDTAAARAAGFRPCKRCQPEGLPREL
EIVNRACAVLDAHAERLTLQQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRQALQSGQPVTQAAVDAGFNSPS
RLYASVPRELGMAPSAFRRQGAGLRIDYATASTSLGTVLVAATEQGICRIAFGDEPAALVGELKDAFARAELVESQARLV
PFVSQIRAYLDGTRHAFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAEALGSPRAVRAVASACASNPVALAIPCHR
VVQKGGALSGYRWGVRRKATLLASEARHVNDDQTESVAEGSAV
>Mature_363_residues
MKTAYPTDDARWGAVTERDPHADGAFFYAVSTTGVFCRPTCASRQPRRENVSFFVDTAAARAAGFRPCKRCQPEGLPREL
EIVNRACAVLDAHAERLTLQQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRQALQSGQPVTQAAVDAGFNSPS
RLYASVPRELGMAPSAFRRQGAGLRIDYATASTSLGTVLVAATEQGICRIAFGDEPAALVGELKDAFARAELVESQARLV
PFVSQIRAYLDGTRHAFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAEALGSPRAVRAVASACASNPVALAIPCHR
VVQKGGALSGYRWGVRRKATLLASEARHVNDDQTESVAEGSAV

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=174, Percent_Identity=29.8850574712644, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1788542, Length=345, Percent_Identity=48.9855072463768, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI1787596, Length=166, Percent_Identity=36.144578313253, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI115533070, Length=125, Percent_Identity=44, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=125, Percent_Identity=44, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320001, Length=156, Percent_Identity=28.8461538461538, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI17137554, Length=87, Percent_Identity=55.1724137931034, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 39202; Mature: 39202

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAYPTDDARWGAVTERDPHADGAFFYAVSTTGVFCRPTCASRQPRRENVSFFVDTAAA
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCCEEEEEHHHH
RAAGFRPCKRCQPEGLPRELEIVNRACAVLDAHAERLTLQQLSDAVHVSPFHLQRLFKRV
HHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VGVSPRQYQAAQRGAALRQALQSGQPVTQAAVDAGFNSPSRLYASVPRELGMAPSAFRRQ
HCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHC
GAGLRIDYATASTSLGTVLVAATEQGICRIAFGDEPAALVGELKDAFARAELVESQARLV
CCCEEEEECCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
PFVSQIRAYLDGTRHAFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAEALGSPRAV
HHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
RAVASACASNPVALAIPCHRVVQKGGALSGYRWGVRRKATLLASEARHVNDDQTESVAEG
HHHHHHHCCCCEEEEECHHHHHHCCCCCCCHHHCHHHHHHHHHHHHHCCCCCHHHHHHCC
SAV
CCC
>Mature Secondary Structure
MKTAYPTDDARWGAVTERDPHADGAFFYAVSTTGVFCRPTCASRQPRRENVSFFVDTAAA
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCCEEEEEHHHH
RAAGFRPCKRCQPEGLPRELEIVNRACAVLDAHAERLTLQQLSDAVHVSPFHLQRLFKRV
HHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VGVSPRQYQAAQRGAALRQALQSGQPVTQAAVDAGFNSPSRLYASVPRELGMAPSAFRRQ
HCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHC
GAGLRIDYATASTSLGTVLVAATEQGICRIAFGDEPAALVGELKDAFARAELVESQARLV
CCCEEEEECCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
PFVSQIRAYLDGTRHAFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAEALGSPRAV
HHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
RAVASACASNPVALAIPCHRVVQKGGALSGYRWGVRRKATLLASEARHVNDDQTESVAEG
HHHHHHHCCCCEEEEECHHHHHHCCCCCCCHHHCHHHHHHHHHHHHHCCCCCHHHHHHCC
SAV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]