| Definition | Burkholderia sp. 383 chromosome 1, complete genome. |
|---|---|
| Accession | NC_007510 |
| Length | 3,694,126 |
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The map label for this gene is metH [H]
Identifier: 78067729
GI number: 78067729
Start: 3417757
End: 3420462
Strand: Direct
Name: metH [H]
Synonym: Bcep18194_A6260
Alternate gene names: 78067729
Gene position: 3417757-3420462 (Clockwise)
Preceding gene: 78067728
Following gene: 78067731
Centisome position: 92.52
GC content: 65.34
Gene sequence:
>2706_bases ATGATGCGCCTCGCCGGCCTCGAGCCGTTCAACGTCACGCCCGGGACGCTCTTCATCAACGTCGGTGAACGCACCAACGT CACCGGCTCGAAGGCGTTCGCACGCATGATCCTCAACGGCCAGTTCGACGAGGCTCTCGCGGTCGCGCGCCAGCAGGTCG AGAACGGCGCGCAGGTGATCGACATCAACATGGACGAGGCGATGCTCGATTCGAAGGCGGCGATGGTGCGCTTCCTGAAC CTGATCGCATCGGAGCCGGACATCGCGCGCGTGCCGATCATGATCGACTCGTCAAAGTGGGAAGTGATCGAGGCGGGCCT GAAGTGCGTGCAGGGCAAGGCGATCGTGAACTCGATCTCGCTGAAGGAAGGCGAGGACGCGTTCCGCCACCACGCGCACC TGATCCGCCGCTACGGCGCGGCGGCCGTCGTGATGGCGTTCGACGAAACCGGCCAGGCCGATACCTACGAGCGCAAGATC GAGATCTGCACGCGCTCGTACAACTTCCTCGTGAACGAAGTCGGCTTCCCGCCGGAAGACATCATCTTCGACCCGAACAT CTTCGCGATCGCGACCGGCATCGAGGAGCACAACAACTACGCGGTCGACTTCATCGAGGCGACCCGCTGGATCAAGCAAA ACCTGCCGCACGCGAAGATCAGCGGCGGCGTGTCGAACGTGTCGTTCTCGTTCCGCGGCAACGACCCGGTGCGCGAGGCG ATCCACACCGTGTTCCTGTACCACGCGATCCAGGCGGGGATGGACATGGGCATCGTCAACGCGGGCCAGCTCGGCGTGTA CGCCGACCTCGACCCGGAGCTGCGCGAGCGCGTCGAGGACGTGATCCTGAACCGCCGCGACGATTCGACCGACCGCCTGC TCGAAATCGCCGACAAGTTCAAGGCGGGCGGCGCGAAGAAGGAAGAGAACCTCGAGTGGCGCAACCAGCCGGTCGAGAAG CGGCTCTCGCATGCACTGGTGCACGGCATCACGAACTTCATCGTCGAGGATACCGAGGAAGTGCGCGCGAAGATCGACGC GGCCGGCGGCCGCCCGATCAACGTGATCGAAGGGCCGCTGATGGACGGGATGAACATCGTCGGCGACCTGTTCGGCCAGG GCAAGATGTTCCTGCCGCAGGTCGTGAAATCGGCGCGCGTGATGAAGCAGGCCGTCGCGCACCTGATTCCGTTCATCGAG GAAGAAAAGCGCCTGCTCGCGGAAGCGGGCGGCGACGTGCGCGCGAAGGGCAAGATCGTCATCGCGACCGTGAAGGGCGA CGTGCACGACATCGGCAAGAACATCGTGTCGGTCGTGCTCCAGTGCAACAACTTCGAAGTCGTCAACATGGGCGTGATGG TCCCGTGCAACGAGATCCTCGCGAAGGCGAAGGTCGAGGGCGCGGACATCATCGGGCTGTCGGGCCTCATCACGCCGAGC CTCGAGGAAATGGCGTACGTCGCGTCCGAAATGCAGCGCGACGACTACTTCCGCGTGAAGAAGATTCCGCTGCTGATCGG CGGCGCGACGACCTCGCGCGTGCACACGGCCGTGAAGATCGCGCCGCACTACGAAGGCCCGGTGGTCTACGTGCCGGACG CGTCGCGCTCGGTATCGGTCGCCTCCAGCCTGCTGTCCGACGAAGGCGCGACGAAGTACCTCGACGAGCTGAAGTCCGAC TACGAACGCATCCGCGACCAGCACGCGAACCGCAAGGCGCAGCCGATGGTCACGCTCGCCGAAGCGCGCGCGAACAAGAC CAAGGTCGACTGGGCGGGCTACCAGCCGGTGAAGCCGAAGTTCATCGGCCGCCGCGTGTTCAAGAACTACGACCTGAACG AGCTCGCGCAGTACATCGACTGGGGCCCGTTCTTCCAGACCTGGGATCTCGCGGGCCCGTACCCTGCGATCCTGAACGAC GAGATCGTCGGCGAATCGGCGCGGCGCGTGTTTTCCGACGCAAAATCGATGCTCGCGCGCCTGATCCAGGGCCGCTGGCT GACCGCGAGCGGCGTGATCTCGCTGCTGCCGGCGAATACCGTCAACGGCGACGACATCGAGATCTACAGCGACGAGTCGC GCTCGGAAGTGCTGCTCACGTGGCGCAACCTGCGCCAGCAGAGCGTGCGCCCGGTGGTCGACGGCGTAATGCGGCCGAAC CGCTCGCTCGCCGACTTCATCGCGCCGAAGGAATCGGGCGTCGCCGACTACATCGGGATGTTCGCGGTGACGGCCGGCCT CGGCGTCGACGTGAAGGAAAAGCAGTTCGAAGCCGACCACGACGACTACAGCGCGATCATGCTGAAGGCGCTCGCCGACC GCTTCGCGGAAGCCTTCGCGGAAGCGATGCATGCGCGCGTGCGGCGCGAGCTGTGGGGCTACGCGAGCGGCGAAACGCTC GACAACGATGCGCTGATCGCCGAAAAGTACGCGGGCATCCGCCCGGCGCCCGGCTACCCGGCCTGCCCCGACCACCTCGT GAAGCGCGACATGTTCGACGTGCTGCGCGCGGACGAGATCGGCATGAGCGTGACCGATTCGCTGGCGATGCTGCCGGCCG CGAGCGTGTCGGGCTTCTATCTCGCGCATCCGGACAGCACGTACTTCTCGGTCGGCAAGATCGGGCAGGATCAGCTCGAG GACTATGCGCAGCGCATGGCGCTGTCGCTCGACGACGCGCGCCGCGCGCTTGCGCCGCAGCTGTAA
Upstream 100 bases:
>100_bases CGTGAAGCCGCGCCGCTGGCCGAACCAGTACAGCGACAACGCCTGACCCGACCGCCCCACGTCCTTCATCACCAGAATCC GCACCGCCATGACCGATCAC
Downstream 100 bases:
>100_bases TCGGCCCTTCGACGGGCGCGCAGCCGCCCGCTATTGTCAGACAAAAGAAAAAGCCCGCTCGGAAGCGGGCTTTTTCCTAC CTTTCGATCCTTTCGGACCG
Product: methionine synthase (B12-dependent)
Products: NA
Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]
Number of amino acids: Translated: 901; Mature: 901
Protein sequence:
>901_residues MMRLAGLEPFNVTPGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMVRFLN LIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEDAFRHHAHLIRRYGAAAVVMAFDETGQADTYERKI EICTRSYNFLVNEVGFPPEDIIFDPNIFAIATGIEEHNNYAVDFIEATRWIKQNLPHAKISGGVSNVSFSFRGNDPVREA IHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRDDSTDRLLEIADKFKAGGAKKEENLEWRNQPVEK RLSHALVHGITNFIVEDTEEVRAKIDAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIE EEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGLITPS LEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASSLLSDEGATKYLDELKSD YERIRDQHANRKAQPMVTLAEARANKTKVDWAGYQPVKPKFIGRRVFKNYDLNELAQYIDWGPFFQTWDLAGPYPAILND EIVGESARRVFSDAKSMLARLIQGRWLTASGVISLLPANTVNGDDIEIYSDESRSEVLLTWRNLRQQSVRPVVDGVMRPN RSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFAEAMHARVRRELWGYASGETL DNDALIAEKYAGIRPAPGYPACPDHLVKRDMFDVLRADEIGMSVTDSLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLE DYAQRMALSLDDARRALAPQL
Sequences:
>Translated_901_residues MMRLAGLEPFNVTPGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMVRFLN LIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEDAFRHHAHLIRRYGAAAVVMAFDETGQADTYERKI EICTRSYNFLVNEVGFPPEDIIFDPNIFAIATGIEEHNNYAVDFIEATRWIKQNLPHAKISGGVSNVSFSFRGNDPVREA IHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRDDSTDRLLEIADKFKAGGAKKEENLEWRNQPVEK RLSHALVHGITNFIVEDTEEVRAKIDAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIE EEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGLITPS LEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASSLLSDEGATKYLDELKSD YERIRDQHANRKAQPMVTLAEARANKTKVDWAGYQPVKPKFIGRRVFKNYDLNELAQYIDWGPFFQTWDLAGPYPAILND EIVGESARRVFSDAKSMLARLIQGRWLTASGVISLLPANTVNGDDIEIYSDESRSEVLLTWRNLRQQSVRPVVDGVMRPN RSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFAEAMHARVRRELWGYASGETL DNDALIAEKYAGIRPAPGYPACPDHLVKRDMFDVLRADEIGMSVTDSLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLE DYAQRMALSLDDARRALAPQL >Mature_901_residues MMRLAGLEPFNVTPGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMVRFLN LIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEDAFRHHAHLIRRYGAAAVVMAFDETGQADTYERKI EICTRSYNFLVNEVGFPPEDIIFDPNIFAIATGIEEHNNYAVDFIEATRWIKQNLPHAKISGGVSNVSFSFRGNDPVREA IHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRDDSTDRLLEIADKFKAGGAKKEENLEWRNQPVEK RLSHALVHGITNFIVEDTEEVRAKIDAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIE EEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGLITPS LEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASSLLSDEGATKYLDELKSD YERIRDQHANRKAQPMVTLAEARANKTKVDWAGYQPVKPKFIGRRVFKNYDLNELAQYIDWGPFFQTWDLAGPYPAILND EIVGESARRVFSDAKSMLARLIQGRWLTASGVISLLPANTVNGDDIEIYSDESRSEVLLTWRNLRQQSVRPVVDGVMRPN RSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFAEAMHARVRRELWGYASGETL DNDALIAEKYAGIRPAPGYPACPDHLVKRDMFDVLRADEIGMSVTDSLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLE DYAQRMALSLDDARRALAPQL
Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]
COG id: COG1410
COG function: function code E; Methionine synthase I, cobalamin-binding domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pterin-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=915, Percent_Identity=55.0819672131148, Blast_Score=981, Evalue=0.0, Organism=Escherichia coli, GI1790450, Length=904, Percent_Identity=62.2787610619469, Blast_Score=1101, Evalue=0.0, Organism=Caenorhabditis elegans, GI17535405, Length=912, Percent_Identity=54.4956140350877, Blast_Score=951, Evalue=0.0,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006158 - InterPro: IPR011005 - InterPro: IPR003759 - InterPro: IPR011822 - InterPro: IPR000489 - InterPro: IPR003726 - InterPro: IPR004223 [H]
Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]
EC number: =2.1.1.13 [H]
Molecular weight: Translated: 99523; Mature: 99523
Theoretical pI: Translated: 5.09; Mature: 5.09
Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 3.1 %Met (Translated Protein) 3.7 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 3.1 %Met (Mature Protein) 3.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMRLAGLEPFNVTPGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVI CCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEE DINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSIS EECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHHHHCC LKEGEDAFRHHAHLIRRYGAAAVVMAFDETGQADTYERKIEICTRSYNFLVNEVGFPPED CCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH IIFDPNIFAIATGIEEHNNYAVDFIEATRWIKQNLPHAKISGGVSNVSFSFRGNDPVREA EEECCCEEEEEECCHHCCCEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHH IHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRDDSTDRLLEIADKF HHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH KAGGAKKEENLEWRNQPVEKRLSHALVHGITNFIVEDTEEVRAKIDAAGGRPINVIEGPL HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCC MDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLLAEAGGDVRAKGKIV CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEE IATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGLITPS EEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEEEECCCCCCC LEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSV HHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHHEEEEEEECCCCCCEEECCCCCCHHHH ASSLLSDEGATKYLDELKSDYERIRDQHANRKAQPMVTLAEARANKTKVDWAGYQPVKPK HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCCCCCHH FIGRRVFKNYDLNELAQYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDAKSMLAR HHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHH LIQGRWLTASGVISLLPANTVNGDDIEIYSDESRSEVLLTWRNLRQQSVRPVVDGVMRPN HHCCCCEECCCHHEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC RSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFA CHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH EAMHARVRRELWGYASGETLDNDALIAEKYAGIRPAPGYPACPDHLVKRDMFDVLRADEI HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC GMSVTDSLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLEDYAQRMALSLDDARRALAPQ CCCHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC L C >Mature Secondary Structure MMRLAGLEPFNVTPGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVI CCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEE DINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSIS EECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHHHHCC LKEGEDAFRHHAHLIRRYGAAAVVMAFDETGQADTYERKIEICTRSYNFLVNEVGFPPED CCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH IIFDPNIFAIATGIEEHNNYAVDFIEATRWIKQNLPHAKISGGVSNVSFSFRGNDPVREA EEECCCEEEEEECCHHCCCEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHH IHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRDDSTDRLLEIADKF HHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH KAGGAKKEENLEWRNQPVEKRLSHALVHGITNFIVEDTEEVRAKIDAAGGRPINVIEGPL HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCC MDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLLAEAGGDVRAKGKIV CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEE IATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGLITPS EEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEEEECCCCCCC LEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSV HHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHHEEEEEEECCCCCCEEECCCCCCHHHH ASSLLSDEGATKYLDELKSDYERIRDQHANRKAQPMVTLAEARANKTKVDWAGYQPVKPK HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCCCCCHH FIGRRVFKNYDLNELAQYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDAKSMLAR HHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHH LIQGRWLTASGVISLLPANTVNGDDIEIYSDESRSEVLLTWRNLRQQSVRPVVDGVMRPN HHCCCCEECCCHHEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC RSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFA CHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH EAMHARVRRELWGYASGETLDNDALIAEKYAGIRPAPGYPACPDHLVKRDMFDVLRADEI HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC GMSVTDSLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLEDYAQRMALSLDDARRALAPQ CCCHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC L C
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10952301 [H]