Click here to switch to the map view.

The map label for this gene is pgk

Identifier: 78067428

GI number: 78067428

Start: 3089496

End: 3090692

Strand: Reverse

Name: pgk

Synonym: Bcep18194_A5959

Alternate gene names: 78067428

Gene position: 3090692-3089496 (Counterclockwise)

Preceding gene: 78067429

Following gene: 78067427

Centisome position: 83.67

GC content: 68.17

Gene sequence:

>1197_bases
ATGAGCCAAGTCAAGCGTCTTACCGACCTGATCGCCGCCGGCCAGCTCGCCGGCAAGCGGGTATTCATCCGCGCCGACCT
GAACGTCCCGCAGGACGACCAGGGCAACATCACCGAAGACACGCGCGTGCGCGCGTCCGTGCCGGCCATCCAGGCTGCGC
TCGACGCCGGCGCGGCCGTGATGGTCACGTCGCACCTCGGTCGCCCGACCGAAGGCGAATTCAAGCCGGAAGATTCGCTC
GCGCCGGTCGCGAAGCGCCTCGCCGAGCTGCTCGGCCGTGACGTGCCGCTCGTGTCGAACTGGGTCGAGAACGGCGTGAA
CGTCGCGCCGGGCCAGGTCGTACTGCTCGAGAACTGCCGCGTGAACAAGGGCGAGAAGAAGAATTCGGACGAGCTCGCGC
AGAAGATGGCGAAGCTCTGCGACGTGTACGTGAACGACGCGTTCGGCACCGCGCACCGCGCGGAAGCGACCACGCACGGG
ATCGCGAAGTACGCGCCGGTCGCGTGCGCGGGCCCGCTGCTGGCCGCCGAACTCGACGCGCTCGGCAAGGCGCTCGGCAA
CCCGGCCCGCCCGCTGGTGGCGATCGTCGCCGGCTCGAAGGTATCGACCAAGCTCACGATCCTGAAGTCGCTGGCCGGCA
AGGTCGACCAGCTGATCGTCGGCGGCGGCATCGCGAACACGTTCATGCTCGCGGCCGGCCTGTCGATCGGCAAGTCGCTC
GCGGAAGCCGACCTCGTCAACGAGGCGAAGGCGATCATCGACGAAGCACGCGAGCGCGGCGCATCGGTGCCGATCCCGAC
CGACGTCGTGACGGCCAAGGAATTCTCGCCGACGGCTGTCGCCACGGTGAAGCAGGTCGCCGACATCGAAGCGGACGACA
TGATCCTCGACATCGGCCCCGATACGGCCAAGGCGCTCGCGAGCCAGCTCGAGAAGGCCGGCACGATCGTGTGGAACGGC
CCGGTCGGCGTGTTCGAGTTCGACCAGTTCGGCAACGGCACCAAGACGCTCGCGGAAGCGATCGCCAAATCGTCCGCGTT
CTCGATCGCGGGCGGCGGCGACACGCTCGCGGCCATCGCCAAGTACGGCATCCATGACCAGGTCAGCTACATCTCGACGG
GCGGCGGCGCCTTCCTCGAGTTCCTCGAGGGGAAGAAGCTGCCGGCGGTGGAAGTGCTGGAAACGCGGGCGGCCTGA

Upstream 100 bases:

>100_bases
CGAACCGCCAGCCACGGCCGCATCCGGCGCCCTTTCGCGGCAGCCCGCGCACGTCCAGCGTGAACCCCCTTTCCCCATCC
ACTACTTCAATTTGTCCAAC

Downstream 100 bases:

>100_bases
GCGTGGCGGCGCGCGCAGGGGTGATGAAGGCCGCGCGCTCCGTGAAAGCGGAGCAGCCGGCCGGCGCGGGCAAGCGCAAA
CGCGCGCCCGCGCGGGCGAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSQVKRLTDLIAAGQLAGKRVFIRADLNVPQDDQGNITEDTRVRASVPAIQAALDAGAAVMVTSHLGRPTEGEFKPEDSL
APVAKRLAELLGRDVPLVSNWVENGVNVAPGQVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHG
IAKYAPVACAGPLLAAELDALGKALGNPARPLVAIVAGSKVSTKLTILKSLAGKVDQLIVGGGIANTFMLAAGLSIGKSL
AEADLVNEAKAIIDEARERGASVPIPTDVVTAKEFSPTAVATVKQVADIEADDMILDIGPDTAKALASQLEKAGTIVWNG
PVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIAKYGIHDQVSYISTGGGAFLEFLEGKKLPAVEVLETRAA

Sequences:

>Translated_398_residues
MSQVKRLTDLIAAGQLAGKRVFIRADLNVPQDDQGNITEDTRVRASVPAIQAALDAGAAVMVTSHLGRPTEGEFKPEDSL
APVAKRLAELLGRDVPLVSNWVENGVNVAPGQVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHG
IAKYAPVACAGPLLAAELDALGKALGNPARPLVAIVAGSKVSTKLTILKSLAGKVDQLIVGGGIANTFMLAAGLSIGKSL
AEADLVNEAKAIIDEARERGASVPIPTDVVTAKEFSPTAVATVKQVADIEADDMILDIGPDTAKALASQLEKAGTIVWNG
PVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIAKYGIHDQVSYISTGGGAFLEFLEGKKLPAVEVLETRAA
>Mature_397_residues
SQVKRLTDLIAAGQLAGKRVFIRADLNVPQDDQGNITEDTRVRASVPAIQAALDAGAAVMVTSHLGRPTEGEFKPEDSLA
PVAKRLAELLGRDVPLVSNWVENGVNVAPGQVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGI
AKYAPVACAGPLLAAELDALGKALGNPARPLVAIVAGSKVSTKLTILKSLAGKVDQLIVGGGIANTFMLAAGLSIGKSLA
EADLVNEAKAIIDEARERGASVPIPTDVVTAKEFSPTAVATVKQVADIEADDMILDIGPDTAKALASQLEKAGTIVWNGP
VGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIAKYGIHDQVSYISTGGGAFLEFLEGKKLPAVEVLETRAA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=421, Percent_Identity=42.0427553444181, Blast_Score=283, Evalue=1e-76,
Organism=Homo sapiens, GI4505763, Length=402, Percent_Identity=40.7960199004975, Blast_Score=272, Evalue=5e-73,
Organism=Escherichia coli, GI1789294, Length=397, Percent_Identity=63.727959697733, Blast_Score=475, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17508823, Length=422, Percent_Identity=43.8388625592417, Blast_Score=300, Evalue=6e-82,
Organism=Saccharomyces cerevisiae, GI6319857, Length=408, Percent_Identity=42.8921568627451, Blast_Score=281, Evalue=1e-76,
Organism=Drosophila melanogaster, GI17136394, Length=406, Percent_Identity=40.3940886699507, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24581203, Length=421, Percent_Identity=38.7173396674584, Blast_Score=248, Evalue=7e-66,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41268; Mature: 41136

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVKRLTDLIAAGQLAGKRVFIRADLNVPQDDQGNITEDTRVRASVPAIQAALDAGAAV
CCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEE
MVTSHLGRPTEGEFKPEDSLAPVAKRLAELLGRDVPLVSNWVENGVNVAPGQVVLLENCR
EEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEECCC
VNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGIAKYAPVACAGPLLAAELDA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
LGKALGNPARPLVAIVAGSKVSTKLTILKSLAGKVDQLIVGGGIANTFMLAAGLSIGKSL
HHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHH
AEADLVNEAKAIIDEARERGASVPIPTDVVTAKEFSPTAVATVKQVADIEADDMILDIGP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCC
DTAKALASQLEKAGTIVWNGPVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIA
CHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHH
KYGIHDQVSYISTGGGAFLEFLEGKKLPAVEVLETRAA
HHCCCHHHHHEECCCHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SQVKRLTDLIAAGQLAGKRVFIRADLNVPQDDQGNITEDTRVRASVPAIQAALDAGAAV
CHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEE
MVTSHLGRPTEGEFKPEDSLAPVAKRLAELLGRDVPLVSNWVENGVNVAPGQVVLLENCR
EEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEECCC
VNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGIAKYAPVACAGPLLAAELDA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
LGKALGNPARPLVAIVAGSKVSTKLTILKSLAGKVDQLIVGGGIANTFMLAAGLSIGKSL
HHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHH
AEADLVNEAKAIIDEARERGASVPIPTDVVTAKEFSPTAVATVKQVADIEADDMILDIGP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCC
DTAKALASQLEKAGTIVWNGPVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIA
CHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHH
KYGIHDQVSYISTGGGAFLEFLEGKKLPAVEVLETRAA
HHCCCHHHHHEECCCHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]