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The map label for this gene is gshA [H]

Identifier: 78067974

GI number: 78067974

Start: 3677471

End: 3679084

Strand: Reverse

Name: gshA [H]

Synonym: Bcep18194_A6505

Alternate gene names: 78067974

Gene position: 3679084-3677471 (Counterclockwise)

Preceding gene: 78067976

Following gene: 78067972

Centisome position: 99.59

GC content: 66.36

Gene sequence:

>1614_bases
ATGTCGAACACCATGACTCACCGCCAGTCCGAACTGCTGCTGAATCGCCTCGAAGCGCTGAGCTCGGGCCCGACGCGGCA
GCATCTGCCCGACGGCCTGCGCGGCATCGAAAAGGAAAGCCTGCGCGTGACGCGCGACGGGATGATCGCGTTCACGCCGC
ATCCGCGCGCGCTCGGTTCGGCGCTCACGCATCCGTCGTTGACGACCGACTATTCCGAAGCGCTGATCGAGCTGATCACG
CCCGCGGAAGGCGATGCTGCGATCACGCTCGAACGTCTCGACGATCTGCATCGCTACGTGTATGCGTCGCTCGGCGACGA
GATGCTGTGGAACGACTCGATGCCCGGCCTGCTGCCGGCCGACGACCAGATTCCGATTGCCGACTACGGCACGTCGAACA
TCGGCCGCCTGAAGACGGTGTACCGGCGCGGCCTCGCGTATCGCTACGGCCGCACGATGCAGTGCATTGCCGGCATCCAC
TACAACTACTCGCTGCACGAGGAAGTGTGGCGGCGCCTGCATGCGGAAGAAGGCTCGACGGCCACGCTCGTCGATTACCA
GTCGGAGCGCTATCTCGCGCAGATCCGCAACTTCCGCCGCCGCAGCTGGCTGCTGATGTACCTGTTCGGCGCGTCGCCCG
CGCTCGACACGAAGTTCCTGCGCGGCAAGCCGCACAAGCTCGACACGTTCGACGCCGATACGCTGTACTTGCCGTATGCG
ACGAGCCTGCGGATGAGCGATCTCGGCTACTCGAACACGACCGCCCAGGCCGCGCTGCACGTCGACTACAACACGCTGCC
CGGCTATCTCGACGCGCTGTCGACGGCCGTGAGCGAGCCGTATCCGGCCTACGAGGCGATCGGCACGCATCGCGACGGCG
AGTGGATCCAGATCAACACCAACGTGCTGCAGATCGAGAACGAGTTCTACTCGACGATCCGGCCGAAGCGCGTCACGTAC
TCGGGCGAGCGGCCGCTGCATGCGCTCGCGTCGCGCGGCGTGCAGTACATCGAGGTGCGCTGTCTCGACATCGATCCGTT
CGAGCCGACCGGCATCGCGCTGCAAACCGCGCGCTTCATCGACGCGTTCCTGCTCGCGTGCGCGCTCGACGAAAGCCCGG
CGCTCGATTGCCCTGCGTACAAGGAAGCGAACGCAAACTTCGGCAGCGTGACGATGGAAGGGCGCAAGCCGGGTCTCACG
CTGCAGCGCGACGGCCAGCCGGTCACGCTGCAGGCGTGGGCGGACGACCTGATGGCCGATATCGAAACCGTCGGCCGTCG
TCTCGACGAAATCCGCGGCGGCGACGAGCACGTGCGCGCGATTGCCGCGCAACGCGAGAAACTGGCCGATCCGGAGCGCA
CGCCGTCCGCGCACGTGCTGCGCACGATGCGCGAACGCGGTCAGTCGTTCCTCGGGTTCGCGCTGGCGCAGAGCGAAGCG
CATGCCGCGCATTTCCGCGCGCATCCGCCGTCGGCGGATACGCTGCGCACCGAGCAGGCGCTTGCCGCGAAATCGCTGGC
CGAGCAGGCCGAGCTCGAAGCGAAGGAGGCCGGATCGTTCGACGCGTTCGTCGCGGCCTATCGCGCCTATACGTTGAACC
GCTTCAGCGTGTAA

Upstream 100 bases:

>100_bases
TTCGGCGAGATGTTAAAGTGCCCGCTACCAACGAAGCGACCAACAATCCAGCCGGCCGCGCGCGGCGTTCTGCGCGCGGC
CGTCACGCACTTGCGTCTCC

Downstream 100 bases:

>100_bases
TGCCTGGCGCACGTGCACCGCGTCGGTCGGCGCGGTGCACGCTGCGTATCTCCGTCGCCGGTCAGGGCGCTCCGTTGTGC
GCTCGATCCGGTTCGATCAG

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSNTMTHRQSELLLNRLEALSSGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTTDYSEALIELIT
PAEGDAAITLERLDDLHRYVYASLGDEMLWNDSMPGLLPADDQIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIH
YNYSLHEEVWRRLHAEEGSTATLVDYQSERYLAQIRNFRRRSWLLMYLFGASPALDTKFLRGKPHKLDTFDADTLYLPYA
TSLRMSDLGYSNTTAQAALHVDYNTLPGYLDALSTAVSEPYPAYEAIGTHRDGEWIQINTNVLQIENEFYSTIRPKRVTY
SGERPLHALASRGVQYIEVRCLDIDPFEPTGIALQTARFIDAFLLACALDESPALDCPAYKEANANFGSVTMEGRKPGLT
LQRDGQPVTLQAWADDLMADIETVGRRLDEIRGGDEHVRAIAAQREKLADPERTPSAHVLRTMRERGQSFLGFALAQSEA
HAAHFRAHPPSADTLRTEQALAAKSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV

Sequences:

>Translated_537_residues
MSNTMTHRQSELLLNRLEALSSGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTTDYSEALIELIT
PAEGDAAITLERLDDLHRYVYASLGDEMLWNDSMPGLLPADDQIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIH
YNYSLHEEVWRRLHAEEGSTATLVDYQSERYLAQIRNFRRRSWLLMYLFGASPALDTKFLRGKPHKLDTFDADTLYLPYA
TSLRMSDLGYSNTTAQAALHVDYNTLPGYLDALSTAVSEPYPAYEAIGTHRDGEWIQINTNVLQIENEFYSTIRPKRVTY
SGERPLHALASRGVQYIEVRCLDIDPFEPTGIALQTARFIDAFLLACALDESPALDCPAYKEANANFGSVTMEGRKPGLT
LQRDGQPVTLQAWADDLMADIETVGRRLDEIRGGDEHVRAIAAQREKLADPERTPSAHVLRTMRERGQSFLGFALAQSEA
HAAHFRAHPPSADTLRTEQALAAKSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV
>Mature_536_residues
SNTMTHRQSELLLNRLEALSSGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTTDYSEALIELITP
AEGDAAITLERLDDLHRYVYASLGDEMLWNDSMPGLLPADDQIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHY
NYSLHEEVWRRLHAEEGSTATLVDYQSERYLAQIRNFRRRSWLLMYLFGASPALDTKFLRGKPHKLDTFDADTLYLPYAT
SLRMSDLGYSNTTAQAALHVDYNTLPGYLDALSTAVSEPYPAYEAIGTHRDGEWIQINTNVLQIENEFYSTIRPKRVTYS
GERPLHALASRGVQYIEVRCLDIDPFEPTGIALQTARFIDAFLLACALDESPALDCPAYKEANANFGSVTMEGRKPGLTL
QRDGQPVTLQAWADDLMADIETVGRRLDEIRGGDEHVRAIAAQREKLADPERTPSAHVLRTMRERGQSFLGFALAQSEAH
AAHFRAHPPSADTLRTEQALAAKSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=504, Percent_Identity=43.6507936507937, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 60017; Mature: 59886

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTMTHRQSELLLNRLEALSSGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGS
CCCCCHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCHHHEEEEECCEEEECCCCHHHHH
ALTHPSLTTDYSEALIELITPAEGDAAITLERLDDLHRYVYASLGDEMLWNDSMPGLLPA
HHCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCC
DDQIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSLHEEVWRRLHAEEGST
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHEEHHCCCCCCCHHHHHHHHHHCCCCCE
ATLVDYQSERYLAQIRNFRRRSWLLMYLFGASPALDTKFLRGKPHKLDTFDADTLYLPYA
EEEEECCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCEEEECCC
TSLRMSDLGYSNTTAQAALHVDYNTLPGYLDALSTAVSEPYPAYEAIGTHRDGEWIQINT
CCEEHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEC
NVLQIENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALQTARFI
CEEEECHHHHHHCCCCEEEECCCCCHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHH
DAFLLACALDESPALDCPAYKEANANFGSVTMEGRKPGLTLQRDGQPVTLQAWADDLMAD
HHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCEEEHHHHHHHHHH
IETVGRRLDEIRGGDEHVRAIAAQREKLADPERTPSAHVLRTMRERGQSFLGFALAQSEA
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCH
HAAHFRAHPPSADTLRTEQALAAKSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNTMTHRQSELLLNRLEALSSGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGS
CCCCHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCHHHEEEEECCEEEECCCCHHHHH
ALTHPSLTTDYSEALIELITPAEGDAAITLERLDDLHRYVYASLGDEMLWNDSMPGLLPA
HHCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCC
DDQIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSLHEEVWRRLHAEEGST
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHEEHHCCCCCCCHHHHHHHHHHCCCCCE
ATLVDYQSERYLAQIRNFRRRSWLLMYLFGASPALDTKFLRGKPHKLDTFDADTLYLPYA
EEEEECCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCEEEECCC
TSLRMSDLGYSNTTAQAALHVDYNTLPGYLDALSTAVSEPYPAYEAIGTHRDGEWIQINT
CCEEHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEC
NVLQIENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALQTARFI
CEEEECHHHHHHCCCCEEEECCCCCHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHH
DAFLLACALDESPALDCPAYKEANANFGSVTMEGRKPGLTLQRDGQPVTLQAWADDLMAD
HHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCEEEHHHHHHHHHH
IETVGRRLDEIRGGDEHVRAIAAQREKLADPERTPSAHVLRTMRERGQSFLGFALAQSEA
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCH
HAAHFRAHPPSADTLRTEQALAAKSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA