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The map label for this gene is metH [H]

Identifier: 78067728

GI number: 78067728

Start: 3416584

End: 3417702

Strand: Direct

Name: metH [H]

Synonym: Bcep18194_A6259

Alternate gene names: 78067728

Gene position: 3416584-3417702 (Clockwise)

Preceding gene: 78067727

Following gene: 78067729

Centisome position: 92.49

GC content: 66.49

Gene sequence:

>1119_bases
ATGCGCACCGGCTACAATGACGGCTTTCCCGTCTTCGCCGGTCTTTCGCCCATGTCTGCGACTCCTCTCGCCGCTTCCGT
CCCGCTCGACGCGCCCTACACGCGCGGCGCCGCTCTGCCTGCGCTGCTGAAATCGCGCATCCTGATCCTCGACGGCGCGA
TGGGCACGATGATCCAGCGCTACAAGCTCGACGAAGCCGCGTATCGCGGCGAGCGCTTCAAGGATTTCCCGCGCGACATC
AAGGGCAACAACGAGTTGCTGTCGATCACGCAGCCGCAGATCATCCGCGAGATCCACGACCAGTACTTCGCGGCCGGCGC
CGACATCGTCGAGACCAACACGTTCGGCGCGACGACCGTCGCGCAATCCGACTACGAGATGGAAGATCTCGTCGTCGAGA
TGAACATCGAATCGGCGAAGCTCGCGCGCGAAGCGGCCGAGAAATACGCGACGCCGGAAAAGCCGCGCTTCGTCGCGGGC
GCGATCGGGCCGACGCCGAAGACGGCCAGCATCTCGCCGGACGTCAACGACCCGGGCGCACGCAACGTCACGTTCGACGA
GCTGCGCGCGTCGTACTACCAGCAGGCGAAGGCGCTGCTCGACGGCGGCGTCGACCTGTTCCTCGTCGAGACGATCTTCG
ACACGCTGAACGCGAAGGCCGCACTGTTCGCGCTCGACGAGCTGTTCGAAGACACCGGCGAGCGCCTGCCGATCATGATC
TCGGGCACCGTCACCGATGCGTCGGGCCGGATTTTGTCGGGCCAGACGGTCGAGGCGTTCTGGAATTCGCTGCGTCATGC
GAAGCCGCTCACGTTCGGCCTGAACTGCGCGCTCGGCGCGGCGCTGATGCGCCCGTACATCGCCGAGCTCGCGAAGCTGT
GCGACACGTACGTGTCGTGCTACCCGAACGCGGGCCTGCCGAACCCGATGGCCGAGACGGGCTTCGACGAGACGCCGGAC
GTCACGTCGGGCCTCCTGAAGGAATTCGCGCAAGCCGGGCTCGTGAACCTCGCGGGCGGCTGCTGCGGCACGACGCCCGA
ACACATCGCCGAGATCGCGAAGGCGCTCGCCGACGTGAAGCCGCGCCGCTGGCCGAACCAGTACAGCGACAACGCCTGA

Upstream 100 bases:

>100_bases
CGCGGCGCCGCGAGCACTCCGATCATGCCCCCGCCCGGTTCGCCGGCGGGGGTTTTCATTTGGCGGCCCGGCCGGCGCGG
CGCAAGGCCGGGCGGTGCCC

Downstream 100 bases:

>100_bases
CCCGACCGCCCCACGTCCTTCATCACCAGAATCCGCACCGCCATGACCGATCACATGATGCGCCTCGCCGGCCTCGAGCC
GTTCAACGTCACGCCCGGGA

Product: methionine synthase (B12-dependent)

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRTGYNDGFPVFAGLSPMSATPLAASVPLDAPYTRGAALPALLKSRILILDGAMGTMIQRYKLDEAAYRGERFKDFPRDI
KGNNELLSITQPQIIREIHDQYFAAGADIVETNTFGATTVAQSDYEMEDLVVEMNIESAKLAREAAEKYATPEKPRFVAG
AIGPTPKTASISPDVNDPGARNVTFDELRASYYQQAKALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMI
SGTVTDASGRILSGQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMAETGFDETPD
VTSGLLKEFAQAGLVNLAGGCCGTTPEHIAEIAKALADVKPRRWPNQYSDNA

Sequences:

>Translated_372_residues
MRTGYNDGFPVFAGLSPMSATPLAASVPLDAPYTRGAALPALLKSRILILDGAMGTMIQRYKLDEAAYRGERFKDFPRDI
KGNNELLSITQPQIIREIHDQYFAAGADIVETNTFGATTVAQSDYEMEDLVVEMNIESAKLAREAAEKYATPEKPRFVAG
AIGPTPKTASISPDVNDPGARNVTFDELRASYYQQAKALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMI
SGTVTDASGRILSGQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMAETGFDETPD
VTSGLLKEFAQAGLVNLAGGCCGTTPEHIAEIAKALADVKPRRWPNQYSDNA
>Mature_372_residues
MRTGYNDGFPVFAGLSPMSATPLAASVPLDAPYTRGAALPALLKSRILILDGAMGTMIQRYKLDEAAYRGERFKDFPRDI
KGNNELLSITQPQIIREIHDQYFAAGADIVETNTFGATTVAQSDYEMEDLVVEMNIESAKLAREAAEKYATPEKPRFVAG
AIGPTPKTASISPDVNDPGARNVTFDELRASYYQQAKALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMI
SGTVTDASGRILSGQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMAETGFDETPD
VTSGLLKEFAQAGLVNLAGGCCGTTPEHIAEIAKALADVKPRRWPNQYSDNA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=328, Percent_Identity=58.2317073170732, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1790450, Length=329, Percent_Identity=59.2705167173252, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1786456, Length=329, Percent_Identity=27.0516717325228, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17535405, Length=330, Percent_Identity=54.5454545454545, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24585077, Length=289, Percent_Identity=23.5294117647059, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 40176; Mature: 40176

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTGYNDGFPVFAGLSPMSATPLAASVPLDAPYTRGAALPALLKSRILILDGAMGTMIQR
CCCCCCCCCCCEECCCCCCCCCCEECCCCCCCCCCCHHHHHHHHCCEEEEECHHHHHHHH
YKLDEAAYRGERFKDFPRDIKGNNELLSITQPQIIREIHDQYFAAGADIVETNTFGATTV
HHHHHHHHCCCHHHHCCHHCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCEE
AQSDYEMEDLVVEMNIESAKLAREAAEKYATPEKPRFVAGAIGPTPKTASISPDVNDPGA
ECCCCCHHHEEEEECCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCC
RNVTFDELRASYYQQAKALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMI
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEE
SGTVTDASGRILSGQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSC
EEEEECCCCCEECCCHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPNAGLPNPMAETGFDETPDVTSGLLKEFAQAGLVNLAGGCCGTTPEHIAEIAKALADVK
CCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCC
PRRWPNQYSDNA
CCCCCCCCCCCC
>Mature Secondary Structure
MRTGYNDGFPVFAGLSPMSATPLAASVPLDAPYTRGAALPALLKSRILILDGAMGTMIQR
CCCCCCCCCCCEECCCCCCCCCCEECCCCCCCCCCCHHHHHHHHCCEEEEECHHHHHHHH
YKLDEAAYRGERFKDFPRDIKGNNELLSITQPQIIREIHDQYFAAGADIVETNTFGATTV
HHHHHHHHCCCHHHHCCHHCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCEE
AQSDYEMEDLVVEMNIESAKLAREAAEKYATPEKPRFVAGAIGPTPKTASISPDVNDPGA
ECCCCCHHHEEEEECCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCC
RNVTFDELRASYYQQAKALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMI
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEE
SGTVTDASGRILSGQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSC
EEEEECCCCCEECCCHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPNAGLPNPMAETGFDETPDVTSGLLKEFAQAGLVNLAGGCCGTTPEHIAEIAKALADVK
CCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCC
PRRWPNQYSDNA
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]