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The map label for this gene is pykA [H]

Identifier: 78067427

GI number: 78067427

Start: 3087775

End: 3089211

Strand: Reverse

Name: pykA [H]

Synonym: Bcep18194_A5958

Alternate gene names: 78067427

Gene position: 3089211-3087775 (Counterclockwise)

Preceding gene: 78067428

Following gene: 78067426

Centisome position: 83.62

GC content: 67.29

Gene sequence:

>1437_bases
ATGCAGCGCGCCACCAAGATAGTCGCCACGATCGGCCCGGCTTCCAGTTCGCCGGAGATTCTGCTGCAGATGATGCAGGC
GGGCCTCGACGTCGTGCGGCTCAATTTTTCGCACGGCACGGCCGACGATCACCGCCAGCGCGCCGAGATGGTGCGCGAGG
CCGCCCGCAAGGTCGGCCGCGAGATCGCGATCATGGCCGACCTCCAGGGCCCGAAGATCCGCGTCGGCAAGTTCGAGAAC
GGCAAGACGACGCTCGAGCCGGGCCAGGCCTTCATCCTCGACGCGGGCTGCGAGCTGGGTAACGACGAACGCGTCGGCCT
CGACTACAAGGAACTGCCGCGCGACCTGCGCACGGGCGACCTGCTGCTGCTGAACGACGGCCTGATCGTGCTGACCGTCG
AGCGCGTGCTCGGCGACGAGATCCACACGATCGTCAAGGTGGGCGGCGAGCTGTCGAACAACAAGGGCATCAACCGCCAG
GGCGGCGGCCTGTCGGCGCCCGCGCTGACCGCGAAGGACATGGAAGACATCCGCACCGCGATGTCGCTCGGCGCGGACCT
GGTGGCGGTGTCGTTCCCGAAGAACGCGACCGACATGGAAATGGCGCGCCAGCTCGCGAACATCGCGGGCGCGCCGTACG
GCATCAAGCCGAAAATGATCGCGAAGATCGAGCGCGCGGAAGCGATCCCGGCGCTGCAAAGCATTCTCGACGCGTCCGAC
GGCATCATGGTCGCGCGTGGCGACCTCGCGGTGGAAGTGGGCAACGCGGCCGTGCCGGCACTGCAGAAGCGGATGATCCG
GATGGCGCGCGAGTCGAACAAGCTCGTGATCACCGCGACGCAGATGATGGAATCGATGATCCACGCACCCGTGCCGACCC
GCGCGGAAGTGTCGGACGTCGCGAACGCGGTGCTCGACGGCACCGACGCGGTGATGCTGTCGGCCGAGACGGCCGCCGGC
AAGTATCCGGTCGTGACGATCGAGACGATGGCGGCCGTGTGCGTCGAGGCGGAAAAGTCGGAGCACGTCGAGCTGGACAA
GGATTTCCTCGACCGCACGTTCACGCGGATCGACCAGTCGATCGCGATGGGCGCGCTGTTCACCGCGTACCACCTGGGTG
CGAAGGCGATCATCGCGCTGACCGAATCGGGCGCGACCGCGCTGTGGATGTCGCGCCACTACACGCACGTGCCGATCTTT
GCGCTGACGCCGCGCGTCGGCAGCGAGCGCACGATGGCGCTGTACCGCAACGTGACGCCGCTGCACGTCGACTTCAACAG
CGACCGCGACTCGGCGCTGCAGGCGGCGCTCGAGATCGTCGTCAAGCAGGGCTACGTGCAGCACGGCGACATGGTCGTGC
TGACCGTCGGCGAGCCGATGGGGCAGGCGGGTGGCACCAACACGCTGAAGATCGTGCGGGTCGGCGAGCACTATTGA

Upstream 100 bases:

>100_bases
CACCGGCACGCCCCCCGGAACGGCCGCCGCTGGGCCCGCCGTTTCCGGCTCTCGCAGCGTTTCACCCAGCGCATCCACCT
TGATCCAGATGAGGAGTTTC

Downstream 100 bases:

>100_bases
GCGCCGACTGAGCATCGGGCCGGCCTGCGGGCCGGCCGCCCGGATCGGCTGAAAAAAGCGATCCGGCACCGTTTTTCATG
CGAAAGCCGCGTGGGGTCCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MQRATKIVATIGPASSSPEILLQMMQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGREIAIMADLQGPKIRVGKFEN
GKTTLEPGQAFILDAGCELGNDERVGLDYKELPRDLRTGDLLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQSILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAETAAG
KYPVVTIETMAAVCVEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIIALTESGATALWMSRHYTHVPIF
ALTPRVGSERTMALYRNVTPLHVDFNSDRDSALQAALEIVVKQGYVQHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY

Sequences:

>Translated_478_residues
MQRATKIVATIGPASSSPEILLQMMQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGREIAIMADLQGPKIRVGKFEN
GKTTLEPGQAFILDAGCELGNDERVGLDYKELPRDLRTGDLLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQSILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAETAAG
KYPVVTIETMAAVCVEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIIALTESGATALWMSRHYTHVPIF
ALTPRVGSERTMALYRNVTPLHVDFNSDRDSALQAALEIVVKQGYVQHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY
>Mature_478_residues
MQRATKIVATIGPASSSPEILLQMMQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGREIAIMADLQGPKIRVGKFEN
GKTTLEPGQAFILDAGCELGNDERVGLDYKELPRDLRTGDLLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQSILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAETAAG
KYPVVTIETMAAVCVEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIIALTESGATALWMSRHYTHVPIF
ALTPRVGSERTMALYRNVTPLHVDFNSDRDSALQAALEIVVKQGYVQHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=493, Percent_Identity=36.7139959432049, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI33286420, Length=493, Percent_Identity=36.7139959432049, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=36.3083164300203, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=36.8951612903226, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=36.8951612903226, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=36.0824742268041, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=36.0824742268041, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=33.4728033472803, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=33.4728033472803, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=31.1926605504587, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=52.5987525987526, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=38.6411889596603, Blast_Score=303, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=36.7768595041322, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=38.6036960985626, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=38.6036960985626, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=38.8090349075975, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=38.8090349075975, Blast_Score=266, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=34.7022587268994, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324923, Length=492, Percent_Identity=35.9756097560976, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=38.7423935091278, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=38.7423935091278, Blast_Score=285, Evalue=6e-77,
Organism=Drosophila melanogaster, GI24648966, Length=448, Percent_Identity=35.9375, Blast_Score=235, Evalue=6e-62,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=32.1063394683027, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=36.0294117647059, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51428; Mature: 51428

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRATKIVATIGPASSSPEILLQMMQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGR
CCCHHEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
EIAIMADLQGPKIRVGKFENGKTTLEPGQAFILDAGCELGNDERVGLDYKELPRDLRTGD
EEEEEEECCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHCHHCCCCC
LLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQGGGLSAPALTAKDMEDIRTA
EEEEECCEEEEEEHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQSILDASD
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDV
CEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
ANAVLDGTDAVMLSAETAAGKYPVVTIETMAAVCVEAEKSEHVELDKDFLDRTFTRIDQS
HHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
IAMGALFTAYHLGAKAIIALTESGATALWMSRHYTHVPIFALTPRVGSERTMALYRNVTP
HHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHCCCCE
LHVDFNSDRDSALQAALEIVVKQGYVQHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY
EEEECCCCHHHHHHHHHHHHHHHCCEECCCEEEEECCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MQRATKIVATIGPASSSPEILLQMMQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGR
CCCHHEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
EIAIMADLQGPKIRVGKFENGKTTLEPGQAFILDAGCELGNDERVGLDYKELPRDLRTGD
EEEEEEECCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHCHHCCCCC
LLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQGGGLSAPALTAKDMEDIRTA
EEEEECCEEEEEEHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQSILDASD
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDV
CEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
ANAVLDGTDAVMLSAETAAGKYPVVTIETMAAVCVEAEKSEHVELDKDFLDRTFTRIDQS
HHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
IAMGALFTAYHLGAKAIIALTESGATALWMSRHYTHVPIFALTPRVGSERTMALYRNVTP
HHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHCCCCE
LHVDFNSDRDSALQAALEIVVKQGYVQHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY
EEEECCCCHHHHHHHHHHHHHHHCCEECCCEEEEECCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]