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The map label for this gene is rpoH [H]

Identifier: 78067614

GI number: 78067614

Start: 3288356

End: 3289291

Strand: Direct

Name: rpoH [H]

Synonym: Bcep18194_A6145

Alternate gene names: 78067614

Gene position: 3288356-3289291 (Clockwise)

Preceding gene: 78067611

Following gene: 78067615

Centisome position: 89.02

GC content: 65.38

Gene sequence:

>936_bases
GTGAGCAACGCCCTGACCCTCCCGAACACCCTGAGCCCGACGCCGGCCAAGGCCGCATCGGCAGGCTCGCTGGCGCTCGC
AGCTCAATCGATGTTGCCGGGCCAGCTTGGCAACATCGACGCGTATATCCAGGCCGTCAATCGCATTCCGCTGCTGACGG
TCGAAGAGGAACGCCAGTACGCGACCGAATTCCGCGAAGGCAACAACCTCGACTCGGCACGCCGCCTCGTGCTGTCGCAC
CTGCGGCTCGTCGTGTCGATCTCGCGCAACTATCTCGGCTACGGCCTGCCGCACGGCGACTTGATCCAGGAAGGCAACAT
CGGCCTGATGAAGGCCGTGAAGCGCTTCGATCCGGCCCAGAACGTGCGTCTCGTGTCGTACGCGATCCACTGGATCAAGG
CCGAGATTCACGAGTACATCCTGCGCAACTGGCGCACGGTGAAGGTCGCGACGACGAAGGCGCAGCGCAAGCTGTTCTTC
AACCTGCGCAGCCACAAGAAGGGCCTGCAGGCGATGACGCCCGAGGAAATCGACGGCCTCGCGAAGGAACTCAACGTCAA
GCGCGAAGACGTGACCGAAATGGAAACGCGCCTGTCGGGCGGCGACATCGCGCTCGAAGGGCAGGTCGAAGACGGCGAGG
AGTCGTACGCGCCGATCGCCTACCTGGCCGACTCGCACAACGAGCCGACCGCCGTGCTCGCCGCGCGTCAGCGCGACACG
CTGCAGACGGACGGCATCGCGCAAGCACTCGACGCACTCGACGCGCGCAGCCGCCGCATCATCGAGGCGCGCTGGCTGCA
CGTCGACGACGACGGCTCGGGCGGCTCGACGCTGCACGATCTCGCTGCCGAGTTCGGTGTGTCGGCGGAACGCATCCGCC
AGATCGAAGCGAGCGCCATGAAAAAGATGCGCACTGCCCTCGCCGAATACGCGTAA

Upstream 100 bases:

>100_bases
CTCTAAGGAATTAGCACTCGTCTTGCGGGAGTGCTAAGATGGCCCACGGAATTTCATTCAAGACCGGGGGTTTGTCCCTG
ATTCCAAAGGAGTTTTTTTA

Downstream 100 bases:

>100_bases
ATCGCTGCGATTTAGAGCGCTTCATCAAAAACCGCTGCCCGACCGGCAGCGGTTTTTTTTCGCCCGTATTTTCTCCTGCA
TTTCATTCGCTTCTCCGCTC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSNALTLPNTLSPTPAKAASAGSLALAAQSMLPGQLGNIDAYIQAVNRIPLLTVEEERQYATEFREGNNLDSARRLVLSH
LRLVVSISRNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQNVRLVSYAIHWIKAEIHEYILRNWRTVKVATTKAQRKLFF
NLRSHKKGLQAMTPEEIDGLAKELNVKREDVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDT
LQTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAMKKMRTALAEYA

Sequences:

>Translated_311_residues
MSNALTLPNTLSPTPAKAASAGSLALAAQSMLPGQLGNIDAYIQAVNRIPLLTVEEERQYATEFREGNNLDSARRLVLSH
LRLVVSISRNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQNVRLVSYAIHWIKAEIHEYILRNWRTVKVATTKAQRKLFF
NLRSHKKGLQAMTPEEIDGLAKELNVKREDVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDT
LQTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAMKKMRTALAEYA
>Mature_310_residues
SNALTLPNTLSPTPAKAASAGSLALAAQSMLPGQLGNIDAYIQAVNRIPLLTVEEERQYATEFREGNNLDSARRLVLSHL
RLVVSISRNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQNVRLVSYAIHWIKAEIHEYILRNWRTVKVATTKAQRKLFFN
LRSHKKGLQAMTPEEIDGLAKELNVKREDVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDTL
QTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAMKKMRTALAEYA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=281, Percent_Identity=50.1779359430605, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1789098, Length=275, Percent_Identity=32.7272727272727, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=32.3651452282158, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34340; Mature: 34209

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNALTLPNTLSPTPAKAASAGSLALAAQSMLPGQLGNIDAYIQAVNRIPLLTVEEERQY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEHHHHHH
ATEFREGNNLDSARRLVLSHLRLVVSISRNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCC
NVRLVSYAIHWIKAEIHEYILRNWRTVKVATTKAQRKLFFNLRSHKKGLQAMTPEEIDGL
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
AKELNVKREDVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDT
HHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCHHEEEEHHHCC
LQTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAM
HHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
KKMRTALAEYA
HHHHHHHHHCC
>Mature Secondary Structure 
SNALTLPNTLSPTPAKAASAGSLALAAQSMLPGQLGNIDAYIQAVNRIPLLTVEEERQY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEHHHHHH
ATEFREGNNLDSARRLVLSHLRLVVSISRNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCC
NVRLVSYAIHWIKAEIHEYILRNWRTVKVATTKAQRKLFFNLRSHKKGLQAMTPEEIDGL
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
AKELNVKREDVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDT
HHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCHHEEEEHHHCC
LQTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAM
HHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
KKMRTALAEYA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]