Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 78067611

GI number: 78067611

Start: 3285206

End: 3286852

Strand: Direct

Name: leuA [H]

Synonym: Bcep18194_A6142

Alternate gene names: 78067611

Gene position: 3285206-3286852 (Clockwise)

Preceding gene: 78067610

Following gene: 78067614

Centisome position: 88.93

GC content: 68.25

Gene sequence:

>1647_bases
ATGCAGCGCAATCCGCAAGACAAGTACCGTCCGTTCGAGCCCGTCCGCCTCAATGGCCGCAAATGGCCGTCGCGCACCAT
CGAGCGCGCACCCGTCTGGATGAGCACCGACCTGCGCGACGGCAACCAGTCGCTGATCGAGCCGATGAGCATCGAGCAGA
AGCTCGAATTCTTCGAGATGCTGGTCGCGATCGGGTTCAAGGAGATCGAAGTCGGTTTTCCGTCGGCGTCGCAAACCGAC
TTCGATTTCGTCCGCAAGCTGATCGACGACAAGCGGATTCCCGACGACGTGACGATCGAAGTGCTCGTGCAGGCGCGCGA
AGACCTGATCGCCCGCACGTTCGACGCGCTCGAAGGCGTGCCGCGCGCGATCGTGCACCTGTACAACGCAGTCTGCCCGT
CGTTCCGCCGCATCGTGTTCGGGATGTCGAAGGCCGACGTGAAGGCGCTCGCGATCGACGGCACGCGCATCATCAAGGAA
CACTGCGCCGCGCGCCCCGACACGCAGTGGACCCTCCAGTACTCGCCGGAAACCTTCAGCATGACCGAGCTGACGTTCGC
GCGCGAAATCTGCGACGCCGTCGCGCAGACCTGGCGCCCGACCCGCGATCACAAGATGATCGTCAACCTGCCGGCCACCG
TCGAAGCCGCGGGCCCGAACGTATTCGCCGACCAGATCGAGTGGATGGACCGCAACCTCGCGTATCGCGACAGCATCGTG
CTGTCCGTGCATCCGCACAACGATCGCGGCACCGCGGTCGCGGCAGCCGAACTCGCGCTGCTCGCGGGTGCCGACCGCAT
CGAAGGCTGCCTGTTCGGCAATGGCGAGCGCACCGGCAACGTCGATCTCGTCACGCTCGCGCTGAATCTCTACACGCAGG
GCATCGATCCGGGCCTCGATTTCTCCGACATCGACGCCGTGCGCCGCGTCGTCGAGCGCTGCAACCAGATTCCGGTACAT
CCGCGCCATCCGTACGCGGGCGACCTCGTGTTCACCGCATTCTCGGGTTCGCACCAGGACGCGATCCGCAAGGGCTTCGC
GCAGCAGCGCCCCGACGCGGCCTGGGAAGTGCCGTACCTGCCGATCGACCCGGCCGACCTCGGCCGCAGCTATGACGCGG
TGATCCGCGTGAACAGCCAGTCCGGCAAGGGCGGCGCGACGTTCCTGCTCGAACGCGGGATGGGCTTCACGCCGACGCGC
CGCGTGCAGATCGAATTCAGCCACGCGGTGCAGACGCTCGCCGACGCGTCCGGCGAGGAAGTGACGGGCGACGCGATCTG
CGCGCTGTTCGCGCGCGAATTCTTCGATACCGACGGGCCGGCCACGCGCCACGGCAACGGCGCGCGCTGGCAGAACCGCG
AGATCGCGGCAGCGCCCGTGGCCGATACGACGCCCGACGAGGCCGTGCGACGTTTGGCCGCGGCGTTCGCGGCGGCCGCC
GGCGCGGCGATCGACGTCGCGTCGTGCGAACACGCCCGCACGACGGACGGGCGGATCGCGGTATCGGTCGGCTGCCGGGT
CGGCGATGCGCCGCTGCGGCACGGCGTCGGCCTGCACGCCGATGCGGCGAGCGCCGCACTCGATGCCGTCGTCAGCGCGA
TCAACCGCTCGGCCTGGCACTGCGCGGATCATCGCGCGGCGGCCTGA

Upstream 100 bases:

>100_bases
CCGCATCCGCAGCGCGTTCACGTGCCGCGGAGCGAGGCAGCGCCAACCGTCGCCCATGCCGCCCGTCTTCGCTCGCGACT
TGAGACCCGAGGTCCAGAAG

Downstream 100 bases:

>100_bases
GCGCCGGGCTCCGTTTCAGGGTTCAAACGTCAGCGAGCGGAGCGTGACCGCCCGTGCGCCGCGCGATCCGCGCCGCACGA
GGCAGCAAAAAGCGGCCCAG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MQRNPQDKYRPFEPVRLNGRKWPSRTIERAPVWMSTDLRDGNQSLIEPMSIEQKLEFFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIDDKRIPDDVTIEVLVQAREDLIARTFDALEGVPRAIVHLYNAVCPSFRRIVFGMSKADVKALAIDGTRIIKE
HCAARPDTQWTLQYSPETFSMTELTFAREICDAVAQTWRPTRDHKMIVNLPATVEAAGPNVFADQIEWMDRNLAYRDSIV
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRRVVERCNQIPVH
PRHPYAGDLVFTAFSGSHQDAIRKGFAQQRPDAAWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGATFLLERGMGFTPTR
RVQIEFSHAVQTLADASGEEVTGDAICALFAREFFDTDGPATRHGNGARWQNREIAAAPVADTTPDEAVRRLAAAFAAAA
GAAIDVASCEHARTTDGRIAVSVGCRVGDAPLRHGVGLHADAASAALDAVVSAINRSAWHCADHRAAA

Sequences:

>Translated_548_residues
MQRNPQDKYRPFEPVRLNGRKWPSRTIERAPVWMSTDLRDGNQSLIEPMSIEQKLEFFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIDDKRIPDDVTIEVLVQAREDLIARTFDALEGVPRAIVHLYNAVCPSFRRIVFGMSKADVKALAIDGTRIIKE
HCAARPDTQWTLQYSPETFSMTELTFAREICDAVAQTWRPTRDHKMIVNLPATVEAAGPNVFADQIEWMDRNLAYRDSIV
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRRVVERCNQIPVH
PRHPYAGDLVFTAFSGSHQDAIRKGFAQQRPDAAWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGATFLLERGMGFTPTR
RVQIEFSHAVQTLADASGEEVTGDAICALFAREFFDTDGPATRHGNGARWQNREIAAAPVADTTPDEAVRRLAAAFAAAA
GAAIDVASCEHARTTDGRIAVSVGCRVGDAPLRHGVGLHADAASAALDAVVSAINRSAWHCADHRAAA
>Mature_548_residues
MQRNPQDKYRPFEPVRLNGRKWPSRTIERAPVWMSTDLRDGNQSLIEPMSIEQKLEFFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIDDKRIPDDVTIEVLVQAREDLIARTFDALEGVPRAIVHLYNAVCPSFRRIVFGMSKADVKALAIDGTRIIKE
HCAARPDTQWTLQYSPETFSMTELTFAREICDAVAQTWRPTRDHKMIVNLPATVEAAGPNVFADQIEWMDRNLAYRDSIV
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRRVVERCNQIPVH
PRHPYAGDLVFTAFSGSHQDAIRKGFAQQRPDAAWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGATFLLERGMGFTPTR
RVQIEFSHAVQTLADASGEEVTGDAICALFAREFFDTDGPATRHGNGARWQNREIAAAPVADTTPDEAVRRLAAAFAAAA
GAAIDVASCEHARTTDGRIAVSVGCRVGDAPLRHGVGLHADAASAALDAVVSAINRSAWHCADHRAAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=392, Percent_Identity=24.7448979591837, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324225, Length=446, Percent_Identity=55.8295964125561, Blast_Score=521, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6324682, Length=442, Percent_Identity=54.2986425339367, Blast_Score=512, Evalue=1e-146,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 60034; Mature: 60034

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRNPQDKYRPFEPVRLNGRKWPSRTIERAPVWMSTDLRDGNQSLIEPMSIEQKLEFFEM
CCCCCCCCCCCCCCEEECCCCCCCHHHHCCCEEEECCCCCCCHHHHCCHHHHHHHHHHHH
LVAIGFKEIEVGFPSASQTDFDFVRKLIDDKRIPDDVTIEVLVQAREDLIARTFDALEGV
HHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCH
PRAIVHLYNAVCPSFRRIVFGMSKADVKALAIDGTRIIKEHCAARPDTQWTLQYSPETFS
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEECCCCCC
MTELTFAREICDAVAQTWRPTRDHKMIVNLPATVEAAGPNVFADQIEWMDRNLAYRDSIV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHCCCEEECEEE
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLD
EEEECCCCCCCHHHHHHHHHHHCCHHHCEEEECCCCCCCCEEEEEEEEHHHHCCCCCCCC
FSDIDAVRRVVERCNQIPVHPRHPYAGDLVFTAFSGSHQDAIRKGFAQQRPDAAWEVPYL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
PIDPADLGRSYDAVIRVNSQSGKGGATFLLERGMGFTPTRRVQIEFSHAVQTLADASGEE
CCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHCCCCCC
VTGDAICALFAREFFDTDGPATRHGNGARWQNREIAAAPVADTTPDEAVRRLAAAFAAAA
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHC
GAAIDVASCEHARTTDGRIAVSVGCRVGDAPLRHGVGLHADAASAALDAVVSAINRSAWH
CCEEEECCCCCCCCCCCEEEEEECCEECCCHHHCCCCCCCHHHHHHHHHHHHHHHCCHHH
CADHRAAA
CCCCCCCC
>Mature Secondary Structure
MQRNPQDKYRPFEPVRLNGRKWPSRTIERAPVWMSTDLRDGNQSLIEPMSIEQKLEFFEM
CCCCCCCCCCCCCCEEECCCCCCCHHHHCCCEEEECCCCCCCHHHHCCHHHHHHHHHHHH
LVAIGFKEIEVGFPSASQTDFDFVRKLIDDKRIPDDVTIEVLVQAREDLIARTFDALEGV
HHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCH
PRAIVHLYNAVCPSFRRIVFGMSKADVKALAIDGTRIIKEHCAARPDTQWTLQYSPETFS
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEECCCCCC
MTELTFAREICDAVAQTWRPTRDHKMIVNLPATVEAAGPNVFADQIEWMDRNLAYRDSIV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHCCCEEECEEE
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLD
EEEECCCCCCCHHHHHHHHHHHCCHHHCEEEECCCCCCCCEEEEEEEEHHHHCCCCCCCC
FSDIDAVRRVVERCNQIPVHPRHPYAGDLVFTAFSGSHQDAIRKGFAQQRPDAAWEVPYL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
PIDPADLGRSYDAVIRVNSQSGKGGATFLLERGMGFTPTRRVQIEFSHAVQTLADASGEE
CCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHCCCCCC
VTGDAICALFAREFFDTDGPATRHGNGARWQNREIAAAPVADTTPDEAVRRLAAAFAAAA
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHC
GAAIDVASCEHARTTDGRIAVSVGCRVGDAPLRHGVGLHADAASAALDAVVSAINRSAWH
CCEEEECCCCCCCCCCCEEEEEECCEECCCHHHCCCCCCCHHHHHHHHHHHHHHHCCHHH
CADHRAAA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA