Click here to switch to the map view.

The map label for this gene is rsuA [H]

Identifier: 78067288

GI number: 78067288

Start: 2934508

End: 2935224

Strand: Direct

Name: rsuA [H]

Synonym: Bcep18194_A5819

Alternate gene names: 78067288

Gene position: 2934508-2935224 (Clockwise)

Preceding gene: 78067283

Following gene: 78067289

Centisome position: 79.44

GC content: 68.2

Gene sequence:

>717_bases
ATGGATCTCGAAAGCATTCTCTACACCCAGGGCTTCGGCTCGCGCCGCCAGTGCCGCGGCCTGATCGAAGCAGGCCGCGT
CACCGTGGCGGGCGCCAGCGCGACCGACCCCGACGCCACGTTCGATACCGACGGCCTCGTGTTCTCGGTCGACGATACGG
CGTGGCCGTTCCACGCGCATGCATATCTCGCGCTCAACAAGCCGGCCGGCTACGAGTGTTCGCGCGATCCGCAACACCAC
GCGAGCGTGTTCAGCCTGCTGCCCGCACCGCTCGTCGCGCGCGGCGTGCAGTGCGTCGGACGTCTCGACCAGGACACGAC
GGGCTTGCTGCTGCTGTCCGACGACGGCCAGTTCGTGCACGCGTACACGTCGCCGAAGCGCAAGGTACCGAAGACCTACG
TGGCGACCGTGCGCCACCCGCTCGACGACACGCAGTTGAACACGCTGCGCACCGGCGTGCAGCTCCACGGCGAGCCGAAG
CCGATCGCGGCGGTCGCCGCCGACGCGCGCGACACGCACGCGCTCGCGCTGACCGTCCTCGAAGGCAAGTATCACCAGGT
CAAGCGCATGGTCGCGGCGGCGAGCAATCGCGTCGAAGCGCTGCATCGCGAAAGCATCGGCGGCTTCACACTGCCGGGCG
ATCTCGCACCGGGTGCGTGGCGCTGGCTCGATGACGCCGACCTTTCAGCACTGCGCAATCCCGTCGAAACCCTGTAA

Upstream 100 bases:

>100_bases
GAATGAACGGGCGCCGCGCCCCGCGCGGCGTACCGGCAGCTTACCAGCGCATGCACGTTCATGCCGGTCGCGCGCCATCG
CTTGATAGAATGCGCGCGCT

Downstream 100 bases:

>100_bases
GGGAAAATCCGCACGACCGTTCGACGTCCAGCCGCATCTCGGCCGCATGTTCCCCCGCTATACTGAAATCACAAGCTGTA
AGAGAACAGCGACGTAGGGG

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MDLESILYTQGFGSRRQCRGLIEAGRVTVAGASATDPDATFDTDGLVFSVDDTAWPFHAHAYLALNKPAGYECSRDPQHH
ASVFSLLPAPLVARGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDTQLNTLRTGVQLHGEPK
PIAAVAADARDTHALALTVLEGKYHQVKRMVAAASNRVEALHRESIGGFTLPGDLAPGAWRWLDDADLSALRNPVETL

Sequences:

>Translated_238_residues
MDLESILYTQGFGSRRQCRGLIEAGRVTVAGASATDPDATFDTDGLVFSVDDTAWPFHAHAYLALNKPAGYECSRDPQHH
ASVFSLLPAPLVARGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDTQLNTLRTGVQLHGEPK
PIAAVAADARDTHALALTVLEGKYHQVKRMVAAASNRVEALHRESIGGFTLPGDLAPGAWRWLDDADLSALRNPVETL
>Mature_238_residues
MDLESILYTQGFGSRRQCRGLIEAGRVTVAGASATDPDATFDTDGLVFSVDDTAWPFHAHAYLALNKPAGYECSRDPQHH
ASVFSLLPAPLVARGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDTQLNTLRTGVQLHGEPK
PIAAVAADARDTHALALTVLEGKYHQVKRMVAAASNRVEALHRESIGGFTLPGDLAPGAWRWLDDADLSALRNPVETL

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=239, Percent_Identity=38.4937238493724, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1787524, Length=242, Percent_Identity=35.1239669421488, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790453, Length=238, Percent_Identity=26.890756302521, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI87081838, Length=150, Percent_Identity=31.3333333333333, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 25758; Mature: 25758

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLESILYTQGFGSRRQCRGLIEAGRVTVAGASATDPDATFDTDGLVFSVDDTAWPFHAH
CCHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEE
AYLALNKPAGYECSRDPQHHASVFSLLPAPLVARGVQCVGRLDQDTTGLLLLSDDGQFVH
EEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEE
AYTSPKRKVPKTYVATVRHPLDDTQLNTLRTGVQLHGEPKPIAAVAADARDTHALALTVL
EECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCEEECCCCCCEEEEECCCCCCEEEEEEEE
EGKYHQVKRMVAAASNRVEALHRESIGGFTLPGDLAPGAWRWLDDADLSALRNPVETL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCEECCCCCCHHHHCCHHHCC
>Mature Secondary Structure
MDLESILYTQGFGSRRQCRGLIEAGRVTVAGASATDPDATFDTDGLVFSVDDTAWPFHAH
CCHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEE
AYLALNKPAGYECSRDPQHHASVFSLLPAPLVARGVQCVGRLDQDTTGLLLLSDDGQFVH
EEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEE
AYTSPKRKVPKTYVATVRHPLDDTQLNTLRTGVQLHGEPKPIAAVAADARDTHALALTVL
EECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCEEECCCCCCEEEEECCCCCCEEEEEEEE
EGKYHQVKRMVAAASNRVEALHRESIGGFTLPGDLAPGAWRWLDDADLSALRNPVETL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCEECCCCCCHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]