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The map label for this gene is ttcA [H]

Identifier: 78067798

GI number: 78067798

Start: 3494221

End: 3495216

Strand: Direct

Name: ttcA [H]

Synonym: Bcep18194_A6329

Alternate gene names: 78067798

Gene position: 3494221-3495216 (Clockwise)

Preceding gene: 78067797

Following gene: 78067799

Centisome position: 94.59

GC content: 65.66

Gene sequence:

>996_bases
ATGAACGCCCCCCACATGAATGACACGACGGCCGATGCCGCCACCCTCGACGCGACCGCCGCCCCGGCCGGCCGCCCGGC
GCTGACGCGCCGCGAACAGAAGGACGCGTACGAGAACAACAAGCTGTTCAAGCGGATCGTGCGCCAGGTCGGCCAGGCGA
TCGGCGACTTCAACATGATCGAGCAAGGCGACAAGGTGATGGTGTGCCTGTCGGGCGGCAAGGACAGCTATGCGATGCTC
GACGTGCTGCTGCGCCTGCGCGAGCGCGCGCCGATCGATTTCGACATCGTCGCGGTGAACCTCGACCAGAAGCAGCCGGG
CTTCCCCGAGCACGTGCTGCCGGAATACCTGAAGCAGGTCGGCGTGCCGTTCCACATCGAGAACCAGGACACCTACAGCA
TCGTCAAGCGGCTCGTGCCCGAGGGCAAGACGACCTGCTCGCTGTGCTCGCGGCTGCGCCGCGGGATCCTGTACCGCGTG
GCCGGCGAGCTCGGCGCGACCAAGATCGCGCTCGGCCACCACCGCGACGACATCGTGCAGACGCTGCTGCTCAACATGTT
CTACGGCGGCAAGCTGAAGGGAATGCCGCCGAAGCTGCAGTCGGACGACGGCAAGAACATCGTGATCCGCCCGCTCGCGT
ACGTGAAGGAAACCGATCTCGAGAAATACGCGGAGTTGCGCGAATTCCCGATCATCCCGTGCAACCTGTGCGGCAGCCAG
CCGAACCTGAAGCGCGCGGAAATGAAGGCGCTGATCCGCGAATGGGACAAGCGCTTCCCGGGCCGTGTCGAGAACATGTT
CAACGCGCTCGCGAAGGTCGTGCCGTCGCACCTGATGGATACGACGCTGTACCCGTTCCAGTCGCTGCGCGCGTCAGGCG
TTGCCGATCCGCAGGGCGACATCGCGTTCGACGAGGAACCGTGCGCGTCGGGCGACGACACCGCGGCGCCGGGTGGCGCC
CAACCGATCTCGATCGTCCAGTTCGACGACCTGTAA

Upstream 100 bases:

>100_bases
CCGTGCGCGCGGTACGCGTCTGTACCGAGAAACCGGACGCTTATCCGGACTGCGACGCCGTCGGCGTCGAAGTCTTTCGC
ATTAAGGACGAGGAGCGCGC

Downstream 100 bases:

>100_bases
GCGGGGCCCGAACACCCGTCGGAACGGGGCCGCAAGGCCTCTTTCCGGCGCTGTCGCCCCGCCGCGACAGGCCCGGAACG
GCGGGGGCCGCATGCTAAAA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNAPHMNDTTADAATLDATAAPAGRPALTRREQKDAYENNKLFKRIVRQVGQAIGDFNMIEQGDKVMVCLSGGKDSYAML
DVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLKQVGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRV
AGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
PNLKRAEMKALIREWDKRFPGRVENMFNALAKVVPSHLMDTTLYPFQSLRASGVADPQGDIAFDEEPCASGDDTAAPGGA
QPISIVQFDDL

Sequences:

>Translated_331_residues
MNAPHMNDTTADAATLDATAAPAGRPALTRREQKDAYENNKLFKRIVRQVGQAIGDFNMIEQGDKVMVCLSGGKDSYAML
DVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLKQVGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRV
AGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
PNLKRAEMKALIREWDKRFPGRVENMFNALAKVVPSHLMDTTLYPFQSLRASGVADPQGDIAFDEEPCASGDDTAAPGGA
QPISIVQFDDL
>Mature_331_residues
MNAPHMNDTTADAATLDATAAPAGRPALTRREQKDAYENNKLFKRIVRQVGQAIGDFNMIEQGDKVMVCLSGGKDSYAML
DVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLKQVGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRV
AGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
PNLKRAEMKALIREWDKRFPGRVENMFNALAKVVPSHLMDTTLYPFQSLRASGVADPQGDIAFDEEPCASGDDTAAPGGA
QPISIVQFDDL

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=279, Percent_Identity=66.3082437275986, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI193211090, Length=243, Percent_Identity=30.8641975308642, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17540090, Length=262, Percent_Identity=27.8625954198473, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24651972, Length=278, Percent_Identity=28.7769784172662, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 36710; Mature: 36710

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPHMNDTTADAATLDATAAPAGRPALTRREQKDAYENNKLFKRIVRQVGQAIGDFNMI
CCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EQGDKVMVCLSGGKDSYAMLDVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLKQV
CCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHC
GVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRDDIVQ
CCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
TLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
HHHHHHHCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHCCCCC
PNLKRAEMKALIREWDKRFPGRVENMFNALAKVVPSHLMDTTLYPFQSLRASGVADPQGD
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IAFDEEPCASGDDTAAPGGAQPISIVQFDDL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MNAPHMNDTTADAATLDATAAPAGRPALTRREQKDAYENNKLFKRIVRQVGQAIGDFNMI
CCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EQGDKVMVCLSGGKDSYAMLDVLLRLRERAPIDFDIVAVNLDQKQPGFPEHVLPEYLKQV
CCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHC
GVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRDDIVQ
CCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
TLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYVKETDLEKYAELREFPIIPCNLCGSQ
HHHHHHHCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHCCCCC
PNLKRAEMKALIREWDKRFPGRVENMFNALAKVVPSHLMDTTLYPFQSLRASGVADPQGD
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IAFDEEPCASGDDTAAPGGAQPISIVQFDDL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA