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The map label for this gene is dhaAF [H]

Identifier: 78067957

GI number: 78067957

Start: 3657670

End: 3658485

Strand: Direct

Name: dhaAF [H]

Synonym: Bcep18194_A6488

Alternate gene names: 78067957

Gene position: 3657670-3658485 (Clockwise)

Preceding gene: 78067955

Following gene: 78067963

Centisome position: 99.01

GC content: 68.26

Gene sequence:

>816_bases
ATGCTCGAACTCGCCAATCGCTTCATTTTCGAAGGCCACCGGATTGCCTGGGGAACGCTCGGCGAAGGCCCGCCGCTCGT
GCTCGTGCACGGCACGCCGTTTTCGTCGCAGGTGTGGCGGCGGATCGCACCGTGGCTCGCGCGGCGTCATCGCGTGTTCT
TCTACGACCTGCTCGGCTACGGTCAGTCCGACATGCCTGACGCGGACGTGTCGCTCAGCCGCCAGAACCGGCTGTTCGGC
GCGATGCTCGACGCGTGGAACATTTCGCGTCCGCGCGTGCTCGCGCACGACTACGGCGGCGCAACCGTGCTGCGCGCGCA
CTTCCTCGACGGCGTCGACTATGCGGATCTCACCCTCGTGAATCCGGTCGCGATTACGCCGCAGGGTTCGCCGTTCGTGC
GCCACGTCGCGCAGCACGAAGCCGCCTTCACCGGATTGCCTGCGTATGCGCATCACGCGCTCGTGTCGGCCTATCTCGAC
AATGCGGTCGCCCGGCCGCTGAGCGACGAGGCGCTGTCGATCTACCGTGCGCCGTGGCTCACGCCCGACGGTCAGGCCGC
GTTCTATCGCCAGATCGCGCAGATGCGCCAGCGCTACATCGAGGAAGTCGAGGCGCGTTACGCGCCGCCGGACTTTCCGG
TGCGCATCGTGTGGGGCGAGGACGACGCGTGGATTCCGCTCGAACAGGGCCAGGCGCTGGCCGATCGCATCGCCAACGGC
CAGCTGATCAGGGTGCCGCGCGCGGGCCACCTGGTGCAGGAAGATGCGCCGGAGGCGATCGTCGCGGCGGTGCTCGACGG
GTCAGGGCACGGCTGA

Upstream 100 bases:

>100_bases
GCTCGTGTCGAGTAACCATTTATTTCTACCTATCGGTTTCACACCCGGACAATCCGGAGTAACCTGTTAACAACCAAAAC
AGGTTAGCACGGAGATGCCG

Downstream 100 bases:

>100_bases
CGCCCGCTGTACCGCGCGTCGCTTTTTGCGTGCCGCGCCCCGCGTCAGAACACCGGCACGCCCTGCGCGAGCGTCGAAAT
GAACTTCGCGGTGCGCGGAT

Product: Alpha/beta hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MLELANRFIFEGHRIAWGTLGEGPPLVLVHGTPFSSQVWRRIAPWLARRHRVFFYDLLGYGQSDMPDADVSLSRQNRLFG
AMLDAWNISRPRVLAHDYGGATVLRAHFLDGVDYADLTLVNPVAITPQGSPFVRHVAQHEAAFTGLPAYAHHALVSAYLD
NAVARPLSDEALSIYRAPWLTPDGQAAFYRQIAQMRQRYIEEVEARYAPPDFPVRIVWGEDDAWIPLEQGQALADRIANG
QLIRVPRAGHLVQEDAPEAIVAAVLDGSGHG

Sequences:

>Translated_271_residues
MLELANRFIFEGHRIAWGTLGEGPPLVLVHGTPFSSQVWRRIAPWLARRHRVFFYDLLGYGQSDMPDADVSLSRQNRLFG
AMLDAWNISRPRVLAHDYGGATVLRAHFLDGVDYADLTLVNPVAITPQGSPFVRHVAQHEAAFTGLPAYAHHALVSAYLD
NAVARPLSDEALSIYRAPWLTPDGQAAFYRQIAQMRQRYIEEVEARYAPPDFPVRIVWGEDDAWIPLEQGQALADRIANG
QLIRVPRAGHLVQEDAPEAIVAAVLDGSGHG
>Mature_271_residues
MLELANRFIFEGHRIAWGTLGEGPPLVLVHGTPFSSQVWRRIAPWLARRHRVFFYDLLGYGQSDMPDADVSLSRQNRLFG
AMLDAWNISRPRVLAHDYGGATVLRAHFLDGVDYADLTLVNPVAITPQGSPFVRHVAQHEAAFTGLPAYAHHALVSAYLD
NAVARPLSDEALSIYRAPWLTPDGQAAFYRQIAQMRQRYIEEVEARYAPPDFPVRIVWGEDDAWIPLEQGQALADRIANG
QLIRVPRAGHLVQEDAPEAIVAAVLDGSGHG

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes mono- and di- chlorinated

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 30116; Mature: 30116

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELANRFIFEGHRIAWGTLGEGPPLVLVHGTPFSSQVWRRIAPWLARRHRVFFYDLLGY
CCCHHHHHHCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCC
GQSDMPDADVSLSRQNRLFGAMLDAWNISRPRVLAHDYGGATVLRAHFLDGVDYADLTLV
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEE
NPVAITPQGSPFVRHVAQHEAAFTGLPAYAHHALVSAYLDNAVARPLSDEALSIYRAPWL
CCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
TPDGQAAFYRQIAQMRQRYIEEVEARYAPPDFPVRIVWGEDDAWIPLEQGQALADRIANG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEHHCCHHHHHHHCCC
QLIRVPRAGHLVQEDAPEAIVAAVLDGSGHG
CEEEECCCCCCHHCCCCHHEEEEEECCCCCC
>Mature Secondary Structure
MLELANRFIFEGHRIAWGTLGEGPPLVLVHGTPFSSQVWRRIAPWLARRHRVFFYDLLGY
CCCHHHHHHCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCC
GQSDMPDADVSLSRQNRLFGAMLDAWNISRPRVLAHDYGGATVLRAHFLDGVDYADLTLV
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEE
NPVAITPQGSPFVRHVAQHEAAFTGLPAYAHHALVSAYLDNAVARPLSDEALSIYRAPWL
CCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
TPDGQAAFYRQIAQMRQRYIEEVEARYAPPDFPVRIVWGEDDAWIPLEQGQALADRIANG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEHHCCHHHHHHHCCC
QLIRVPRAGHLVQEDAPEAIVAAVLDGSGHG
CEEEECCCCCCHHCCCCHHEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10094681; 10735862 [H]