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The map label for this gene is rsmB [H]

Identifier: 78067943

GI number: 78067943

Start: 3641864

End: 3643255

Strand: Reverse

Name: rsmB [H]

Synonym: Bcep18194_A6474

Alternate gene names: 78067943

Gene position: 3643255-3641864 (Counterclockwise)

Preceding gene: 78067944

Following gene: 78067942

Centisome position: 98.62

GC content: 70.91

Gene sequence:

>1392_bases
ATGACACGAACCCGTTCTACCGCTCCGTCTTCTTCCAGCCGCCCGGCGCGCCTGTCCGCGCTGCATCTCGCGCCCGATTC
GCTCGGCTTCGCCCTCGATGCGGCCGCGCAGGCGGTCGATGCGGTACGGCGCGGCACCGCGCTGCCGGCGGCACTCTCGG
CGGTATTCGCGCAGATGCCATCCGGTGCGCAGGCACTCGCGCGTGGGGCAACACAGGACGTCGCATACCGGACGATGCGT
CGCCTCGGCAGCGCGGACTGGCTGATCGGCCGGTTCATCAGCAAGGCGCCGCCCGCGCACGTGCACGCGGTGCTCGCCGG
TGCGTTCGCGCTGCTGCTCGACCCGGCGGACGAGGCCGCCTATCCGGCGTTCACGGTGGTCGACCAGGCCGTGACCGTGA
TCGGCGCACGCCGCGAATGCGCGTTCGCGAAGGGGATGGTCAACGCGGTGCTGCGCCGTTTCCTGCGCGAGCGCGACGCA
CTCGTCGCGGCGCTGCAGGACGATGTCGTCGCACGCTGGAACTACCGGGCGTGGTGGGTCGATTCGGTGAAGCGCGCATG
GCCCGACGCATGGCAGGCGATCCTCGCGGCCGGCGAGCGGCAGGGGCCGCTGACGCTGCGCGTGAACGCGCGCCGCGCGA
GCGTCGACGCGTATCTCGATACGCTGCGCGCGAACGGGATCGAAGCGACCGCGATCGGCCGGCATGCAGTGCGGCTCGCG
TCGGCGCTGCCGGTCGATCGCATTCCGGGGTTTGCCGACGGCGTCGTGTCGGTGCAGGACGCCGGCGCGCAGCTCGCAGC
CGAATGGCTCGGTGCGCGCGACGGCATGCGCGTGCTCGACGCATGCGCGGCGCCCGGCGGCAAGACCGGCCACATTCTCG
AACTGGCCGATGCGGAAGTCGTCGCGCTGGAGAGCGATGCGACACGCGCGGCGCGGATCGGCGAGAACCTCGTGCGGCTG
TCGCTCGAAGCGGACGTGCGTGTCGGCGATGCGGGCGCACCCGGCGCGTGGTACGACGGGCGCCCGTTCGACCGCATCCT
CGCCGATGTGCCGTGCTCGGCGTCCGGCATCGTGCGTCGGCATCCCGACATTCGCTGGCTGCGCCGCGAGGCCGACATCC
CGGCGCTCGTCGCGGAACAGCGCCGCATCCTGTCGGCGTTGTGGCCGCTCGTGAAGCCGGGCGGCGAACTGCTTTACGTG
ACCTGTTCGGTGTTCCCCGAAGAAGGTGAATTGCAGGCCCGCTGGTTTGAAGCGGCCTGTGAAGATGCGGTACGATTGGA
CGCCCCCGGCCAGCTGCTGCCGGGCGGAGTGCAGGGAGGGGCGGCCGCCGGCGCACTCGACCAGAACACCGATCACGACG
GATTTTTCTACGCGCAGTTTCAGAAACGGTGA

Upstream 100 bases:

>100_bases
CCGGTTTGGGATCGCAGGGCCCGCAGGCACCGCGATCCCGCCGTTTGCCGCATTTATTTGCTGTGCCCGCTTGCCGCGCT
TGTTTGCCGAATCTGCTCCG

Downstream 100 bases:

>100_bases
CGATCAAACACCTTTTTCCACTTCGGCTCGCGGCCGTCCTGATGGTCGCGTTGACGCTGTGCCTGGCCCTCGTCCGGCCG
GCGCATGCCGAGTCGATCGC

Product: Fmu, rRNA SAM-dependent methyltransferase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTRTRSTAPSSSSRPARLSALHLAPDSLGFALDAAAQAVDAVRRGTALPAALSAVFAQMPSGAQALARGATQDVAYRTMR
RLGSADWLIGRFISKAPPAHVHAVLAGAFALLLDPADEAAYPAFTVVDQAVTVIGARRECAFAKGMVNAVLRRFLRERDA
LVAALQDDVVARWNYRAWWVDSVKRAWPDAWQAILAAGERQGPLTLRVNARRASVDAYLDTLRANGIEATAIGRHAVRLA
SALPVDRIPGFADGVVSVQDAGAQLAAEWLGARDGMRVLDACAAPGGKTGHILELADAEVVALESDATRAARIGENLVRL
SLEADVRVGDAGAPGAWYDGRPFDRILADVPCSASGIVRRHPDIRWLRREADIPALVAEQRRILSALWPLVKPGGELLYV
TCSVFPEEGELQARWFEAACEDAVRLDAPGQLLPGGVQGGAAAGALDQNTDHDGFFYAQFQKR

Sequences:

>Translated_463_residues
MTRTRSTAPSSSSRPARLSALHLAPDSLGFALDAAAQAVDAVRRGTALPAALSAVFAQMPSGAQALARGATQDVAYRTMR
RLGSADWLIGRFISKAPPAHVHAVLAGAFALLLDPADEAAYPAFTVVDQAVTVIGARRECAFAKGMVNAVLRRFLRERDA
LVAALQDDVVARWNYRAWWVDSVKRAWPDAWQAILAAGERQGPLTLRVNARRASVDAYLDTLRANGIEATAIGRHAVRLA
SALPVDRIPGFADGVVSVQDAGAQLAAEWLGARDGMRVLDACAAPGGKTGHILELADAEVVALESDATRAARIGENLVRL
SLEADVRVGDAGAPGAWYDGRPFDRILADVPCSASGIVRRHPDIRWLRREADIPALVAEQRRILSALWPLVKPGGELLYV
TCSVFPEEGELQARWFEAACEDAVRLDAPGQLLPGGVQGGAAAGALDQNTDHDGFFYAQFQKR
>Mature_462_residues
TRTRSTAPSSSSRPARLSALHLAPDSLGFALDAAAQAVDAVRRGTALPAALSAVFAQMPSGAQALARGATQDVAYRTMRR
LGSADWLIGRFISKAPPAHVHAVLAGAFALLLDPADEAAYPAFTVVDQAVTVIGARRECAFAKGMVNAVLRRFLRERDAL
VAALQDDVVARWNYRAWWVDSVKRAWPDAWQAILAAGERQGPLTLRVNARRASVDAYLDTLRANGIEATAIGRHAVRLAS
ALPVDRIPGFADGVVSVQDAGAQLAAEWLGARDGMRVLDACAAPGGKTGHILELADAEVVALESDATRAARIGENLVRLS
LEADVRVGDAGAPGAWYDGRPFDRILADVPCSASGIVRRHPDIRWLRREADIPALVAEQRRILSALWPLVKPGGELLYVT
CSVFPEEGELQARWFEAACEDAVRLDAPGQLLPGGVQGGAAAGALDQNTDHDGFFYAQFQKR

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=342, Percent_Identity=26.3157894736842, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI76150623, Length=342, Percent_Identity=26.3157894736842, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI32698918, Length=222, Percent_Identity=30.1801801801802, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI2367212, Length=443, Percent_Identity=38.1489841986456, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI87081985, Length=289, Percent_Identity=31.4878892733564, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17536757, Length=269, Percent_Identity=30.4832713754647, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324268, Length=343, Percent_Identity=27.4052478134111, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319447, Length=210, Percent_Identity=30.952380952381, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI22024126, Length=282, Percent_Identity=28.0141843971631, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24668781, Length=240, Percent_Identity=35, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356513, Length=235, Percent_Identity=34.8936170212766, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 49438; Mature: 49306

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTRSTAPSSSSRPARLSALHLAPDSLGFALDAAAQAVDAVRRGTALPAALSAVFAQMP
CCCCCCCCCCCCCCCCEEEEEEECCCHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
SGAQALARGATQDVAYRTMRRLGSADWLIGRFISKAPPAHVHAVLAGAFALLLDPADEAA
CCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHC
YPAFTVVDQAVTVIGARRECAFAKGMVNAVLRRFLRERDALVAALQDDVVARWNYRAWWV
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEH
DSVKRAWPDAWQAILAAGERQGPLTLRVNARRASVDAYLDTLRANGIEATAIGRHAVRLA
HHHHHHCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SALPVDRIPGFADGVVSVQDAGAQLAAEWLGARDGMRVLDACAAPGGKTGHILELADAEV
HHCCHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEECCCEE
VALESDATRAARIGENLVRLSLEADVRVGDAGAPGAWYDGRPFDRILADVPCSASGIVRR
EEECCCCHHHHHHCCHHEEEEEEECEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHC
HPDIRWLRREADIPALVAEQRRILSALWPLVKPGGELLYVTCSVFPEEGELQARWFEAAC
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHH
EDAVRLDAPGQLLPGGVQGGAAAGALDQNTDHDGFFYAQFQKR
HHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TRTRSTAPSSSSRPARLSALHLAPDSLGFALDAAAQAVDAVRRGTALPAALSAVFAQMP
CCCCCCCCCCCCCCCEEEEEEECCCHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
SGAQALARGATQDVAYRTMRRLGSADWLIGRFISKAPPAHVHAVLAGAFALLLDPADEAA
CCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHC
YPAFTVVDQAVTVIGARRECAFAKGMVNAVLRRFLRERDALVAALQDDVVARWNYRAWWV
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEH
DSVKRAWPDAWQAILAAGERQGPLTLRVNARRASVDAYLDTLRANGIEATAIGRHAVRLA
HHHHHHCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SALPVDRIPGFADGVVSVQDAGAQLAAEWLGARDGMRVLDACAAPGGKTGHILELADAEV
HHCCHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEECCCEE
VALESDATRAARIGENLVRLSLEADVRVGDAGAPGAWYDGRPFDRILADVPCSASGIVRR
EEECCCCHHHHHHCCHHEEEEEEECEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHC
HPDIRWLRREADIPALVAEQRRILSALWPLVKPGGELLYVTCSVFPEEGELQARWFEAAC
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHH
EDAVRLDAPGQLLPGGVQGGAAAGALDQNTDHDGFFYAQFQKR
HHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA