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The map label for this gene is potD [C]

Identifier: 78067963

GI number: 78067963

Start: 3663873

End: 3664928

Strand: Direct

Name: potD [C]

Synonym: Bcep18194_A6494

Alternate gene names: 78067963

Gene position: 3663873-3664928 (Clockwise)

Preceding gene: 78067957

Following gene: 78067964

Centisome position: 99.18

GC content: 65.44

Gene sequence:

>1056_bases
ATGAACGAAGTGCCGGTAAACAAGCGTTTGAACGCATGGGCGGCGGGCGCGGCCCGCTTCGCGCTGGTCGCGGCGGTCGC
GCTCGGCGCGGTGCAGGCGGCGCACGCGAAGACGTCGCTGCTCGTCTATACCGCGCTGGAAGACGAGGCGATGAAGCCCT
ACAAGGACGCGTTCGAGAAAGCGAACCCCGATATCGAGATCCGCTGGGTGCGCGACTCGACCGGCTCGGTCACCGCGAAG
CTGCTCGCGGAGAAGAACAACCCGCGCGCGGACGTCGTGCTCGGCCTCGCCGCGTCGAGCCTGACGCTGCTCGACCTGCA
GGGGATGCTGATGCCGTACACGCCGAAGGGCTTCGACCAGCTCACGCGCAAGTACAGCGACGCCAACACGCCGCCGCACT
GGGTCGGCATGGACGTGTGGGGCGCGACGATCTGCTACAACCGCGTCGCCGCGCAGGCGAAGGGCATCCCGAAGCCGACG
TCGTGGGAAGACCTGACGAAGCCCGTCTATAAAGGCGCGATCGTGATGCCGAGCCCGGTTTCCTCCGGCACCGGCTATCT
CGACGTGACCGCGTGGCTGCAGACCTTCGGCGACGACAAAGGCTGGAAATTCATGGACGCGCTCGACAAGAACGTCGCGC
AATACGTGCACTCGGGCTCGAAGCCCTGCACGCTCGCCGGCACCGGCGAATTCCCGATCGGCATCTCGTTCGAGTTCCGC
GGCCACGAACTGCAGTCGCAGGGCGCGCCGATCGATCTCGTGTTCCCGAAGGAAGGGCTCGGCTGGGACATGGAAGGCAC
TGCGATCATGAAGACGACGAAGCAGCCCGACGCCGCGAAGAAACTGGCCGACTTCATGGCTAGCAAGGATGCGAACCAGA
TCACCGCGCGCTGGTGGGCAATCGTCGCGTATCCGGGCGTCGCACGGAAGCTGGCCGGCATCCCCGACAACTACTCGGAC
CTGCTCGTGAAGAACGACTTCGTGTGGTCGGCGAAGAACCGCCAGTCGATCCTCGACACGTGGCAGAAGCGCTACGGCGC
GAAGGCACAGCAGTAA

Upstream 100 bases:

>100_bases
ACAACATGCAGAGAACAGAAAGCAAAACAACGCAGCAGCAAGAGGGCTGGTCTCCACTCATCCGCCGCGTGCCGCGGCGC
CACAGTCAAGGGGTCTGAAG

Downstream 100 bases:

>100_bases
CGTCGCGATCCGCCGCGGGGCCGCGCCGCGCGCCGCCCCGCGCCACCCTTCCTATCGTCGAATCCGGCCCCCGGGCCGCC
CCAGCATGGAGGCGCGCATG

Product: ABC Fe3+ siderophore transporter, periplasmic ligand binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MNEVPVNKRLNAWAAGAARFALVAAVALGAVQAAHAKTSLLVYTALEDEAMKPYKDAFEKANPDIEIRWVRDSTGSVTAK
LLAEKNNPRADVVLGLAASSLTLLDLQGMLMPYTPKGFDQLTRKYSDANTPPHWVGMDVWGATICYNRVAAQAKGIPKPT
SWEDLTKPVYKGAIVMPSPVSSGTGYLDVTAWLQTFGDDKGWKFMDALDKNVAQYVHSGSKPCTLAGTGEFPIGISFEFR
GHELQSQGAPIDLVFPKEGLGWDMEGTAIMKTTKQPDAAKKLADFMASKDANQITARWWAIVAYPGVARKLAGIPDNYSD
LLVKNDFVWSAKNRQSILDTWQKRYGAKAQQ

Sequences:

>Translated_351_residues
MNEVPVNKRLNAWAAGAARFALVAAVALGAVQAAHAKTSLLVYTALEDEAMKPYKDAFEKANPDIEIRWVRDSTGSVTAK
LLAEKNNPRADVVLGLAASSLTLLDLQGMLMPYTPKGFDQLTRKYSDANTPPHWVGMDVWGATICYNRVAAQAKGIPKPT
SWEDLTKPVYKGAIVMPSPVSSGTGYLDVTAWLQTFGDDKGWKFMDALDKNVAQYVHSGSKPCTLAGTGEFPIGISFEFR
GHELQSQGAPIDLVFPKEGLGWDMEGTAIMKTTKQPDAAKKLADFMASKDANQITARWWAIVAYPGVARKLAGIPDNYSD
LLVKNDFVWSAKNRQSILDTWQKRYGAKAQQ
>Mature_351_residues
MNEVPVNKRLNAWAAGAARFALVAAVALGAVQAAHAKTSLLVYTALEDEAMKPYKDAFEKANPDIEIRWVRDSTGSVTAK
LLAEKNNPRADVVLGLAASSLTLLDLQGMLMPYTPKGFDQLTRKYSDANTPPHWVGMDVWGATICYNRVAAQAKGIPKPT
SWEDLTKPVYKGAIVMPSPVSSGTGYLDVTAWLQTFGDDKGWKFMDALDKNVAQYVHSGSKPCTLAGTGEFPIGISFEFR
GHELQSQGAPIDLVFPKEGLGWDMEGTAIMKTTKQPDAAKKLADFMASKDANQITARWWAIVAYPGVARKLAGIPDNYSD
LLVKNDFVWSAKNRQSILDTWQKRYGAKAQQ

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38374; Mature: 38374

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVPVNKRLNAWAAGAARFALVAAVALGAVQAAHAKTSLLVYTALEDEAMKPYKDAFEK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHCCHHHHHHHH
ANPDIEIRWVRDSTGSVTAKLLAEKNNPRADVVLGLAASSLTLLDLQGMLMPYTPKGFDQ
CCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEEEHHCCEEEEECCCCCCCCCCCCHHH
LTRKYSDANTPPHWVGMDVWGATICYNRVAAQAKGIPKPTSWEDLTKPVYKGAIVMPSPV
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEECCCC
SSGTGYLDVTAWLQTFGDDKGWKFMDALDKNVAQYVHSGSKPCTLAGTGEFPIGISFEFR
CCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEC
GHELQSQGAPIDLVFPKEGLGWDMEGTAIMKTTKQPDAAKKLADFMASKDANQITARWWA
CCCHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHEEEEE
IVAYPGVARKLAGIPDNYSDLLVKNDFVWSAKNRQSILDTWQKRYGAKAQQ
EEECCCHHHHHCCCCCCCHHHEEECCEEECCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNEVPVNKRLNAWAAGAARFALVAAVALGAVQAAHAKTSLLVYTALEDEAMKPYKDAFEK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHCCHHHHHHHH
ANPDIEIRWVRDSTGSVTAKLLAEKNNPRADVVLGLAASSLTLLDLQGMLMPYTPKGFDQ
CCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEEEHHCCEEEEECCCCCCCCCCCCHHH
LTRKYSDANTPPHWVGMDVWGATICYNRVAAQAKGIPKPTSWEDLTKPVYKGAIVMPSPV
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEECCCC
SSGTGYLDVTAWLQTFGDDKGWKFMDALDKNVAQYVHSGSKPCTLAGTGEFPIGISFEFR
CCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEC
GHELQSQGAPIDLVFPKEGLGWDMEGTAIMKTTKQPDAAKKLADFMASKDANQITARWWA
CCCHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHEEEEE
IVAYPGVARKLAGIPDNYSDLLVKNDFVWSAKNRQSILDTWQKRYGAKAQQ
EEECCCHHHHHCCCCCCCHHHEEECCEEECCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]