Click here to switch to the map view.

The map label for this gene is mexA [H]

Identifier: 78067409

GI number: 78067409

Start: 3068366

End: 3069643

Strand: Reverse

Name: mexA [H]

Synonym: Bcep18194_A5940

Alternate gene names: 78067409

Gene position: 3069643-3068366 (Counterclockwise)

Preceding gene: 78067411

Following gene: 78067408

Centisome position: 83.1

GC content: 68.54

Gene sequence:

>1278_bases
ATGCGCGTCGAACGGGTTCCATACCGCTTAATCACTGTCGCGACGGCCGCCGTTTTCCTGGCCGCGTGCGGGAAAAAAGA
ATCGGCACCGCCGCCGCAAACGCCGGAAGTCGGCGTCGTCACCGTCCAACCGCAATCCGTACCGGTCTTCACCGACCTGC
CGGGCCGCACCAGTGCATTCCTCGTCGCGCAGGTCCGCGCGCGGGTCGACGGCATCGTGCTGCGCCGTGAATTCACCGAA
GGCACCGACGTCAAGGCCGGTCAGCGCCTCTACAAGATCGACCCGGCGCCGTACGTCGCCGCGCTGAACAGCGCGAAGGC
GACGCTCGCGAAGGCGCAGGCGAACCTCGTCACGCAGAACGCGCTGGTCGCGCGCTACAAGGTGCTGGTGGCCGCGAACG
CGGTCAGCAAGCAGGACTACGACAACGCGGTGGCCACGCAAGGCCAGGCCGCGGCGGATGTCGCGTCCGGCAAGGCAGCG
GTCGACACCGCGCAGATCAACCTCGGCTACACCGATGTCGTGTCGCCGATCACCGGCCGCGTCGGCATCTCGCAAGTGAC
GCCGGGCGCCTACGTGCAGGCGAGCCAGGCGACGCTGATGTCGACGGTTCAGCAGCTCGATCCGGTGTACGTGGACCTCA
CCCAATCGAGCCTCGACGGGCTGAAGCTGCGTCAGGACGTGCAGAGCGGCCGCCTGAAGACGACTGGCCCGGGCGCGGCG
AAGGTGTCGCTGATCCTGGAAGACGGCAAGACCTATTCGGAACCGGGCAAGCTGCAGTTCTCGGACGTGACGGTCGACCA
GACCACTGGCTCGGTGACGATCCGCGCGGTGTTCCCGAACCCGGGCCGCGTGCTGCTGCCGGGCATGTTCGTGCGCGCAC
GGATCGAGGAAGGCGTGAACGAGAACGCGTTCCTGGTGCCGCAGATCGGCGTCACGCATGACCAGAAGGGCCAGGCGATC
GCGATGGTGGTGAACGCCAGCAACAAGGTCGAGCCGCGTCCGCTGAAGACGACGGGCATGCAAGGTGCGAACTGGATCGT
CGAAGGCGGTCTGGAAGCGGGCGATCACGTGATCGTGCAGGGCGTCGACAAGGTGCGCCCGGGTGCGACCGTGAAGACGG
TCGCCGCGCAGCTCGCCCCGGCGGCCGATGCCGCATCGGGTGCTGCTGCCGCGTCCGCCGCGCCGGCTGCCGCCGGTTCC
GGCGCCGCTGCCGCTTCCGGTGCTGCCGCATCGGGCGCTGCGCCGGCGAGTGCTGCCGCTGCTTCGAGCGCGCAATAA

Upstream 100 bases:

>100_bases
TATTTCAGGTGTTCGATCTGCGCCGTGAGGCGCATCCGTGTTGCACTCCCTCCGGTCGGGTGCGGCACTTATTCCATTGG
TTATACACAGAGGTCGCTCC

Downstream 100 bases:

>100_bases
CAGGGAGCCTGTTTCATGGCAAAGTTTTTTATCGATCGCCCGATCTTCGCGTGGGTGATCGCCATCATTCTGATGCTGGC
CGGGGTCGCGTCGATCTTCA

Product: HlyD family secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQSVPVFTDLPGRTSAFLVAQVRARVDGIVLRREFTE
GTDVKAGQRLYKIDPAPYVAALNSAKATLAKAQANLVTQNALVARYKVLVAANAVSKQDYDNAVATQGQAAADVASGKAA
VDTAQINLGYTDVVSPITGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDVQSGRLKTTGPGAA
KVSLILEDGKTYSEPGKLQFSDVTVDQTTGSVTIRAVFPNPGRVLLPGMFVRARIEEGVNENAFLVPQIGVTHDQKGQAI
AMVVNASNKVEPRPLKTTGMQGANWIVEGGLEAGDHVIVQGVDKVRPGATVKTVAAQLAPAADAASGAAAASAAPAAAGS
GAAAASGAAASGAAPASAAAASSAQ

Sequences:

>Translated_425_residues
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQSVPVFTDLPGRTSAFLVAQVRARVDGIVLRREFTE
GTDVKAGQRLYKIDPAPYVAALNSAKATLAKAQANLVTQNALVARYKVLVAANAVSKQDYDNAVATQGQAAADVASGKAA
VDTAQINLGYTDVVSPITGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDVQSGRLKTTGPGAA
KVSLILEDGKTYSEPGKLQFSDVTVDQTTGSVTIRAVFPNPGRVLLPGMFVRARIEEGVNENAFLVPQIGVTHDQKGQAI
AMVVNASNKVEPRPLKTTGMQGANWIVEGGLEAGDHVIVQGVDKVRPGATVKTVAAQLAPAADAASGAAAASAAPAAAGS
GAAAASGAAASGAAPASAAAASSAQ
>Mature_425_residues
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQSVPVFTDLPGRTSAFLVAQVRARVDGIVLRREFTE
GTDVKAGQRLYKIDPAPYVAALNSAKATLAKAQANLVTQNALVARYKVLVAANAVSKQDYDNAVATQGQAAADVASGKAA
VDTAQINLGYTDVVSPITGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDVQSGRLKTTGPGAA
KVSLILEDGKTYSEPGKLQFSDVTVDQTTGSVTIRAVFPNPGRVLLPGMFVRARIEEGVNENAFLVPQIGVTHDQKGQAI
AMVVNASNKVEPRPLKTTGMQGANWIVEGGLEAGDHVIVQGVDKVRPGATVKTVAAQLAPAADAASGAAAASAAPAAAGS
GAAAASGAAASGAAPASAAAASSAQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=383, Percent_Identity=52.2193211488251, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789665, Length=361, Percent_Identity=49.584487534626, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI1789929, Length=369, Percent_Identity=48.2384823848238, Blast_Score=332, Evalue=4e-92,
Organism=Escherichia coli, GI87082036, Length=364, Percent_Identity=31.5934065934066, Blast_Score=152, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43370; Mature: 43370

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQSVPVFTDLPGRTSAF
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCHHH
LVAQVRARVDGIVLRREFTEGTDVKAGQRLYKIDPAPYVAALNSAKATLAKAQANLVTQN
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCCEEHHCCHHHHHHHHHHHHHHHH
ALVARYKVLVAANAVSKQDYDNAVATQGQAAADVASGKAAVDTAQINLGYTDVVSPITGR
HHHHHEEEEEEECHHCCCCCCCHHHCCCCHHHHHCCCCEEEEEEEEECCHHHHHCCCCCC
VGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDVQSGRLKTTGPGAA
CCCCCCCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEECCCCCE
KVSLILEDGKTYSEPGKLQFSDVTVDQTTGSVTIRAVFPNPGRVLLPGMFVRARIEEGVN
EEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCEECCCHHHHHHHHCCCC
ENAFLVPQIGVTHDQKGQAIAMVVNASNKVEPRPLKTTGMQGANWIVEGGLEAGDHVIVQ
CCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEE
GVDKVRPGATVKTVAAQLAPAADAASGAAAASAAPAAAGSGAAAASGAAASGAAPASAAA
CHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
ASSAQ
CCCCC
>Mature Secondary Structure
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQSVPVFTDLPGRTSAF
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCHHH
LVAQVRARVDGIVLRREFTEGTDVKAGQRLYKIDPAPYVAALNSAKATLAKAQANLVTQN
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCCEEHHCCHHHHHHHHHHHHHHHH
ALVARYKVLVAANAVSKQDYDNAVATQGQAAADVASGKAAVDTAQINLGYTDVVSPITGR
HHHHHEEEEEEECHHCCCCCCCHHHCCCCHHHHHCCCCEEEEEEEEECCHHHHHCCCCCC
VGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDVQSGRLKTTGPGAA
CCCCCCCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEECCCCCE
KVSLILEDGKTYSEPGKLQFSDVTVDQTTGSVTIRAVFPNPGRVLLPGMFVRARIEEGVN
EEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCEECCCHHHHHHHHCCCC
ENAFLVPQIGVTHDQKGQAIAMVVNASNKVEPRPLKTTGMQGANWIVEGGLEAGDHVIVQ
CCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEE
GVDKVRPGATVKTVAAQLAPAADAASGAAAASAAPAAAGSGAAAASGAAASGAAPASAAA
CHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
ASSAQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]